Publication Information

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3987, 4012, 4025, 4028, 4035, 4050, 4053, 4056, 4064, 4081, 4088, 4092, 4098, 4103, 4106, 4114, 4141, 4173, 4178, 4194, 4198, 4207, 4210, 4218, 4224, 4240, 4247, 4252, 4255, 4263, 4268, 4278, 4285, 4371, 4377, 4415, 4439, 4443, 4454, 4457, 4463, 4468, 4475], 'biosynthesis': [12, 264, 530], 'of': [13, 17, 29, 37, 52, 56, 82, 85, 97, 101, 111, 114, 122, 153, 160, 170, 189, 193, 205, 211, 230, 238, 242, 247, 265, 269, 281, 289, 304, 308, 334, 337, 349, 353, 363, 366, 374, 405, 412, 422, 441, 445, 457, 463, 482, 490, 494, 499, 507, 513, 531, 585, 667, 703, 716, 804, 808, 848, 862, 942, 956, 960, 1012, 1021, 1028, 1038, 1093, 1156, 1169, 1176, 1188, 1196, 1206, 1237, 1276, 1324, 1425, 1661, 1845, 1849, 1855, 1891, 1900, 1916, 1975, 1978, 1989, 1997, 2001, 2004, 2008, 2042, 2073, 2088, 2091, 2108, 2148, 2154, 2213, 2225, 2298, 2546, 2560, 2585, 2591, 2597, 2606, 2608, 2638, 2685, 2687, 2758, 2805, 2968, 2978, 3000, 3021, 3110, 3124, 3131, 3135, 3153, 3189, 3227, 3235, 3329, 3416, 3439, 3466, 3469, 3471, 3520, 3528, 3546, 3623, 3640, 3655, 3728, 3736, 3747, 3756, 3780, 3811, 3827, 3838, 3841, 3855, 3858, 3876, 3893, 3897, 3929, 3954, 3970, 3993, 4034, 4052, 4058, 4061, 4094, 4102, 4105, 4121, 4190, 4197, 4209, 4221, 4223, 4239, 4251, 4254, 4267, 4270, 4370, 4376, 4411, 4414, 4419, 4422, 4435, 4442, 4448, 4467], 'peptidoglycan': [15, 267, 577], 'layer': [16, 268], 'bacterial': [19, 271, 533], 'cell.': [20, 272, 2235], 'It': [21, 273, 1788], 'provides': [22, 274], 'attractive': [24, 276], 'template': [25, 277, 2220], 'for': [26, 79, 182, 278, 331, 434, 582, 1067, 1100, 1597, 1828, 1911, 2057, 2140, 2221, 2653, 2895, 2949, 3216, 3223, 3232, 3463, 3524, 3764, 3773, 3816, 3960, 3978, 4008, 4019, 4024, 4291], 'design': [28, 280, 584], 'novel': [30, 282], 'antibiotic': [31, 41, 283, 293, 587, 673], 'drugs': [32, 284, 588], 'and': [33, 127, 201, 244, 285, 379, 453, 496, 505, 719, 723, 925, 958, 1036, 1044, 1073, 1097, 1115, 1182, 1282, 1301, 1427, 1432, 1570, 1587, 1740, 1749, 1800, 1857, 1927, 2013, 2080, 2094, 2114, 2144, 2162, 2201, 2302, 2351, 2353, 2423, 2514, 2522, 2538, 2657, 2711, 2723, 2740, 2779, 2781, 2808, 2882, 2886, 2936, 2940, 3003, 3038, 3150, 3163, 3175, 3211, 3229, 3242, 3249, 3253, 3261, 3323, 3335, 3504, 3744, 3873, 3899, 3903, 3943, 3974, 3985, 4003, 4063, 4124, 4134, 4139, 4156, 4164, 4214, 4246, 4421, 4450, 4462], 'target': [36, 288, 666, 1153], 'naturally': [39, 291, 669], 'occurring': [40, 292, 670], 'fosfomycin,': [42, 294, 3743], 'which': [43, 105, 233, 295, 357, 485, 522, 858, 962, 1064, 1894, 2069, 2900, 2954, 3508, 3628, 4137, 4191, 4276], 'covalently': [44, 296, 697], 'attaches': [45, 297, 698], 'to': [46, 58, 72, 148, 226, 236, 298, 310, 324, 400, 478, 488, 699, 844, 911, 923, 935, 1026, 1051, 1111, 1144, 1184, 1295, 1430, 1441, 1594, 1664, 2048, 2054, 2099, 2188, 2267, 2529, 2621, 2628, 2665, 2677, 2997, 3016, 3398, 3449, 3506, 3512, 3544, 3558, 3589, 3662, 3665, 3777, 3784, 3901, 3908, 3918, 3976, 4049, 4154, 4184, 4202], 'Cys115': [47, 57, 161, 299, 309, 413, 1108, 1581, 1590, 1793, 1829, 2009, 2021, 2034, 2149], 'active': [50, 133, 302, 385, 714, 906, 1444, 1568, 2016, 2119, 2655, 2984, 3133, 4219], 'site': [51, 303, 715, 907, 1445, 1569, 2017, 2656, 3134, 4220], 'enzyme.': [54, 306], 'Mutations': [55, 307], 'Asp': [59, 311], 'exist': [60, 312], 'pathogens': [62, 314], 'such': [63, 315, 1241, 2019], 'as': [64, 162, 316, 414, 874, 931, 1113, 1242, 1830, 2020, 2181, 2219, 2241, 2509, 2693, 3182, 3263, 3269, 3402, 3445, 4165, 4167, 4282], 'Mycobacteria': [65, 317], 'or': [66, 318, 1233, 1666, 3062, 3086, 3997], 'Chlamydia': [67, 319], 'rendering': [68, 320], 'these': [69, 321], 'organisms': [70, 322], 'resistant': [71, 323], 'fosfomycin.': [73, 325, 3653], 'Thus,': [74, 326], 'there': [75, 327], 'a': [77, 150, 163, 190, 228, 329, 402, 415, 442, 480, 704, 919, 932, 949, 1015, 1053, 1147, 1185, 1308, 1557, 1576, 1742, 1825, 1995, 2062, 2129, 2194, 2231, 2311, 2317, 2963, 2991, 2998, 3070, 3252, 3325, 3427, 3446, 3464, 3530, 3539, 3708, 3753, 3938, 3951, 3990, 4432], 'need': [78, 330], 'elucidation': [81, 333], 'role': [84, 336, 1827, 2007, 2147], 'this': [86, 338, 566, 2028, 2125, 2138], 'cysteine': [87, 179, 339, 431, 705, 1663, 2076, 3327], 'reaction.': [91, 155, 343, 407, 1107, 3549, 3787], 'We': [92, 344, 2060, 2133, 3455, 3582], 'determined': [93, 345, 2171, 3879], 'x-ray': [95, 347, 2097], 'structure': [96, 117, 241, 348, 369, 493, 1884, 2139, 2451, 2809, 2888, 2942, 3011, 3146], 'C115S': [99, 351, 2185, 3419, 3433, 3617, 3669, 3700, 3748, 3889, 3946, 4077], 'mutant': [100, 352, 1848, 1923, 2064, 2083, 2126, 2263, 3618, 3641, 3670, 3988, 4115, 4142], 'Enterobacter': [102, 354, 720], 'cloacae': [103, 355, 721, 1586, 2067, 2191, 3418, 3475], 'MurA,': [104, 206, 356, 458, 1556, 2068, 3627], 'was': [106, 358, 1109, 1908, 2170, 2186, 2217, 2228, 2239, 2265, 2279, 2452, 2624, 2691, 2761, 2785, 3421, 3534, 3556, 3759, 3771, 3793, 3806, 3822, 3833, 3847, 3868, 3878, 3934, 3965, 4001, 4014, 4041, 4046, 4069], 'crystallized': [107, 359, 1851, 2081, 2280], 'presence': [110, 362, 1854, 2087, 2297], 'substrates': [113, 365, 901, 2090], 'MurA.': [115, 367, 1094, 2226, 3114, 3136], 'The': [116, 368, 1046, 1204, 1905, 2183, 2204, 2236, 2326, 2450, 2672, 2708, 2986, 3009, 3089, 3115, 3137, 3185, 3239, 3496, 3550, 3615, 3788, 3831, 3863, 3963, 4232, 4409], 