Evaluation of a Multiplex PCR Assay (Bruce-ladder) for Molecular Typing of All <i>Brucella</i> Species, Including the Vaccine Strains

Name: Work Video:
Duration: 3 min 30 s
Description: An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.
Ignacio López‐Goñi; David García-Yoldi; C. M. Marín; María Jesús de Miguel; Pilar M. Muñoz; J.M. Blasco; Isabelle Jacques; Maggy Grayon; Axel Cloeckaert; Ana Cristina Ferreira; Regina Cardoso; Maria Inácia Corrêa de Sá; Karl Walravens; Dimpna C. Albert; Bruno Garin‐Bastuji
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