Publication Information

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4153, 4161, 4178, 4208, 4213, 4220, 4232, 4252, 4274, 4311, 4326, 4355, 4358, 4369, 4390, 4398, 4421, 4424, 4430, 4440, 4449, 4471, 4475], 'phosphodiester': [6, 252, 554, 1255, 2189], 'bond': [7, 253, 1256, 1282, 2190, 2441, 3242, 3698], 'between': [8, 254, 556, 1257, 3159, 3372, 3440], 'a': [9, 14, 54, 60, 66, 70, 84, 176, 185, 201, 225, 236, 255, 260, 300, 306, 312, 316, 330, 422, 431, 447, 471, 482, 561, 564, 608, 1049, 1099, 1232, 1246, 1280, 1413, 1428, 1437, 1440, 1443, 1452, 1688, 1804, 1942, 1959, 1970, 2023, 2179, 2228, 2246, 2353, 2439, 2662, 2668, 2713, 2811, 2816, 2912, 2982, 2986, 3057, 3062, 3137, 3147, 3187, 3303, 3330, 3425, 3514, 3544, 3675, 3683, 3696, 3738, 3769, 3847, 3884, 3971, 3990, 4067, 4075, 4093, 4096, 4129, 4166, 4245], 'DNA': [10, 67, 151, 178, 242, 244, 256, 313, 397, 424, 488, 490, 565, 707, 714, 720, 738, 768, 976, 1263, 1278, 1438, 1930, 2198, 2455, 2664, 2878, 2965, 3004, 3026, 3052, 3083, 3104, 3374, 3438, 3444, 3689, 4073, 4442], '3′': [11, 202, 239, 257, 448, 485, 763, 1274, 1952, 2195, 2679, 2954, 3067, 3102, 3163, 3313], 'end': [12, 193, 258, 439, 764, 1275, 1953, 2196, 2680, 2955, 3068, 3164, 3314], 'and': [13, 32, 82, 131, 170, 179, 205, 259, 278, 328, 377, 416, 425, 451, 563, 635, 706, 716, 755, 893, 1205, 1261, 1436, 1824, 1908, 2289, 2364, 2496, 2623, 2632, 2756, 2792, 2806, 2861, 2907, 2968, 3020, 3110, 3161, 3171, 3176, 3200, 3327, 3352, 3375, 3442, 3520, 3581, 3609, 3660, 3668, 3677, 3715, 3766, 3775, 3893, 3909, 3947, 3965, 4007, 4053, 4114, 4160, 4207, 4273, 4299, 4445], 'tyrosyl': [15, 261], 'moiety.': [16, 262], 'In': [17, 163, 213, 263, 409, 459, 1224, 1956, 2172, 2267, 3130, 3360, 4060], 'eukaryotic': [18, 264, 913], 'cells,': [19, 265], 'this': [20, 75, 266, 321, 1096, 1403, 1671, 1792, 1902, 1909, 2341, 3208, 3318, 3361], 'type': [21, 141, 267, 387, 517, 540, 596, 609], 'of': [22, 40, 101, 124, 139, 157, 194, 238, 268, 286, 347, 370, 385, 403, 440, 484, 501, 524, 560, 597, 601, 615, 747, 765, 832, 835, 878, 907, 1051, 1095, 1101, 1115, 1219, 1228, 1276, 1287, 1402, 1454, 1640, 1676, 1690, 1733, 1744, 1807, 1816, 1849, 1901, 1944, 1954, 2197, 2248, 2299, 2490, 2498, 2510, 2556, 2658, 2721, 2769, 2795, 2830, 2835, 2875, 2915, 2933, 2945, 2956, 2961, 2976, 2991, 3154, 3165, 3198, 3207, 3215, 3220, 3238, 3251, 3256, 3271, 3302, 3317, 3333, 3393, 3400, 3406, 3422, 3428, 3546, 3549, 3559, 3595, 3614, 3639, 3712, 3725, 3751, 3800, 3809, 3814, 3824, 3840, 3850, 3855, 3865, 3867, 3873, 3887, 3929, 3942, 3945, 3961, 3977, 3981, 3992, 3999, 4085, 4088, 4131, 4185, 4192, 4198, 4215, 4219, 4234, 4251, 4261, 4290, 4307, 4313, 4316, 4321, 4357, 4365, 4372, 4400, 4420, 4423, 4432, 4448, 4470], 'linkage': [23, 76, 269, 322, 555, 598, 1924, 2355, 2732, 3158, 3371, 3439], 'is': [24, 63, 117, 188, 270, 309, 363, 434, 599, 753, 902, 1048, 1058, 1241, 1406, 1417, 1554, 1642, 1874, 1889, 1904, 1917, 2059, 2231, 2351, 2433, 2444, 2774, 3075, 3086, 3380], 'found': [25, 271], 'in': [26, 37, 43, 58, 183, 272, 283, 289, 304, 429, 627, 744, 875, 904, 912, 1112, 1235, 1253, 1445, 1451, 1513, 1693, 1832, 1843, 1865, 1931, 2030, 2068, 2294, 2456, 2470, 2487, 2627, 2810, 2838, 2881, 2989, 3077, 3088, 3195, 3217, 3243, 3368, 3403, 3434, 3498, 3522, 3575, 3631, 3686, 3730, 3811, 3883, 3890, 3922, 3931, 3939, 3996, 4157, 4195, 4244, 4266, 4310, 4345, 4429, 4463], 'stalled': [27, 146, 273, 392, 881, 2057], 'topoisomerase': [28, 85, 274, 331, 503, 526, 611, 710, 757, 882, 908, 1726, 1825, 2016, 2058, 2491, 2672, 2716, 2735, 2771, 2820, 3189, 3273, 3297, 3308, 3335, 3377, 4005, 4077], 'I-DNA': [29, 86, 275, 332, 909, 1727, 1826, 2017, 2492, 3274, 3298, 3336, 4006], 'covalent': [30, 55, 135, 276, 301, 381, 603, 910, 1678, 1719, 1725, 1933, 2348, 2793, 2883, 3246, 3259, 3275, 3386, 3796, 4009, 4017, 4126, 4180, 4237, 4359], 'complexes,': [31, 277], 'Tdp1': [33, 50, 102, 142, 173, 198, 220, 231, 279, 296, 348, 388, 419, 444, 466, 477, 901, 1230, 1431, 1720, 1734, 1746, 1893, 1906, 1916, 1927, 1939, 1963, 2029, 2066, 2182, 2276, 2300, 2432, 2451, 2463, 2468, 2478, 2499, 2616, 2659, 2836, 2886, 3160, 3257, 3401, 3441, 3713, 3729, 3788, 3875, 3880, 4012, 4039, 4090, 4101, 4112, 4149, 4179, 4193, 4236, 4265, 4373, 4426, 4472], 'has': [34, 280, 1214, 1458, 1936, 2019, 2052, 2183, 2565, 3015, 3056], 'been': [35, 281, 1215, 1459, 2053, 2184, 2279, 2358, 2566], 'implicated': [36, 282], 'repair': [39, 285, 906, 2489], 'such': [41, 287, 631, 879, 1107, 2281], 'complexes': [42, 288, 1118, 1827, 1861, 3276, 4018], 'vivo.': [44, 290], 'We': [45, 291], 'confirm': [46, 292, 2344], 'here': [47, 293], 'that': [48, 219, 230, 294, 465, 476, 550, 623, 869, 900, 1064, 1416, 1550, 1729, 1795, 1839, 1857, 1870, 1878, 1888, 1925, 1938, 1946, 2055, 2346, 2477, 2523, 2773, 3365, 3512, 3628, 4019], 'catalytic': [51, 297, 2297], 'cycle': [52, 298], 'involves': [53, 299], 'reaction': [56, 302, 604, 1212, 1404, 1680, 3221, 3260, 3319, 3388, 3416, 3826, 3857, 4087, 4116, 4133, 4181, 4221, 4254], 'intermediate': [57, 303, 605, 1405, 2350, 3261, 3389, 4238], 'which': [59, 88, 126, 184, 305, 334, 372, 430, 1057, 1682, 2291, 3687], 'histidine': [61, 307, 1233], 'residue': [62, 308, 1234, 1260], 'connected': [64, 310, 3060], 'to': [65, 91, 143, 165, 234, 311, 337, 389, 411, 480, 1217, 1243, 1268, 1272, 1283, 1408, 1456, 1461, 1539, 1547, 1736, 1891, 1913, 1919, 1950, 1958, 2021, 2174, 2186, 