'depicts': [118, 370], 'product': [120, 183, 195, 250, 372, 435, 447, 502, 2058, 2106, 3111, 3178, 3255, 3452, 4026], 'state': [121, 196, 200, 204, 373, 448, 452, 456, 2107, 3256, 4375], 'with': [125, 197, 218, 377, 449, 470, 948, 1551, 2111, 2157, 2177, 2358, 2601, 2632, 2728, 2763, 2787, 2970, 3195, 3266, 3324, 3426, 3500, 3631, 3882, 4031, 4076, 4109, 4172, 4258, 4368, 4431], 'enolpyruvyl-UDP-N-acetylglucosamine': [126, 378, 2112], 'inorganic': [128, 219, 380, 471, 1029], 'phosphate': [129, 220, 381, 472, 1030, 1207, 3126, 3165, 3173, 3240, 3883], 'trapped': [130, 382, 2116, 2980, 3048, 3922, 4216], 'site.': [134, 386, 2120, 2985], 'Kinetic': [135, 387, 2121, 3412], 'analysis': [136, 388, 1844, 2098, 2122, 4201], 'revealed': [137, 389, 1885, 2104, 2580, 4206], 'that': [138, 146, 176, 209, 390, 398, 428, 461, 1792, 2033, 2124, 2581, 4369, 4453], 'Cys-to-Ser': [140, 392], 'mutation': [141, 393, 1660, 1744, 2184, 2224], 'results': [142, 394], 'appears': [147, 225, 399, 477, 2053], 'perform': [149, 401], 'single': [151, 403, 1309, 2130, 2312], 'turnover': [152, 404, 2131], 'Opposing': [156, 408], 'common': [158, 410], 'view': [159, 411, 3090, 3116], 'key': [164, 416], 'residue': [165, 417, 1240, 2018], 'chemical': [168, 420, 1983], 'reaction': [169, 421, 944, 1014, 1055, 1837, 1889, 1919, 1981, 3498, 3709, 3821, 3832, 3941, 3964, 3984, 4013, 4054, 4089, 4099, 4373], 'transfer,': [172, 424], 'we': [173, 207, 425, 459, 2030, 3436, 3646], 'now': [174, 426], 'conclude': [175, 427], 'wild-type': [178, 430, 2075, 2189, 2215, 3650], 'release': [184, 246, 436, 498, 3112, 3179, 3592, 3909], 'only.': [185, 437, 2132], 'On': [186, 438], 'basis': [188, 440], 'detailed': [191, 443], 'comparison': [192, 444, 3881, 4447], 'intermediate': [199, 451, 947, 1890, 1915, 1977, 4374], 'unliganded': [203, 455, 2458], 'propose': [208, 460], 'dissociation': [210, 462], 'products': [213, 465, 2981, 3023, 3049, 3911, 3923, 4208, 4233, 4256], 'ordered': [216, 468], 'event': [217, 469], 'leaving': [221, 473], 'first.': [222, 474], 'Phosphate': [223, 475], 'departure': [224, 476], 'trigger': [227, 479], 'suite': [229, 481, 2363], 'conformational': [231, 483], 'changes,': [232, 484], 'finally': [234, 486], 'leads': [235, 487, 1183], 'opening': [237, 489], 'two-domain': [240, 492], 'second': [249, 501, 3551, 3561], 'enolpyruvyl-UDP-N-acetylglucosamine.': [251, 503], 'Growth': [504], 'survival': [506], 'bacteria': [508], 'relies': [509], 'on': [510, 1172, 2316, 3473], 'functionality': [512], '2.5.1.7),': [521], 'catalyzes': [523, 801, 2128], 'first': [525, 1158], 'committed': [526], 'step': [527, 941, 1011], 'cell': [534, 2812], 'wall': [535], '(1van': [536], 'Heijenoort': [537, 552], 'J.': [538, 553, 611, 992, 1221, 1253, 1514, 1628, 1778, 1867, 1939, 1958, 2394, 2480, 2732, 2767, 3294, 3359, 3486, 4327, 4393], 'Ghuysen': [539], 'J.M.': [540], 'Hacken-beck': [541], 'R.': [542, 1077, 1934], 'Bacterial': [543], 'Cell': [544], 'Wall.': [545], '27.': [546], 'Elsevier,': [547], 'Amsterdam1994:': [548], '39-54Google': [549], 'Scholar,': [550, 602, 619, 636, 750, 773, 983, 1365, 1394, 1475, 1504, 1523, 1622, 1637, 1692, 1771, 1949, 4318, 4342], '2van': [551], 'Nat.': [554], 'Prod.': [555], 'Rep.': [556], '2001;': [557, 1387, 1497], '18:': [558], '503-519Crossref': [559], 'PubMed': [560, 598, 617, 632, 647, 691, 746, 769, 795, 894, 979, 1003, 1086, 1135, 1228, 1264, 1361, 1390, 1418, 1471, 1500, 1519, 1544, 1618, 1633, 1654, 1688, 1707, 1735, 1767, 1783, 1819, 1878, 1965, 2257, 2373, 2418, 2444, 2504, 2703, 2751, 2774, 2799, 3206, 3318, 3383, 3491, 3577, 3610, 3693, 4314, 4338, 4357, 4404], 'Scopus': [561, 599, 633, 648, 692, 747, 770, 796, 895, 980, 1004, 1087, 1136, 1229, 1265, 1362, 1391, 1419, 1472, 1501, 1520, 1545, 1619, 1634, 1655, 1689, 1708, 1736, 1768, 1784, 1820, 1879, 1946, 1966, 2258, 2374, 2419, 2445, 2505, 2704, 2752, 2775, 2800, 3207, 3319, 3384, 3492, 3578, 3611, 3694, 4315, 4339, 4358, 4405], '(355)': [562], 'Google': [563, 601, 618, 635, 650, 694, 749, 772, 798, 897, 982, 1006, 1089, 1138, 1231, 1267, 1364, 1393, 1421, 1474, 1503, 1522, 1547, 1621, 1636, 1657, 1691, 1710, 1738, 1770, 1786, 1822, 1881, 1948, 1968, 2260, 2376, 2421, 2447, 2507, 2706, 2738, 2754, 2777, 2802, 3209, 3321, 3386, 3494, 3580, 3613, 3696, 4317, 4341, 4360, 4407], 'Scholar).': [564, 651, 695, 799, 898, 1007, 1139, 1268, 1422, 1548, 1711, 1787, 1823, 1882, 2261, 2707, 3387, 3495, 3581, 3614, 3697, 4361, 4408], 'Because': [565], 'pathway': [567, 1070], 'does': [568, 4117], 'not': [569, 2023, 2036, 3673, 4118, 4145], 'occur': [570], 'mammalian': [572], 'organisms,': [573], 'enzymes': [574], 'involved': [575, 1103, 2038, 3171], 'synthesis': [578, 2996], 'are': [579, 2714, 2884, 2938, 3024, 3030, 3035, 3041, 3050, 3058, 3156, 3180, 3244], 'ideal': [580, 2846, 2912], 'targets': [581], 'new': [586, 2964], '(3Green': [589], 'D.W.': [590], 'Expert': [591], 'Opin.': [592], 'Ther.': [593], 'Targets.': [594], '2002;': [595, 614, 3607], '6:': [596], '1-19Crossref': [597], '(117)': [600], '4McDevitt': [603], 'D.': [604], 'Payne': [605], 'D.J.': [606, 608, 2745, 3200], 'Holmes': [607], 'Rosenberg': [609], 'M.': [610, 1214, 1951, 2396, 2432, 2482, 3296, 3361], 'Appl.': [612, 2733], 'Microbiol.': [613, 628], '92:': [615], '28S-34SCrossref': [616], '5El': [620], 'Zoeiby': [621], 'A.': [622, 777, 779, 1357, 1386, 1402, 1453, 1455, 1496, 1540, 1758, 1936, 2790, 4296, 4298], 'Sanschagrin': [623], 'F.': [624, 1404, 