Smad4 Protein Stability Is Regulated by Ubiquitin Ligase SCFβ-TrCP1

Name: Work Video:
Duration: 3 min 30 s
Mei Wan; Yi Tang; Ewan M. Tytler; Chongyuan Lu; Bingwen Jin; Selwyn M. Vickers; Lei Yang; Xingming Shi; Xu Cao
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'https://openalex.org/W2156417547', 'https://openalex.org/W2162067964', 'https://openalex.org/W2169344296', 'https://openalex.org/W2170634115', 'https://openalex.org/W2762075333'], 'related_works': ['https://openalex.org/W4396676821', 'https://openalex.org/W3195663446', 'https://openalex.org/W3110810129', 'https://openalex.org/W2102368462', 'https://openalex.org/W2054919679', 'https://openalex.org/W2040788846', 'https://openalex.org/W2032459623', 'https://openalex.org/W2016556888', 'https://openalex.org/W2007249806', 'https://openalex.org/W1970861248'], 'abstract_inverted_index': {'Smad4': [0, 38, 60, 71, 97, 137, 171, 173, 211, 233, 244, 270, 310, 344, 448, 463, 614, 900, 1004, 1017, 1029, 1272, 1377, 1416, 1428, 1452, 1545, 1590, 1613, 1629, 1632, 1638, 1677, 1693, 1695, 1723, 1763, 1787, 1797, 1830, 1850, 1873, 1895, 1908, 1942, 1954, 1982, 2011, 2024, 2050, 2069, 2076, 2105, 2247, 2249, 2259, 2271, 2299, 2358, 2383, 2476, 2675, 2686, 2699, 2732, 2756, 2817, 2823, 2927, 2968, 2980, 3005], 'is': [1, 17, 41, 103, 106, 116, 174, 190, 214, 276, 279, 289, 464, 477, 492, 637, 691, 932, 1014, 1374, 1535, 1585, 2062, 2077, 2087, 2245, 2483, 2491, 2506, 2514, 2670, 2679, 2684, 2705, 2796, 2834, 2846, 2991], 'a': [2, 47, 52, 175, 220, 225, 458, 503, 540, 552, 623, 646, 664, 701, 998, 1221, 1357, 1418, 1646, 2063, 2372, 2392, 2671, 2685, 2728, 2982], 'key': [3, 176, 668], 'intracellular': [4, 177, 414], 'mediator': [5, 178], 'for': [6, 55, 179, 228, 479, 549, 651, 1016, 1159, 1175, 1191, 1376, 1411, 2106, 2395, 2508, 2689, 2786, 3015, 3030, 3037, 3045, 3054, 3065], 'the': [7, 34, 56, 101, 120, 134, 146, 180, 207, 229, 274, 293, 307, 319, 373, 416, 435, 452, 465, 471, 508, 610, 619, 628, 656, 673, 679, 692, 723, 733, 935, 939, 946, 1044, 1099, 1129, 1137, 1205, 1364, 1438, 1459, 1467, 1532, 1603, 1624, 1650, 1662, 1668, 1674, 1701, 1714, 1748, 1754, 1758, 1766, 1780, 1801, 1808, 1836, 1853, 1897, 1905, 1910, 1947, 1976, 2005, 2047, 2074, 2078, 2084, 2173, 2182, 2185, 2189, 2195, 2222, 2227, 2240, 2275, 2361, 2376, 2386, 2404, 2492, 2497, 2529, 2707, 2723, 2736, 2753, 2761, 2779, 2792, 2801, 2804, 2837, 2841, 2848, 2860, 2960, 2972, 2999, 3002, 3018, 3038, 3046, 3066], 'transforming': [8, 181, 355], 'growth': [9, 14, 182, 187, 347, 356, 2213, 2234], 'factor-β': [10, 183, 348], '(TGF-β)': [11, 184, 349], 'superfamily': [12, 185], 'of': [13, 37, 59, 111, 124, 136, 186, 210, 232, 284, 297, 309, 381, 401, 457, 460, 488, 510, 630, 645, 661, 670, 683, 688, 934, 938, 948, 1039, 1050, 1140, 1168, 1194, 1252, 1349, 1437, 1470, 1502, 1568, 1626, 1649, 1661, 1667, 1676, 1703, 1716, 1756, 1761, 1783, 1803, 1818, 1855, 1894, 1899, 1907, 1912, 1938, 1949, 1978, 1995, 2007, 2020, 2049, 2082, 2123, 2145, 2175, 2184, 2194, 2205, 2224, 2243, 2278, 2388, 2742, 2755, 2765, 2781, 2803, 2836, 2974, 2979, 2996, 3004, 3020], 'factors': [15, 188, 395], 'and': [16, 75, 85, 105, 122, 155, 189, 248, 258, 278, 295, 328, 369, 377, 389, 408, 412, 449, 475, 512, 514, 546, 608, 653, 681, 700, 853, 882, 901, 974, 1019, 1030, 1037, 1047, 1054, 1083, 1101, 1114, 1120, 1157, 1162, 1183, 1204, 1243, 1273, 1356, 1379, 1395, 1476, 1517, 1526, 1596, 1689, 1745, 1786, 1792, 1835, 1861, 1883, 1931, 1966, 2016, 2071, 2086, 2127, 2162, 2169, 2191, 2262, 2325, 2384, 2401, 2420, 2463, 2496, 2511, 2698, 2733, 2759, 2763, 2818, 2824, 2853, 2865, 2928, 2990, 3058, 3062], 'also': [18, 191, 944, 1509, 1591, 2088, 2751, 2790, 2938], 'an': [19, 150, 156, 192, 323, 329, 1008, 1574, 1773, 2091, 2813], 'important': [20, 193], 'tumor': [21, 194], 'suppressor.': [22, 195], 'The': [23, 91, 196, 264, 1178, 1196, 1216, 1344, 1482, 1558, 1794, 1848, 1892, 1926, 2018, 2040, 2745], 'receptor-regulated': [24, 197, 359], 'Smad': [25, 198, 417, 489, 1440, 2124, 2525], '(R-Smad)': [26, 199], 'proteins': [27, 200, 418, 