Title: Effect of protein disulfide isomerase on the regeneration of bovine ribonuclease A with dithiothreitol
Abstract:The role of protein disulfide isomerase (PDI) in the regeneration of ribonuclease A with dithiothreitol (DTT) was investigated at three different temperatures. The rates of formation of the native pro...The role of protein disulfide isomerase (PDI) in the regeneration of ribonuclease A with dithiothreitol (DTT) was investigated at three different temperatures. The rates of formation of the native protein were markedly increased in the presence of PDI, 9‐fold at 15°C, 6‐fold at 25°C and 62‐fold at 37°C, respectively. In the presence of PDI, major changes were found in the distribution of intermediates in the three‐disulfide region at 25 and 15°C and also in the one‐disulfide region at 15°C, with the fast accumulation of the two native‐like species des‐[65‐72] and des‐[40‐95]. The present results indicate that PDI does not alter the two major parallel pathways involving des‐[65‐72] and des‐[40‐95] in the regeneration of ribonuclease A with DTT.Read More
Publication Year: 1999
Publication Date: 1999-07-28
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 10
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