1698, 2248], 'Levesque': [625], 'R.C.': [626, 1775], 'Mol.': [627, 1081, 2768], '2003;': [629, 644, 995, 1225, 1256, 1780, 1870, 1962, 4330, 4396], '47:': [630, 2442], '1-12Crossref': [631], '(274)': [634], '6Katz': [637], 'A.H.': [638], 'Caufield': [639], 'C.E.': [640], 'Curr.': [641], 'Pharm.': [642], 'Des.': [643], '9:': [645], '857-866Crossref': [646], '(67)': [649], 'particular': [654], 'has': [655, 1591, 1789, 1838], 'received': [656], 'attention': [657], 'past': [660], 'because': [661, 1659, 2000, 2014, 4101], 'broad': [671], 'spectrum': [672], 'fosfomycin': [674, 3472, 3658], '(7Kahan': [675, 730], 'F.M.': [676, 731], 'Kahan': [677, 732], 'J.S.': [678, 733, 2392, 2478, 3292, 3357], 'Cassidy': [679, 734], 'P.J.': [680, 735, 2731], 'Kropp': [681, 736], 'H.': [682, 737], 'Ann.': [683, 738], 'N.': [684, 739, 1375, 1406, 1485, 1510, 1533, 1626, 1702, 2252, 3482], 'Y.': [685, 740, 760, 1609, 3684], 'Acad.': [686, 741, 1353, 1382, 1492, 1536], 'Sci.': [687, 742, 1354, 1383, 1493, 1537], '1974;': [688, 743], '235:': [689, 744], '364-386Crossref': [690, 745], '(616)': [693, 748], 'Fosfomycin': [696], 'thiol': [701], 'group': [702, 847, 955, 1155, 2566], '(position': [706], '115': [707, 1718], 'Escherichia': [709], 'coli': [710, 718, 1119, 2234, 3626], 'numbering)': [711], 'E.': [717, 991, 1118, 1252, 1367, 1396, 1408, 1477, 1506, 1525, 1585, 1588, 1694, 1866, 2066, 2190, 2233, 2244, 2698, 3417, 3474, 3478, 3605, 3625, 4326, 4392], 'thus': [724, 4125], 'irreversibly': [725], 'inhibits': [726], 'enzymatic': [728, 4211], 'function': [729, 1112], '8Marquardt': [751], 'J.L.': [752, 1601, 3676], 'Brown': [753, 1602, 3677], 'E.D.': [754, 1603, 3678], 'Lane': [755, 1604, 1644, 1678, 1725, 3679], 'W.S.': [756, 1605, 1645, 1679, 1726, 3680], 'Haley': [757, 1606, 3681], 'T.M.': [758, 1607, 3682], 'Ichikawa': [759, 1608, 3683], 'Wong': [761, 1610, 3685], 'C.H.': [762, 1611, 3686], 'Walsh': [763, 973, 1127, 1612, 1648, 1682, 1729, 1811, 3687, 4349], 'C.T.': [764, 879, 974, 1128, 1613, 1649, 1683, 1730, 1812, 1932, 3688, 4350], 'Biochemistry.': [765, 975, 1131, 1614, 1650, 1684, 1703, 1731, 1815, 2253, 3689, 4353], '1994;': [766, 1615, 3690], '33:': [767, 1616, 3691], '10646-10651Crossref': [768, 1617, 3692], '(134)': [771, 1620, 3695], '9Skarzynski': [774], 'T.': [775, 1122, 1451, 1806, 2404, 2490, 3304, 3369, 4294, 4344], 'Mistry': [776, 1452, 4295], 'Wonacott': [778, 1454, 4297], 'Hutchinson': [780, 1456, 4299], 'S.E.': [781, 1457, 4300], 'Kelly': [782, 1458, 4301], 'V.A.': [783, 1459, 4302], 'Duncan': [784, 1129, 1460, 1646, 1680, 1727, 1759, 1813, 4303, 4351], 'K.': [785, 1130, 1461, 1529, 1647, 1681, 1700, 1728, 1760, 1814, 2250, 4304, 4352], 'Structure.': [786, 1409, 1462, 4305], '1996;': [787, 888, 976, 1410, 1463, 1651, 1685, 1732, 4306], '4:': [788, 1411, 1464, 4307], '1465-1474Abstract': [789, 1465, 4308], 'Full': [790, 792, 891, 998, 1000, 1259, 1261, 1413, 1415, 1466, 1468, 1873, 1875, 4309, 4311, 4333, 4335, 4399, 4401], 'Text': [791, 793, 892, 999, 1001, 1260, 1262, 1414, 1416, 1467, 1469, 1874, 1876, 4310, 4312, 4334, 4336, 4400, 4402], 'PDF': [794, 893, 1002, 1263, 1417, 1470, 1877, 4313, 4337, 4403], '(202)': [797, 1473, 4316], 'transfer': [803, 1106, 1798, 1992, 2041], 'moiety': [807, 1199, 2045, 4470], 'phosphoenol': [809], 'pyruvate': [810, 4155], '(PEP)': [811], '1The': [812, 828], 'abbreviations': [813, 829], 'used': [814, 830, 2218, 3458, 3557, 3643, 3917], 'are:': [815, 831], 'PEP,': [816, 832, 918, 924, 961, 1145], 'phosphoenolpyruvate;': [817, 833], 'THI,': [818, 834], 'tetrahedral': [819, 835, 965, 1034, 1202, 1888, 1914, 4372], 'intermediate;': [820, 836], 'UNAG,': [821, 837, 3740], 'UDP-N-acetylglucosamine;': [822, 838], 'EP-UNAG,': [823, 839, 1281], 'enolpyruvyl-UDP-N-acetylglucosamine;': [824, 840], 'MES,': [825, 841, 2287], '4-morpholineethanesulfonic': [826, 842], 'acid.': [827, 843], '3′-hydroxyl': [846, 954], 'UDP-N-acetylglucosamine': [849], '(UNAG)': [850], '(Fig.': [851, 908, 4188, 4230], '1).': [852], 'This': [853, 939, 1578, 1883, 2578, 3536, 3933, 4273], 'chemically': [854, 2669], 'unusual': [855, 1305], 'reaction,': [856, 4212], 'C-O': [860], 'bond': [861, 1041, 4459], 'PEP': [863, 1023, 1148, 1178, 1198, 1802, 1858, 1903, 2047, 2159, 3505, 3783, 3900, 3973, 4086, 4148, 4469], 'cleaved': [865], 'rather': [866], 'than': [867], 'high': [869, 3638, 4110, 4161], 'energy': [870], 'P-O': [871], 'bond,': [872], 'runs': [873], 'addition-elimination': [876, 1836], 'process': [877], '(10Walsh': [878], 'Benson': [880], 'T.E.': [881], 'Kim': [882, 1123, 1807, 4345], 'D.H.': [883, 968, 1124, 1639, 1673, 1720, 1808, 4346], 'Lees': [884, 971, 1125, 1640, 1674, 1721, 1809, 4347], 'W.J.': [885, 972, 1126, 1641, 1675, 1722, 1810, 4348], 'Chem.': [886, 994, 1223, 1255, 1869, 1941, 1960, 2414, 2440, 2500, 3314, 3379, 4329, 4395], 'Biol.': [887, 993, 1082, 1254, 1868, 4328, 4394], '3:': [889], '83-91Abstract': [890], '(48)': [896], 'After': [899], 'have': [902, 3456, 3583], 'docked': [903], 'into': [904, 1313, 2556], '2),': [909], 'adhering': [910], 'strict': [913], 'binding': [914, 1424, 3174], 'order': [915], 'UNAG': [916, 957, 1161, 1426, 1856, 2049, 2093, 2163, 2301, 3503, 3898], 'before': [917, 3819], 'proton': [920, 1016, 1141, 1797], 'added': [922, 934, 1143, 2664, 4002], 'resulting': [927], 'oxocarbenium': [928, 1149, 1179], 'ion': [929, 1180, 3166, 3241], 'is,': [930], 'whole,': [933], 'sugar': [937], 'nucleotide.': [938], 