Title: Chromatographic and electrophoretic migration parameters in capillary electrochromatography
Abstract: ELECTROPHORESISVolume 23, Issue 9 p. 1211-1216 Research Article Chromatographic and electrophoretic migration parameters in capillary electrochromatography Anurag S. Rathore, Corresponding Author Anurag S. Rathore [email protected] Department of Chemical Engineering, Yale University, New Haven, CT, USAPharmacia Corporation, 700 Chesterfield Pkwy North, Chesterfield, MO 63017, USA (present address) Fax: +636-737-7281===Search for more papers by this authorCsaba Horváth, Csaba Horváth Department of Chemical Engineering, Yale University, New Haven, CT, USASearch for more papers by this author Anurag S. Rathore, Corresponding Author Anurag S. Rathore [email protected] Department of Chemical Engineering, Yale University, New Haven, CT, USAPharmacia Corporation, 700 Chesterfield Pkwy North, Chesterfield, MO 63017, USA (present address) Fax: +636-737-7281===Search for more papers by this authorCsaba Horváth, Csaba Horváth Department of Chemical Engineering, Yale University, New Haven, CT, USASearch for more papers by this author First published: 03 May 2002 https://doi.org/10.1002/1522-2683(200205)23:9<1211::AID-ELPS1211>3.0.CO;2-BCitations: 62AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Chromatographic retention factor, k′, as defined in high-performance liquid chromatography (HPLC) in terms of the migration times of the separand and the inert tracer, has limited applicability to capillary electrochromatography (CEC) when both chromatographic and electrophoretic processes determine the magnitude of the overall migration rates of the separands. This situation is unlike that in HPLC, where k′ serves as a useful peak locator for the various sample components, as well as, provides thermodynamic insights into the interactions between the components and the stationary phase. Most publications have borrowed the definition of k′ from HPLC and applied it on CEC. However, due to the dual separation mechanisms that are in action in CEC, the system is significantly complicated in comparison to that of HPLC. This paper discusses the impossibility of defining with a k′ which would have all the attributes that it has in regular chromatography. The interplay of the two separation mechanisms in determining the overall migration process in CEC is discussed and the various definitions of the electrochromatographic retention factor are presented. Citing Literature Volume23, Issue9Special Issue: Paper Symposium Capillary Electrochromatography and Electrokinetic Capillary ChromatographyNo. 9 May 2002Pages 1211-1216 RelatedInformation
Publication Year: 2002
Publication Date: 2002-05-01
Language: en
Type: review
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 69
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