2193, 2237, 2269, 2437, 2447, 2465, 2677, 2788, 2797, 2911, 2943, 2952, 2972, 3000, 3023, 3079, 3090, 3150, 3185, 3294, 3311, 3357, 3424, 3507, 3602, 3692, 3791, 3846, 4152, 4230, 4352, 4439, 4466, 4474], '3′-phosphate': [68, 314, 566, 1441, 4097], 'through': [69, 315], 'phosphoamide': [71, 317, 1281, 1923, 2354, 2440], 'linkage.': [72, 318, 3337], 'Most': [73, 319, 1531, 2012, 2430], 'surprisingly,': [74, 320, 2431], 'can': [77, 103, 221, 323, 349, 467, 1940, 1964], 'be': [78, 92, 104, 324, 338, 350, 873, 1409, 1540, 1801, 2022], 'hydrolyzed': [79, 325], 'by': [80, 119, 326, 365, 1060, 1412, 1419, 1803, 1813, 2516, 2826, 2930, 3061, 3115, 3193, 3419, 3662, 4212, 4242, 4397, 4410], 'Tdp1,': [81, 97, 125, 327, 343, 371, 836, 1116, 1853], 'unlike': [83, 329], 'complex,': [87, 333, 3797], 'requires': [89, 335], 'modification': [90, 336], 'an': [93, 206, 339, 452, 544, 1674, 1885, 2014, 2449, 2946, 3094, 3748, 4287], 'efficient': [94, 340], 'substrate': [95, 341, 2026, 2719, 3149, 3192, 3209, 3252, 3802, 4154, 4473], 'for': [96, 342, 2027, 2503, 2560, 2782, 2855, 2921, 3291, 3329, 3412, 3504, 3526, 3589, 3657, 3817, 3833, 3952, 4029, 4070, 4204, 4281, 4339, 4354], 'native': [99, 345], 'form': [100, 123, 346, 369, 1732, 2509, 2768, 4371], 'removed': [105, 189, 351, 435], 'from': [106, 149, 190, 210, 241, 352, 395, 436, 456, 487, 547, 718, 885, 1421, 1894, 2453], 'DNA.': [108, 212, 354, 458, 616, 887, 1896, 1955], 'The': [109, 137, 355, 383, 1092, 1868, 1897, 2554, 2612, 2892, 2938, 2994, 3002, 3010, 3050, 3070, 3081, 3338, 3415, 3852, 3934], 'spinocerebellar': [110, 158, 356, 404, 1467], 'ataxia': [111, 159, 357, 405, 512, 535, 1468], 'with': [112, 160, 215, 358, 406, 461, 513, 536, 1439, 1469, 1542, 1687, 1928, 2667, 2764, 2815, 2832, 2866, 2885, 2897, 2981, 3122, 3212, 3306, 3322, 3343, 3349, 3397, 3635, 3722, 3747, 3806, 3914, 3926, 3958, 4051, 4074, 4095, 4111, 4119, 4136, 4189, 4225, 4258, 4286, 4335, 4455], 'axonal': [113, 161, 359, 407, 514, 537, 1470], 'neuropathy': [114, 360, 1471], 'neurodegenerative': [115, 361, 1465], 'disease': [116, 362], 'caused': [118, 364, 1802], 'H493R': [121, 367, 1641, 1851, 2507, 2837, 3402, 4194, 4235, 4324, 4330], 'mutant': [122, 147, 368, 393, 1552, 1731, 1745, 1852, 1859, 2508, 2563, 3879], 'shows': [127, 373], 'reduced': [128, 374, 1643], 'enzymatic': [129, 375, 545, 1638, 1808, 1886], 'activity': [130, 167, 182, 376, 413, 428, 546, 1639, 1887, 1903, 2177, 4003, 4013, 4102], 'accumulates': [132, 378], 'Tdp1-DNA': [134, 380, 1679, 1817, 1823, 1860, 2349, 3245, 3387, 4008, 4106, 4127], 'intermediate.': [136, 382, 3247, 4361], 'ability': [138, 384], 'wild': [140, 386], 'remove': [144, 235, 390, 481, 1892, 1966, 2448], 'protein': [148, 394, 3694], 'likely': [152, 398, 1899], 'explains': [153, 399], 'recessive': [155, 401, 1876], 'nature': [156, 402, 3153], 'neuropathy.': [162, 408], 'addition': [164, 410, 1957, 2173, 2268, 2932, 3421, 4214, 4399], 'its': [166, 412, 2175, 2485], 'on': [168, 414, 761, 1103, 1203, 1249, 1845, 1921, 2178, 2361, 3065, 3703, 3768, 3784, 3970, 4004, 4015, 4091, 4104, 4165], 'phosphotyrosine': [169, 415, 2284], 'phosphohistidine': [171, 417], 'substrates,': [172, 418, 1106, 2273, 3870], 'also': [174, 199, 420, 445, 1812, 1863, 1965, 2445], 'possesses': [175, 421], 'limited': [177, 423], 'RNA': [180, 426, 3012], '3′-exonuclease': [181, 427], 'single': [186, 432, 1960], 'nucleoside': [187, 433], '3′-hydroxyl': [192, 438], 'substrate.': [196, 442], 'Furthermore,': [197, 443, 2458], 'removes': [200, 446], 'abasic': [203, 449, 3096], 'site': [204, 450, 1069, 2684, 2737, 3097], 'artificial': [207, 453], '3′-biotin': [208, 454], 'adduct': [209, 455, 2230], 'combination': [214, 460], 'earlier': [216, 462, 1548], 'data': [217, 228, 463, 474, 896, 2366], 'showing': [218, 464], 'use': [222, 468], '3′-phosphoglycolate': [223, 469, 2229], 'as': [224, 470, 543, 632, 1108, 1427, 2245, 2282, 2356, 2634, 2688, 3780, 3989, 4066, 4227, 4240, 4304, 4417], 'substrate,': [226, 472], 'these': [227, 473, 2270, 2870, 3868], 'suggest': [229, 475], 'may': [232, 478, 1799], 'function': [233, 479, 1809], 'variety': [237, 483, 1100, 1943], 'adducts': [240, 486], 'during': [243, 489, 1520], 'repair.': [245, 491, 1737], 'Tyrosyl-DNA': [492], '2The': [495, 518], 'abbreviations': [496, 519], 'used': [497, 520, 2460, 3184, 3328], 'are:Tdp1tyrosyl-DNA': [498, 521], 'phosphodiesterasetopo70N-terminal': [499, 522], 'truncation': [500, 523], 'human': [502, 525, 1447, 2511, 2671, 2770], 'I': [504, 527, 883, 2717, 2772, 3190, 3378, 4078], 'missing': [505, 528, 2775], 'first': [506, 529, 1226, 2530], '174': [507, 530, 2779], 'amino': [508, 531, 2780], 'acidsPNKT4': [509, 532], 'polynucleotide': [510, 533, 3124], 'kinaseSCAN1spinocerebellar': [511, 534], 'neuropathyDTTdithiothreitolPLDphospholipase': [515, 538], 'Dwtwild': [516, 539], 'was': [541, 2514, 2686, 2762, 2824, 2879, 2909, 2928, 2941, 2998, 3008, 3021, 3183, 3210, 3320, 3341, 3347, 3355, 3366, 3395, 3417, 3445, 3496, 3518, 3671, 3690, 3699, 3720, 3804, 3827, 3844, 3859, 3881, 3987, 4064, 4109, 4117, 4134, 4150, 4187, 4223, 4256, 4333, 4374, 4414, 4437, 4453], 'discovered': [542], 'Saccharomyces': [548], 'cerevisiae': [549], 'specifically': [551], 'O-4': [558], 'atom': [559, 1252, 1286], 'tyrosine': [562, 1259, 1435, 1961, 1971], '(1Yang': [567, 840, 2070], 'S.W.': [568, 841, 2071], 'Burgin': [569, 842, 1384, 2072, 2309, 2327], 'Jr.,': [570, 843, 1385, 2073, 2310, 2328, 