Publication Information

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2535, 3420, 3755, 3799, 3935, 3964], 'in': [7, 27, 53, 85, 87, 91, 95, 98, 117, 146, 159, 171, 194, 267, 287, 313, 345, 347, 351, 355, 358, 377, 406, 419, 431, 454, 523, 527, 563, 582, 618, 629, 827, 882, 1047, 1114, 1230, 1269, 1277, 1313, 1364, 1393, 1398, 1419, 1473, 1494, 1536, 1592, 1889, 1920, 1987, 1994, 2015, 2028, 2262, 2275, 2281, 2317, 2335, 2347, 2371, 2389, 2400, 2432, 2451, 2453, 2473, 2527, 2641, 2714, 2747, 2767, 2837, 2871, 2893, 2927, 2973, 2979, 3001, 3051, 3108, 3129, 3230, 3276, 3350, 3473, 3535, 4075, 4084, 4151, 4166, 4365], 'cells': [8, 153, 247, 268, 413, 507, 524, 1290, 1327, 1366, 2332, 2362, 2402, 3126], 'are': [9, 269, 560, 721, 863, 1200, 1262, 1481, 1853, 2074, 2097, 2232, 2730, 2751, 2854, 2876, 3406, 3408, 3519, 3651, 3699], 'labor-intensive': [10, 270], 'and': [11, 75, 78, 81, 97, 105, 112, 125, 179, 184, 232, 271, 335, 338, 341, 357, 365, 372, 385, 439, 444, 492, 543, 593, 790, 822, 865, 946, 990, 1033, 1058, 1142, 1185, 1274, 1397, 1643, 1661, 1858, 1866, 2004, 2021, 2036, 2057, 2100, 2173, 2220, 2249, 2298, 2312, 2354, 2367, 2479, 2502, 2584, 2646, 2649, 2654, 2656, 2659, 2677, 2697, 2700, 2708, 2725, 2755, 2773, 2808, 2813, 2818, 2823, 2828, 2833, 2848, 2880, 2900, 2922, 2933, 2988, 2991, 3022, 3048, 3094, 3099, 3140, 3149, 3190, 3204, 3214, 3242, 3250, 3261, 3272, 3344, 3373, 3439, 3458, 3492, 3513, 3524, 3543, 3581, 3635, 3648, 3657, 3695, 3697, 3708, 3732, 3761, 3810, 3870, 3884, 3957, 3978, 4070, 4095, 4105, 4130, 4157, 4187, 4202, 4213, 4247, 4282, 4304, 4362], 'generally': [12, 272], 'require': [13, 273, 1326], 'destruction': [14, 274], 'of': [15, 25, 40, 103, 108, 245, 275, 285, 300, 363, 368, 505, 548, 595, 615, 626, 744, 817, 824, 858, 936, 942, 1030, 1036, 1101, 1121, 1213, 1235, 1245, 1253, 1273, 1302, 1311, 1349, 1430, 1444, 1476, 1486, 1491, 1505, 1640, 1655, 1658, 1851, 1864, 1896, 1909, 1924, 1956, 1961, 2009, 2018, 2046, 2065, 2071, 2087, 2171, 2223, 2227, 2245, 2255, 2265, 2295, 2302, 2307, 2351, 2385, 2397, 2410, 2424, 2442, 2507, 2534, 2543, 2588, 2638, 2684, 2712, 2788, 2803, 2916, 2952, 2969, 3006, 3010, 3018, 3027, 3045, 3079, 3115, 3157, 3226, 3248, 3308, 3415, 3435, 3446, 3456, 3468, 3487, 3508, 3538, 3541, 3555, 3622, 3626, 3633, 3655, 3665, 3675, 3684, 3704, 3756, 3800, 3848, 3896, 3923, 3932, 3942, 3965, 4011, 4078, 4120, 4126, 4136, 4210, 4216, 4231], 'tissue.': [16, 276], 'This': [17, 277, 536, 586, 1382, 4013], 'precludes': [18, 278], 'single': [19, 279, 1524, 1891, 2361, 4262], 'cell': [20, 160, 196, 280, 420, 456, 571, 1059, 1440, 1892, 2251, 2369], 'or': [21, 35, 158, 281, 295, 418, 534, 1233, 2238, 2457, 2675, 3463, 4060, 4103, 4195, 4252], 'real': [22, 282], 'time': [23, 283], 'studies': [24, 284], 'changes': [26, 84, 90, 130, 286, 344, 350, 390, 1287, 1436, 1493, 1535, 2027, 2244, 2346, 2452, 3472], 'poise': [29, 289, 1421], 'that': [30, 131, 166, 190, 242, 290, 391, 426, 450, 502, 574, 724, 1207, 1530, 1651, 2162, 2321, 2340, 2393, 2420, 3071, 3405, 3430, 3584], 'result': [31, 291, 1229, 2450], 'from': [32, 292, 1389, 1408, 1543, 1905, 2080, 2159, 2243, 3568, 3770, 3939, 3974, 4088, 4260], 'metabolic': [33, 293, 521], 'processes': [34, 294, 1020, 1227], 'environmental': [36, 296], 'influences.': [37, 297], 'By': [38, 298, 1489, 3725], 'substitution': [39, 299, 2254, 2506], 'surface-exposed': [41, 301, 2425], 'residues': [42, 302, 1238, 2257, 2427], 'on': [43, 151, 303, 411, 1239, 2360, 2669, 3211, 3927, 4001, 4008, 4063], 'the': [44, 118, 135, 144, 167, 175, 191, 243, 257, 304, 378, 395, 404, 427, 435, 451, 503, 517, 532, 567, 579, 590, 610, 624, 630, 736, 745, 820, 883, 933, 1006, 1049, 1102, 1115, 1211, 1231, 1251, 1271, 1347, 1359, 1394, 1438, 1477, 1484, 1495, 1502, 1506, 1544, 1878, 1913, 1921, 1925, 1930, 1959, 2007, 2016, 2047, 2053, 2062, 2066, 2069, 2085, 2095, 2160, 2217, 2263, 2266, 2282, 2296, 2318, 2325, 2336, 2341, 2381, 2386, 2388, 2394, 2418, 2433, 2439, 2443, 2454, 2469, 2519, 2525, 2544, 2581, 2685, 2690, 2702, 2719, 2733, 2762, 2842, 2857, 2888, 2939, 2947, 2953, 2974, 2982, 2996, 3003, 3007, 3034, 3043, 3077, 3081, 3086, 3116, 3150, 3257, 3295, 3309, 3313, 3317, 3333, 3351, 3428, 3431, 3443, 3447, 3474, 3488, 3506, 3514, 3536, 3552, 3565, 3569, 3572, 3576, 3623, 3627, 3630, 3652, 3662, 3672, 3690, 3700, 3705, 3729, 3734, 3744, 3747, 3771, 3796, 3801, 3830, 3839, 3846, 3873, 3894, 3921, 3933, 3940, 3952, 3962, 3975, 4002, 4009, 4079, 4085, 4089, 4118, 4121, 4127, 4137, 4200, 4273, 4339, 4350, 4368], 'Aequorea': [45, 305], 'victoria': [46, 306], 'green': [47, 307, 1545, 1554, 1571], 'fluorescent': [48, 308, 1546, 1555, 1564, 1572, 1581, 1931, 1963, 2516], 'protein': [49, 309, 884, 891, 1242, 1267, 1547, 1644, 1964, 3105, 3574, 4064, 4211], '(GFP)': [50, 310], 'with': [51, 174, 201, 311, 434, 461, 890, 1356, 1424, 2277, 2368, 2375, 2417, 2579, 2699, 3120, 3172, 3192, 3264, 3478, 3490, 3889, 4284, 4308, 4318], 'cysteines': [52, 312, 1957, 2471], 'appropriate': [54, 314, 1988], 'positions': [55, 315, 2477], 'to': [56, 89, 143, 181, 316, 349, 403, 441, 729, 869, 1054, 1109, 1119, 1203, 1223, 1243, 1265, 1328, 1344, 1351, 1387, 1391, 1406, 1410, 1416, 1433, 1500, 1508, 1534, 1874, 1886, 1990, 2024, 2031, 2043, 2104, 2285, 2349, 2438, 2449, 2467, 2510, 2513, 3041, 3154, 3274, 3305, 3386, 3396, 3571, 3595, 3640, 3736, 3741, 4017, 4123, 4147, 4359, 4367], 'form': [57, 317, 1991, 3586], 'disulfide': [58, 124, 318, 384, 558, 603, 627, 732, 737, 825, 832, 1992, 2092, 2310, 2536, 2590, 2692, 3589, 4193], 'bonds,': [59, 319, 3590], 'reduction-oxidation-sensitive': [60, 320], 'GFPs': [61, 321, 2293], '(roGFPs)': [62, 322], 'have': [63, 67, 323, 327, 1044, 1106, 1317, 1321, 1341, 1648], 