662, 1386, 1402, 1408, 1441, 1472, 1497], 'are': [28, 201, 396, 496, 1397, 2364, 2976, 3024], 'regulated': [29, 202, 497], 'by': [30, 96, 169, 203, 269, 342, 398, 470, 498, 618, 655, 696, 1128, 1563, 1719, 1845, 1985, 1989, 2028, 2200, 2216, 2221, 2297, 2475, 2701, 2722, 2809, 2821, 2828], 'ubiquitin-mediated': [31, 204, 499], 'degradation,': [32, 205, 1380, 2248, 2359], 'yet': [33, 206], 'precise': [35, 208, 486, 2362], 'control': [36, 209, 487, 1576], 'protein': [39, 57, 63, 212, 230, 236, 518, 704, 735, 885, 895, 1022, 1102, 1170, 1206, 1618, 1816, 1837, 1851, 2374, 2399, 2423, 2456, 2486, 2504, 2509, 2676, 2815, 2831, 2934], 'stability': [40, 213], 'unclear.': [42, 215], 'We': [43, 216, 887, 1006, 1264, 1620, 1709, 1919, 2285, 3023], 'have': [44, 217, 1812, 2353], 'identified': [45, 218], 'SCFβ-TrCP1,': [46, 219, 1757], 'ubiquitin': [48, 221, 674], '(E3)': [49, 222, 675], 'ligase,': [50, 223, 542], 'as': [51, 224, 385, 722, 732, 1096, 1363, 2090, 2219, 2812, 2950], 'critical': [53, 226, 478], 'determinant': [54, 227], 'degradation': [58, 123, 231, 296, 509, 550, 617, 654, 904, 947, 1038, 1138, 1276, 1838, 2070, 2387, 2402, 2424, 2764, 2777, 3003], 'protein.': [61, 138, 234, 311], 'F-box': [62, 235, 703, 734, 1021, 1359, 1385, 1401, 1407, 1617, 2373, 2455, 2485, 2500, 2830, 2933], 'β-TrCP1': [64, 115, 131, 237, 288, 304, 1023, 1394, 1431, 1445, 1503, 1511, 1686, 1718, 1739, 1767, 1785, 2457, 2527, 2690, 2819, 3048], 'in': [65, 73, 76, 167, 238, 246, 249, 340, 451, 551, 626, 1024, 1060, 1098, 1185, 1200, 1305, 1387, 1403, 1417, 1462, 1473, 1543, 1556, 1635, 1658, 1700, 1747, 1765, 1800, 1842, 1852, 1896, 1909, 1943, 1946, 1975, 1990, 2001, 2031, 2093, 2140, 2188, 2209, 2226, 2295, 2375, 2403, 2410, 2458, 2467, 2487, 2674, 2717, 2778, 2840, 2947, 2964, 2985, 2988, 3007], 'this': [66, 239, 992, 1025, 1583, 1822, 2481, 2682, 2718, 2766, 2787, 3008], 'E3': [67, 240, 505, 1000, 1009, 1313, 1346, 1389, 1857, 1876, 2065, 2378, 2396, 2412, 2489, 2805], 'ligase': [68, 241, 506, 690, 1001, 1010, 1373, 1390, 1858, 1877, 2066, 2379, 2397, 2413, 2490, 2807], 'interacts': [69, 242, 898, 1270, 1429, 1615, 2381], 'with': [70, 83, 89, 140, 243, 256, 262, 313, 447, 899, 1028, 1093, 1150, 1165, 1210, 1271, 1311, 1392, 1415, 1430, 1434, 1443, 1455, 1488, 1504, 1515, 1520, 1565, 1573, 1593, 1616, 1628, 1644, 1683, 1729, 1828, 1935, 2134, 2254, 2382, 2418, 2454, 2502, 2731, 2757, 2863, 2932, 2967, 2993], 'both': [72, 245, 678, 1496, 1784, 2160, 2695], 'yeast': [74, 247, 1419, 2460], 'mammalian': [77, 250, 1404, 1463, 2468], 'cells,': [78, 251, 1464, 1834, 2948], 'but': [79, 252, 1432, 1571, 2360, 2519], 'has': [80, 86, 253, 259, 1308, 1512, 1967, 2464, 2956], 'no': [81, 254, 1968], 'interaction': [82, 88, 93, 102, 255, 261, 266, 275, 1414, 1461, 1514, 1519, 1534, 1560, 1584, 1604, 2466, 2472, 2482, 2522, 2754], 'Smad2': [84, 257, 548, 1505, 1516, 1971], 'weak': [87, 260, 1518, 2465], 'Smad3.': [90, 263], 'β-TrCP1/Smad3': [92, 265, 1533, 1559, 2471], 'was': [94, 267, 1208, 1258, 1561, 1639, 1697, 1840, 1879, 1884, 1901, 1914, 1933, 1987, 2026, 2156, 2198, 2214, 2252, 2265, 2414, 2473], 'abolished': [95, 268, 1562, 2274, 2474], 'gene': [98, 271, 2038, 2095, 2260, 2272, 2477, 2969], 'silencing,': [99, 272, 2261, 2478, 2970], 'indicating': [100, 273, 1581, 2971], 'indirect': [104, 277, 2784], 'through': [107, 280, 2246], 'Smad4.': [108, 125, 281, 298, 1040, 1393, 1587, 1819, 2389], 'Ectopic': [109, 282, 2143], 'expression': [110, 135, 283, 308, 1675, 1696, 1740, 1759, 1781, 1798, 1955, 1963, 1972, 2144, 2204, 3032, 3049, 3056, 3069], 'SCF': [112, 147, 285, 320, 693, 727, 1312, 1345, 1371, 1388, 1651, 1669, 1804, 1809, 1824, 1856, 1875, 1900, 1913, 1927, 1951, 1996, 2135, 2147, 2161, 2230, 2244, 2255, 2279, 2377, 2488, 2772], 'complex': [113, 148, 286, 321, 728, 1011, 1026, 1372, 1652, 1670, 1805, 1810, 1928, 1952, 2730, 2748, 2773, 2808, 2843], 'containing': [114, 287], 'sufficient': [117, 290], 'to': [118, 291, 393, 454, 604, 648, 996, 1122, 1247, 1480, 1711, 1753, 1814, 2257, 2356, 2416, 3026], 'induce': [119, 292, 2258, 2421], 