2739], 'A.B.': [571, 844, 1386, 2074, 2311, 2329, 2740], 'Huizenga': [572, 845, 2075, 2741], 'B.N.': [573, 846, 2076, 2742], 'Robertson': [574, 847, 1034, 2077], 'C.A.': [575, 848, 1035, 2078], 'Yao': [576, 849, 2079], 'K.C.': [577, 850, 2080], 'Nash': [578, 851, 974, 1036, 2081, 2743], 'H.A.': [579, 852, 975, 1037, 2082, 2744], 'Proc.': [580, 853, 1077, 1125, 1319, 1978, 2083, 2373, 2539, 2597, 2643, 2697, 3478], 'Natl.': [581, 854, 1078, 1126, 1320, 1979, 2084, 2374, 2540, 2598, 2644, 2698, 3479], 'Acad.': [582, 855, 1079, 1127, 1321, 1980, 2085, 2375, 2541, 2599, 2645, 2699, 3480], 'Sci.': [583, 856, 1080, 1128, 1144, 1161, 1178, 1322, 1981, 2086, 2376, 2542, 2600, 2646, 2700, 3481], 'U.': [584, 857, 1081, 1129, 1323, 1982, 2087, 2377, 2543, 2601, 2647, 2701, 3482], 'S.': [585, 803, 858, 1082, 1130, 1324, 1983, 2088, 2378, 2544, 2602, 2648, 2702, 3454, 3483], 'A.': [586, 859, 1083, 1131, 1325, 1984, 1998, 2037, 2089, 2150, 2208, 2379, 2545, 2603, 2649, 2703, 3484], '1996;': [587, 642, 860, 1145, 1162, 1179, 2090, 2259], '93:': [588, 861, 2091], '11534-11539Crossref': [589, 862, 2092], 'PubMed': [590, 645, 660, 673, 686, 699, 729, 780, 795, 815, 863, 942, 965, 982, 1005, 1027, 1043, 1087, 1135, 1151, 1165, 1185, 1199, 1308, 1329, 1348, 1371, 1393, 1502, 1574, 1608, 1631, 1664, 1712, 1765, 1787, 1988, 2007, 2046, 2093, 2116, 2140, 2167, 2222, 2262, 2318, 2336, 2383, 2406, 2425, 2549, 2586, 2607, 2653, 2707, 2751, 3045, 3463, 3488], 'Scopus': [591, 646, 661, 674, 687, 700, 730, 781, 864, 943, 966, 983, 1006, 1028, 1044, 1088, 1136, 1152, 1166, 1186, 1309, 1330, 1349, 1372, 1394, 1503, 1575, 1609, 1632, 1665, 1713, 1766, 1788, 1989, 2008, 2047, 2094, 2117, 2141, 2168, 2223, 2263, 2319, 2337, 2384, 2407, 2426, 2550, 2587, 2608, 2654, 2708, 2752, 3046, 3464, 3489], '(326)': [592, 865, 2095], 'Google': [593, 648, 663, 676, 689, 702, 732, 783, 796, 816, 827, 866, 945, 968, 985, 1008, 1030, 1046, 1090, 1138, 1154, 1168, 1188, 1200, 1311, 1332, 1351, 1374, 1396, 1505, 1577, 1611, 1634, 1667, 1715, 1768, 1790, 1991, 2010, 2049, 2096, 2119, 2143, 2170, 2225, 2265, 2321, 2339, 2386, 2409, 2428, 2552, 2589, 2610, 2656, 2710, 2754, 3048, 3466, 3491], 'Scholar).': [594, 703, 733, 828, 1091, 1201, 1506, 1668, 1716, 2011, 2050, 2171, 2226, 2266, 2429, 2553, 2611, 2711, 3049], 'This': [595], 'typical': [600], 'produced': [606], 'when': [607, 2233], 'IB': [610, 618], 'cleaves': [612], 'one': [613, 1854], 'strand': [614, 715, 769, 2724, 2759], 'Type': [617], 'topoisomerases': [619], 'are': [620, 1840, 1947, 2235], 'ubiquitous': [621], 'enzymes': [622], 'perform': [624], 'essential': [625], 'functions': [626, 1102, 2483], 'key': [628], 'cellular': [629, 2482], 'processes': [630], 'replication,': [633], 'recombination,': [634], 'transcription': [636, 1831], '(2Wang': [637], 'J.C.': [638, 651], 'Annu.': [639, 667, 723], 'Rev.': [640, 653, 668, 724, 1192], 'Biochem.': [641, 669, 725, 1143, 1194, 3459], '65:': [643], '635-692Crossref': [644], '(2061)': [647], 'Scholar,': [649, 664, 677, 690, 784, 797, 817, 946, 969, 986, 1009, 1031, 1139, 1155, 1169, 1189, 1312, 1333, 1352, 1375, 1578, 1612, 1769, 1992, 2097, 2120, 2144, 2322, 2387, 2410, 2590, 3467], '3Wang': [650], 'Nat.': [652, 1497, 1603], 'Mol.': [654, 1343, 1388, 2161, 2313, 3040], 'Cell.': [655, 2162], 'Biol.': [656, 807, 932, 1344, 1362, 1389, 2107, 2130, 2163, 2212, 2314, 2397, 3041], '2002;': [657, 1196, 1345, 1499, 1605, 2004, 2043, 2214, 3460], '3:': [658, 980], '430-440Crossref': [659], '(1870)': [662], '4Champoux': [665], 'J.J.': [666, 694, 722, 773, 926, 973, 996, 1021, 1033, 1074, 1076, 1122, 1124, 1302, 1316, 1318, 1339, 1358, 1658, 1706, 1781, 1975, 1977, 2103, 2202, 2370, 2372, 2393, 2416, 2536, 2538, 2580, 2594, 2596, 2640, 2642, 2694, 2696, 3038], '2001;': [670, 726, 792, 1040, 1084, 1132, 1326, 1985, 2380, 2546, 2604, 2650, 2704], '70:': [671, 727], '369-413Crossref': [672, 728], '(2153)': [675, 731], '5Nitiss': [678], 'J.L.': [679], 'Biochim.': [680], 'Biophys.': [681], 'Acta.': [682], '1998;': [683, 3485], '1400:': [684], '63-81Crossref': [685], '(307)': [688], '6Leppard': [691], 'J.B.': [692, 1019, 1300, 1656, 1704, 1779, 2578], 'Champoux': [693, 772, 1020, 1075, 1123, 1301, 1317, 1338, 1357, 1657, 1705, 1780, 1976, 2102, 2371, 2392, 2415, 2537, 2579, 2595, 2641, 2695, 3037], 'Chromosoma.': [695], '2005;': [696, 962, 1024, 1305, 1571, 1628, 1661, 1709, 1762, 1784, 2583], '114:': [697], '75-85Crossref': [698], '(175)': [701, 1187], 'Following': [704], 'cleavage': [705, 735, 2176, 2723, 2736, 2791, 3299], 'relaxation,': [708], 'normally': [711], 'religates': [712], 'dissociates': [717], '(4Champoux': [721], 'After': [734, 3737], 'near': [736], 'certain': [737], 'lesions': [739, 911], 'or': [740, 743, 1111, 2062, 2240, 2244, 2985, 3571, 3618, 3634, 3645, 3727, 3787, 3876, 4034, 4145, 4263, 4325, 4406], 'modified': [741, 2064], 'nucleotides,': [742], 'presence': [746, 3197], 'anti-cancer': [749], 'drug': [750, 2242], 'camptothecin,': [751], 'religation': [752], 'blocked,': [754], 'becomes': [758], 'covalently': [759, 880, 1270, 1948, 2675, 3309], 'trapped': [760, 2880], 'cleaved': [767], '(7Porter': [770], 'S.E.': [771], 'Nucleic': [774, 1625, 2001, 2040, 2330, 2745], 'Acids': [775, 1626, 2002, 2041, 2331, 2746], 'Res.': [776, 791, 823, 1627, 2003, 2042, 2258, 2332, 2747], '1989;': [777], '17:': [778, 1180], '8521-8532Crossref': [779], '(133)': [782], '8Pourquier': [785], 'P.': [786, 2128], 