Title: Characterisation of antisera to recombinant IgA of the common brushtail possum (Trichosurus vulpecula)
Abstract: One of the limiting factors in understanding immune responses in marsupials is the scarcity of marsupial specific immunological reagents. This paper describes the characterisation of an antiserum raised against a recombinant protein of the constant region of the heavy chain of IgA (Cα) of the common brushtail possum (Trichosurus vulpecula). The availability of a marsupial specific anti-IgA provides a useful tool for the characterisation of mucosal immune responses in possums. Anti-Cα specifically detects IgA in possum serum and secretions using ELISAs, immuno-dot blots and Western blots without any cross-reactivity to IgG. The possum anti-Cα cross-reacts with IgA of koala (Phascolarctos cinereus), tammar wallaby (Macropus eugenii) and eastern grey kangaroo (Macropus giganteus), demonstrating the potential for use in other marsupials
Publication Year: 2002
Publication Date: 2002-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 4
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