Title: On the tryptophan determination in lysozyme and its photoxidation products
Abstract: A comparative study was made of three techniques of tryptophan determination in lysozyme and its photoxidation products: mild acid hydrolysis (85–90°, 15 hr), pronase hydrolysis, and direct spectrophotometric determination. Satisfactory data have been obtained only after pronase hydrolysis. The technique for hydrolyzate treatment and its analysis have been worked out. This technique was used for tryptophan determination in three lysozyme photoxidation products. The mild acid hydrolysis has been found unsuitable owing to incomplete hydrolysis. The direct spectrophotometric determination has been modified as applied to lysozyme, and satisfactory results have been obtained. But this technique is unsuitable for tryptophan determination in photoxidized lysozymes as it does not permit differentiation of native from photoxidized tryptophan residues in protein.
Publication Year: 1968
Publication Date: 1968-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 7
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot