Title: The influence of the condition of cells and medium on production of polyhedra of Autographa californica nuclear polyhedrosis virus in vitro
Abstract: A culture of an established cell line of Trichoplusia ni (TN-368) was infected with the nuclear polyhedrosis virus of Autographa californica (ACNPV) at different stages of growth. After 50 hr of growth (early log phase), production of polyhedral inclusion bodies (PIBs) declined rapidly as cell number increased. Medium taken from cultures in the later stages of growth suppressed PIB production in early log-phase cells. Cultures infected with ACNPV and inoculated into fresh medium at high cell concentrations produced relatively fewer PIBs than those inoculated at lower concentrations. The depressive effect of utilized medium is suggested to be the result of exhaustion of a precursor rather than accumulation of an inhibitor.
Publication Year: 1977
Publication Date: 1977-11-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 41
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