'addition': [940, 1294, 3779, 3826, 3837, 3854, 3969, 4057], 'yields': [945], 'covalent': [950, 1186, 3663, 4458], 'link': [951], 'between': [952, 1042, 2648, 4460], 'C-2': [959, 1043, 1174, 1194, 4461], 'then': [963, 3510], 'adopts': [964], 'configuration': [966, 1907, 1974], '(11Kim': [967], 'Tucker-Kellogg': [969], 'G.W.': [970], '35:': [977, 1652, 1686, 1733], '5435-5440Crossref': [978], '(25)': [981], '12Eschenburg': [984, 4319], 'S.': [985, 1246, 1356, 1369, 1385, 1398, 1400, 1479, 1495, 1527, 1539, 1696, 1860, 2246, 2696, 3597, 4320, 4386], 'Kabsch': [986, 1247, 1378, 1488, 1530, 1861, 4321, 4387], 'W.': [987, 1248, 1379, 1489, 1531, 1862, 2367, 4322, 4388], 'Healy': [988, 1249, 1863, 3598, 4323, 4389], 'M.L.': [989, 1250, 1864, 3599, 4324, 4390], 'Schonbrunn': [990, 1251, 1865, 2697, 3604, 4325, 4391], '278:': [996, 1257, 1871, 4331, 4397], '49215-49222Abstract': [997, 1258, 1872, 4332, 4398], '(72)': [1005, 1266, 1880, 4340, 4406], 'In': [1008, 1293, 1549, 2027, 3798], 'elimination': [1010, 1205], 'removed': [1018], 'from': [1019, 1032, 1561, 1584, 1747, 1801, 2046, 2065, 2137, 2166, 2199, 2284, 2310, 2619, 2845, 2903, 2911, 2957, 2990, 3073, 3092, 3113, 3593, 4129], 'C-3': [1020], 'moiety,': [1024], 'leading': [1025], 'separation': [1027], '(Pi)': [1031], 'adduct': [1035, 1187], 'reformation': [1037], 'double': [1040], 'C-3.': [1045], 'only': [1047, 4428], 'other': [1048, 1440], 'known': [1050], 'catalyze': [1052], 'similar': [1054, 1315], 'AroA': [1057, 1302, 1918, 1980], '(also': [1058], 'called': [1059], '5-enolpyruvylshikimate-3-phosphate': [1060, 3594], 'synthase,': [1061], '2.5.1.19),': [1063], 'vital': [1066], 'shikimate': [1069, 1428], 'plants': [1072], 'numerous': [1074], 'microorganisms': [1075], '(13Bentley': [1076], 'Crit.': [1078], 'Rev.': [1079], 'Biochem.': [1080, 1515, 1629, 3487, 3573], '1990;': [1083], '25:': [1084], '307-384Crossref': [1085], '(607)': [1088], 'Scholar).Fig.': [1090], '2Addition-elimination': [1091], 'mechanism': [1092, 1270, 2143], 'enzyme-X,': [1095], 'enzyme-Y,': [1096], 'enzyme-Z': [1098, 1116], 'stand': [1099], 'side': [1101, 3225], 'chains': [1102], 'proposed': [1110], 'enzyme-Y': [1114], '(24Skarzynski': [1121, 1805], '1998;': [1132, 1516, 1816, 2415, 2501, 3315, 3380, 3488, 4354], '37:': [1133, 1817, 4355], '2572-2577Crossref': [1134, 1818, 4356], '(63)': [1137, 1821, 4359], 'A': [1140, 3767, 4043], 'yielding': [1146], 'ion,': [1150], 'whereas': [1151, 3667], 'hydroxyl': [1154], 'bound': [1159], 'substrate': [1160], 'gets': [1162], 'deprotonated': [1163], '(A),': [1164], 'followed': [1165], 'by': [1166, 1321, 1842, 1982, 2336, 2454, 3515, 3657, 3824, 3835, 3852, 3880, 3936, 3967, 4055, 4427], 'nucleophilic': [1167], 'attack': [1168], 'oxyanion': [1171], 'atom': [1175, 1195, 2833, 3017, 4466], '(B),': [1181], 'two': [1190, 1314, 1436, 2589, 2603, 3440, 4192], 'substrates,': [1191], 'where': [1192, 2866, 2880, 2921, 2934], 'configuration.': [1203], 'may': [1208, 2035], 'proceed': [1209, 3977], 'through': [1210, 1234], 'self-catalysis': [1211], '(C1)': [1212], '(26An': [1213], 'Maitra': [1215, 1952], 'U.': [1216, 1218, 1355, 1384, 1494, 1538, 1953, 1955], 'Neidlein': [1217, 1954], 'Bartlett': [1219, 1926, 1937, 1956], 'P.A.': [1220, 1938, 1957, 3565], 'Am.': [1222, 1940, 1959], 'Soc.': [1224, 1942, 1961], '125:': [1226, 1963], '12759-12767Crossref': [1227, 1964], '(15)': [1230, 1967], 'Scholar)': [1232, 1739, 1969, 2508, 2739, 2778, 3210, 3322], 'action': [1236, 3470], 'Asp305': [1243], '(C2)': [1244], '(12Eschenburg': [1245, 1859, 4385], 'Either': [1269], 'would': [1271, 3629], 'result': [1272], 'formation': [1275], 'vinyl': [1278], 'ether': [1279], 'product,': [1280, 3562], 'Pi': [1283, 2115, 3563, 3591, 3877, 3904, 4095], '(D).View': [1284], 'Large': [1285, 3099, 3404], 'Image': [1286, 3100, 3405], 'Figure': [1287, 3101, 3406], 'ViewerDownload': [1288, 3102, 3407], 'Hi-res': [1289, 3103, 3408], 'image': [1290, 2320, 3104, 3409], 'Download': [1291, 3105, 3410], '(PPT)': [1292, 3411], 'their': [1296], 'strong': [1297], 'mechanistic': [1298], 'similarity,': [1299], 'share': [1303], 'protein': [1306, 2238, 2640, 4111, 4269], 'architecture:': [1307], 'polypeptide': [1310, 4444], 'chain': [1311, 3226], 'folds': [1312], 'globular': [1316, 2604], 'domains,': [1317], 'each': [1318, 1439, 2607, 2686, 4222], 'built': [1319], 'up': [1320], '3-fold': [1322], 'repetition': [1323], 'same': [1326, 1972, 3395, 3813, 4082], 'βαβαββ': [1327], 'motif': [1328], '(14Stallings': [1329], 'W.C.': [1330], 'Abdel-Meguid': [1331], 'S.S.': [1332], 'Lim': [1333], 'L.W.': [1334], 'Shieh': [1335], 'H.S.': [1336], 'Dayringer': [1337], 'H.E.': [1338], 'Leimgruber': [1339], 'N.K.': [1340], 'Stegeman': [1341], 'R.A.': [1342], 'Anderson': [1343], 'K.S.': [1344], 'Sikorski': [1345], 'J.A.': [1346], 'Padgette': [1347], 'S.R.': [1348], 'Kishore': [1349], 'G.M.': [1350, 2384, 2470, 3284, 3349], 'Proc.': [1351, 1380, 1490, 1534], 'Natl.': [1352, 1381, 1491, 1535], '1991;': [1358, 2441, 2735, 2796], '88:': [1359], '5046-5050Crossref': [1360], '(133)': [1363], '15Schonbrunn': [1366, 1476], 'Eschenburg': [1368, 1399, 1478, 1526, 1695, 2245], 'Shuttleworth': [1370, 1480], 'W.A.': [1371, 1481], 'Schloss': [1372, 1482], 'J.V.': [1373, 1483], 'Amrhein': [1374, 1405, 1484, 1509, 1532, 1625, 1701, 2251, 3481], 'Evans': [1376, 1486], 'J.N.': [1377, 1487], '98:': [1388, 1498], '1376-1380Crossref': [1389, 1499], '(382)': [1392, 