'been': [64, 141, 324, 401, 607, 878, 1045, 1107, 1306, 1318, 1342, 1385, 1404, 1649], 'created.': [65, 325], 'roGFPs': [66, 326, 3423, 3436, 3469, 3489, 3532, 3585, 3757], 'two': [68, 328, 2470, 2636, 2720, 2789, 2804, 2843, 2917, 3475, 3714, 3993], 'fluorescence': [69, 86, 156, 329, 346, 416, 2029, 2088, 2164, 2365, 2455, 3219, 3432, 3466, 3681, 3994], 'excitation': [70, 330, 2456, 2706, 2768, 2894, 3207, 3433, 3476, 3685, 3995, 4080, 4090, 4128], 'maxima': [71, 331, 3477, 3996, 4081], 'at': [72, 110, 212, 332, 370, 472, 1883, 1915, 2258, 2476, 2771, 2806, 2811, 2816, 2821, 2826, 2831, 2898, 2920, 3179, 3221, 3291, 3299, 3558, 3668, 3686, 3758, 3807, 3951, 3967, 3980, 4092, 4163, 4269, 4272], 'about': [73, 333], '400': [74, 334, 2774, 2809, 2814, 2819, 2824, 2829, 2834, 2901, 2923, 4094, 4104], '490': [76, 336, 2812, 2822, 2832, 2921, 3687, 4106], 'nm': [77, 337, 3688, 3702, 4097, 4107], 'display': [79, 339, 3992], 'rapid': [80, 340], 'reversible': [82, 342, 2305], 'ratiometric': [83, 343, 2099, 2233, 4019], 'response': [88, 348, 1533, 2348], 'vitro': [96, 356, 4076], 'vivo.': [99, 359], 'Crystal': [100, 360], 'structure': [101, 361], 'analyses': [102, 362], 'reduced': [104, 364, 1350, 2299, 3251, 3343, 3457, 3491, 3509, 3556, 3573, 3666, 3709, 4203, 4283], 'oxidized': [106, 119, 366, 379, 1352, 2297, 3249, 3345, 3459, 3493, 3570, 3676, 3707, 4201], 'crystals': [107, 367, 1475, 4161, 4225], 'roGFP2': [109, 369, 2303, 3093], '2.0-': [111, 371], '1.9-Å': [113, 373], 'resolution,': [114, 374], 'respectively,': [115, 375, 3659, 3711], 'reveal': [116, 165, 376, 425], 'state': [120, 380, 1923, 2064, 3445, 4010, 4268], 'a': [121, 155, 163, 202, 381, 415, 423, 462, 731, 814, 1034, 1111, 1240, 1281, 1442, 1586, 1637, 1861, 1890, 1894, 1918, 2168, 2364, 2589, 2928, 2966, 3155, 3277, 3281, 3479, 3500, 3680, 3737, 4134, 4229, 4261], 'highly': [122, 199, 382, 459, 830, 1292], 'strained': [123, 383], 'localized': [126, 386, 1884], 'main': [127, 387], 'chain': [128, 388], 'structural': [129, 389, 2430], 'presumably': [132, 392, 538], 'account': [133, 393, 2323], 'state-dependent': [136, 396], 'spectral': [137, 397, 2327, 3098], 'changes.': [138, 398, 2328], 'roGFP1': [139, 168, 187, 399, 428, 447, 2334, 4102], 'has': [140, 400, 605, 877, 1305, 1384, 1403, 1584], 'targeted': [142, 402, 1873], 'mitochondria': [145, 197, 405, 457, 533, 2399], 'HeLa': [147, 195, 246, 407, 455, 506, 2401], 'cells.': [148, 408], 'Fluorometric': [149, 409], 'measurements': [150, 410, 4020], 'these': [152, 412, 1413, 2072, 2290, 3644], 'using': [154, 162, 414, 422, 2363, 3499, 3575, 3983, 4338, 4357], 'microscope': [157, 417, 2366], 'suspension': [161, 421], 'fluorometer': [164, 424, 2374, 4086], 'probe': [169, 188, 429, 448, 2342], 'is': [170, 198, 240, 248, 430, 458, 500, 508, 537, 587, 1291, 1355, 1429, 1528, 1632, 2058, 2089, 2165, 2403, 2761, 2887, 3326, 3437, 3661, 3671, 3679, 3795, 3829, 3838, 3845, 3872, 4115, 4131], 'dynamic': [172, 432, 1537, 2757, 2883, 4111], 'equilibrium': [173, 433, 2710, 3285, 3314, 3504, 3516, 3559, 3624, 3631, 3950], 'mitochondrial': [176, 209, 258, 436, 469, 518, 1050, 3014], 'status': [178, 438, 617, 1304, 1316, 1521, 1539, 1901], 'responds': [180, 440], 'membrane-permeable': [182, 442, 2032, 2352], 'reductants': [183, 443], 'oxidants.': [185, 445], 'The': [186, 446, 1848, 1998, 2039, 2230, 2505, 2635, 2664, 2882, 3029, 3125, 3164, 3380, 3484, 3503, 3753, 3929, 4074, 4110], 'reports': [189, 449, 2344], 'matrix': [192, 452], 'space': [193, 453], 'reducing,': [200, 460], 'midpoint': [203, 463, 2279, 2783, 2798, 2911, 3419, 3934], 'near': [205, 465, 2404], '-360': [206, 466, 2405], 'mV': [207, 467], '(assuming': [208, 468], 'pH': [210, 470, 1865, 2383, 2753, 2878, 3202, 3292, 3759, 3808, 3930, 3982], '∼8.0': [211, 471], '37': [213, 473], '°C).': [214, 474], 'In': [215, 475, 622, 734, 812, 3090, 3643, 3949], 'other': [216, 476, 544, 568, 947, 1018, 2174], 'work': [217, 477, 2643], '(C.': [218, 478], 'T.': [219, 221, 479, 481, 1454, 1711, 3360, 3362], 'Dooley,': [220, 480, 3361], 'M.': [222, 482, 1620, 1665, 1692, 1826, 1967, 2113, 2551, 2613, 2617, 3020, 3363, 3604, 4044], 'Dore,': [223, 483, 3364], 'G.': [224, 484, 1734, 2136, 3023, 3365, 4022], 'Hanson,': [225, 485, 3366], 'W.': [226, 486, 3367], 'C.': [227, 487, 1368, 2599, 3368], 'Jackson,': [228, 488, 3369], 'S.': [229, 489, 1700, 1723, 1760, 1783, 1794, 1830, 2117, 2625, 3058, 3370], 'J.': [230, 490, 838, 994, 1089, 1125, 1128, 1155, 1169, 1671, 1686, 1803, 1945, 2141, 2204, 3371, 3608, 3817, 3907, 4027], 'Remington,': [231, 491, 3372], 'R.': [233, 493, 1452, 1775, 2593, 3019, 3374], 'Y.': [234, 494, 979, 1062, 3375], 'Tsien,': [235, 495, 3376], 'submitted': [236, 496, 3377], 'publication),': [238, 498], 'it': [239, 499, 1402, 1428, 1527], 'shown': [241, 501, 2023], 'cytosol': [244, 504, 1485, 1507], 'also': [249, 509, 1014, 2041, 2717, 2840, 3032, 4006], 'unusually': [250, 510], 'reducing': [251, 511, 528, 1511, 2063, 2355], 'but': [252, 512, 874, 1646, 2269, 4005], 'somewhat': [253, 513], 'less': [254, 514, 3328], 'so': [255, 515, 2518], 'than': [256, 516, 1512, 3329], 'matrix.': [259, 519], 'Most': [520, 1324], 'reactions': [522, 834], 'take': [525], 'place': [526], 'compartments': [529], 'such': [530, 564, 577, 1021, 1145, 1259, 1425], 'as': [531, 578, 871, 1022, 1146, 1216, 1218, 1636, 1653, 2524, 3356, 3945, 4117, 4133, 4349], 'cytoplasm.': [535, 2067], 'because': [539], 'critical': [540], 'thiol': [541, 1363], 'groups': [542, 1911], 'key': [545, 1849], 'catalytic': [546], 'elements': [547], 'enzyme': [549, 1031, 1478], 'active': [550], 'sites': [551, 2261, 2531, 2639], 'must': [552], 'be': [553, 584, 940, 1015, 1110, 1204, 1329, 1335, 1509, 1541, 1872, 1887, 1903, 2102, 2447, 2511, 3497, 3562, 