Title: The isolation and properties of pig plasma lipoproteins and partial characterisation of their apoproteins
Abstract: 1. Three major classes of lipoproteins (very low density, d < 1.006; low density, d 1.019–1.063; high density, d 1.063–1.21) were isolated by ultracentrifugation alone or by a combined method involving polyanion precipitation and ultracentrifugation. The physical, chemical and immunological properties of these lipoproteins (and of subfractions of very low and high density lipoproteins) were studied and compared with those of similar classes of lipoproteins in other species. 2. The apolipoproteins, obtained by complete delipidation of the lipoproteins, were partially characterised by gel filtration, polyacrylamide gel electrophoresis and by identification of their NH2-terminal residues. Very low density apolipoprotein separated into three components (two major and one minor) following gel filtration, in 8 M urea, through Sephadex G-150. Preliminary identification of the peaks indicated the presence of low density apolipoprotein in the first peak while the third peak consisted of four proteins with similar electrophoretic mobilities to the C group of proteins in human very low density lipoprotein. High density apolipoprotein separated into four fractions after chromatography through Sephadex G-200 (in 8 M urea) although heterogeneity was demonstrated in most fractions. 3. This investigation revealed many similarities between pig and human lipoproteins while preliminary investigations of the apolipoproteins further confirmed this.
Publication Year: 1973
Publication Date: 1973-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 64
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