1502], '16Schonbrunn': [1395], 'Sack': [1397], 'Perrakis': [1401], 'Krekel': [1403, 1697, 2247], 'Mandelkow': [1407], '1065-1075Abstract': [1412], '(88)': [1420], 'Upon': [1423], '3-phosphate': [1429], 'AroA,': [1433], 'respectively,': [1434], 'domains': [1437, 2605], 'approach': [1438], 'establish': [1442], 'interdomain': [1448, 1573], 'cleft': [1449, 1574], '(9Skarzynski': [1450, 4293], '17Schonbrunn': [1505], 'Svergun': [1507, 3479], 'D.I.': [1508, 3480], 'Koch': [1511, 3483], 'M.H.': [1512, 3484], 'Eur.': [1513, 1627, 3485], '253:': [1517, 3489], '406-412Crossref': [1518, 3490], '(50)': [1521, 3493], '18Schonbrunn': [1524], 'Luger': [1528, 1699, 2249], '2000;': [1541, 1704, 2254, 2700], '97:': [1542], '6345-6349Crossref': [1543], '(144)': [1546], 'concert': [1550], 'domain': [1553, 1564, 2152, 3401], 'movement': [1554, 2153], '10-residue': [1558, 3096], 'loop,': [1559], 'Pro112-Pro121,': [1560], 'top': [1563, 2757], 'moves': [1565], 'toward': [1566, 1799, 3094], 'closes': [1571], 'like': [1575], 'lid.': [1577], 'loop': [1579, 3097, 3138, 3219], 'hosts': [1580], 'coli.': [1589], 'been': [1592, 1790, 1840], 'shown': [1593, 3025, 3051, 3078, 3141, 3181], 'be': [1595, 2037, 2055, 2553, 3925, 4127], 'critical': [1596], 'activity': [1599, 3415, 3527, 3702], '(8Marquardt': [1600, 3675], '19Wanke': [1623], 'C.': [1624], '1993;': [1630], '218:': [1631], '861-870Crossref': [1632], '(65)': [1635], '20Kim': [1638], 'Kempsell': [1642, 1676, 1723, 1755], 'K.E.': [1643, 1677, 1724, 1756], '4923-4928Crossref': [1653, 1687, 1734], '(156)': [1656, 1690, 1737], 'Scholar),': [1658, 2377, 2422, 2448, 2755], 'serine': [1665, 2071, 3330], 'alanine': [1667], 'renders': [1668], 'inactive': [1671], '(20Kim': [1672, 1719], '21Schonbrunn': [1693], '39:': [1705, 2255], '2164-2173Crossref': [1706, 2256], '(40)': [1709, 2259], 'However,': [1712, 1985], 'aspartate': [1713], 'tolerated': [1715], 'position': [1717, 2078, 3332, 3396], 'natural': [1743], 'Mycobacterium': [1748], 'Clamydiae': [1750], 'species': [1751], '(22De': [1752], 'Smet': [1753], 'K.A.': [1754, 2792], 'Gallagher': [1757], 'Young': [1761], 'D.B.': [1762], 'Microbiology.': [1763], '1999;': [1764], '145:': [1765], '3177-3184Crossref': [1766], '(111)': [1769], '23McCoy': [1772], 'A.J.': [1773], 'Sandlin': [1774], 'Maurelli': [1776], 'A.T.': [1777, 2380, 2466, 3280, 3345], 'Bacteriol.': [1779], '185:': [1781], '1218-1228Crossref': [1782], '(91)': [1785], 'suggested': [1791], 'participates': [1794], 'during': [1803, 4087], 'catalysis': [1804, 2012], 'Such': [1824], 'dual': [1826], 'acid-base': [1832], 'catalyst': [1833], 'recently': [1839, 3457], 'questioned': [1841], 'crystallographic': [1843], 'D305A': [1847], 'genuine': [1887, 1913], 'enzyme,': [1893, 4143], 'S-configured': [1896], 'at': [1897, 2275, 2281, 2668, 3006, 3423, 3761, 3795, 3870, 3957, 4005, 4016, 4151, 4243, 4438], 'C2': [1899], 'attached': [1902], 'molecule.': [1904, 3238], 'S': [1906, 1973], 'also': [1909], 'found': [1910], 'using': [1920, 2172, 2193, 2270, 2329, 2457, 2626, 3517, 3636, 3652, 3703, 4131], 'corresponding': [1922], 'D313A.': [1925], 'co-workers': [1928], '(25Alberg': [1929], 'D.G.': [1930], 'Lauhon': [1931], 'Nyfeler': [1933], 'Fassler': [1935], '1992;': [1943], '114:': [1944], '3535-3546Crossref': [1945], '(68)': [1947], '26An': [1950], 'deduced': [1970], 'studies.': [1984], 'stereochemical': [1987], 'course': [1988, 3109, 3770], 'reactions': [1993], 'matter': [1996], 'debate,': [1998], 'partly': [1999], 'lack': [2003], 'understanding': [2005], 'present': [2024, 2031], 'AroA.': [2026], 'work,': [2029], 'evidence': [2032], 'but': [2050, 3265, 3905, 4084], 'fact': [2052], 'crucial': [2056], 'release.': [2059], 'engineered': [2061], 'single-site': [2063], 'carries': [2070], 'instead': [2072, 3328], '115,': [2079], 'MurA:': [2092], 'PEP.': [2095, 2305, 3830], 'Subsequent': [2096], '2.3': [2100, 3001, 4203], 'Å': [2101, 2534, 3002, 4204, 4430, 4437], 'resolution': [2102, 2531, 2631, 2999, 4205], 'surprisingly': [2103], '(EP-UNAG)': [2113], 'confirmed': [2123], 'discuss': [2134], 'implications': [2136], 'catalytic': [2142, 3414], 'possible': [2146], 'associated': [2156], 'catalysis.': [2158], '(potassium': [2160], 'salt)': [2161], 'were': [2164, 2308, 2356, 2517, 2527, 2572, 2642, 2663, 2674, 2726, 2901, 2955, 3193, 3916, 4073, 4144], 'purchased': [2165], 'Sigma.': [2167], 'Protein': [2168, 4380], 'concentration': [2169, 3992], 'Pierce': [2174], 'Coomassie': [2175], 'reagent': [2176, 4108], 'bovine': [2178], 'serum': [2179], 'albumin': [2180], 'standard.': [2182], 'introduced': [2187], 'Kwik': [2195], 'Change': [2196], 'mutagenesis': [2197], 'kit': [2198], 'Stratagene': [2200], 'appropriate': [2202], 'primers.': [2203], 'pET-Vector': [2205], '9d': [2206], '(Novagen)': [2207], 'containing': [2208, 3711, 3940], 'open': [2210, 3272, 3337], 'reading': [2211, 4045], 'frame': [2212], 'point': [2223, 2565], 'C115S-MurA': [2227, 2278, 4182, 4228], 'overexpressed': [2229, 2237], 'STBL2-DE3': [2232], 'purified': [2240, 3616], 'described': [2242], '(21Schonbrunn': [2243], 'Crystallography—The': [2262], 'concentrated': [2266], '∼100': [2268], 'mg/ml': [2269, 3945], 'Centricon': [2271], '30': [2272, 2543], 'devices': [2273], '(Amicon)': [2274], '4': [2276, 2721, 2760, 4006], '°C.': [2277], '19': [2282], '°C': [2283, 3425, 4007, 4018], '10': [2285, 3741, 3765, 3817, 3961, 4020], 'mm': [2286, 2300, 2304, 3713, 3718, 3721, 3724, 3732, 3739, 3782, 3829, 3972], 