3593, 3717, 3751, 3768, 3937, 3972, 4015], 'protected': [554], 'against': [555, 944, 2779, 2794, 2907, 3342, 3452, 3728, 3898, 4174], 'oxidation.': [556, 4012], 'Protein': [557, 2602, 2934], 'bonds': [559, 628, 826], 'rarely': [561], 'found': [562, 1386, 1405, 3425, 4317], 'environments.': [565], 'On': [566], 'hand,': [569], 'normal': [570], 'function': [572, 594], 'requires': [573], 'some': [575], 'compartments,': [576, 1423], 'endoplasmic': [580, 631], 'reticulum': [581, 632], 'eukaryotes,': [583], 'oxidizing.': [585], 'essential': [588], 'proper': [591, 3046], 'folding': [592, 3047], 'proteins': [596, 828, 1215], 'destined': [597], 'export,': [599], 'which': [600, 720, 1354, 1474, 1583, 2050, 2236, 3039, 3389, 4058], 'frequently': [601], 'contain': [602, 2718, 2841], 'bridges.Much': [604], 'recently': [606], 'learned': [608], 'regarding': [609], 'mechanisms': [611, 1008, 1257], 'responsible': [612, 1009, 2060], 'homeostasis': [614, 1012], 'certain': [619], 'subcellular': [620], 'compartments.': [621], 'yeast,': [623], 'formation': [625, 743, 821, 2306, 2587, 3050], 'utilizes': [633], 'conserved': [634], 'Ero1p': [635], '(2Frand': [636, 3851], 'A.R.': [637, 749, 792, 897, 955, 3775, 3852], 'Kaiser': [638, 686, 705, 750, 795, 900, 919, 956, 3776, 3853], 'C.A.': [639, 687, 706, 751, 796, 901, 920, 957, 3777, 3854], 'Mol.': [640, 661, 752, 775, 958, 1189, 2205, 3778, 3855], 'Cell.': [641, 662, 753, 776, 839, 959, 3779, 3856], '1998;': [642, 663, 800, 905, 960, 1603, 1672, 1702, 1741, 3857], '1:': [643, 664, 925, 961, 3858], '161-170Abstract': [644, 962, 3859], 'Full': [645, 647, 666, 668, 757, 759, 780, 782, 803, 805, 843, 845, 908, 910, 963, 965, 1675, 1677, 1809, 1811, 2147, 2186, 2188, 3783, 3785, 3860, 3862, 4033, 4051], 'Text': [646, 648, 667, 669, 758, 760, 781, 783, 804, 806, 844, 846, 909, 911, 964, 966, 1676, 1678, 1810, 1812, 2148, 2187, 2189, 3784, 3786, 3861, 3863, 4034, 4052], 'PDF': [649, 670, 761, 784, 807, 847, 912, 967, 1679, 1813, 2149, 2190, 3787, 3864, 4035, 4053], 'PubMed': [650, 671, 694, 715, 762, 785, 848, 927, 968, 985, 1069, 1081, 1095, 1134, 1180, 1195, 1377, 1464, 1606, 1626, 1680, 1705, 1728, 1744, 1765, 1788, 1814, 1843, 1949, 1982, 2130, 2150, 2191, 2210, 2566, 2607, 2630, 3064, 3612, 3788, 3865, 4036, 4333], 'Scopus': [651, 672, 695, 716, 763, 786, 808, 849, 913, 928, 969, 986, 1001, 1070, 1082, 1096, 1135, 1181, 1196, 1378, 1465, 1607, 1627, 1681, 1706, 1729, 1745, 1766, 1789, 1815, 1844, 1950, 1983, 2131, 2151, 2192, 2211, 2496, 2567, 2608, 2631, 3065, 3613, 3789, 3824, 3866, 3913, 4037, 4054, 4334], '(400)': [652, 970, 3867], 'Google': [653, 674, 697, 718, 765, 788, 810, 851, 915, 930, 971, 988, 1003, 1072, 1084, 1098, 1137, 1160, 1183, 1198, 1380, 1467, 1609, 1629, 1683, 1708, 1731, 1747, 1768, 1791, 1817, 1846, 1952, 1985, 2133, 2153, 2194, 2213, 2498, 2569, 2610, 2633, 3067, 3615, 3791, 3826, 3868, 3915, 4039, 4056, 4336], 'Scholar,': [654, 698, 916, 972, 989, 1073, 1085, 1161, 1610, 1618, 1684, 1709, 1732, 1748, 1769, 1792, 1818, 2134, 2195, 2611, 4040], '3Pollard': [655], 'M.G.': [656], 'Travers': [657], 'K.J.': [658], 'Weissman': [659, 773], 'J.S.': [660, 774], '171-182Abstract': [665], '(373)': [673], 'Scholar)': [675, 766, 1184, 1199, 1468, 1953, 1986, 2499, 2570, 3916], 'and/or': [676], 'Erv2p': [677], '(4Sevier': [678], 'C.S.': [679, 702], 'Cuozzo': [680, 793, 898], 'J.W.': [681, 794, 899, 918], 'Vala': [682], 'A.': [683, 704, 1163, 1690, 1701, 1713, 1717, 1724, 1761, 1771, 1784, 1939, 2626], 'Aslund': [684], 'F.': [685], 'Nat.': [688, 709, 921, 1063], 'Cell': [689, 798, 903, 922, 1190, 3145], 'Biol.': [690, 711, 799, 904, 923, 1065, 1804, 2142, 2182, 2206, 4028], '2001;': [691, 998, 1066, 1725, 1946], '3:': [692, 2605], '874-882Crossref': [693], '(153)': [696], '5Gross': [699], 'E.': [700, 3109], 'Sevier': [701], 'Vata': [703], 'Fass': [707], 'D.': [708, 992, 1175, 2199, 4289, 4329], 'Struct.': [710, 1064], '2002;': [712, 777, 982, 1192, 1623, 1840, 2127], '9:': [713, 2184], '61-67Crossref': [714], '(162)': [717], 'Scholar),': [719, 3792, 3869, 4057, 4303, 4337], 'FAD-containing': [722], 'enzymes': [723, 818], 'directly': [725, 1209, 1249, 1345, 4087], 'consume': [726], 'molecular': [727, 1590], 'oxygen': [728], 'create': [730], 'linkage.': [733], 'addition,': [735], 'exchange': [738, 833, 860], 'component': [739], 'protein-disulfide': [740], 'isomerase': [741], 'ensures': [742], 'correct': [746], 'disulfides': [747, 3404], '(6Frand': [748, 3774], '1999;': [754, 840, 924, 1092, 1131, 1177, 1461, 1762, 1785, 2183, 3780, 4330], '4:': [755, 3781], '469-477Abstract': [756, 3782], '(313)': [764, 3790], '(for': [767, 950, 1594], 'reviews,': [768, 951, 1595], 'see': [769, 952, 1596, 2651], 'Refs.': [770, 953, 1597], '7Tu': [771], 'B.P.': [772, 3054], '10:': [778, 801, 906], '983-994Abstract': [779], '(345)': [787], 'Scholar': [789], '8Frand': [791], 'Trends': [797, 902, 4045], '203-210Abstract': [802, 907], '(296)': [809, 914], 'Scholar).': [811, 931, 1099, 1138, 1381, 1630, 1847, 2154, 2634, 3068, 3616], 'prokaryotes,': [813], 'thioredoxin-like': [815], 'family': [816], 'catalyzes': [819, 4192], 'isomerization': [823], 'via': [829, 2938], 'specific': [831, 1236, 1875, 4003], '(9Debarbieux': [835], 'L.': [836, 1832, 2119], 'Beckwith': [837, 993], '99:': [841], '117-119Abstract': [842], '(77)': [850], 'Scholar).In': [852], 'all': [853, 1320, 2959], 'cells,': [854, 1526, 2981], 'small': [855, 937, 2175], 'molecules': [856], 'capable': [857], 'thiol-disulfide': [859], 'like': [861], 'GSH': [862, 887], 'abundant': [864, 1361], 'were': [866, 1498, 2012, 2022, 2358, 2415, 2538, 2667, 2680, 2936, 2962, 2993, 3127, 3183, 3209, 3259, 3270, 3297, 3335, 3384, 3424, 3533, 3637, 3887, 4082, 4226, 4258, 4280, 4306, 4316], 'previously': [867], 'thought': [868], 'act': [870, 1652], 'buffers,': [873], 'this': [875, 1516, 2642, 2715, 2838], 'hypothesis': [876], 'disproved.': [879], 'For': [880, 1040, 1337, 2435, 2977, 3619, 3893, 4219], 'example,': [881, 1041, 1338], 'export': [885], 'pathway,': [886, 1396], 'actually': [888], 'competes': [889], 'thiols': [892, 938], 'oxidizing': [894, 1996, 2353], 'equivalents': [895], '(8Frand': [896], '10Cuozzo': [917], '130-135Crossref': [926], '(254)': [929], 'Thus,': [932], 'primary': [934], 'role': [935], 'may': [939, 1013, 1518, 2322, 2428], 'one': [941, 1244], 'defense': [943, 1026], 'oxidants': [945], 'deleterious': [948, 2512], 'compounds': [949], '2Frand': [954], '11Dickinson': [973], 'D.A.': [974, 1737, 1752], 'Forman': [975], 'H.J.': [976], 'Ann.': [977], 'N.': [978], 'Acad.': [980, 1697, 1720, 1757, 1780, 2622], 'Sci.': [981, 1698, 1721, 1758, 1781, 2623, 4047], '973:': [983], '488-504Crossref': [984], '(378)': [987], '12Ritz': [991], 'Annu.': [995, 1600], 'Rev.': [996, 1601, 1622], 'Microbiol.': [997], '2001:': [999], '21-48Crossref': [1000], '(275)': [1002], 'Scholar).Where': [1004], 'required,': [1005], 'general': [1007, 1275, 1297], 'co-opted': [1016], 'diverse': [1019], 'apoptosis,': [1023], 'disease,': [1024], 'oxidative': [1025, 1042, 1260], 'mechanisms,': [1027], 'allosteric': [1028], 'control': [1029], 'activity,': [1032], 'variety': [1035], 'signal': [1037], 'transduction': [1038, 1256], 'pathways.': [1039], 'events': [1043], 'implicated': [1046], 'activating': [1048], 'permeability': [1051], 'transition,': [1052], 'leading': [1053, 1118], 'cytochrome': [1055], 'c': [1056], 'release': [1057], 'death': [1060], '(13Shi': [1061], '8:': [1067], '394-401Crossref': [1068], '(294)': [1071], '14Chernyak': [1074], 'B.V.': [1075], 'Biosci.': [1076], 'Rep.': [1077], '1997;': [1078], '17:': [1079], '293-302Crossref': [1080], '(58)': [1083], '15Hall': [1086], 'A.G.': [1087], 'Eur.': [1088], 'Clin.': [1090], 'Invest.': [1091], '29:': [1093], '238-245Crossref': [1094], '(301)': [1097], 'Changes': [1100], 'cellular': [1103, 1221, 1278, 1400], 'oxidation': [1104, 1234, 1922, 3507], 'suggested': [1108], 'final': [1112], 'step': [1113], 'apoptotic': [1116, 1122], 'process': [1117], 'degradation': [1120], 'bodies': [1123], '(16Cai': [1124], 'Jones': [1126], 'D.P.': [1127], 'Bioenerg.': [1129], 'Biomemb.': [1130], '31:': [1132], '327-334Crossref': [1133], '(100)': [1136], 'Soluble,': [1139], 'short': [1140], 'lived,': [1141], 'reactive': [1143], 'species': [1144, 3394], 'peroxides': [1147], '(17Rhee': [1148], 'S.G.': [1149, 1834, 2121], 'Bae': [1150], 'Y.S.': [1151], 'Lee': [1152], 'S.R.': [1153], 'Kwon': [1154], "Science's": [1156], 'STKE.': [1157], '2000;': [1158, 1615, 1806, 2207], 'http://stke.sciencemag.org/cgi/content/full/sigtrans;2000/53/pe1PubMed': [1159], '18Claiborne': [1162], 'Yeh': [1164], 'J.I.': [1165], 'Mallet': [1166], 'T.C.': [1167], 'Luba': [1168], 'Crane': [1170], 'J.E.': [1171, 1739], 'Charrier': [1172], 'B.': [1173, 1188], 'Paronage': [1174], 'Biochemistry.': [1176, 1839, 2126], '38:': [1178], '15407-15416Crossref': [1179], '(457)': [1182], 'NO': [1186], '(19Demple': [1187], 'Biochem.': [1191, 1602, 3607, 4046], '234:': [1193], '11-18Crossref': [1194], '(45)': [1197], 'now': [1201, 1263], 'recognized': [1202, 1264], 'second': [1205], 'messengers': [1206], 'can': [1208, 1228, 1334, 1540, 1871, 1902, 2446, 3592, 3716, 3936, 3970, 4014], 'modulate': [1210], 'activity': [1212, 1252], 'target': [1214, 1241], 'well': [1217, 3480, 4217], 'indirectly': [1219], 'couple': [1220, 3803], 'responses': [1222], 'external': [1224], 'events.': [1225], 'These': [1226], 'nitrosylation': [1232], 'cysteine': [1237, 2256, 2426, 2687], 'several': [1246, 1309, 1339, 2081, 2224], 'possible': [1247], 'states,': [1248], 'modulating': [1250], 'target.': [1254], 'Signal': [1255], 'involving': [1258, 3920], 'modifications': [1261, 2441], 'rival': [1266], 'phosphorylation': [1268], 'significance.Given': [1270], 'importance': [1272], 'interest': [1276], 'processes,': [1280], 'noninvasive': [1282], 'method': [1283, 1517], 'reporting': [1285], 'within': [1288, 1422, 1437, 1523, 1877, 3288], 'living': [1289, 1525], 'desirable.': [1293], 'Until': [1294], 'now,': [1295], 'no': [1296, 3391], 'approach': [1298, 1427], 'nondestructive': [1300], 'readout': [1301], 'available.': [1307], 'Whereas': [1308, 1412, 1515], 'vivo': [1314, 1593, 2390, 3052], 'proposed,': [1319], 'serious': [1322], 'limitations.': [1323, 2082], 'techniques': [1325], 'harvested': [1330], 'before': [1331, 2682], 'their': [1332, 2678], 'contents': [1333], 'analyzed.': [1336], 'attempts': [1340], 'made': [1343, 3888], 'measure': [1346, 4135], 'ratio': [1348, 1383, 3683, 3731, 4119], 'glutathione,': [1353], 'few': [1357], 'exceptions': [1358], 'most': [1360, 1633, 2528, 3268], 'nonprotein': [1362], 'eukaryotic': [1365], '(20Hwang': [1367], 'Sinskey': [1369], 'A.J.': [1370], 'Lodish': [1371], 'H.F.': [1372], 'Science.': [1373, 1978, 2562], '1992;': [1374, 2493], '257:': [1375], '1496-1502Crossref': [1376], '(1574)': [1379], 'range': [1388, 1407, 1863, 2283, 2758, 2884, 3567, 4112], '1:1': [1390], '3:1': [1392], 'secretory': [1395], 'total': [1399, 3245], 'material': [1401], '100:1': [1409], '300:1.': [1411], 'results': [1414, 2070, 2391, 3334], 'point': [1415, 3692], 'dramatic': [1417], 'differences': [1418], 'an': [1426, 1470, 1995, 2308, 2314, 2372, 3121, 3339], 'course': [1431], 'impossible': [1432], 'monitor': [1434], 'same': [1439], 'over': [1441, 1893, 3564], 'period': [1443, 1895], 'time.': [1445], 'Keese': [1446], 'et': [1447, 1934], 'al.': [1448, 1935], '(21Keese': [1449], 'M.A.': [1450], 'Saffrich': [1451], 'Dandekar': [1453], 'Becker': [1455], 'K.': [1456, 1971, 2201, 2555, 3024], 'Schirmer': [1457], 'R.H.': [1458, 3056], 'FEBS': [1459], 'Lett.': [1460], '447:': [1462], '135-138Crossref': [1463], '(16)': [1466], 'proposed': [1469], 'alternative': [1471, 3340], 'procedure': [1472], 'glutathione': [1479], 'reductase': [1480, 2056], 'microinjected': [1482], 'into': [1483, 1912, 2965, 2995, 3085, 3198, 4228], 'human': [1487], 'fibroblasts.': [1488], 'detection': [1490], 'color': [1492], 'crystals,': [1496, 4204], 'they': [1497], 'able': [1499], 'determine': [1501], 'more': [1510], '-0.270': [1513], 'V.': [1514, 2287], 'allow': [1519], 'determination': [1522, 3621], 'cumbersome.Indicators': [1529], 'give': [1531], 'visual': [1532, 2541], 'constructed': [1542, 1650, 1904, 2695], '(GFP),': [1548], '1The': [1549, 1566], 'abbreviations': [1550, 1567], 'used': [1551, 1568, 1635, 2523, 4016], 'are:': [1552, 1569], 'GFP,': [1553, 1570], 'protein;': [1556, 1573], 'roGFP,': [1557, 1574, 3677], 'redox-sensitive': [1558, 1575, 2292], 'GFP;': [1559, 1576], 'DTT,': [1560, 1577], 'dithiothreitol;': [1561, 1578], 'YFP,': [1562, 1579], 'yellow': [1563, 1580, 1962], 'protein.': [1565, 1582, 2434], 'become': [1585], 'popular': [1587], 'tool': [1588], 'labeling': [1591], '22Tsien': [1598], 'R.Y.': [1599, 1694, 1754, 1777, 1975, 2140, 2559, 4026, 4042], '67:': [1604], '509-544Crossref': [1605], '(4882)': [1608], '23Remington': [1611], 'S.J.': [1612, 1800, 1838, 1977, 2125, 2180, 2203, 2561], 'Methods': [1613], 'Enzymol.': [1614], '305:': [1616], '195-211Google': [1617], '24Zimmer': [1619], 'Chem.': [1621, 1805, 2143, 3819, 3909, 4029], '102:': [1624, 3822], '759-781Crossref': [1625], '(941)': [1628], 'GFP': [1631, 1852, 1870, 1906, 2267, 2272, 2412, 2475, 2549, 2955, 2972, 3229, 4340], 'often': [1634], 'passive': [1638], 'indicator': [1639, 1898, 2246], 'gene': [1641], 'expression': [1642, 2978, 3118], 'localization,': [1645], 'variants': [1647, 2666], 'indicators': [1654, 2040, 2226, 2231, 2276, 2414], 'intracellular': [1656], 'concentrations': [1657, 3540, 3632, 3654], 'H+,': [1659], 'Ca2+,': [1660], 'halide': [1662], 'ions': [1663], '(25Kneen': [1664], 'Farinas': [1666], 'J.F.': [1667], 'Verkman': [1668, 1801], 'A.S.': [1669, 1802], 'Biophys.': [1670], '74:': [1673], '1591-1599Abstract': [1674], '(566)': [1682], '26Llopis': [1685], 'McCaffery': [1687], 'J.M.': [1688], 'Miyawaki': [1689, 1716], 'Farquhar': [1691], 'Tsien': [1693, 1753, 1776, 1974, 2139, 2558, 4025], 'Proc.': [1695, 1718, 1755, 1778, 2620], 'Natl.': [1696, 1719, 1756, 1779, 2621], 'U.': [1699, 1722, 1759, 1782, 2624], '95:': [1703], '6803-6808Crossref': [1704], '(915)': [1707], '27Nagai': [1710], 'Sawano': [1712], 'Park': [1714, 1823, 2110], 'E.S.': [1715, 1824, 2111], '98:': [1726], '3197-3202Crossref': [1727], '(798)': [1730], '28Miesenbock': [1733], 'De': [1735, 3602], 'Angelis': [1736], 'Rothman': [1738], 'Nature.': [1740], '394:': [1742], '192-195Crossref': [1743], '(1928)': [1746], '29Baird': [1749], 'G.S.': [1750], 'Zacharias': [1751], '96:': [1763, 1786], '11241-11246Crossref': [1764], '(725)': [1767, 1790], '30Miyawaki': [1770], 'Griesbeck': [1772], 'O.': [1773], 'Heim': [1774], '2135-2140Crossref': [1787], '31Jayaraman': [1793], 'Haggie': [1795], 'P.': [1796, 2138, 2601, 4024], 'Wachter': [1797], 'R.M.': [1798, 2178, 2197], 'Remington': [1799, 1837, 1976, 2124, 2179, 2202, 2560], '275:': [1807, 3610], '6047-6050Abstract': [1808], '(255)': [1816, 1951], '32Hanson': [1819], 'G.T.': [1820, 2107], 'McAnaney': [1821, 2108], 'T.B.': [1822, 2109], 'Rendell': [1825, 2112], 'Yarbrough': [1827, 2114, 2198], 'D.K.': [1828, 2115, 4323], 'Chu': [1829, 2116], 'Xi': [1831, 2118], 'Boxer': [1833, 2120], 'Montrose': [1835, 2122], 'M.H.': [1836, 2123], '41:': [1841, 2128], '15477-15488Crossref': [1842, 2129], '(217)': [1845, 2132], 'properties': [1850, 2679, 2711, 3078, 3101], 'efficient': [1854], 'fluorescence,': [1855], 'protease': [1856], 'resistance,': [1857], 'stability': [1859], 'throughout': [1860], 'wide': [1862], 'solvent': [1867], 'conditions.': [1868], 'Moreover,': [1869], 'organelles': [1876], 'host': [1879], 'cell,': [1880], 'permitting': [1881], 'conditions': [1882], 'areas': [1885], 'monitored': [1888], 'time.An': [1897], 'by': [1907, 2026, 2091, 2167, 2234, 2500, 2540, 2777, 2792, 2905, 3113, 3170, 3185, 3337, 3426, 3511, 3521, 3597, 4169], 'introduction': [1908, 2533], 'redox-reactive': [1910], 'protein,': [1914, 2517, 3710], 'locations': [1916, 1989, 2008, 2436, 2578], 'where': [1917, 3793], 'change': [1919, 2766, 2892], 'introduced': [1926, 1954, 2010, 2937, 2964, 2994, 3084, 3448], 'group(s)': [1927], 'would': [1928, 2101], 'modify': [1929], 'properties.': [1932, 2459], 'Ostergaard': [1933], '(33Ostergaard': [1936], 'H.': [1937], 'Henriksen': [1938], 'Hansen': [1940], 'F.G.': [1941, 2488], 'Winther': [1942], 'J.R.': [1943], 'EMBO': [1944], '20:': [1947], '5853-5862Crossref': [1948], 'pairs': [1955, 2423, 2537], 'onto': [1958], 'surface': [1960, 2260, 2577], '(YFP)': [1965], '(34Ormo': [1966, 2550], 'Cubitt': [1968, 2552], 'A.B.': [1969, 2553], 'Kallio': [1970, 2200, 2554], 'Gross': [1972, 2556], 'L.A.': [1973, 2557], '1996;': [1979, 2563, 3061], '273:': [1980, 2564], '1392-1395Crossref': [1981, 2565], '(1902)': [1984, 2568], 'bridges': [1993], 'environment.': [1997], 'indicators,': [1999], 'designated': [2000], 'rxYFPNM': [2001, 2076], '(where': [2002], 'N': [2003], 'M': [2005], 'indicate': [2006], 'cysteines)': [2011], 'successfully': [2013], 'expressed': [2014, 3107], 'cytoplasm': [2017], 'Escherichia': [2019], 'coli': [2020, 3110], 'respond,': [2025], 'intensity,': [2030], 'agents': [2034], '4,4′-dithiodipyridine': [2035], 'dithiothreitol': [2037, 3460], '(DTT).': [2038], 'responded': [2042], 'mutational': [2044], 'disruption': [2045], 'trxB': [2048], 'gene,': [2049], 'codes': [2051], 'flavoenzyme': [2054], 'thioredoxin': [2055], 'partially': [2059], 'Although': [2068], 'efforts': [2073], 'promising,': [2075], 'probes': [2078, 2096, 2291, 3991], 'suffer': [2079], 'Because': [2083, 3312], 'only': [2084, 3587], 'intensity': [2086, 3682], 'affected': [2090], 'bond': [2093], 'formation,': [2094, 2464], 'not': [2098, 3074, 3103, 3400], 'difficult': [2103], 'calibrate': [2105], '(32Hanson': [2106], '35Grynkiewicz': [2135], 'Martin': [2137, 4023], '1985;': [2144, 4030], '260:': [2145, 4031], '3440-3450Abstract': [2146, 4032], '(80)': [2152, 4038], 'A': [2155], 'further': [2156], 'complication': [2157], 'arises': [2158], 'fact': [2161, 3429], 'YFP': [2163], 'quenched': [2166], 'low': [2169, 4220], 'concentration': [2170, 3674], 'halides': [2172], 'anions': [2176], '(36Wachter': [2177], 'Curr.': [2181], 'R628-R629Abstract': [2185], '(136)': [2193], '37Wachter': [2196], '301:': [2208], '157-171Crossref': [2209], '(133)': [2212], 'Scholar).Here,': [2214], 'we': [2215, 2338, 2465], 'describe': [2216], 'independent': [2218], 'development': [2219], 'preliminary': [2221], 'application': [2222, 2350], 'GFP-based': [2225], 'status.': [2229], 'excitation,': [2235], 'reduces': [2237], 'eliminates': [2239], 'measurement': [2240], 'errors': [2241], 'resulting': [2242, 3030], 'concentration,': [2247, 4065], 'photobleaching,': [2248, 4069], 'variable': [2250, 4071], 'thickness.': [2252], 'Directed': [2253], 'selected': [2259, 2539], 'vicinity': [2264], 'chromophore': [2268, 2444, 3049, 3577], 'upon': [2270, 3442], 'different': [2271], 'backgrounds': [2273], 'resulted': [2274], 'apparent': [2278], 'potentials': [2280, 3919], '-0.27': [2284], '-0.29': [2286], 'We': [2288], 'term': [2289], '(roGFPs).Analysis': [2294], 'crystal': [2300, 4264], 'structures': [2301], 'verified': [2304, 2958], 'intramolecular': [2309, 3409, 3588], 'bridge': [2311], 'revealed': [2313, 3470, 3583], 'oxidation-dependent': [2315], 'strain': [2316, 2431, 3111], 'polypeptide': [2319], 'backbone': [2320], 'observed': [2326, 2764, 2890], 'Using': [2329], 'cultured': [2330], 'mammalian': [2331, 2980], 'expressing': [2333], 'mitochondria,': [2337, 2387], 'show': [2339], 'reversibly': [2343, 3440], 'agents.': [2356], 'Measurements': [2357], 'conducted': [2359], 'suspensions': [2370], 'ordinary': [2373], 'comparable': [2376], 'results.': [2377], 'After': [2378], 'correcting': [2379], 'estimated': [2382, 2776, 2791, 2904], '(∼8.0)': [2384], 'suggest': [2392], 'resting': [2398], 'mV.MATERIALS': [2406], 'AND': [2407], 'METHODSStructure-based': [2408], 'Design': [2409], 'Redox-sensitive': [2411], 'Variants—roGFP': [2413], 'designed': [2416], 'concept': [2419], 'cross-links': [2421], 'between': [2422], 'induce': [2429], 'close': [2437], 'chromophore,': [2440], 'environment': [2445], 'expected': [2448], 'emission': [2458], 'To': [2460, 3069, 3253], 'prevent': [2461], 'undesirable': [2462], 'cross-link': [2463], 'attempted': [2466], 'replace': [2468], 'present': [2472], 'wild-type': [2474, 2970], '48': [2478], '70': [2480], '(38Prasher': [2481], 'D.C.': [2482], 'Eckenrode': [2483], 'V.K.': [2484], 'Ward': [2485], 'W.W.': [2486], 'Prendergast': [2487], 'Cormier': [2489], 'M.J.': [2490], 'Gene': [2491, 2931, 3059], '(Amst.).': [2492, 3060], '11:': [2494, 4049], '229-233Crossref': [2495], '(1748)': [2497], 'serine': [2501], 'alanine,': [2503], 'respectively.': [2504, 4205], 'Cys70': [2508], 'proved': [2509], 'obtaining': [2514], 'soluble,': [2515], 'C48S': [2520, 2724, 2847], 'construct': [2521], 'was': [2522, 2694, 3083, 3106, 3147, 3152, 3168, 3286, 3917, 4145, 4355], 'background': [2526, 2673], 'cases.': [2529], 'Candidate': [2530], 'inspection': [2542], 'atomic': [2545], 'model': [2546], 'S65T': [2548, 2703, 4341], '(Protein': [2571, 4344], 'Data': [2572, 4278, 4345], 'Bank': [2573, 4346], 'code': [2574, 4347], '1EMA)': [2575, 4348], 'approximately': [2580], 'required': [2582], 'separation': [2583], 'orientation': [2585], 'linkage': [2591], '(39Sowdhamini': [2592], 'Srinivasan': [2594], 'N.B.S.': [2595], 'Santi': [2596], 'D.V.': [2597], 'Ramakrishnan': [2598], 'Balaram': [2600], 'Eng.': [2603], '1989;': [2604, 2627], '95-103Crossref': [2606], '(157)': [2609], '40Matsumura': [2612], 'Becktel': [2614], 'W.J.': [2615, 3904], 'Levitt': [2616], 'Matthews': [2618], 'B.W.': [2619], '86:': [2628], '6562-6566Crossref': [2629], '(256)': [2632], 'sets': [2637, 4279], 'reported': [2640], 'included': [2644], 'Ser147': [2645], 'Gln204': [2647], '(roGFP1': [2648, 2990], '-2;': [2650], 'Table': [2652], 'I)': [2653], 'Asn149': [2655], 'Ser202': [2657], '(roGFP3': [2658], '-4),': [2660], 'each': [2661, 4266], 'flanking': [2662], 'His148.': [2663], 'single-cysteine': [2665], 'created': [2668], 'either': [2670, 4248], 'pseudo-wild': [2671], 'type': [2672], '(C48S/Q80R)': [2674], '(C48S/Q80R/S65T),': [2676], 'investigated': [2681], 'construction': [2683], 'double': [2686, 2691], 'substitutions.': [2688], 'Finally,': [2689], 'variant': [2693], '(roGFP5': [2696], '-6)': [2698], 'without': [2701], 'mutation.Table': [2704], 'ISpectroscopic': [2705], 'ratios': [2707, 3703, 4077], 'roGFPsVariantaVariants': [2713], 'column': [2716, 2839, 3156], 'phenotypically': [2721, 2844], 'neutral': [2722, 2845], 'substitutions': [2723, 2846], 'Q80R.IDKeqbEquilibrium': [2726], 'constant': [2727, 2851, 3315, 3517, 3832, 3875], '(Keq)': [2728, 2852, 3518], 'values': [2729, 2750, 2853, 2875, 3421, 4125], 'given': [2731, 2855, 3520], 'reaction': [2734, 2858, 3318, 3382, 3486], 'roGFPred': [2735, 2859, 3319], '+': [2736, 2739, 2860, 2863, 3320, 3324, 3547], 'DTTox': [2737, 2861, 3321, 3512, 3544, 3548, 3636], 'roGFPox': [2738, 2862, 3323], 'DTTred': [2740, 2864, 3325, 3542, 3546, 3634, 3656], '(see': [2741, 2865, 3331, 3410], 'exception': [2742, 2866], 'below': [2743, 2867, 3411], 'lipoic': [2745, 2780, 2795, 2869, 2908, 3346, 3464], 'acid': [2746, 2781, 2796, 2870, 2909, 3347], 'Footnote': [2748, 2872], "f).E0'cE0'": [2749], '7': [2754, 2879, 3143, 3760, 3809], '30°C.