'ubiquitination': [121, 294, 671, 1018, 1036, 1251, 1378, 1983, 1992, 2006, 2025, 2048, 2400, 2510, 2700, 2762], 'Furthermore,': [126, 299, 1588, 2470], 'small': [127, 300, 366, 1549], 'interfering': [128, 301, 367, 1550], 'RNA-triggered': [129, 302], 'endogenous': [130, 303, 1544, 1589, 1594, 1631, 1717, 1738, 1762], 'suppression': [132, 305, 1715], 'increases': [133, 306, 2694], 'Consistent': [139, 312], 'these': [141, 314, 1471, 1609, 2056, 2288], 'results,': [142, 315], 'cells': [143, 316, 1033, 1090, 1142, 1179, 1197, 1475, 1547, 1643, 1726, 1769, 1844, 1945, 2003, 2225, 2966], 'that': [144, 317, 494, 729, 890, 943, 1002, 1013, 1268, 1427, 1451, 1485, 1582, 1612, 1807, 2043, 2060, 2067, 2239, 2270, 2290, 2370, 2480, 2517, 2681, 2706, 2771, 2798, 2859, 2940], 'overexpress': [145, 318], 'display': [149, 322, 1034], 'inhibited': [151, 324, 2158, 2231], 'TGF-β-dependent': [152, 325, 553], 'transcriptional': [153, 326, 480, 1045, 2112, 2152], 'activity': [154, 327, 1046, 2155, 2868], 'impaired': [157, 330], 'cell': [158, 331, 1234, 2032, 2186, 2196, 2212, 2233, 2282, 2951, 2961], 'cycle': [159, 332, 2197, 2283, 2952, 2962], 'arrest': [160, 333], 'function.': [161, 334], 'Thus,': [162, 335, 462, 2923, 2998], 'SCFβ-TrCP1': [163, 336, 1012, 1627, 1923, 1986, 2061, 2108, 2176, 2291, 2411, 2758, 2806, 2941, 2955, 2975], 'abrogates': [164, 337, 2292], 'TGF-β': [165, 338, 404, 473, 483, 1051, 1599, 1979, 2021, 2030, 2044, 2072, 2115, 2178, 2293, 2405, 2692, 2711, 2724, 2986], 'function': [166, 339, 1049, 2180, 2294, 2845], 'vivo': [168, 341, 1991, 2296], 'decreasing': [170, 343, 2298], 'stability.': [172, 345, 2300], 'Transforming': [346], '1The': [350], 'abbreviations': [351], 'used': [352], 'are:': [353], 'TGF-β,': [354, 2782], 'factor-β;': [357], 'R-Smad,': [358], 'Smad;': [360], 'E3,': [361], 'ubiquitin-protein': [362], 'isopeptide': [363], 'ligase;': [364], 'siRNA,': [365], 'RNA.': [368], 'related': [370], 'polypeptides': [371], 'constitute': [372], 'largest': [374], 'cytokine': [375], 'family': [376], 'regulate': [378, 455], 'many': [379, 631], 'aspects': [380], 'cellular': [382, 1048], 'processes': [383], 'such': [384, 2949], 'proliferation,': [386], 'differentiation,': [387], 'adhesion,': [388], 'apoptosis.': [390], 'Signaling': [391], 'responses': [392], 'TGF-β-like': [394], 'mediated': [397, 1984], 'two': [399, 1398], 'types': [400], 'transmembrane': [402], 'receptors,': [403], 'type': [405, 409], 'I': [406], 'receptor': [407], 'II': [410], 'receptor,': [411], 'their': [413, 884, 1412, 1478], 'substrates,': [415], '(1Massague': [419], 'J.': [420, 561, 797, 965, 981, 2309, 2587, 2884, 2910, 2914, 3042], 'Annu.': [421, 707], 'Rev.': [422, 708], 'Biochem.': [423], '1998;': [424, 761, 2554], '67:': [425], '753-791Crossref': [426], 'PubMed': [427, 534, 572, 597, 715, 764, 787, 820, 848, 877, 926, 969, 986, 1298, 1339, 2320, 2348, 2445, 2557, 2579, 2610, 2637, 2664, 2888, 2918], 'Scopus': [428, 535, 573, 598, 716, 765, 788, 821, 849, 878, 927, 970, 987, 1299, 1340, 2321, 2349, 2446, 2558, 2580, 2611, 2638, 2665, 2889, 2919], '(3999)': [429], 'Google': [430, 537, 575, 600, 718, 767, 790, 823, 851, 880, 929, 972, 989, 1301, 1342, 2323, 2351, 2448, 2560, 2582, 2613, 2640, 2667, 2891, 2921], 'Scholar).': [431, 538, 601, 930, 990, 1302, 1343, 2449, 2668, 2922], 'Following': [432], 'ligand': [433], 'binding,': [434], 'activated': [436, 2708], 'receptors': [437], 'directly': [438], 'phosphorylate': [439, 2926], 'regulatory': [440, 632], 'Smads': [441, 495], '(R-Smads).': [442], 'Phosphorylated': [443], 'R-Smads': [444, 2085, 2709], 'form': [445], 'complexes': [446, 694], 'accumulate': [450], 'nucleus': [453, 2737], 'transcription': [456, 2741], 'variety': [459], 'genes.': [461, 2744], 'common': [466, 2080], 'signaling': [467, 484, 519, 2406], 'molecule': [468], 'shared': [469], 'entire': [472], 'superfamily,': [474], 'it': [476, 650, 2103, 2739], 'activation.': [481], 'Proper': [482], 'requires': [485, 663], 'functions.': [490], 'It': [491, 602, 2513, 2678, 2795], 'reported': [493, 2355], 'degradation.': [500, 615, 886, 