Title: Microbial Inulinases: Fermentation Process, Properties, and Applications
Abstract: The microbial enzyme, inulinase, hydrolyzes the plant polymer inulin into practically pure fructose. Several methods have been applied to prepare stable crude Kluyveromyces fragilis inulinase preparations: acetone drying of cells, P2O5, drying, and cell autolysis yielded preparations which were stable at 0°C for at least one month. The partial purification procedure for K. fragilis inulinase is based upon fixation and elution on DEAE-cellulose, the selective nonprecipitation of inulinase in saturated (NH0)2SO4,, and the reversible precipitation of inulinase with precooled acetone (–10°C). A semipurified inulinase preparation was obtained with about 55–65% recovery yield. Industrially applicable production schemes to produce Ultra High Fructose Glucose Syrups (UHFGS) from inulin have been proposed, with Jerusalem artichoke as the source of inulin. Recently, interest has been concentrated toward direct alcohol fermentation from inulin sources such as Jerusalem artichoke, using yeasts with inulinase activity. This research fits into the framework of research on new energy sources.
Publication Year: 1983
Publication Date: 1983-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 384
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