Title: THE DETECTION OF CUCUMBER MOSAIC VIRUS IN SINGLE APHIDS
Abstract: Abstract Cucumber mosaic virus (CMV) was detected in single aphids Myzus persicae and Aphis gossy pii by dot immunobinding assay (DIBA). The DIB A procedure with the lowest detectable concentration endpoint of 0.16ng/ml of purified CMV, or 0. 32pg per sample dot of 2μI antigens, satisfied the sensitivity required for the detection of CMV in single virus‐carrying aphids. The aphid extracting method of dipping the stylets dissected from aphid heads in the droplets of small volume (2–5p1) of suitable buffer not only ensured the highly concentrated Firuses fully released from the aphid stylets, but decreased the interference of non‐virus materials from aphids to minimum as well, and thus overcame the two major difficulties in the detection of plant viruses in vectors. The virus‐carrying rates tested by DIBA had good coincidence with the transmission rates by bioassay. Undoubtedly, such breakthroughs in the technique of detecting nonpersistent viruses in aphid vectors are of great epidemiological importance.
Publication Year: 1994
Publication Date: 1994-06-01
Language: en
Type: article
Indexed In: ['crossref']
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