1005, 1020, 2075, 2512, 2526], 'Specifically,': [501], 'Smurf1,': [502], 'Hect-domain-containing': [504], 'triggers': [507], 'Smad1': [511, 545], 'Smad5': [513], 'inhibits': [515, 2150], 'bone': [516], 'morphogenetic': [517], '(2Zhu': [520], 'H.': [521, 807, 833, 2597, 2622], 'Kavsak': [522], 'P.': [523, 773, 775, 957, 2565, 2567, 2876, 2902], 'Abdollah': [524], 'S.': [525, 592, 749, 803, 912, 1284, 1316, 2334, 2542, 2593, 2894], 'Wrana': [526], 'J.L.': [527], 'Thomsen': [528], 'G.H.': [529], 'Nature.': [530, 760, 873, 982, 2553, 2660], '1999;': [531, 712, 784, 983, 2576], '400:': [532], '687-693Crossref': [533], '(693)': [536], 'Smurf2,': [539], 'Smurf1-related': [541], 'can': [543], 'target': [544, 649, 2743], 'sometimes': [547], 'manner': [554], '(3Lin': [555], 'X.': [556, 908, 916, 920, 955, 1280, 1288, 1292, 2330, 2338, 2342, 2874, 2906], 'Liang': [557], 'M.': [558, 745, 755, 858, 860, 870, 906, 1278, 2328, 2426, 2434, 2538, 2548, 2645, 2647, 2657, 2908], 'Feng': [559], 'X.-H.': [560], 'Biol.': [562, 711, 816, 844, 2310, 2440, 2606, 2633], 'Chem.': [563, 2311], '2000;': [564], '275:': [565], '36818-36822Abstract': [566], 'Full': [567, 569, 2315, 2317], 'Text': [568, 570, 2316, 2318], 'PDF': [571, 2319], '(408)': [574], 'Scholar,': [576, 2561, 2583, 2614, 2641, 2892], '4Zhang': [577], 'Y.': [578, 759, 801, 811, 910, 979, 1282, 2332, 2552, 2591, 2601], 'Chang': [579], 'C.': [580, 831, 961, 1326, 2620, 2880], 'Gehling': [581], 'D.J.': [582], 'Hemmati-Brivanlou': [583], 'A.': [584, 593, 741, 743, 805, 868, 1320, 1332, 2534, 2536, 2595, 2655], 'Derynck': [585], 'R.': [586, 839, 953, 2628, 2872, 2898, 2900, 2912], 'Proc.': [587], 'Natl.': [588], 'Acad.': [589], 'Sci.': [590], 'U.': [591], '2001;': [594, 845, 966, 1336, 2312, 2442, 2634, 2885], '98:': [595], '974-979Crossref': [596], '(428)': [599], 'remains': [603], 'be': [605], 'determined': [606, 1841], 'whether': [607, 1370, 1384, 1531, 1623, 1713, 1922], 'how': [609], 'ubiquitin-proteasome': [611, 620], 'pathway': [612, 621], 'regulates': [613, 1630, 2740, 2800], 'Protein': [616], 'plays': [622, 2981], 'vital': [624], 'role': [625, 2394, 2716, 2984], 'monitoring': [627], 'abundance': [629], 'proteins.': [633], 'A': [634, 667, 686, 2168], 'polyubiquitin': [635], 'chain': [636], 'built': [638], 'onto': [639], 'one': [640, 933, 2835], 'or': [641, 1446, 1506, 1598, 1655, 1657, 1685, 1732, 1941, 2148, 2820], 'multiple': [642], 'lysine': [643], 'residues': [644], 'substrate': [647, 684, 724, 1365, 2493, 2505, 2826], 'capture': [652], '26': [657], 'S': [658, 2192], 'proteasome.': [659], 'Ubiquitination': [660, 1229], 'multienzyme': [665], 'system.': [666], 'component': [669, 1648, 1666], 'pathways,': [672], 'ligases,': [676], 'controls': [677], 'specificity': [680], 'timing': [682], 'ubiquitination.': [685], 'class': [687], 'ubiquiting': [689], 'formed': [695], 'Skp1,': [697, 1355], 'cullin1,': [698], 'Roc1,': [699, 1354], 'variable': [702, 1358], '(5Deshaies': [705], 'R.J.': [706, 1334], 'Cell': [709], 'Dev.': [710, 783, 2575], '15:': [713], '435-467Crossref': [714], '(1083)': [717], 'Scholar),': [719, 768, 791, 824, 852], 'which': [720, 1361], 'serves': [721], 'recognition': [725, 1366, 2494, 2827], 'subunit.': [726, 1367], 'contains': [730], 'β-TrCP': [731], '(SCFβ-TrCP)': [736], 'recognizes': [737, 2528], 'phosphorylated': [738, 2530], 'IκB': [739], '(6Yaron': [740, 2533], 'Hatzubai': [742, 2535], 'Davis': [744, 2537], 'Lavon': [746, 2539], 'I.': [747, 827, 2540, 2616], 'Amit': [748, 2541], 'Manning': [750, 2543], 'A.M.': [751, 2544], 'Andersen': [752, 2545], 'J.S.': [753, 2546], 'Mann': [754, 2547], 'Mercurio': [756, 2549], 'F.': [757, 841, 2550, 2630], 'Ben-Neriah': [758, 810, 2551, 2600], '396:': [762, 2555], '590-594Crossref': [763, 2556], '(572)': [766, 2559], 'β-catenin': [769], '(7Winston': [770], 'J.T.': [771, 2563], 'Strack': [772, 2564], 'Beer-Romero': [774, 2566], 'Chu': [776, 2568], 'C.Y.': [777, 2569], 'Elledge': [778, 2570], 'S.J.': [779, 2571], 'Harper': [780, 2572, 3044], 'J.W.': [781, 2573], 'Genes': [782, 2574], '13:': [785, 