'Pommier': [787, 1999, 2038], 'Y.': [788, 1486, 1592, 2000, 2039, 3473], 'Adv.': [789], 'Cancer': [790, 822, 999], '80:': [793], '189-216Crossref': [794], '9Hsiang': [798], 'Y.H.': [799, 819], 'Hertzberg': [800], 'R.': [801], 'Hecht': [802], 'Liu': [804, 820, 2123, 2159], 'L.F.': [805, 821, 1624, 2124, 2160, 2210, 2256], 'J.': [806, 931, 1017, 1023, 1173, 1298, 1304, 1342, 1387, 1478, 1584, 1654, 1660, 1702, 1708, 1777, 1783, 2129, 2211, 2312, 2419, 2576, 2582, 3039, 3456, 3458], 'Chem.': [808, 933, 1361, 2106, 2131, 2213, 2396, 2421], '1985;': [809], '260:': [810], '14873-14878Abstract': [811], 'Full': [812, 937, 939, 1148, 1182, 1366, 1368, 2111, 2113, 2135, 2137, 2217, 2219, 2401, 2403], 'Text': [813, 938, 940, 1149, 1183, 1367, 1369, 2112, 2114, 2136, 2138, 2218, 2220, 2402, 2404], 'PDF': [814, 941, 1150, 1184, 1370, 2115, 2139, 2221, 2405], '10Hsiang': [818], '1988;': [824], '48:': [825], '1722-1726PubMed': [826], 'At': [829, 888, 3511, 3898, 4041, 4389], 'time': [831, 3513, 3901, 3985, 4392], 'discovery': [834], 'Yang': [837, 2151], 'et': [838], 'al.': [839], 'Scholar)': [867, 1397], 'suggested': [868, 2054], 'enzyme': [871, 1553], 'might': [872, 2479], 'involved': [874, 903, 3367, 3433], 'removal': [877], 'molecules': [884], 'present,': [889], 'extensive': [890], 'genetic,': [891], 'biochemical,': [892], 'cell': [894], 'biological': [895], 'support': [897, 2474], 'view': [899], 'cells': [914, 1740, 1838, 2234], '(11Barthelmes': [915], 'H.U.': [916], 'Habermeyer': [917], 'M.': [918, 952, 1492, 1561, 1598, 1752, 3036], 'Christensen': [919], 'M.O.': [920], 'Mielke': [921], 'C.': [922, 971], 'Interthal': [923, 1336, 1355, 2100, 2390, 2413], 'H.': [924, 1011, 1072, 1120, 1292, 1314, 1337, 1356, 1474, 1580, 1618, 1648, 1696, 1771, 1973, 2101, 2148, 2368, 2391, 2414, 2534, 2570, 2592, 2638, 2692], 'Pouliot': [925, 972, 995, 1073, 1121, 1315, 1974, 2201, 2369, 2535, 2593, 2639, 2693], 'Boege': [927], 'F.': [928, 954, 1563, 1754], 'Marko': [929], 'D.': [930, 1484, 1590, 2126], '2004;': [934, 979, 1002, 1390, 2315, 2333, 2422, 3042], '279:': [935], '55618-55625Abstract': [936], '(110)': [944, 1350], '12El-Khamisy': [947], 'S.F.': [948, 1557, 1748], 'Saifi': [949, 1479, 1558, 1585, 1749], 'G.M.': [950, 1480, 1559, 1586, 1750], 'Weinfeld': [951, 1560, 1751], 'Johansson': [953, 1562, 1753], 'Helleday': [955, 1564, 1755], 'T.': [956, 990, 1565, 1614, 1756, 2204], 'Lupski': [957, 1495, 1566, 1601, 1619, 1757], 'J.R.': [958, 1496, 1567, 1602, 1620, 1758], 'Caldecott': [959, 1568, 1759], 'K.W.': [960, 1569, 1760], 'Nature.': [961, 1570, 1761], '434:': [963, 1572, 1763], '108-113Crossref': [964, 1573, 1764], '(350)': [967, 1576, 1767], '13Liu': [970], 'Repair': [977], '(Amst.).': [978], '593-601Crossref': [981], '(35)': [984], '14Nivens': [987], 'M.C.': [988, 1379, 2304, 2324], 'Felder': [989], 'Galloway': [991], 'A.H.': [992], 'Pena': [993], 'M.M.': [994], 'Spencer': [997], 'H.T.': [998], 'Chemother.': [1000], 'Pharmacol.': [1001, 1177, 1195], '53:': [1003], '107-115Crossref': [1004], '(47)': [1007], '15Interthal': [1010, 1770], 'Chen': [1012, 1293, 1649, 1697, 1772, 2571], 'H.J.': [1013, 1294, 1650, 1698, 1773, 2572], 'Kehl-Fie': [1014, 1295, 1651, 1699, 1774, 2573], 'T.E.': [1015, 1296, 1652, 1700, 1775, 2574], 'Zotzmann': [1016, 1297, 1653, 1701, 1776, 2575], 'Leppard': [1018, 1299, 1655, 1703, 1778, 2577], 'EMBO': [1022, 1303, 1659, 1707, 1782, 2581], '24:': [1025, 1306, 1662, 1710, 1785, 2584], '2224-2233Crossref': [1026, 1307, 1663, 1711, 1786, 2585], '(161)': [1029, 1310, 1666, 1714, 1789, 2588], '16Pouliot': [1032], 'Genes': [1038], 'Cells.': [1039], '6:': [1041], '677-687Crossref': [1042], '(135)': [1045], 'Scholar).Tdp1': [1047], 'member': [1050], 'phospholipase': [1053], 'D': [1054], '(PLD)': [1055], 'superfamily,': [1056], 'characterized': [1059], 'two': [1061, 1220], '"HKD"': [1062], 'motifs': [1063], 'provide': [1065], 'conserved': [1067, 1209], 'active': [1068, 2683], 'residues': [1070], '(17Interthal': [1071, 1119, 1972, 2367, 2533, 2637, 2691], '98:': [1085, 1133, 1327, 1986, 2381, 2547, 2605, 2651, 2705], '12009-12014Crossref': [1086, 1134, 1328, 1987, 2382, 2548, 2606, 2652, 2706], '(236)': [1089, 1137, 1331, 1990, 2385, 2551, 2609, 2655, 2709], 'diverse': [1093], 'members': [1094], 'family': [1097], 'fulfill': [1098], 'vastly': [1104], 'different': [1105], 'phospholipids,': [1109], 'DNA,': [1110, 3167], 'case': [1114], 'DNA-protein': [1117], '18Koonin': [1140], 'E.V.': [1141], 'Trends': [1142, 1176], '21:': [1146], '242-243Abstract': [1147], '(140)': [1153, 3465], '19Ponting': [1156], 'C.P.': [1157], 'Kerr': [1158], 'I.D.': [1159], 'Protein': [1160], '5:': [1163], '914-922Crossref': [1164], '(282)': [1167], '20Morris': [1170], 'A.J.': [1171], 'Engebrecht': [1172], 'Frohman': [1174], 'M.A.': [1175, 1482, 1588], '182-185Abstract': [1181], '21Exton': [1190], 'J.H.': [1191], 'Physiol.': [1193], '144:': [1197], '1-94Crossref': [1198], 'Based': [1202], 'structural': [1204, 2363], 'biochemical': [1206, 2365], 'data,': [1207], 'PLD': [1210], 'superfamily': [1211], 'mechanism': [1213, 2298], 'proposed': [1216, 1242, 2359], 'consist': [1218, 2944], 'consecutive': [1221], 'SN2': [1222], 'substitutions.': [1223], 'step': [1227], 'reaction,': [1231, 3842], 'N-terminal': [1237, 2778], 'HKD': [1238, 1424], 'motif': [1239, 1425], '(His-263)': [1240], 'carry': [1244, 1510], 'out': [1245, 1411, 3673, 3779, 4303, 4385, 4416], 'nucleophilic': [1247, 1289], 