Title: atThin-layer chromatography for the quantitative separation of quinidine and quinidine metabolites from biological fluids and tissues
Abstract: A chromatographic method for the study of quinidine metabolism is described. Quinidine, dihydroquinidine and metabolites were separated by thin-layer chromatography on Kieselgel G with methanol-acetone (4:1) as the solvent system. The fluorescing bands were divided into four main fractions and extracted with ethanol-acetone (1:1) from the adsorbent layer of the plate. The fluorescence of each fraction was measured with and without addition of acetic acid and sulfuric acid. A standard curve was used to transform fluorescence into μg quinidine base. The recovery from the plates varied between 75 and 95 per cent. The results obtained by this method were highly reproducible. Amyl alcohol-benzene (1:1) was found optimal for the extraction of quinidine and its derivates from alkalized serum, urine and tissues. Removal of quenching substances and concentration of the quinidine bodies was achieved by transfer to sulfuric acid and second extraction with amyl alcohol-benzene. For further concentration, partial evaporation of the extraction solvent in a nitrogen atmosphere and at a temperature below 37° was used.
Publication Year: 1968
Publication Date: 1968-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 32
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