Surface plasmon resonance for real-time monitoring of molecular interactions between a triple helix forming oligonucleotide and the Sp1 binding sites of human Ha-ras promoter: effects of the DNA-binding drug chromomycin.

Name: Work Video:
Duration: 3 min 30 s
Cristina Rutigliano; Nicoletta Bianchi; Marina Tomassetti; Lara Pippo; Carlo Mischiati; Giordana Feriotto; Roberto Gambari
{'id': 'https://openalex.org/W2069878118', 'doi': 'https://doi.org/10.3892/ijo.12.2.337', 'title': 'Surface plasmon resonance for real-time monitoring of molecular interactions between a triple helix forming oligonucleotide and the Sp1 binding sites of human Ha-ras promoter: effects of the DNA-binding drug chromomycin.', 'display_name': 'Surface plasmon resonance for real-time monitoring of molecular interactions between a triple helix forming oligonucleotide and the Sp1 binding sites of human Ha-ras promoter: effects of the DNA-binding drug chromomycin.', 'publication_year': 1998, 'publication_date': '1998-02-01', 'ids': {'openalex': 'https://openalex.org/W2069878118', 'doi': 'https://doi.org/10.3892/ijo.12.2.337', 'mag': '2069878118', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/9458359'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.3892/ijo.12.2.337', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S169822654', 'display_name': 'International Journal of Oncology', 'issn_l': '1019-6439', 'issn': ['1019-6439'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320412', 'host_organization_name': 'Spandidos Publishing', 'host_organization_lineage': ['https://openalex.org/P4310320412'], 'host_organization_lineage_names': ['Spandidos Publishing'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5017285713', 'display_name': 'Cristina Rutigliano', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I201324441', 'display_name': 'University of Ferrara', 'ror': 'https://ror.org/041zkgm14', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I201324441']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'C Rutigliano', 'raw_affiliation_strings': ['Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy', 'institution_ids': ['https://openalex.org/I201324441']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5013472145', 'display_name': 'Nicoletta Bianchi', 'orcid': 'https://orcid.org/0000-0001-9280-6017'}, 'institutions': [{'id': 'https://openalex.org/I201324441', 'display_name': 'University of Ferrara', 'ror': 'https://ror.org/041zkgm14', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I201324441']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'N Bianchi', 'raw_affiliation_strings': ['Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy', 'institution_ids': ['https://openalex.org/I201324441']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5049498723', 'display_name': 'Marina Tomassetti', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I201324441', 'display_name': 'University of Ferrara', 'ror': 'https://ror.org/041zkgm14', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I201324441']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'M Tomassetti', 'raw_affiliation_strings': ['Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy', 'institution_ids': ['https://openalex.org/I201324441']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5035051359', 'display_name': 'Lara Pippo', 'orcid': 'https://orcid.org/0000-0001-6414-3500'}, 'institutions': [{'id': 'https://openalex.org/I201324441', 'display_name': 'University of Ferrara', 'ror': 'https://ror.org/041zkgm14', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I201324441']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'L Pippo', 'raw_affiliation_strings': ['Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy', 'institution_ids': ['https://openalex.org/I201324441']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5067299527', 'display_name': 'Carlo Mischiati', 'orcid': 'https://orcid.org/0000-0002-0029-2205'}, 'institutions': [{'id': 'https://openalex.org/I201324441', 'display_name': 'University of Ferrara', 'ror': 'https://ror.org/041zkgm14', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I201324441']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'C Mischiati', 'raw_affiliation_strings': ['Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy', 'institution_ids': ['https://openalex.org/I201324441']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5038260515', 'display_name': 'Giordana Feriotto', 'orcid': 'https://orcid.org/0000-0001-9840-3948'}, 'institutions': [{'id': 'https://openalex.org/I201324441', 'display_name': 'University of Ferrara', 'ror': 'https://ror.org/041zkgm14', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I201324441']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'G