In Vivo Three-photon Calcium Imaging of Brain Activity from Layer 6 Neurons in Mouse Brain

Name: Work Video:
Duration: 3 min 30 s
Description: We demonstrate three-photon microscopy (3PM) at 1300-nm excitation for imaging neuronal activity as deep as layer-6 (L6) of adult mouse cortex using genetically-encoded calcium indicators. In vivo imaging of subcortical, GFP-labeled neurons is also demonstrated.
Dimitre G. Ouzounov; Nicholas G. Horton; Tianyu Wang; Danielle Feng; Nozomi Nishimura; Chris Xu
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