Title: The alteration of ribosomes for mRNA selection concerned with adenovirus growth in SV40-infected simian cells
Abstract: The SV40 helper function to support human adenovirus growth in simian cells previously infected with SV40 is mediated by an alteration of ribosomes to translate late adenovirus mRNA. Time sequence analysis of macromolecular synthesis in the doubly infected cells indicated that the amount of adenovirus capsid proteins synthesized was proportional to that of SV40 mRNA and SV40-induced cellular mRNA synthesized before synthesis was suppressed by adenovirus infection. These amounts were increased in parallel with the increase in the fraction of SV40 T antigen-positive cells in culture. An alteration of the preincubated S30 from the SV40-infected cells to respond to late adenovirus mRNA (24S) to synthesized hexon occurred nearly 15 hr after infection. This alteration was caused by the appearance of a new initiation factor for protein synthesis which modifies ribosomes and occurred irrespective of the induction of cellular DNA synthesis. A close correlation was observed between the amount of adenovirus capsid proteins synthesized in the doubly infected cells and the proportion of ribosomes altered to respond to late adenovirus mRNA. These results indicate that a certain early SV40 gene product acts to induced a new initiation factor for protein synthesis.
Publication Year: 1975
Publication Date: 1975-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 20
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