δdThe': [2756], 'value': [2759, 2885], '(δ)': [2760, 2886], 'maximum': [2763, 2889, 4122, 4138], 'δ-fold': [2765, 2891], 'peak': [2769, 2895], 'ratio.VS147C/Q204CroGFP10.070−0.2886.1eMeasured': [2770], '475': [2772, 2807, 2817, 2827, 2899, 4096], 'nm.S147C/Q204CfValues': [2775], 'titration': [2778, 2793, 2906, 3341, 3738, 3897], '(assumed': [2782, 2797, 2910], 'potential,': [2784, 2799, 2912], '−290': [2785, 2800, 2913], 'mV),': [2786, 2801, 2914], 'average': [2787, 2802, 2915], 'experiments.roGFP10.83fValues': [2790], 'experiments.−0.2874.5eMeasured': [2805], 'nm.S147C/Q204C/S65TroGFP20.021−0.2725.8gMeasured': [2810], 'nm.N149C/S202CroGFP30.151−0.2994.3eMeasured': [2815], 'nm.N149C/S202C/S65TroGFP40.056−0.2862.6gMeasured': [2820], 'nm.S147C/Q204C/N149C/S202CroGFP50.134−0.2967.8eMeasured': [2825], 'nm.S147C/Q204C/N149C/S202C/S65TroGFP60.039−0.2805.4gMeasured': [2830], 'nm.a': [2835], 'Variants': [2836], 'Q80R.b': [2849], 'Equilibrium': [2850], 'f).c': [2873], "E0'": [2874, 3794, 3979], '30°C.d': [2881], 'ratio.e': [2896], 'Measured': [2897, 2919], 'nm.f': [2902], 'Values': [2903], 'experiments.g': [2918], 'nm.': [2924], 'Open': [2925], 'table': [2926], 'new': [2929], 'tab': [2930], 'Construction': [2932], 'Expression—Mutations': [2935], 'QuikChange™': [2940], 'kit': [2941], '(Stratagene,': [2942], 'La': [2943], 'Jolla,': [2944], 'CA),': [2945], 'following': [2946], "manufacturer's": [2948], 'protocol.': [2949], 'DNA': [2950], 'sequencing': [2951], 'entire': [2954], 'coding': [2956], 'sequence': [2957, 3005], 'mutations.': [2960], 'Mutations': [2961], 'initially': [2963], 'His-tagged': [2967], 'version': [2968], '(Q80R)': [2971], 'plasmid': [2975, 2997, 3031], 'pRSETB.': [2976], 'mutations': [2983, 4004], 'C48S/T65S/S147C/Q204C': [2984], '(which': [2985, 4191], 'contains': [2986], 'S65T)': [2987], 'C48S/S147C/Q204C': [2989], '-2)': [2992], 'pEGFP-N1': [2998], '(Clontech)': [2999], 'containing': [3000], 'addition': [3002], 'leader': [3004], 'E1α': [3008], 'subunit': [3009], 'pyruvate': [3011], 'dehydrogenase': [3012], 'targeting': [3015], '(a': [3016], 'gift': [3017], 'Brown': [3021], 'Brown,': [3025], 'University': [3026, 4298], 'Oxford).': [3028], 'contained': [3033, 4207], 'so-called': [3035], '“folding': [3036], 'mutation”': [3037], 'F64L,': [3038], 'seems': [3040], 'improve': [3042], 'efficiency': [3044], '(41Cormack': [3053], 'Valdivia': [3055], 'Falkow': [3057], '173:': [3062], '33-38Crossref': [3063], '(2489)': [3066], 'verify': [3070], 'F64L': [3072], 'did': [3073], 'significantly': [3075], 'alter': [3076], 'roGFPs,': [3080], 'mutation': [3082], 'original': [3087], 'pRSETB': [3088, 3117], 'plasmid.': [3089], 'side-by-side': [3091], 'experiments,': [3092], 'roGFP2/F64L': [3095], 'had': [3096], 'indistinguishable': [3097], '(data': [3102, 3399], 'shown).Mutant': [3104], 'JM109(DE3)': [3112], 'use': [3114], 'system': [3119, 3749], 'N-terminal': [3122, 3165], 'His6': [3123], 'tag.': [3124], 'resuspended': [3128], '50': [3130], 'mm': [3131, 3136, 3200, 3232, 3237, 3240, 3244, 3349, 4153, 4197, 4254], 'HEPES': [3132, 3233, 4154], '(pH': [3133, 3234, 4155, 4178, 4235], '7.9),': [3134], '300': [3135], 'NaCl,': [3137, 3238], '10%': [3138], 'glycerol,': [3139], 'sonicated': [3141], 'min.': [3144], 'lysate': [3146], 'centrifuged,': [3148], 'supernatant': [3151], 'applied': [3153], 'Ni2+-nitrilotriacetic': [3158], 'acid-agarose': [3159], 'resin': [3160], '(Qiagen,': [3161], 'Hilden,': [3162], 'Germany).': [3163], 'histidine': [3166], 'tag': [3167], 'removed': [3169], 'incubation': [3171], '1:50': [3173], '(w/w)': [3174], 'γ-chymotrypsin': [3175], '20': [3177, 3199, 4152], 'h': [3178, 3290, 3304], '22': [3180], '°C.': [3181, 3223], 'Samples': [3182, 3224], 'concentrated': [3184, 4146], 'filtration': [3186], '(Centricon': [3187], '10;': [3188], 'Amicon)': [3189], 'buffer-exchanged': [3191], 'PD-10': [3193], 'Sephadex': [3194], 'columns': [3195], '(Amersham': [3196], 'Biosciences)': [3197], 'HEPES,': [3201], '7.9.Spectroscopy': [3203], 'Redox': [3205], 'Titrations—Fluorescence': [3206], 'spectra': [3208, 3467], 'collected': [3210, 4259], 'Hitachi': [3212], 'F4500': [3213], 'PerkinElmer': [3215], 'Life': [3216], 'Sciences': [3217], 'LS55': [3218], 'spectrophotometers': [3220], '30': [3222, 3300, 3762, 3811, 3968], 'consisted': [3225], '1': [3227, 3239, 3243, 3289, 3330, 3523, 3528, 3550], 'μm': [3228, 4189, 4250], '75': [3231], '7.0),': [3235], '140': [3236], 'EDTA,': [3241], 'DTT': [3246, 3494, 4198], '(mixture': [3247], 'forms).': [3252], 'reduce': [3254, 4059], 'air': [3255], 'oxidation,': [3256], 'solutions': [3258, 3269, 4315], 'degassed': [3260], 'subsequently': [3262], 'flushed': [3263], 'nitrogen.': [3265], 'Where': [3266], 'possible,': [3267], 'prepared': [3271], 'allowed': [3273], 'equilibrate': [3275], 'glove': [3278], 'box': [3279], 'under': [3280], 'nitrogen': [3282], 'atmosphere.In': [3283], 'general,': [3284], 'obtained': [3287], '7.0.': [3293], 'However,': [3294], 'samples': [3296], 'incubated': [3298, 3534], '°C': [3301, 3763, 3812, 3969], '4': [3303, 4208, 4214], 'ensure': [3306], 'equilibration': [3307], 'GFP-DTT': [3310], 'system.': [3311], '↔': [3322], 'much': [3327], '“Results”),': [3332], 'checked': [3336], 'performing': [3338], '(10': [3348], 'above': [3352], 'buffer)': [3353], 'roGFP1,': [3355], 'described': [3357], 'elsewhere.': [3358], '2C.': [3359], 'publication.': [3379], 'equilibrated': [3381, 3451], 'mixtures': [3383], 'subjected': [3385], 'SDS-PAGE': [3387, 