'attack': [1248], 'phosphorus': [1251], '3′-oxygen': [1264], '(see': [1265, 1398, 2887, 3202, 3710], 'Fig.': [1266, 1399, 2888, 3203, 3716], '2B)': [1267], 'become': [1269], 'bound': [1271, 2676, 3310], 'via': [1279, 2681, 3695], 'N-ϵ2': [1285], 'His-263': [1290], '(15Interthal': [1291, 1647, 1695, 2569], '17Interthal': [1313, 2591], '22Davies': [1334], 'D.R.': [1335, 1354, 2099, 2389, 2412], 'Hol': [1340, 1359, 2104, 2394, 2417], 'W.G.': [1341, 1360, 2105, 2395, 2418], '324:': [1346], '917-932Crossref': [1347], '23Davies': [1353, 2098, 2388], '2003;': [1363, 2108, 2164, 2398], '10:': [1364, 2109, 2399], '139-147Abstract': [1365, 2110, 2400], '(109)': [1373, 2118, 2408], '24Raymond': [1376], 'A.C.': [1377, 2302, 2326], 'Rideout': [1378, 2303], 'Staker': [1380, 2305], 'B.': [1381, 2306], 'Hjerrild': [1382, 2307], 'K.': [1383, 1622, 2308], '338:': [1391, 2316], '895-906Crossref': [1392, 2317], '(51)': [1395, 2320], '2C).': [1400], 'Hydrolysis': [1401], 'believed': [1407], 'carried': [1410, 3672, 3778, 4302, 4384, 4415], 'water': [1414], 'molecule': [1415, 2452], 'activated': [1418], 'His-493': [1420, 1455], 'C-terminal': [1423], 'acting': [1426], 'general': [1429], 'base.': [1430], 'catalysis': [1432], 'produces': [1433], 'free': [1434], 'end.Recently,': [1442], 'mutation': [1444, 1512, 1672, 1873], 'TDP1': [1448, 1515, 2526], 'gene,': [1449], 'resulting': [1450, 2939], 'change': [1453], 'arginine,': [1457], 'shown': [1460, 1685, 2185, 4156], 'cause': [1462], 'heritable': [1464], 'disorder': [1466], '(SCAN1)': [1472], '(25Takashima': [1473], 'Boerkoel': [1475, 1581], 'C.F.': [1476, 1582], 'John': [1477, 1583], 'Salih': [1481, 1587], 'Armstrong': [1483, 1589], 'Mao': [1485, 1591], 'Quiocho': [1487, 1593], 'F.A.': [1488, 1594], 'Roa': [1489, 1595], 'B.B.': [1490, 1596], 'Nakagawa': [1491, 1597], 'Stockton': [1493, 1599], 'D.W.': [1494, 1600], 'Genet.': [1498, 1604], '32:': [1500, 1606, 2334], '267-272Crossref': [1501, 1607], '(427)': [1504, 1610], 'Affected': [1507], 'individuals': [1508, 1883], 'who': [1509], 'both': [1514, 2362], 'alleles': [1516, 2564], 'generally': [1517], 'develop': [1518], 'symptoms': [1519], 'their': [1521], 'teenage': [1522], 'years,': [1523], 'whereas': [1524], 'heterozygous': [1526, 1866], 'carriers': [1527], 'remain': [1528], 'symptom': [1529], 'free.': [1530], 'interestingly,': [1532, 2013], 'SCAN1': [1534, 1739, 1797, 1872], 'condition': [1535], 'does': [1536], 'not': [1537, 1800, 1881, 2434], 'seem': [1538], 'associated': [1541], 'increased': [1543], 'cancer': [1544], 'susceptibility.': [1545], 'Contrary': [1546], 'predictions': [1549], 'inactive': [1555], '(12El-Khamisy': [1556, 1747], '25Takashima': [1579], '26Zhou': [1613], 'Lee': [1615], 'J.W.': [1616], 'Tatavarthi': [1617], 'Valerie': [1621], 'Povirk': [1623, 2209], '33:': [1629], '289-297Crossref': [1630], '(128)': [1633], 'Scholar),': [1635, 1791, 3492], 'overall': [1637], 'only': [1644, 2435], 'about': [1645], '25-fold': [1646], 'More': [1669], 'importantly,': [1670], 'causes': [1673], 'accumulation': [1675, 1815, 4356], 'intermediate,': [1681, 4128, 4182], 'we': [1683, 2343, 2459], 'have': [1684, 2278, 2480], 'decays': [1686], 'half-life': [1689], '13': [1691], 'min': [1692, 2784, 2857, 3293, 3506, 3659, 3819, 4031, 4283, 4341], 'vitro': [1694, 2031], 'Ironically,': [1717], 'H493R-DNA': [1721, 3258, 4308], 'complex': [1722, 1728, 2018, 2884, 3300, 3362], 'replaces': [1723], 'attempts': [1735], 'Because': [1738], 'contain': [1741], 'substantial': [1742], 'amounts': [1743], 'finding': [1793], 'suggests': [1794, 1877], 'phenotype': [1798], 'mere': [1805], 'reduction': [1806], 'alone': [1810, 3633], 'but': [1811, 1880, 2443, 2978, 3092], 'complexes.': [1818, 4010, 4107], 'These': [1819, 2472], 'persistent': [1820], 'long': [1821], 'lived': [1822], 'could': [1828], 'significantly': [1829], 'impact': [1830], 'terminally': [1834], 'differentiated': [1835], 'nondividing': [1836], 'neuronal': [1837], 'primarily': [1841], 'affected': [1842], 'SCAN1.Based': [1844], 'our': [1846], 'current': [1847], 'understanding': [1848], 'would': [1855], 'predict': [1856], 'must': [1862], 'occur': [1864], 'carriers.': [1867], 'fact': [1869], 'completely': [1875], 'heterozygous,': [1879], 'homozygous,': [1882], 'possess': [1884], 'able': [1890, 1918, 2436, 2446], 'most': [1898], 'source': [1900], 'wt': [1905, 3726, 3874, 4089, 4262], 'itself,': [1907], 'possibility': [1910, 2476], 'prompted': [1911], 'us': [1912], 'test': [1914], 'whether': [1915], 'act': [1920], 'connects': [1926], 'intermediate.Previous': [1934], 'work': [1935], 'established': [1937], 'hydrolyze': [1941], 'groups': [1945], 'linked': [1949, 2951, 3691], 'moiety,': [1962], 'short': [1967], 'peptides': [1968], 'containing': [1969, 3707, 4368], '27Debethune': [1993], 'L.': [1994, 2033], 'Kohlhagen': [1995, 2034], 'G.': [1996, 2035], 'Grandas': [1997, 2036], '30:': [2005, 2044], '1198-1204Crossref': [2006, 2045], '(103)': [2009, 2048], 'intact': [2015, 2450], 'proven': [2020], 'very': [2024], 'poor': [2025], 'yeast': [2028], '(27Debethune': [2032], 'It': [2051], 'proteolytically': [2060], 'degraded': [2061], 'otherwise': [2063], 'before': [2065, 4038, 4056, 4138, 4284], 'action': [2067], 'vivo': [2069], '28Desai': [2121], 'S.D.': [2122, 2146], 'Vazquez-Abad': [2125], "D'Arpa": [2127], '1997;': [2132], '272:': [2133], '24159-24164Abstract': [2134], '(226)': [2142], '29Desai': [2145], 'Zhang': [2147, 3035], 'Rodriguez-Bauman': [2149], 