2577], '270-283Crossref': [786, 2578], '(817)': [789, 2581], 'NF-κB': [792], 'p105': [793], '(8Lang': [794], 'V.': [795, 2585], 'Janzen': [796, 2586], 'Fischer': [798, 2588], 'G.Z.': [799, 2589], 'Soneji': [800, 2590], 'Beinke': [802, 2592], 'Salmeron': [804, 2594], 'Allen': [806, 2596], 'Hay': [808, 2598], 'R.T.': [809, 2599], 'Ley': [812, 2602], 'S.C.': [813, 2603], 'Mol.': [814, 842, 2439, 2604, 2631], 'Cell.': [815, 843, 2441, 2605, 2632], '2003;': [817, 874, 2607, 2661, 2915], '23:': [818, 2608], '402-413Crossref': [819, 2609], '(108)': [822, 2612], 'ATF4': [825], '(9Lassot': [826], 'Segeral': [828, 2617], 'E.': [829, 2618], 'Berlioz-Torrent': [830, 2619], 'Durand': [832, 2621], 'Groussin': [834, 2623], 'L.': [835, 856, 914, 1286, 2336, 2624, 2643], 'Hai': [836, 2625], 'T.': [837, 1324, 2428, 2430, 2432, 2626], 'Benarous': [838, 2627], 'Margottin-Goguet': [840, 2629], '21:': [846, 2635], '2192-2202Crossref': [847, 2636], '(206)': [850, 2639], 'Cdc25A': [854], '(10Busino': [855], 'Donzelli': [857, 2644], 'Chiesa': [859, 2646], 'Guardavaccaro': [861, 2648], 'D.': [862, 864, 951, 2304, 2649, 2651, 2870, 2904], 'Ganoth': [863, 2650], 'Dorrello': [865, 2652], 'N.V.': [866, 2653], 'Hershko': [867, 2654], 'Pagano': [869, 2656], 'Draetta': [871, 2658], 'G.F.': [872, 2659], '426:': [875, 2662], '87-91Crossref': [876, 2663], '(363)': [879, 2666], 'Scholar)': [881, 973, 2324, 2352], 'mediates': [883, 1003, 2068], 'previously': [888, 1266], 'demonstrated': [889, 1267, 1484, 2042, 2770, 3006], 'Jun': [891], 'activation': [892, 2721], 'domain': [893], 'binding': [894, 2125, 2498], '1': [896, 1076], '(Jab1)': [897], 'induces': [902, 945, 1274, 2385], 'its': [903, 1275, 2503, 2829, 2930], '(11Wan': [905, 1277, 2327], 'Cao': [907, 919, 1279, 1291, 2329, 2341], 'Wu': [909, 913, 1281, 1285, 2331, 2335], 'Bai': [911, 1283, 2333], 'Shi': [915, 1287, 2337, 3036], 'Wang': [917, 1289, 2339], 'N.': [918, 1290, 1330, 2340], 'EMBO': [921, 964, 1293, 2343, 2883, 2913], 'Rep.': [922, 1294, 2344], '2002;': [923, 1295, 2345], '3:': [924, 1296, 2346], '171-176Crossref': [925, 1297, 2347], '(140)': [928, 1300, 2350], 'Jab1': [931, 1269, 1597, 2138, 2149, 2163, 2326, 2789, 2799, 2833], 'eight': [936, 2838], 'subunits': [937, 1351, 2839], 'COP9': [940, 1306], 'signalosome': [941], '(CSN)': [942], 'p53': [949], '(12Bech-Otschir': [950, 2869], 'Kraft': [952, 2871], 'Huang': [954, 2873, 2905], 'Henklein': [956, 2875, 2901], 'Kapelari': [958, 2877], 'B.': [959, 2878], 'Pollmann': [960, 2879], 'Dubiel': [962, 2881], 'W.': [963, 2882], '20:': [967, 2886], '1630-1639Crossref': [968, 2887], '(328)': [971, 2890], 'p27': [975], '(13Tomoda': [976], 'K.': [977, 2436, 2438], 'Kubota': [978], 'Kato': [980], '398:': [984], '160-165Crossref': [985], '(551)': [988], 'In': [991, 1227, 1751, 1870], 'study,': [993], 'we': [994, 1381, 1465, 2368], 'sought': [995], 'identify': [997], 'Jab1-correlated': [999], 'found': [1007, 2415], 'responsible': [1015, 1375], 'associates': [1027, 1454], 'SCFβ-TrCP': [1031, 1042], 'overexpressing': [1032], 'increased': [1035, 1764, 1815], 'Consequently,': [1041], 'reduced': [1043, 1737], 'signaling.': [1052], 'Immunoprecipitation': [1053], 'Western': [1055, 1255], 'Blotting': [1056], 'Analysis—Cells': [1057], 'were': [1058, 1091, 1109, 1112, 1115, 1126, 1143, 1163, 1180, 1198, 1218, 1236, 1409, 1498, 1508, 1540, 1727, 1826, 2132, 2164], 'lysed': [1059, 1089, 1199], '150': [1061], 'mm': [1062, 1071, 1077], 'NaCl,': [1063], '1%': [1064], 'Triton': [1065], 'X-100,': [1066], '0.5%': [1067], 'deoxycholic': [1068], 'acid,': [1069], '50': [1070], 'Tris': [1072], 'buffer,': [1073, 1203], 'pH': [1074], '7.5,': [1075], 'phenylmethylsulfonyl': [1078], 'fluoride,': [1079], '10': [1080, 1084], 'μg/ml': [1081, 1085], 'aprotinin,': [1082], 'leupeptin.': [1086], 'For': [1087], 'immunoprecipitation,': [1088], 'incubated': [1092, 1144, 1184], 'different': [1094], 'antibodies': [1095], 'indicated': [1097], 'figures': [1100], 'G': [1103], 'plus': [1104], 'agarose': [1105], 'beads': [1106], '(Amersham': [1107, 1132], 'Biosciences)': [1108], 'added.': [1110], 