'pH': [2288, 3715], '6.4,': [2289], '10%': [2290], '(w/v)': [2291, 3860], 'polyethylene': [2292], 'glycol': [2293], '20,000': [2294], '5': [2299, 2303, 3738, 3781, 3828, 3850, 3971, 3979], 'Diffraction': [2306, 2525], 'data': [2307, 2526, 2627, 2709, 2806], 'recorded': [2309, 3794], 'flash': [2313], 'frozen': [2314], 'crystal': [2315, 2587], 'R-axis': [2318], 'IV2+': [2319], 'plate': [2321], 'detector': [2322], '(Molecular': [2323, 2339], 'Structure': [2324, 2340, 2346], 'Corporation,': [2325], 'Woodlands,': [2327], 'TX)': [2328], 'rotation': [2331], 'method': [2332], '(x-rays,': [2333], 'CuKα,': [2334], 'focused': [2335], 'mirror': [2337], 'optics': [2338], 'Corporation);': [2341], 'generator,': [2342], 'RU300': [2343], '(Rigaku,': [2344], 'Molecular': [2345], 'Corporation)).': [2347], 'Data': [2348, 2461, 3275, 3340, 4381], 'processing,': [2349], 'phasing': [2350], 'refinement,': [2352], 'model': [2354, 2667, 3012], 'building': [2355], 'performed': [2357, 2625], 'program': [2360], 'packages': [2361], 'HKL': [2362], '(27Otwinowski': [2364], 'Z.': [2365], 'Minor': [2366], 'Methods': [2368, 2746, 3201], 'Enzymol.': [2369, 2747, 3202], '1997;': [2370, 2748, 2771, 3203], '276:': [2371], '307-326Crossref': [2372], '(38446)': [2375], 'CNS': [2378], '(28Brunger': [2379, 2465, 3279, 3344], 'Adams': [2381, 2467, 3281, 3346], 'P.D.': [2382, 2468, 3282, 3347], 'Clore': [2383, 2469, 3283, 3348], 'DeLano': [2385, 2471, 3285, 3350], 'W.L.': [2386, 2472, 3286, 3351], 'Gros': [2387, 2473, 3287, 3352], 'P.': [2388, 2474, 3288, 3353], 'Grosse-Kunstleve': [2389, 2475, 3289, 3354], 'R.W.': [2390, 2476, 3290, 3355], 'Jiang': [2391, 2477, 3291, 3356], 'Kuszewski': [2393, 2479, 3293, 3358], 'Nilges': [2395, 2481, 3295, 3360], 'Pannu': [2397, 2483, 3297, 3362], 'N.S.': [2398, 2484, 3298, 3363], 'Read': [2399, 2485, 3299, 3364], 'R.J.': [2400, 2486, 3300, 3365], 'Rice': [2401, 2487, 3301, 3366], 'L.M.': [2402, 2488, 3302, 3367], 'Simonson': [2403, 2489, 3303, 3368], 'Warren': [2405, 2491, 3305, 3370], 'G.L.': [2406, 2492, 3306, 3371], 'Acta': [2407, 2433, 2493, 3307, 3372], 'Crystallogr.': [2408, 2412, 2438, 2494, 2498, 2734, 3308, 3312, 3373, 3377], 'Sect.': [2409, 2435, 2495, 3309, 3374], 'B': [2410, 2436, 2496, 3310, 3375], 'Struct.': [2411, 2437, 2497, 3311, 3376], 'Cryst.': [2413, 2439, 2499, 3313, 3378], '54:': [2416, 2502, 3316, 3381], '905-921Crossref': [2417, 2503, 3317, 3382], '(16948)': [2420, 2506, 3320, 3385], 'O': [2424], '(29Jones': [2425], 'T.A.': [2426], 'Zou': [2427], 'J.Y.': [2428], 'Cowan': [2429], 'S.W.': [2430], 'Kjeldgaard': [2431], 'Crys-tallogr.': [2434], '110-119Crossref': [2443], '(13006)': [2446], 'respectively.': [2449, 3251], 'solved': [2453], 'molecular': [2455, 3186], 'replacement,': [2456], '(Protein': [2460, 3274, 3339], 'Bank': [2462, 3276, 3341, 4382], 'code': [2463], '1EJC)': [2464, 3278, 3343], 'search': [2510], 'model.': [2511], 'Solvent': [2512, 2661], 'molecules': [2513, 2612, 2662, 2690, 4229, 4238], 'residues': [2515, 3057], '111-124': [2516], 'omitted': [2518], 'translational': [2521], 'rotational': [2523], 'searches.': [2524], 'limited': [2528], 'low': [2530, 4166], 'reflections': [2532], '20.0-6.0': [2533], 'cross-rotation': [2537, 2548], 'translation': [2539], 'search.': [2540], 'From': [2541], 'highest': [2544, 2630], 'peaks': [2545], 'function,': [2549], 'eight': [2550, 2569, 2610, 2650, 2689, 4225], 'solutions': [2551, 2571, 4123], 'could': [2552, 3924], 'successfully': [2554], 'translated': [2555], 'asymmetric': [2558, 2576, 2583, 2599, 4195], 'unit': [2559, 2584, 4196, 4244], 'crystal.': [2562, 4199, 4241], 'Exploiting': [2563], 'symmetry,': [2567], 'independent': [2570, 4227], 'rearranged': [2573], 'unit.': [2577], 'rearrangement': [2579], 'contains': [2588, 3620], 'tetramers': [2590, 4187], 'molecules.': [2593], 'Rigid': [2594], 'body': [2595], 'refinement': [2596, 2712, 2959], 'octamer,': [2600], 'refined': [2613], 'independently,': [2614], 'lowered': [2615], 'initial': [2617], 'Rfree': [2618, 2945], '50.3': [2620], '36.1%.': [2622], 'Refinement': [2623], 'no': [2633], 'σ': [2634], 'cut-off': [2635], 'applied.': [2636], 'Most': [2637], 'regions': [2641], 'constrained': [2643], 'exploiting': [2644], 'noncrystallographic': [2646], 'symmetry': [2647], 'molecules,': [2651], 'except': [2652], 'intermolecular': [2658], 'contact': [2659], 'regions.': [2660], 'reasonable': [2670], 'positions.': [2671], 'ligands': [2673], 'modeled': [2675, 2692, 3326, 3390], 'according': [2676, 3015], 'clear': [2679], 'electron': [2680, 2976], 'density': [2681, 2977, 2987, 3867], 'map.': [2682], 'Residue': [2683], '67': [2684], 'iso-aspartate': [2694], '(30Eschenburg': [2695], 'Proteins.': [2699, 2795], '40:': [2701], '290-298Crossref': [2702], '(45)': [2705], 'collection': [2710, 2807], 'statistics': [2713], 'summarized': [2715], 'Table': [2717], 'I.': [2718], 'Figs.': [2719], '3,': [2720], '(bottom),': [2722], '7': [2724, 2783], '(a-c)': [2725], 'drawn': [2727, 2762, 2786, 3245], 'Molscript': [2729], '(31Kraulis': [2730], '24:': [2736], '946-950Crossref': [2737], 'Raster3D': [2741], '(32Merrit': [2742, 3197], 'E.A.': [2743, 3198], 'Bacon': [2744, 3199], '277:': [2749, 3204], '505-524Crossref': [2750, 3205], '(3873)': [2753, 3208], 'Fig.': [2759, 2782, 2966], 'Bobscript': [2764], '(33Esnouf': [2765], 'R.M.': [2766], 'Graph.': [2769], 'Model.': [2770], '15:': [2772], '132-134Crossref': [2773], '(1794)': [2776], 'Raster3D,': [2780], '(d-f)': [2784, 