'J.M.': [2152], 'Wu': [2153], 'X.': [2154], 'Gounder': [2155], 'M.K.': [2156], 'Rubin': [2157], 'E.H.': [2158], '23:': [2165, 2749], '2341-2350Crossref': [2166], '(122)': [2169], '3′-phosphotyrosyl': [2180], 'linkage,': [2181], 'cleave': [2187, 2438], 'linking': [2191], 'glycolate': [2192], '(30Inamdar': [2199], 'K.V.': [2200], 'Zhou': [2203], 'Lees-Miller': [2205], 'S.P.': [2206], 'Rasouli-Nia': [2207], '277:': [2215], '27162-27168Abstract': [2216], '(165)': [2224], 'Such': [2227], 'formed': [2232], 'exposed': [2236], 'ionizing': [2238], 'radiation': [2239], 'bleomycin': [2243], 'result': [2247], 'naturally': [2249, 2271], 'occurring': [2250, 2272], 'endogenous': [2251], 'reactive': [2252], 'oxygen': [2253], 'species': [2254], '(31Povirk': [2255], 'Mutat.': [2257], '355:': [2260], '71-89Crossref': [2261], '(333)': [2264], 'several': [2274, 2461], 'synthetic': [2275], 'substrates': [2277, 2464, 3118], 'identified': [2280], 'analogs,': [2285], '3′-(4-nitro-phenyl)': [2286], 'phosphate,': [2287], '(4-methyl-phenyl)-phosphate,': [2288], '4-methylumbelliferone,': [2290], 'proved': [2292], 'helpful': [2293], 'dissecting': [2295], '(24Raymond': [2301], '32Rideout': [2323], 'Raymond': [2325], '4657-4664Crossref': [2335], '(24)': [2338], 'Scholar).In': [2340], 'study,': [2342], 'chemically': [2345], 'indeed': [2352], 'had': [2357], 'based': [2360], '33Davies': [2411], 'Med.': [2420], '47:': [2423], '829-837Crossref': [2424], '(43)': [2427], 'efficiently': [2442], 'vitro.': [2457, 2471], 'additional': [2462, 2481, 3095], 'demonstrate': [2466], 'novel': [2467], 'activities': [2469], 'results': [2473], 'besides': [2484], 'role': [2486], 'lesions.EXPERIMENTAL': [2493], 'PROCEDURESMutagenesis,': [2494], 'Expression,': [2495], 'Purification': [2497], 'Proteins—The': [2500], 'expression': [2501, 2558], 'plasmid': [2502, 2520], 'N-terminally': [2505, 2613, 4327], 'truncated': [2506, 4328], 'Tdp1(Δ(1-148)': [2512], 'H493R)': [2513], 'generated': [2515, 2825], 'site-directed': [2517], 'mutagenesis': [2518], 'using': [2519, 3136, 3674], 'pHN1894S': [2521], 'Δ(1-148)': [2522, 2621, 4329], 'contains': [2524, 3093, 4082], 'gene': [2527], 'lacking': [2528], '444': [2531], 'bp': [2532], 'generation': [2555], 'plasmids': [2559], 'other': [2562, 3339], 'described': [2567, 2635, 2689, 3781, 4228, 4305, 4418], 'previously': [2568, 2636, 2690], 'His-tagged': [2614], 'recombinant': [2615], 'proteins': [2617], '(wt,': [2618], 'Δ(1-148),': [2619], 'H493R,': [2620, 2622, 4113], 'H263A)': [2624], 'were': [2625, 2864, 2894, 2919, 2970, 3113, 3119, 3134, 3174, 3553, 3584, 3600, 3622, 3629, 3651, 3655, 3666, 3745, 3777, 3907, 3912, 3920, 3937, 3956, 3968, 4020, 4046, 4163, 4210, 4276, 4301, 4383, 4395], 'expressed': [2626], 'Escherichia': [2628], 'coli': [2629], 'BL21': [2630], '(DE3)': [2631], 'purified': [2633], 'Scholar).Preparation': [2657], 'Substrates—Substrate': [2660], '20-TOPOpep,': [2661, 2977, 4092], '20-mer': [2663, 2800, 2812, 2958, 2963, 3051, 3072, 3082, 4072, 4094, 4452], 'oligonucleotide': [2665, 2801, 2814, 2974, 2996, 3005, 3013, 3027, 3053, 3073, 3084, 3305, 4443], '(5′-GTAGAGGATCTAAAAGACTT-3′)': [2666], 'small': [2669], 'trypsin-resistant': [2670], 'I-derived': [2673, 2821], 'peptide': [2674, 2950, 3379], 'tyrosine,': [2685], 'prepared': [2687], 'Briefly,': [2712], 'duplex': [2714], '30-mer': [2715], 'suicide': [2718, 2790, 3191, 3801], 'consisting': [2720, 3301], 'HEI-9': [2725, 3107], '(5′-GTAGAGGATCTAAAAGACTT*TGAAAAATTT,': [2726], 'where': [2727], '*': [2728], 'indicates': [2729], 'a5′-bridging': [2730], 'phosphorothiolate': [2731], 'at': [2733, 2785, 2858, 2924, 3100, 3288, 3409, 3501, 3586, 3741, 3820, 3830, 3895, 3949, 3983, 4026, 4201, 4278, 4342], '(34Burgin': [2738], '1995;': [2748], '2973-2979Crossref': [2750], '(77)': [2753], 'Scholar))': [2755], 'complementary': [2758, 2999], 'HEI-10': [2760], '(5′-AAATTTTTCAAAGTCTTTTAGATCCTCTAC)': [2761], 'incubated': [2763, 2920, 3211, 3396, 3497, 3585, 3630, 3721, 3805, 3829, 3894, 3948, 4025, 4110, 4188, 4257, 4277, 4334, 4454], 'topo70': [2765, 2796, 3216, 3810], '(a': [2766], '70-kDa': [2767], 'nonessential': [2777], 'acids)': [2781], '15': [2783], '37': [2786, 2859, 2925, 3410, 3587, 3742, 3821, 3896, 3950, 4202, 4279, 4343], '°C': [2787, 3411, 3503, 3588, 3832, 3951, 4028, 4203, 4280, 4344], 'allow': [2789, 4353, 4467], 'binding': [2794, 4469], 'remaining': [2799], '(20-Topo).': [2802], 'Subsequent': [2803, 4403], 'trypsin': [2804, 2908, 3346, 3946, 4054, 4137, 4226, 4407], 'treatment': [2805], 'gel': [2807, 3527, 4058, 4247, 4295, 4412], 'purification': [2808], 'resulted': [2809], 'single-stranded': [2813, 2964], 'predicted': [2817, 2942], '7-amino': [2818], 'acid': [2819, 2948, 3157, 3241, 3437, 3450], 'peptide.Substrate': [2822], '20-TDPpep': [2823, 2940, 3709, 4243], 'incubating': [2827], '0.05': [2828, 3391, 4183, 4446], 'pmol': [2829, 3206, 3392, 3799, 3864, 4184, 4447], '20-TOPOpep': [2831, 4063, 4108, 4186, 4336], '0.2': [2833, 3398, 3863, 4190], 'μg': [2834, 3214, 3399, 3808, 4191], 'Assay': [2839, 3407, 3731, 3815, 3891, 4199, 4267, 4346, 4366, 4464], 'Buffer': [2840, 3408, 3732, 3816, 3892, 4200, 4268, 4347, 4367, 4465], '(100': [2841], 'mm': [2842, 2845, 2850, 2853, 2900, 2905, 3224, 3227, 3232, 3530, 3577, 3647, 3758, 3924], 'KCl,': [2843, 3225], '10': [2844, 3226, 3505, 3590, 3735, 3834, 3923, 4030, 4271], 'Tris-HCl,': [2846, 2901, 3228, 3531, 3562, 3578, 3642, 3648], 'pH': [2847, 