'Immunocomplexes': [1111], 'washed': [1113, 1182], 'separated': [1116, 1219], 'on': [1117, 1220, 1970, 2022, 2177, 2280, 2959], 'SDS-polyacrylamide': [1118], 'gel': [1119, 1214, 1224], 'blotted': [1121], 'nitrocellulose.': [1123], 'All': [1124], 'blots': [1125], 'developed': [1127], 'ECL': [1130], 'technique': [1131], 'Biosciences).': [1133], 'Pulse-Chase': [1134], 'Assays—To': [1135], 'measure': [1136], 'rate': [1139, 1839], 'Smad4,': [1141, 1254, 1537], 'at': [1145, 1863, 1887, 2847], '48': [1146], 'h': [1147, 1865, 1889, 1916, 2036], 'after': [1148, 1232, 2037], 'transfection': [1149], "Dulbecco's": [1151, 1187], 'modified': [1152, 1188], "Eagle's": [1153, 1189], 'medium': [1154, 1190, 2034], 'without': [1155, 1874], 'methionine': [1156], 'cysteine': [1158], '30': [1160], 'min': [1161], 'pulse-labeled': [1164], '100': [1166], 'μCi': [1167], '[35S]methionine-cysteine': [1169], 'labeling': [1171], 'mix': [1172], 'per': [1173], 'ml': [1174], '40': [1176], 'min.': [1177], 'then': [1181, 1244, 1541, 1621, 1920], 'complete': [1186], 'various': [1192, 1659], 'periods': [1193], 'time.': [1195], 'radioimmunoprecipitation': [1201], 'assay': [1202, 1424], 'extract': [1207], 'immunoprecipitated': [1209, 1237], 'anti-FLAG': [1211], 'M2-agarose': [1212], 'affinity': [1213], '(Sigma).': [1215], 'precipitates': [1217], '10%': [1222], 'polyacrylamide': [1223], 'before': [1225], 'autoradiography.': [1226], 'Vivo': [1228], 'Assays—Forty': [1230], 'hours': [1231], 'transfection,': [1233], 'lysates': [1235], 'using': [1238, 1260, 1548], 'antibody': [1239], 'against': [1240], 'FLAG,': [1241], 'boiled,': [1242], 'reprecipitated': [1245], 'prior': [1246], 'immunoblotting.': [1248], 'To': [1249, 1368, 1457, 1528, 1820, 2171, 2236], 'detect': [1250], 'precipitated': [1253], 'blot': [1256], 'analysis': [1257], 'performed': [1259, 1542], 'anti-ubiquitin': [1261], '(Ubi)': [1262], 'antibody.': [1263], 'had': [1265], 'Jab1,': [1303], 'integrated': [1304], 'complex,': [1307, 2380], 'intense': [1309], 'association': [1310, 1625, 2697, 2931], 'ligases': [1314, 1347], '(14Lyapina': [1315], 'Cope': [1317], 'G.': [1318, 1322], 'Shevchenko': [1319, 1331], 'Serino': [1321], 'Tsuge': [1323], 'Zhou': [1325], 'Wolf': [1327], 'D.A.': [1328], 'Wei': [1329], 'Deshaies': [1333], 'Science.': [1335], '292:': [1337], '1382-1385Crossref': [1338], '(562)': [1341], 'consist': [1348], 'four': [1350], 'including': [1352], 'Cullin,': [1353], 'protein,': [1360], 'functions': [1362], 'determine': [1369, 1712], 'first': [1382, 1749], 'tested': [1383], 'interact': [1391, 2417, 2453], 'Skp2': [1396, 1447], 'well': [1399], 'characterized': [1400], 'cells.': [1405, 1636, 2142, 2469], 'Both': [1406], 'examined': [1410, 1622, 1921, 1988, 2027], 'possible': [1413, 2680, 2797], 'two-hybrid': [1420, 2461], 'assay.': [1421], 'Liquid': [1422], 'β-galactosidase': [1423], 'results': [1425, 1483, 1610, 2041], 'indicate': [1426, 1611, 2059, 2939], 'not': [1433, 1572, 1672, 1778, 2365, 2452, 2520], 'Skp2.': [1435], 'None': [1436], 'other': [1439], 'interacted': [1442], 'either': [1444, 1645, 1730, 2146, 2810], '(Fig.': [1448, 1491, 1522, 1579, 1605, 1678, 1741, 1788, 1866, 1917, 1956, 2012, 2051, 2166], '1A),': [1449], 'suggesting': [1450, 2479], 'specifically': [1453, 1614], 'SCFβ-TrCP1.': [1456, 2206, 2702], 'confirm': [1458, 2238], 'Smad4/β-TrCP1': [1460, 2696, 2793], 'overexpressed': [1466, 2165], 'epitope-tagged': [1468, 1936], 'versions': [1469], '293T': [1474, 1642, 1725, 1833, 1843, 1944, 2002], 'assessed': [1477], 'ability': [1479], 'coimmunoprecipitate.': [1481], 'FLAG-Smad4': [1486], 'coimmunocipitated': [1487], 'Myc-β-TrCP1': [1489], 'antiserum': [1490], '1B,': [1492, 1523], 'lane': [1493, 2053], '4)': [1494], 'when': [1495, 2159], 'overexpressed.': [1499], 'Potential': [1500, 1981], 'interactions': [1501], 'Smad3': [1507, 1521, 1962, 2419, 2422, 2450, 2776], 'examined.': [1510], 'nondetectable': [1513], 'lanes': [1524, 1680, 1743, 1790, 1958, 2014], '2': [1525, 1791, 2015], '3).': [1527, 1793, 2017], 'further': [1529, 1698, 2045, 2157, 2237, 3016], 'investigate': [1530, 