3188], 'GRASP': [2788, 3196], '(34Nicholls': [2789], 'Sharp': [2791], 'Honig': [2793], 'B.': [2794], '11:': [2797], '281-296Crossref': [2798], '(5314)': [2801], 'Scholar).Table': [2803], 'ISummary': [2804], 'refinementSpace': [2810], 'groupP21Unit': [2811], 'dimensions': [2813], '(Å)a': [2814], '=': [2815, 2818, 2821, 2824, 2826, 2829, 2860, 2874, 2892, 2915, 2928, 2946], '80.7,': [2816], 'b': [2817, 3260], '153.7,': [2819], 'c': [2820, 3334], '135.1,': [2822], 'α': [2823], 'β': [2825], '90°,': [2827], 'γ': [2828], '104.2°Molecules/asymmetric': [2830], 'unit8Protein': [2831], 'atoms25,144Alternate': [2832], 'positions68Ligand': [2834], 'atoms352Solvent': [2835], 'molecules1816Phosphate': [2836], 'ions9Glycerol': [2837], 'molecules17Rmsd': [2838], 'bonds': [2839], '(Å)aRmsd,': [2840], 'root': [2841, 2907], 'mean': [2842, 2908, 4248, 4264], 'square': [2843, 2909], 'deviation': [2844, 2910], 'values0.007Rmsd': [2847], 'angles': [2848], '(°)1.32Resolution': [2849], 'range': [2850], '(Å)20.0–2.3': [2851], '(2.34–2.30)Measured': [2852], 'reflections755,463': [2853], '(24,806)Unique': [2854], 'reflections140,111': [2855], '(6835)Completeness': [2856], '(%)98.9': [2857], '(97.3)Rsym': [2858], '(%)bRsym': [2859], '100': [2861, 2875, 2916, 2929, 3809, 3856, 4017], '×': [2862, 2876, 2917, 2930], 'ΣhΣI|Ihi': [2863, 2918], '–': [2864, 2878, 2919, 2932], 'Ih|ΣhiIhi,': [2865, 2920], 'h': [2867, 2922], 'represents': [2868, 2923], 'unique': [2869, 2924], 'reflection': [2870, 2925], 'indices10.0': [2871], '(35.0)Rcryst': [2872], '(%)cRcryst': [2873], 'Σ|Fobs': [2877, 2931], 'Fmodel|/ΣFobs,': [2879, 2933], 'Fobs': [2881, 2935], 'Fmodel': [2883, 2937], 'observed': [2885, 2939, 3257, 4289], 'calculated': [2887, 2894, 2941, 2948, 3194], 'factor': [2889, 2943, 4250, 4266], 'amplitudes17.8Rfree': [2890], '(%)dRfree': [2891], 'Rcryst': [2893, 2927, 2947], '2%': [2896, 2950], 'randomly': [2897, 2951], 'chosen': [2898, 2952], 'reflections,': [2899, 2953], 'excluded': [2902, 2956], 'refinement22.5a': [2905], 'Rmsd,': [2906], 'valuesb': [2913], 'Rsym': [2914], 'indicesc': [2926], 'amplitudesd': [2944], 'Open': [2960], 'table': [2961], 'tab': [2965], '4Interaction': [2967], 'its': [2971], 'products.': [2972], 'Top': [2973], 'stereo': [2974, 3045], 'pair,': [2975, 3046], 'derived': [2989], '2Fo': [2992], '-': [2993], '1Fc': [2994], 'Fourier': [2995], 'contoured': [3005], '1': [3007, 3757], 'σ.': [3008], 'overlaid': [3010], 'color-coded': [3014], 'types:': [3018], 'carbon': [3019], 'atoms': [3020, 3029, 3034, 3040, 4253, 4418], 'green,': [3027, 3162, 3250], 'nitrogen': [3028], 'blue,': [3032], 'oxygen': [3033, 4465], 'red,': [3037], 'phosphorus': [3039], 'magenta.': [3043], 'Bottom': [3044], 'green;': [3053], 'surrounding': [3055], 'gray': [3060, 3231], '(hydrophobic)': [3061], 'pale': [3063], 'violet': [3064], '(polar/charged);': [3065], 'solvent': [3067], 'structure,': [3068], 'including': [3069], 'glycerol': [3071], 'molecule': [3072], 'cryo': [3075], 'buffer,': [3076], 'transparent': [3080, 3214, 3221, 3230], 'cyan.': [3081], 'Dashed': [3082], 'lines': [3083], 'represent': [3084], 'polar': [3085], 'charged': [3087], 'interactions.': [3088], 'EP-UNAG': [3093, 3159, 3243, 3388, 4130, 4213], 'Pro112-Pro121.View': [3098], '(PPT)Fig.': [3106], '7Proposed': [3107], 'structural': [3108], 'approximately': [3118], 'along': [3119], 'presumable': [3121], 'exit': [3122], 'route': [3123], 'ion.': [3127], 'a-c,': [3128], 'schematic': [3129], 'representation': [3130], 'Pro112-Pro121': [3139, 3218], 'turquoise,': [3143], 'loop-anchoring': [3145], 'elements': [3147], '(β-strand': [3148], 'b2': [3149], 'helix': [3151], 'a2': [3152], 'subdomain': [3154], 'IIc)': [3155], 'yellow,': [3158], 'orange.': [3169], 'Residues': [3170], 'presumably': [3176], 'ball-and-stick': [3183], 'forms.': [3184], 'surfaces': [3187], 'structures': [3191], 'a-c': [3192], 'displayed': [3212], 'turquoise': [3215], 'region,': [3220], 'blue': [3222], 'Arg397,': [3228], 'rest': [3234], 'solid': [3247], 'red': [3248], 'd,': [3254], 'MurA:P1P2.': [3259], 'e,': [3262], 'above,': [3264], 'Arg397': [3267], 'oriented': [3268], 'unliganded,': [3271], 'entry': [3277, 3342, 4383], '115.': [3333], 'f,': [3336], '(transparent': [3391], 'green)': [3392], 'relative': [3397], 'bottom': [3400], 'above.View': [3403], 'Analysis—The': [3413], 'tested': [3422], '20': [3424, 3723, 3726], 'Shimadzu': [3428], '1650PC': [3429], 'spectrophotometer.': [3430], 'To': [3431, 3522, 3698, 3885, 3981, 4022], 'measure': [3432], 'activity,': [3435], 'made': [3437], 'use': [3438], 'assays;': [3441], 'one': [3442, 3518], 'uses': [3443], 'MurB': [3444, 3460, 3705], 'coupling': [3447], 'detect': [3450, 3559], 'enolpyruvyl-UNAG': [3453], '(EP-UNAG).': [3454], 'coupled': [3461, 3497, 3706, 4029], 'assay': [3462, 3588, 3801, 3814, 4030], 'reevaluation': [3465], 'mode': [3468], '(17Schonbrunn': [3477], 'starts': [3499], 'converting': [3502], 'UNAG-EP,': [3507, 4027], 'reduced': [3511], 'UDP-N-acetylmuramic': [3513], 'acid': [3514, 4000, 4170], 'MurB,': [3516, 3529, 3737, 4062], 'equivalent': [3519, 4412], 'NADPH.': [3521], 'compensate': [3523], 'diaphorase': [3526], 'glucose': [3531, 3729], 'oxidase/glucose': [3532], 'system': [3533], 'exploited.': [3535], 'resulted': [3537], 'stable': [3540, 4044], 'base': [3541], 'line': [3542], 'prior': [3543, 3776, 4048], 'initiation': [3545], 'assay,': [3552, 3555, 3707], 'Lanzetta': [3554, 3587, 3800, 