2902, 3229, 3532, 3563, 3579, 3643, 3649], '7.5,': [2848, 3230, 3564, 3580, 3644, 3650], '1': [2849, 2852, 2904, 2934, 3231, 3234, 3560, 3565, 3568, 3572, 3615, 3619, 3640, 3807], 'EDTA,': [2851, 3233, 3759], 'DTT)': [2854], '2': [2856, 2922, 3943, 3953, 4081, 4205, 4340], '°C,': [2860, 3743], 'reactions': [2863, 2893, 3583, 3627, 3744, 3911, 3967, 4049, 4162, 4209], 'stopped': [2865, 3321, 3418, 3746, 3913, 4050, 4118, 4211, 4396], '0.5%': [2867, 4216, 4401], 'SDS.': [2868, 4217, 4402], 'Under': [2869], 'conditions': [2871], 'more': [2872], 'than': [2873], '50%': [2874], 'labeled': [2877, 3494], '2A,': [2889, 3717], 'lane': [2890, 3718, 3998, 4061, 4158], '2).': [2891], 'diluted': [2895], '10-fold': [2896], 'TE': [2898], '(10': [2899], '8,': [2903], 'EDTA),': [2906], 'added': [2910, 3555, 3601, 3845, 3882, 4151, 4375, 4387], 'final': [2913, 3426, 3848, 3917], 'concentration': [2914, 3325, 3427, 3849, 4377], '1.5': [2916], 'μg/μl.': [2917], 'Samples': [2918, 3654, 3919, 3955], 'h': [2923], '°C.': [2926, 3822, 3897], 'Trypsin': [2927], 'inactivated': [2929, 3348], 'μg/μl': [2935, 3944], 'phenylmethylsulfonyl': [2936, 3350], 'fluoride.': [2937], '11-amino': [2947], 'Tdp1-derived': [2949], 'DNA.The': [2959], 'sequences': [2960], 'oligonucleotides': [2966, 3105, 3133, 3168], '20-Y': [2967, 3111, 4436], 'HEI-23': [2969, 3091], 'identical': [2971, 3076, 3087], 'portion': [2975, 4130], 'they': [2979], 'ended': [2980], '3′-tyrosyl': [2983], 'moiety': [2984, 3059], 'hydroxyl,': [2987], 'respectively,': [2988], 'place': [2990], 'peptide.': [2993, 4079], '3′-biotinylated': [2995, 4441], 'HEI-22': [2997], '20-Y.': [3001], '19-mer': [3003], 'HEI-24': [3006], '(5′-GTAGAGGATCTAAAAGACT-3′)': [3007], '3′-phosphorylated.': [3009], '29-mer': [3011], 'Md9': [3014], 'following': [3017], 'sequence': [3018, 3078, 3089], '5′-ACCCCACCUGUAGGUUUGGCAAGCUAGCU-3′': [3019], 'annealed': [3022, 4438], '48-mer': [3025], 'D49Δ8': [3028], '(5′-CTAGCTTGCCAAACCTACAGGTGGGGTCCCCCCTTTTCTGGAGACTAA-3′)': [3029], 'after': [3030], '5′': [3031], 'end-labeling': [3032], '(35Schultz': [3033], 'S.J.': [3034], '344:': [3043], '635-652Crossref': [3044], '(31)': [3047], 'HE-I-22': [3054], '(5′-AAGTCTTTTAGATCCTCTAC-3′)': [3055], 'biotin': [3058], '15-atom': [3063], 'spacer': [3064], '(BioTEG).': [3069], '3′-phosphorylated': [3071, 3132], 'HEI-27': [3074], 'HEI-22.': [3080], 'HEI-28': [3085], 'mimic': [3098], '(tetrahydrofuran)': [3099], 'end.All': [3103], 'except': [3106], '(Macromolecular': [3108], 'Resources)': [3109], '(Midland)': [3112], 'synthesized': [3114], 'OPERON.': [3116], 'All': [3117], '32P': [3120], '5′-end-labeled': [3121], 'T4': [3123], 'kinase': [3125], '(PNK)': [3126], '(New': [3127, 3180], 'England': [3128, 3181], 'Biolabs).': [3129], 'addition,': [3131], '32P5′-end-labeled': [3135], '3′-phosphatase': [3138], 'minus': [3139], 'PNK': [3140], '(MBI': [3141], 'Fermentas).Phosphoamino': [3142], 'Acid': [3143], 'Lability': [3144], 'Analyses—To': [3145], 'create': [3146], 'suitable': [3148], 'study': [3151], 'phosphoamino': [3156, 3240, 3436, 3449], 'CL12N': [3169], '(5′-GAAAAAAGAGTT-3′)': [3170], 'CP25N': [3172], '(5′-TAAAAATTTTTCTAACTCTTTTTTC-3′)': [3173], 'annealed,': [3175], 'Klenow': [3177], '(3′-5′': [3178], 'exo-)': [3179], 'Biolabs)': [3182], 'generate': [3186, 3295, 3358, 3793, 4177], '14-mer': [3188], 'fill-in': [3194], '[α-32P]dATP': [3199], 'ddGTP': [3201], '1A).': [3204], '50': [3205, 3723, 4259], '19': [3213], '200': [3218, 3576, 3646], 'μl': [3219, 3405, 3557, 3594, 3613, 3638, 3813, 3889, 3941, 3960, 4197, 4250], 'buffer': [3222, 3525, 3599, 3632], '(150': [3223], 'mmFIGURE': [3235], '1Chemical': [3236], 'characterization': [3237], 'A,': [3248, 4011], 'schematic': [3249], 'representation': [3250], 'preparation.': [3253], 'B,': [3254, 4100], 'SDS-PAGE': [3255, 3270, 4404], '(12-Tdp1)': [3262], 'samples': [3263, 3906, 4045, 4275, 4394], 'treated': [3264, 3272, 3551, 3700], 'under': [3265], 'indicated': [3267, 3900, 4043, 4370, 4391], 'conditions.': [3268], 'C,': [3269], '(12-Topo).': [3277, 3315], 'HA,': [3278], 'hydroxylamine.View': [3279], 'Large': [3280, 4169], 'Image': [3281, 3773, 4170], 'Figure': [3282, 4171], 'ViewerDownload': [3283, 4172], 'Hi-res': [3284, 4173], 'image': [3285, 3669, 4174, 4297], 'Download': [3286, 4175], '(PPT)DTT)': [3287], 'room': [3289], 'temperature': [3290], '45': [3292], '12-mer': [3304], 'Part': [3316, 4218], 'SDS': [3323, 3423, 3500, 3523, 3597, 3843, 3915, 4120], '(final': [3324, 3733, 4348, 4376], '1%)': [3326], 'parallel': [3331], 'analysis': [3332, 3528, 4405, 4413], 'part': [3340], 'digested': [3342, 4055, 4135, 4224], 'trypsin;': [3344], 'fluoride,': [3351], 'product': [3354, 3982, 4084], 'gel-purified': [3356], '12-TOPOpep.': [3359], 'phosphate': [3364, 3432], 'phosphotyrosyl': [3370, 3697], 'radioactively': [3381, 3446], 'labeled.': [3382], 'To': [3383, 3543, 3605, 3625, 3836, 3862, 4123], 'accumulate': [3384], '(12-Tdp1),': [3390], '12-TOPOpep': [3394], '25': [3404, 4196, 4350], '3': [3413, 3658], 'min.': [3414, 3591, 3835], '1%.': [3429], 'Again,': [3430], 'labeled.For': [3447], 'lability': [3451], 'experiment': [3452], '(36Klumpp': [3453], 'Krieglstein': [3455], 'Eur.': [3457], '269:': [3461], '1067-1071Crossref': [3462], '37Gottlin': [3468], 'E.B.': [3469], 'Rudolph': [3470], 'A.E.': [3471], 'Zhao': [3472], 'Matthews': [3474], 'H.R.': [3475], 'Dixon': [3476], 'J.E.': [3477], '95:': [3486], '9202-9207Crossref': [3487], '(129)': [3490], '12-Tdp1': [3495, 