Feriotto', 'raw_affiliation_strings': ['Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy', 'institution_ids': ['https://openalex.org/I201324441']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5070584148', 'display_name': 'Roberto Gambari', 'orcid': 'https://orcid.org/0000-0001-9205-6033'}, 'institutions': [{'id': 'https://openalex.org/I201324441', 'display_name': 'University of Ferrara', 'ror': 'https://ror.org/041zkgm14', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I201324441']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'R Gambari', 'raw_affiliation_strings': ['Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry and Molecular Biology; University of Ferrara; Ferrara, Italy', 'institution_ids': ['https://openalex.org/I201324441']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.575, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 11, 'citation_normalized_percentile': {'value': 0.536775, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 79, 'max': 80}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10432', 'display_name': 'DNA and Nucleic Acid Chemistry', 'score': 0.9637, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10432', 'display_name': 'DNA and Nucleic Acid Chemistry', 'score': 0.9637, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/dna-binding-site', 'display_name': 'DNA binding site', 'score': 0.45055723}, {'id': 'https://openalex.org/keywords/hmg-box', 'display_name': 'HMG-box', 'score': 0.44209236}, {'id': 'https://openalex.org/keywords/transcription', 'display_name': 'Transcription', 'score': 0.4319202}], 'concepts': [{'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.70639163}, {'id': 'https://openalex.org/C129312508', 'wikidata': 'https://www.wikidata.org/wiki/Q133686', 'display_name': 'Oligonucleotide', 'level': 3, 'score': 0.70258284}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.67971104}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.59687376}, {'id': 'https://openalex.org/C107824862', 'wikidata': 'https://www.wikidata.org/wiki/Q616005', 'display_name': 'Binding site', 'level': 2, 'score': 0.55947524}, {'id': 'https://openalex.org/C101762097', 'wikidata': 'https://www.wikidata.org/wiki/Q224093', 'display_name': 'Promoter', 'level': 4, 'score': 0.5040585}, {'id': 'https://openalex.org/C86339819', 'wikidata': 'https://www.wikidata.org/wiki/Q407384', 'display_name': 'Transcription factor', 'level': 3, 'score': 0.45405132}, {'id': 'https://openalex.org/C106847996', 'wikidata': 'https://www.wikidata.org/wiki/Q898756', 'display_name': 'Surface plasmon resonance', 'level': 3, 'score': 0.45319167}, {'id': 'https://openalex.org/C3662595', 'wikidata': 'https://www.wikidata.org/wiki/Q5205743', 'display_name': 'DNA binding site', 'level': 5, 'score': 0.45055723}, {'id': 'https://openalex.org/C5179208', 'wikidata': 'https://www.wikidata.org/wiki/Q3782057', 'display_name': 'HMG-box', 'level': 5, 'score': 0.44209236}, {'id': 'https://openalex.org/C179926584', 'wikidata': 'https://www.wikidata.org/wiki/Q207714', 'display_name': 'Transcription (linguistics)', 'level': 2, 'score': 0.4319202}, {'id': 'https://openalex.org/C94966510', 'wikidata': 'https://www.wikidata.org/wiki/Q2252764', 'display_name': 'DNA-binding protein', 'level': 4, 'score': 0.34832188}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.28706956}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.2311463}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.12589797}, {'id': 'https://openalex.org/C192562407', 'wikidata': 'https://www.wikidata.org/wiki/Q228736', 'display_name': 'Materials science', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C41895202', 'wikidata': 'https://www.wikidata.org/wiki/Q8162', 'display_name': 'Linguistics', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C138885662', 'wikidata': 'https://www.wikidata.org/wiki/Q5891', 'display_name': 'Philosophy', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C155672457', 'wikidata': 'https://www.wikidata.org/wiki/Q61231', 'display_name': 'Nanoparticle', 'level': 2, 'score': 0.0}, {'id': 'https://openalex.org/C171250308', 'wikidata': 'https://www.wikidata.org/wiki/Q11468', 'display_name': 'Nanotechnology', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D002865', 'descriptor_name': 'Chromomycins', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': True}, {'descriptor_ui': 'D004268', 'descriptor_name': 'DNA-Binding Proteins', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': True}, {'descriptor_ui': 'D011905', 'descriptor_name': 'Genes, ras', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': True}, {'descriptor_ui': 'D009841', 