3843, 4107, 4174], '(35Lanzetta': [3564], 'Alvarez': [3566], 'L.J.': [3567], 'Reinach': [3568], 'P.S.': [3569], 'Candia': [3570], 'O.A.': [3571], 'Anal.': [3572], '1979;': [3574], '100:': [3575], '95-97Crossref': [3576], '(1810)': [3579], 'previously': [3584, 4290], 'utilized': [3585], 'characterize': [3590], 'synthase': [3595], '(36Eschenburg': [3596], 'Priestman': [3600], 'M.A.': [3601], 'Lushington': [3602], 'G.H.': [3603], 'Planta.': [3606], '216:': [3608], '129-135Crossref': [3609], '(95)': [3612], 'trace': [3621], 'amounts': [3622], 'genomic': [3624], 'interfere': [3630], 'kinetic': [3633], 'analysis,': [3634], 'especially': [3635], 'concentrations': [3639, 4033], 'here.': [3644], 'Therefore': [3645], 'selectively': [3647], 'inactivated': [3648], 'Inhibition': [3654], 'solely': [3660], 'due': [3661], 'attachment': [3664], 'Cys115,': [3666], 'affected': [3674], 'test': [3699, 3919, 4023], 'mixture': [3710, 3815, 3939, 4037], '50': [3712, 3717], 'Tris,': [3714], '8.0,': [3716], 'KCl,': [3719], '2': [3720], 'dithiothreitol,': [3722], 'glucose,': [3725], 'units': [3727], 'oxidase,': [3730], '0.20': [3731], 'NADPH,': [3733], '40': [3734, 3944, 4059], 'μg': [3735, 4060], 'μm': [3742], '1.8': [3745], 'mg': [3746], '(0.04': [3750], 'mm),': [3751], 'final': [3754, 3952, 3991], 'volume': [3755, 3953], 'ml,': [3758], 'incubated': [3760, 3807, 4004], 'room': [3762, 3958], 'temperature': [3763, 3959, 4249, 4265], 'min.': [3766, 3962, 3980, 4021], 'base-line': [3768], 'time': [3769], 'run': [3772], '∼30': [3774], 's': [3775], 'start': [3785, 4051], 'decrease': [3789, 4065], 'NADPH': [3791, 4067], 'absorbance': [3792, 4068], '340': [3796], 'nm.': [3797], '(0.05': [3804], 'mm)': [3805, 3949, 4040], 'μl': [3810, 3840, 3857, 3956], 'min': [3818, 3851], 'initiated': [3823, 3966], 'stopped': [3834, 3848], '800': [3839], 'reagent.': [3844, 4175], 'Color': [3845], 'development': [3846], 'after': [3849, 3927], '34%': [3859], 'sodium': [3861], 'citrate.': [3862], 'change': [3864], 'optical': [3866], 'measured': [3869], '660': [3871], 'nm,': [3872], 'amount': [3875, 4093], 'standards.': [3884], 'determine': [3886], 'whether': [3887, 3920], 'capable': [3892], 'catalyzing': [3894], 'conversion': [3896], 'UNAG-EP': [3902], 'unable': [3907], '(single': [3912], 'turnover),': [3913], 'several': [3914], 'denaturants': [3915], 'detected': [3926], 'denaturation': [3928, 4036], 'enzyme-ligand': [3931], 'complex.': [3932], 'accomplished': [3935], 'incubating': [3937], 'buffer': [3942], '(0.9': [3948], '400': [3955], 'allowed': [3975], 'stop': [3982], 'denature': [3986, 4138], '6': [3994], 'm': [3995], 'urea': [3996, 4122], '5%': [3998], 'trichloroacetic': [3999, 4135, 4169], '16': [4009], 'h.': [4010], 'Alternatively,': [4011], 'heated': [4015], 'varied': [4032], '(0.4-2.9': [4038], 'mg/0.01-0.07': [4039], 'employed.': [4042], 'established': [4047], 'recorded.': [4070], 'Control': [4071], 'experiments': [4072], 'conducted': [4074], 'throughout': [4075], 'treated': [4079], 'way': [4083], 'omitting': [4085], 'time.': [4090], 'Testing': [4091], 'produced': [4096], 'failed': [4100], 'noncompatibility': [4104], 'concentrations.': [4112], 'Notably,': [4113], 'precipitate': [4119, 4140], 'out': [4120], 'cannot': [4126], 'separated': [4128], 'centrifugation.': [4132], 'Heat': [4133], 'acid,': [4136], 'suitable;': [4146], 'nonreacting': [4147], 'hydrolyzes': [4149], 'rapidly': [4150], 'elevated': [4152], 'temperatures': [4153], 'Pi,': [4157, 4215], 'thereby': [4158], 'producing': [4159], 'too': [4160], 'background': [4162], 'levels,': [4163], '0.2%': [4168], 'interferes': [4171], 'Enzyme-Products': [4176], 'Complex—Under': [4177], 'crystallization': [4179], 'conditions': [4180], 'used,': [4181], 'oligomerizes': [4183], 'densely': [4185], 'packed': [4186], '3),': [4189], 'constitute': [4193], 'X-ray': [4200], 'crystallographically': [4226], '4).': [4231], 'uniformly': [4234], 'occupy': [4235], 'all': [4236], 'Refined': [4242], 'occupancy,': [4245], 'lies': [4257], '17.0': [4259], 'Å2': [4260], 'yet': [4261], 'below': [4262], '23.9': [4271], 'Å2.': [4272], 'liganded': [4274], 'C115S-MurA,': [4275], 'following': [4279], 'denoted': [4281], 'MurA:P1P2,': [4283], 'exhibits': [4284], 'closed': [4286], 'conformation': [4288], '24Skarzynski': [4343], 'Its': [4362], 'overall': [4363], 'fold': [4364], 'virtually': [4366], 'identical': [4367], '(MurA:THI;': [4379], '1Q3G)': [4384], 'positions': [4410], 'pairs': [4413], '419': [4416], 'Cα': [4417], 'MurA:P1P2': [4420, 4451], 'MurA:THI': [4423, 4449], 'deviate': [4424], 'average': [4426], '0.23': [4429], 'maximum': [4433], 'discrepancy': [4434], '1.7': [4436], 'C': [4440], 'terminus': [4441], 'chain.': [4445], 'Detailed': [4446], 'suggests': [4452], 'cleaves': [4456], 'enol': [4464], 'without': [4471], 'macroscopic': [4472], 'changes': [4473], 'active-si': [4476]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2070243130', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 11}, {'year': 2022, 'cited_by_count': 8}, {'year': 2021, 'cited_by_count': 7}, {'year': 2020, 'cited_by_count': 6}, {'year': 2019, 'cited_by_count': 8}, {'year': 2018, 'cited_by_count': 10}, {'year': 2017, 'cited_by_count': 8}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 5}, {'year': 2014, 'cited_by_count': 4}, {'year': 2013, 'cited_by_count': 9}, {'year': 2012, 'cited_by_count': 10}], 'updated_date': '2024-12-25T07:54:35.707670', 'created_date': '2016-06-24'}