Title: The vasorelaxant effect and its mechanisms of sodium bisulfite as a sulfur dioxide donor
Abstract: To study the biological role of bisulfite on vascular contractility and its underlying cellular and molecular mechanisms, to explore whether bisulfite can be used as a sulfur dioxide (SO2) donor in the biological experiments, the vasorelaxant effects of sodium bisulfite and sodium sulfite on isolated rat thoracic aortic rings were compared; and the signal transduction pathways and the ion channels involved in the vascular effects of bisulfite were investigated. The results show that: (1) Sodium bisulfite relaxed rat thoracic aortic rings in a concentration-dependent manner (from 100 to 4000 μM); however, sodium sulfite at 500 and 1000 μM caused vasoconstriction, and only at higher concentrations (from 2000 to 4000 μM) it caused vasorelaxation in a concentration-dependent manner. (2) The vasorelaxation caused by the bisulfite at low concentrations (⩽500 μM) was endothelium-dependent, but at high concentrations (⩾1000 μM) it was endothelium-independent. (3) The vasorelaxation by the bisulfite at the low concentrations was partially mediated by the cGMP pathway and the vasorelaxation was related to big-conductance Ca2+-activated K+ (BKCa) channel, but not due to prostaglandin, protein kinase C (PKC) and cAMP pathways. (4) The vasorelaxation by the bisulfite at high concentrations was partially inhibited by tetraethylammonium (TEA) and glibenclamide, suggesting that the vasorelaxation was related to ATP-sensitive K+ channel (KATP) and L-type calcium-channel. These results led to the conclusion that bisulfite (HSO3-) might be a vasoactive factor and sodium bisulfite can be used as a SO2 donor for the study of SO2 biology.
Publication Year: 2012
Publication Date: 2012-07-02
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 88
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot