Title: Differently Administered Colchicine Effects upon the Testis Cells of the Grasshopper, <i>Spathosternum prasiniferum</i>
Abstract:The effect of 0.1% colchicine administered by two different ways upon the testes cells of the grasshopper, Spathosternuyn prasiniferum has been studied.The colchicine treatment produced different kind...The effect of 0.1% colchicine administered by two different ways upon the testes cells of the grasshopper, Spathosternuyn prasiniferum has been studied.The colchicine treatment produced different kinds of changes in the division apparatus as well as on the morphology of the chromosomes.Qualitatively both the kinds of administrations produced similar types of abnormalities in the spermatogonial and spermatocyte cells. The different types of abnormalities observed have been described under four general headings:-1) mitotic changes-changes in the spermatogonial metaphase stage and in the testes sheath cells, 2) changes in the meiotic prophase stagescytoplasmic fusion to form multinucleate cells, failure of synapsis, reduction of chiasmata, and failure of spindle formation etc., 3) changes in the first and second spermatocyte metaphase stage-C-mitotic metaphase at metaphase II, breaking down of spindle, star or ball metaphase, grouping of chromosome disposition, sticky connection, multipolar spindle etc., 4) post-metaphase abnormalities-unoriented chromosome dispostion, asynchronous chromosomal division, lagging chromosomes, sticky chromosomes, pycnotic chromosomes etc.Quantitatively the food administered series did show more effect in the cells than that of the injection series. The types of changes observed commonly in the treated series were not observed in the control series. The quantitative analysis of some types of abnormalities such as C-mitosis, polyploid cell, polynucleate cell and cell with destroyed spindle revealed that the frequency of abnormalities increased with the prolongation of post-treatment fixation hour except for the frequency of C-mitotic cells in injection series and polyploid cells in the food administered series.The results have been discussed on the relative efficiency of different kinds of administration. Further, it has been revealed that the colchicine effect on the cells is enhanced with the extension of post-treatment fixation hour and the meiotic chromosomes seem to be more susceptible to colchicine effect.Read More