'descriptor_name': 'Oligonucleotides', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D016329', 'descriptor_name': 'Sp1 Transcription Factor', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': True}, {'descriptor_ui': 'D020349', 'descriptor_name': 'Surface Plasmon Resonance', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002273', 'descriptor_name': 'Carcinogens', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002865', 'descriptor_name': 'Chromomycins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004268', 'descriptor_name': 'DNA-Binding Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011905', 'descriptor_name': 'Genes, ras', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D066298', 'descriptor_name': 'In Vitro Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008969', 'descriptor_name': 'Molecular Sequence Data', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009841', 'descriptor_name': 'Oligonucleotides', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011401', 'descriptor_name': 'Promoter Regions, Genetic', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016329', 'descriptor_name': 'Sp1 Transcription Factor', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016329', 'descriptor_name': 'Sp1 Transcription Factor', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D020349', 'descriptor_name': 'Surface Plasmon Resonance', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013997', 'descriptor_name': 'Time Factors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.3892/ijo.12.2.337', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S169822654', 'display_name': 'International Journal of Oncology', 'issn_l': '1019-6439', 'issn': ['1019-6439'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320412', 'host_organization_name': 'Spandidos Publishing', 'host_organization_lineage': ['https://openalex.org/P4310320412'], 'host_organization_lineage_names': ['Spandidos Publishing'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/9458359', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W2586219588', 'https://openalex.org/W2148072770', 'https://openalex.org/W2099901107', 'https://openalex.org/W2052894840', 'https://openalex.org/W2030922238', 'https://openalex.org/W2028056900', 'https://openalex.org/W1974642429', 'https://openalex.org/W1926921733', 'https://openalex.org/W1870851597', 'https://openalex.org/W1776902257'], 'abstract_inverted_index': {'DNA-binding': [0, 20, 79, 143], 'molecules': [1], 'have': [2], 'been': [3], 'recently': [4], 'proposed': [5], 'as': [6], 'potential': [7], 'inhibitors': [8], 'of': [9, 27, 32, 42, 69, 77, 102, 114, 121, 125, 134, 141, 158, 163], 'molecular': [10, 40, 58, 103, 147, 169], 'interactions': [11, 59, 148, 170], 'between': [12, 60, 105, 149, 171], 'transcription': [13, 174], 'factors': [14], 'and': [15, 64, 91, 98, 108, 152, 176], 'target': [16, 65, 109, 131, 153, 177], 'DNA': [17, 132, 178], 'sequences.': [18, 179], 'Among': [19], 'drugs,': [21], 'chromomycin': [22, 107, 122, 159], 'binds': [23], 'to': [24, 51, 129], 'GC-rich': [25], 'sequences': [26, 133], 'the': [28, 33, 70, 75, 78, 126, 135, 150, 161, 164, 168], 'Sp1': [29, 66, 130, 173], 'binding': [30, 67, 124], 'sites': [31, 37, 68], 'Ha-ras': [34, 53, 72, 136], 'oncogene.': [35], 'These': [36], 'are': [38], 'also': [39], 'targets': [41], 'a': [43], 'triple-helix': [44, 61], 'forming': [45, 62], 'oligonucleotide': [46, 128], '[Sp1(Ha-ras)TFO]': [47], 'which': [48], 'is': [49, 117], 'able': [50], 'inhibit': [52], 'oncogene': [54, 137], 'transcription.': [55], 'We': [56], 'studied': [57], 'oligonucleotides': [63], 'human': [71], 'promoter': [73], 'in': [74, 166], 'presence': [76], 'drug': [80, 144], 'chromomycin.': [81], 'This': [82], 'study': [83, 116], 'was': [84], 'performed': [85], 'by': [86], '(a)': [87], 'surface': [88], 'plasmon': [89], 'resonance': [90], 'biosensor': [92], 'technology,': [93], '(b)': [94], 'gel': [95], 'retardation': [96], 'assay': [97], '(c)': [99], 'magnetic': [100], 'capturing': [101], 'complexes': [104], 'TFO,': [106], 'DNA.': [110, 154], 'The': [111], 'main': [112], 'conclusion': [113], 'our': [115], 'that': [118], 'low': [119, 156], 'concentrations': [120, 140, 157], 'allow': [123], 'triplex-forming': [127], 'promoter.': [138], 'Higher': [139], 'this': [142], 'fully': [145], 'suppress': [146], 'Sp1(Ha-ras)TFO': [151, 165], 'Additionally,': [155], 'potentiate': [160], 'effects': [162], 'inhibiting': [167], 'purified': [172], 'factor': [175]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2069878118', 'counts_by_year': [], 'updated_date': '2024-12-08T20:36:19.728362', 'created_date': '2016-06-24'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