1821], 'via': [1536, 1586], 'immunoprecipitation': [1538], 'assays': [1539, 2462], 'silenced': [1546], 'RNA': [1551], '(siRNA)': [1552], 'from': [1553, 2287], 'DNA': [1554], 'templates': [1555], 'vivo.': [1557], 'cotransfection': [1564], 'higher': [1566], 'dose': [1567], 'BS/U6/Smad4': [1569], 'plasmid': [1570, 2251], 'irrelevant': [1575, 1774], 'plasmid,': [1577, 3033, 3040, 3050, 3057], 'BS/U6/GFP': [1578, 1731], '1C),': [1580], 'coimmunoprecipited': [1592], 'β-TrCP1,': [1595, 2371], 'treatment': [1600], 'significantly': [1601, 1691], 'enhances': [1602, 2073, 2791], '1D).': [1606], 'Taken': [1607], 'together,': [1608], 'β-TrCP1.': [1619, 2832, 2935], 'steady-state': [1633], 'level': [1634, 1760, 1782], 'FLAG-tagged': [1637, 1829, 2010], 'co-transfected': [1640, 1827], 'into': [1641, 1832, 2735], 'single': [1647], '(Roc1,': [1653, 1929], 'Cul1,': [1654, 1930, 1999], 'β-TrCP1)': [1656, 1932, 2000], 'combinations': [1660], 'components.': [1663], 'While': [1664, 2954], 'individual': [1665], 'did': [1671, 1777, 2451], 'affect': [1673], '2A,': [1679], '2–4),': [1681], 'Cul1': [1682, 3068], 'Roc1': [1684, 2409, 3055], '(lanes': [1687], '6': [1688], '8)': [1690], 'reduces': [1692], 'expression.': [1694, 1724], 'decreased': [1699, 1860, 1961], 'presence': [1702, 1802, 1854, 1911, 1948, 1977, 2780], 'all': [1704, 2083], 'three': [1705], 'components': [1706, 1825, 1997, 2136, 2256], '(lane': [1707, 1964, 1973], '9).': [1708], 'attempted': [1710], 'siRNA': [1720, 1775, 2250], 'would': [1721, 2099, 2523], 'increase': [1722, 2223], 'transfected': [1728], 'BS/U6/β-TrCP1': [1733, 1735], 'plasmid.': [1734, 3070], 'dose-dependently': [1736], '2B,': [1742, 1789], '4': [1744], '5': [1746], 'panel).': [1750, 1771, 1869, 1891], 'contrast': [1752], 'effect': [1755, 1969, 2019, 2174, 2242, 2958], 'siRNA-transfected': [1768], '(second': [1770], 'BS/U6/GFP,': [1772], 'control,': [1776], 'change': [1779], 'observed': [1795], 'low': [1796], 'levels': [1799], 'suggested': [1806], 'might': [1811, 2925], 'led': [1813], 'turnover': [1817], 'possibility,': [1823], 'plasmids': [1831, 2118, 2139], 'pulse-chase': [1846], 'assays.': [1847, 1993], '35S-labeled': [1849, 1872], 'rapidly': [1859], 'disappeared': [1862], '16': [1864, 1888], '2C,': [1867], 'lower': [1868], 'contrast,': [1871], 'overexpression': [1878], 'considerably': [1880], 'more': [1881], 'stable': [1882], 'still': [1885], 'present': [1886], '(upper': [1890], 'half-life': [1893, 1906], 'absence': [1898], '∼16': [1902], 'h,': [1903], 'whereas': [1904], '∼7': [1915], '2D).': [1918], 'degraded': [1924], 'R-Smads.': [1925], 'cotransfected': [1934, 2133, 2253], 'version': [1937], 'Smad2,': [1939], 'Smad3,': [1940], 'TGF-β.': [1950], 'down-regulated': [1953], '2E,': [1957], '6),': [1959], 'modestly': [1960], '4),': [1965], '2)': [1974], 'stimulation.': [1980], 'Overexpression': [1994], '(Roc-1,': [1998], 'promotes': [2004], 'ectopically': [2008], 'expressed': [2009], '2F,': [2013], 'SCFβ-TrCP1-induced': [2023], 'adding': [2029], 'culture': [2033], '24': [2035], 'transfection.': [2039], 'enhanced': [2046], '2G,': [2052], '4).': [2054], 'Collectively,': [2055], 'data': [2057, 2769, 2937], 'strongly': [2058], 'specific': [2064], 'only': [2079], 'partner': [2081], 'known': [2089], 'activator': [2092], 'R-Smad-induced': [2094], 'transcription.': [2096], 'Conventional': [2097], 'views': [2098], 'predict': [2100], 'that,': [2101], 'because': [2102], 'targets': [2104], 'destruction,': [2107], 'should': [2109], 'down-regulate': [2110], 'Smad4-mediated': [2111], 'activity.': [2113, 2153], 'Different': [2114], 'response': [2116, 2130], 'reporter': [2117], '(SBE),': [2119], '4-luc': [2120], '(four': [2121], 'repeats': [2122], 'element),': [2126], 'ARE-luc': [2128], '(Activin': [2129], 'element)': [2131], 'and/or': [2137], 'Mv1Lu': [2141], 'TGF-β-induced': [2151, 2944], 'Transcriptional': [2154], '3,': [2167], 'B).': [2170], 'clarify': [2172], 'biological': [2179, 2945], 'activity,': [2181], 'distribution': [2183], 'population': [2187], 'G1': [2190, 2228], 'phases': [2193], 'analyzed': [2199], 