3552], '1%': [3499], '65': [3502, 3831, 4027], 'denature': [3508], 'protein.': [3510], '5-μl': [3515], 'control': [3516, 4381], 'sample': [3517, 3524, 3598, 3706], 'taken': [3519], 'boiled': [3521, 3656], '(62.5': [3529], '6.8,': [3533], '2%': [3534], 'SDS,': [3535, 4052], '10%': [3536, 3663], 'glycerol,': [3537], '5%': [3538], 'β-mercaptoethanol,': [3539], '0.001%': [3540], 'bromphenol': [3541, 3764], 'blue).': [3542], 'series': [3545], '10-μl': [3547], 'aliquots': [3548], 'then': [3554], '2.5': [3556, 3612, 3637, 3798], 'each': [3558, 3603, 3866, 3984], 'm': [3561, 3566, 3569, 3573, 3616, 3620, 3641], 'HCl,': [3567], 'NaOH,': [3570], 'hydroxylamine': [3574], 'Subsequently,': [3592], '4': [3593], '5×': [3596], 'reaction.': [3604], 'neutralize': [3606], 'acid-': [3608], 'base-treated': [3610], 'samples,': [3611], 'NaOH': [3617], 'HCl': [3621], 'added,': [3623, 3652], 'respectively.': [3624, 3653], 'hydroxylamine,': [3636], 'analyzed': [3661, 3767, 3969, 4164], 'SDS-PAGE.': [3664], 'Gels': [3665], 'dried,': [3667], 'retrieval': [3670, 3774], 'PhosphorImager': [3676], 'Image-Quant': [3678], 'software': [3679], '(Amersham': [3680], 'Biosciences).': [3681], 'As': [3682], 'control,': [3684], '12-Topo': [3685], 'identically.Tdp1': [3701], 'Activity': [3702, 3783], 'Phosphoamide': [3704], 'Bond—A': [3705], '>50%': [3708], '"Preparation': [3711], 'Substrates"': [3714], '2)': [3719, 4144], 'ng': [3724, 3872, 4260], 'H263A': [3728, 3878, 4146], 'volume': [3734, 3750, 3886, 4270, 4289, 4349, 4364], 'μl).': [3736], '30-min': [3739], 'incubation': [3740], 'equal': [3749, 4288, 4363], 'formamide': [3752, 3962, 4291], 'loading': [3753, 3963, 4139, 4292], 'dye': [3754], '(96%': [3755], 'formamide,': [3756], '20': [3757, 3812], '0.03%': [3760, 3763], 'xylene': [3761], 'cyanol,': [3762], 'blue)': [3765], '15%': [3770, 3972], 'sequencing': [3771, 3973, 4057, 4167, 4246, 4411], 'gel.': [3772, 3974], 'quantitation': [3776, 4300], 'above.Tdp1': [3782], 'Intact': [3785], 'Topo70': [3786], 'Covalently': [3789], 'Bound': [3790], 'DNA—To': [3792], 'topo70-DNA': [3795, 4016], 'HEI-9/HEI-10': [3803], '30': [3818, 4282], 'One-third': [3823], 'subsequently': [3828], 'second': [3838], 'third': [3839, 3854], '0.07%.': [3851], 'last': [3853], 'mixture': [3858, 4155, 4222, 4255], 'left': [3860], 'untreated.': [3861], 'three': [3869], '400': [3871], 'total': [3885, 3994], '60': [3888], 'points': [3902], '(Fig.': [3903], '3A),': [3904], '15-μl': [3905], 'taken,': [3908, 4047], '(0.5%': [3916], 'concentration).': [3918], 'precipitated': [3921], 'MgCl2': [3925], '5': [3927], 'volumes': [3928], 'ethanol': [3930], 'cold.': [3933], 'dry': [3935], 'pellets': [3936], 'resuspended': [3938], '8': [3940], 'h.': [3954], 'mixed': [3957], '12': [3959], 'dye,': [3964], 'For': [3975], 'purposes': [3976], 'quantitation,': [3978], 'amount': [3980, 4233], 'point': [3986], 'determined': [3988], 'percentage': [3991], 'radioactivity': [3995], 'gel.FIGURE': [4001], '3Tdp1': [4002], 'assays': [4014], 'either': [4021, 4322], 'untreated': [4022, 4253], '(lanes': [4023, 4032, 4036], '3-6),': [4024], '7-10),': [4033], 'SDS-denatured': [4035, 4105], '11-13)': [4037], 'addition.': [4040], 'times': [4044], 'analysis.': [4059, 4248], '1,': [4062, 4159], 'loaded': [4065], 'size': [4068], 'marker': [4069], 'tryptic': [4076], 'Lane': [4080], 'group': [4098], '(20-P).': [4099], 'assay': [4103], '(lane': [4121, 4140, 4143, 4147], '1).': [4122], 'visualize': [4124], '4).': [4141], 'Wt': [4142], '3)': [4148], 'gel.View': [4168], '(PPT)To': [4176], 'min,': [4206], 'above': [4229], 'determine': [4231], '(20-Tdp1)': [4239], 'reflected': [4241], '0.5': [4249], 'H263': [4264], '(total': [4269], 'μl),': [4272], 'stopping': [4285], 'dye.': [4293], 'Sequencing': [4294], 'analysis,': [4296], 'retrieval,': [4298], 'above.Fate': [4306], 'Intermediate': [4309], 'Presence': [4312], 'Various': [4314], 'Forms': [4315], 'Tdp1—A': [4317], '50-fold': [4318, 4478], 'molar': [4319], 'excess': [4320], 'full-length': [4323], '(0.1': [4331], 'μm)': [4332], '(2': [4337], 'nm)': [4338], 'μl)': [4351], 'enzyme-DNA': [4360], 'An': [4362], '0.25': [4378], 'μm).': [4379], 'Parallel': [4380], 'incubations': [4382], 'without': [4386], 'enzyme.': [4388], 'points,': [4393], 'digestion': [4408], 'followed': [4409], 'above.Determination': [4419], 'Half-life': [4422], 'DNA-H493R': [4425], 'Covalent': [4427], 'Complex': [4428], 'Absence': [4431], 'Free': [4433], 'Tdp1—5′': [4434], 'end-labeled': [4435], 'HEI-22,': [4444], 'fully': [4450], 'duplexed': [4451], 'streptavidin-conjugated': [4456], 'paramagnetic': [4457], 'beads': [4458], '(streptavidin': [4459], 'MagneSphere,': [4460], 'Promega)': [4461], 'equilibrated': [4462], 'complete': [4468], 'beads.': [4476], 'A': [4477], 'mol': [4479]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2082761467', 'counts_by_year': [{'year': 2024, 'cited_by_count': 7}, {'year': 2023, 'cited_by_count': 12}, {'year': 2022, 'cited_by_count': 11}, {'year': 2021, 'cited_by_count': 13}, {'year': 2020, 'cited_by_count': 11}, {'year': 2019, 'cited_by_count': 17}, {'year': 2018, 'cited_by_count': 9}, {'year': 2017, 'cited_by_count': 6}, {'year': 2016, 'cited_by_count': 7}, {'year': 2015, 'cited_by_count': 10}, {'year': 2014, 'cited_by_count': 15}, {'year': 2013, 'cited_by_count': 16}, {'year': 2012, 'cited_by_count': 15}], 'updated_date': '2024-12-18T16:59:26.025645', 'created_date': '2016-06-24'}