'flow': [2201, 2263], 'cytometry': [2202, 2264], 'following': [2203], 'As': [2207], 'shown': [2208], 'Fig.': [2210, 2267], '3C,': [2211], 'arrested': [2215], 'TGF-β1': [2217], 'treatment,': [2218], 'judged': [2220], 'phase.': [2229], 'TGF-β1-induced': [2232], 'arrest.': [2235, 2284, 2953], 'inhibitory': [2241, 2276], 'performed.': [2266], '3D': [2268], 'shows': [2269], 'silencing': [2273], 'effects': [2277, 2973], 'TGF-β-mediated': [2281], 'conclude': [2286], 'experiments': [2289], 'Oncogene': [2301], 'Ras': [2302], '(15Saha': [2303], 'Datta': [2305], 'P.K.': [2306], 'Beauchamp': [2307], 'R.D.': [2308], '276:': [2313], '29531-29537Abstract': [2314], '(104)': [2322], 'been': [2354], 'mediate': [2357, 2524], 'mechanisms': [2363], 'clear.': [2366], 'Here': [2367], 'show': [2369], 'This': [2390], 'defines': [2391], 'new': [2393], 'SCFβ-TrCP1-mediated': [2398], 'pathway.': [2407], 'Previously,': [2408], '(16Fukuchi': [2425], 'Imamura': [2427], 'Chiba': [2429], 'Ebisawa': [2431], 'Kawabata': [2433], 'Tanaka': [2435], 'Miyazono': [2437], '12:': [2443], '1431-1443Crossref': [2444], '(177)': [2447], 'our': [2459], 'Smad4-dependent.': [2484, 2977], 'subunit,': [2495], 'between': [2499, 2816, 2850], 'subunit': [2501], 'essential': [2507], 'most': [2515], 'likely': [2516], 'β-TrCP1/Smad,': [2518], 'Roc-1/Smad,': [2521], 'DSGφXS': [2531], 'motif': [2532, 2673, 2683], '7Winston': [2562], '8Lang': [2584], '9Lassot': [2615], '10Busino': [2642], 'There': [2669], 'similar': [2672], '"DLSGLTLQS."': [2677], 'phosphorylation': [2687], 'site': [2688], 'recognition.': [2691], 'stimulation': [2693, 2712], 'One': [2703], 'possibility': [2704], 'upon': [2710], 'may': [2713, 2750, 3010], 'play': [2714], 'some': [2715, 3012], 'event.': [2719], 'Upon': [2720], 'signaling,': [2725], 'R-Smad': [2726], 'forms': [2727], 'heteromeric': [2729, 2747], 'translocates': [2734], 'where': [2738], 'R-Smad/Smad4': [2746], 'formation': [2749, 2802], 'enhance': [2752], 'trigger': [2760], 'complex.': [2767], 'Our': [2768, 2936], 'induced': [2774], 'modest': [2775], 'providing': [2783], 'evidence': [2785], 'hypothesis.': [2788], 'association.': [2794], 'serving': [2811], 'adaptor': [2814], 'phosphorylating': [2822], 'promoting': [2825], 'CSN': [2842, 2861], 'whose': [2844], 'interface': [2849], 'signal': [2851], 'transduction': [2852], 'ubiquitin-dependent': [2854], 'proteolysis.': [2855], 'Recent': [2856], 'work': [2857], 'indicates': [2858], 'copurifies': [2862], 'kinases': [2864], 'possesses': [2866], 'kinase': [2867], '17Uhle': [2893], 'Medalia': [2895], 'O.': [2896], 'Waldron': [2897], 'Dumdey': [2899], 'Bech-Otschir': [2903], 'Berse': [2907], 'Sperling': [2909], 'Schade': [2911], '22:': [2916], '1302-1312Crossref': [2917], '(173)': [2920], 'Jab1/CSN': [2924], 'promote': [2929], 'efficiently': [2942], 'impairs': [2943], 'events': [2946], 'little': [2957], 'progression': [2963], 'those': [2965], 'Loss': [2978], 'pivotal': [2983], 'resistance': [2987], 'tumorigenesis': [2989], 'associated': [2992], 'poor': [2994], 'prognosis': [2995], 'cancer.': [2997], 'mechanism': [3000], 'underlying': [3001], 'study': [3009], 'provide': [3011], 'useful': [3013], 'information': [3014], 'understanding': [3017], 'pathogenesis': [3019], 'cancer': [3021], 'disease.': [3022], 'grateful': [3025], 'Dr.': [3027, 3034, 3041, 3051], 'Mario': [3028], 'Ascoli': [3029], 'HA-Jab1': [3031], 'Yang': [3035], 'BS/U6': [3039], 'Wade': [3043], 'Myc-tagged': [3047, 3067], 'Kazuhiro': [3052], 'Iwai': [3053], 'Drs.': [3059], 'Kei-ichi': [3060], 'Nakayama': [3061], 'Shigetsugu': [3063], 'Hatakeyama': [3064]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2072688355', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 3}, {'year': 2021, 'cited_by_count': 5}, {'year': 2020, 'cited_by_count': 6}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 8}, {'year': 2014, 'cited_by_count': 6}, {'year': 2013, 'cited_by_count': 6}, {'year': 2012, 'cited_by_count': 5}], 'updated_date': '2024-12-16T11:31:09.709854', 'created_date': '2016-06-24'}
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