3580], 'analysis,': [3388], 'showed': [3390], 'evidence': [3392], 'corresponding': [3395, 3515], 'disulfide-linked': [3397], 'dimers': [3398], 'shown).': [3401], 'Therefore,': [3402], 'any': [3403, 3713, 3981], 'formed': [3407], 'crystallographic': [3413], 'evidence).Determination': [3414], 'Midpoint': [3416], 'Potentials—Apparent': [3417], 'exploiting': [3427], 'spectrum': [3434, 4091], 'strongly': [3438], 'dependent': [3441], 'cysteines.': [3449], 'When': [3450, 3531], 'buffers': [3454], 'consisting': [3455], '(DTTred,': [3461], 'DTTox)': [3462], 'acid,': [3465], 'amplitude': [3471], 'defined': [3481, 4116], 'isosbestic': [3482, 3691], 'point.': [3483], 'could': [3495, 3561, 3750, 3766], 'thus': [3496, 3971], 'analyzed': [3498], 'two-state': [3501], 'model.': [3502, 4352], 'roGFP': [3510, 3557, 3667, 3943, 3966, 3990], 'Reaction': [3522], 'Equation': [3525, 3598, 3742, 3984], '1.': [3526], 'roGFPred+DTTox↔roGFPox+DTTredReaction': [3527], 'Keq=[roGFPox][DTTred]/[roGFPred][DTTox](Eq.': [3529], '1)': [3530], 'presence': [3537], 'varying': [3539], '(total': [3545], '=': [3549, 3955, 3959], 'mm),': [3551], 'fractional': [3553, 3663], 'amount': [3554, 3664], '(R)': [3560], 'measured': [3563], 'whole': [3566], 'fluorescence.': [3578], 'Since': [3579], 'crystallography': [3582], 'R': [3591, 3660, 3727, 3828], 'related': [3594], 'Keq': [3596, 3745, 3958], '2': [3599, 3948], '(42Hawkins': [3600], 'H.C.': [3601], 'Nardi': [3603], 'Freedman': [3605], 'R.B.': [3606], '1991;': [3609, 3910], '341-348Crossref': [3611], '(84)': [3614], 'R=([DTTred]/[DTTox])/(Keq+[DTTred]/[DTTox])(Eq.': [3617], '2)': [3618], 'experimental': [3620], 'constants': [3625], 'roGFP-dithiothreitol': [3628], 'system,': [3629], 'calculated': [3638, 3769, 3973], 'according': [3639, 3740], 'Equations': [3641], '3-5.': [3642], 'expressions,': [3645], '[DTTred': [3646], 'o]': [3647, 3650], '[DTTox': [3649], 'initial': [3653, 3673], 'DTTox,': [3658], 'equilibrium,': [3669], '[roGFPo]': [3670], 'F': [3678, 3871], 'versus': [3689], '(425': [3693], 'nm),': [3694], 'Fox': [3696], 'Fred': [3698], '490/425': [3701], 'completely': [3706], 'although': [3712], 'wavelengths': [3715], 'chosen.': [3718], '[DTTred]=[DTTred0]-R[roGFP0](Eq.': [3719], '3)': [3720], '[DTTox]=[DTTox0]-R[roGFP0](Eq.': [3721], '4)': [3722], 'R=(F-Fox)/(Fred-Fox)(Eq.': [3723], '5)': [3724], 'plotting': [3726], '[DTTred]/[DTTox]': [3730], 'fitting': [3733], 'data': [3735, 4223, 4257, 4358], 'curve': [3739, 3885], '2,': [3743], 'roGFP/dithiothreitol': [3748], 'found.': [3752], '(': [3764, 3804], "E0(roGFP)')": [3765], 'then': [3767, 4363], 'Nernst': [3772, 3976], 'equation': [3773, 3977], "biochemist's": [3797], 'standard': [3798], 'DTTred/DTTox': [3802], "E0(DTT)'=-0.323": [3805], 'V,': [3806], '(43Sajewski': [3813], 'R.P.': [3814], 'Whitesides': [3815, 3905], 'G.M.': [3816, 3906], 'Am.': [3818], 'Soc.': [3820], '1980;': [3821], '2011-2015Crossref': [3823], '(381)': [3825], 'Scholar)),': [3827], 'gas': [3831], '(8.315': [3833], 'J': [3834], 'K-1': [3835], 'mol-1),': [3836], 'T': [3837], 'absolute': [3840], 'temperature': [3841, 4165, 4221], '(303.15': [3842], 'K),': [3843], 'n': [3844], 'number': [3847], 'transferred': [3849], 'electrons': [3850], 'Faraday': [3874], '(9.649': [3876], '×': [3877], '104': [3878], 'C': [3879], 'mol-1).': [3880], "E0(roGFP)'=E0(DTT)'=(RT/nF)×lnKeq(Eq.": [3881], '6)': [3882], 'Plots': [3883], 'fits': [3886], 'KaleidaGraph': [3890], '(Abelbeck': [3891], 'Software).': [3892], 'case': [3895], 'reduced/oxidized': [3899], 'lipoate,': [3900], "E0(Lip)'=-0.290": [3901], 'V': [3902], '(44Lees': [3903], 'Org.': [3908], '58:': [3911], '642-647Crossref': [3912], '(180)': [3914], 'assumed.Redox': [3918], 'liberation': [3922], 'H+': [3924], 'depend': [3925, 4000, 4007], 'intrinsically': [3926], 'pH.': [3928], 'dependence': [3931, 4062], 'seen': [3938], 'half-reaction': [3941], 'reduction': [3944], 'follows.': [3946], 'roGFPox+2H++2e-↔roGFPredReaction': [3947], 'midpoint,': [3953], '[roGFPox]': [3954], '[roGFPred]': [3956], '[H+]2.': [3960], 'Theoretically,': [3961], '7.': [3985], "E0'pH=E0'-60.1mV×(pH-7)(Eq.": [3986], '7)': [3987], 'Ratiometric': [3988], 'Measurements—The': [3989], 'whose': [3997], 'relative': [3998], 'amplitudes': [3999], 'make': [4018], '(35Grynkiewicz': [4021], '45Tsien': [4041], 'Poenie': [4043], '1986;': [4048], '450-455Abstract': [4050], '(355)': [4055], 'eliminate': [4061], 'sample': [4066], 'path': [4067], 'length,': [4068], 'source': [4072], 'intensity.': [4073], 'determined': [4083], 'typically': [4093], '(2.5-nm': [4098], 'slit': [4099], 'width)': [4100], 'roGFP2.': [4109], 'parameter': [4113], 'δ': [4114], 'minimum': [4124], 'ratios,': [4129], 'useful': [4132], 'attainable': [4139], 'signal/noise': [4140], 'ratio.Crystal': [4141], 'Structure': [4142], 'Determination—roGFP2/F64L': [4143], '(C48S/F64L/S65T/Q80R/S147C/Q204C)': [4144], '∼20': [4148], 'mg': [4149], 'ml-1': [4150], '7.9)': [4156], '0.2-μm-filtered.': [4158], 'Flat': [4159], 'plate-like': [4160], 'grew': [4162], 'room': [4164], '1-2': [4167], 'days': [4168], 'hanging': [4170], 'drop': [4171], 'vapor': [4172], 'diffusion': [4173], '0.1': [4175, 4232], 'm': [4176, 4181, 4233, 4238], 'Tris': [4177, 4234], '8.5),': [4179, 4236], '0.07-0.1': [4180, 4237], 'LiSO4,': [4182, 4239], '17-22%': [4183], 'polyethylene': [4184, 4241], 'glycol': [4185, 4242], '4000,': [4186, 4243], '0.5': [4188, 4249], 'CuCl2': [4190, 4251], 'formation)': [4194], '5': [4196, 4253], 'Drops': [4206], 'μl': [4209, 4215], 'solution': [4212, 4230], 'solution.': [4218], 'diffraction': [4222, 4256], 'collection,': [4224], 'exchanged': [4227], '20%': [4240, 4244], 'ethylene': [4245], 'glycol,': [4246], 'DTT.X-ray': [4255], 'frozen': [4263], 'beamline': [4270], '7-1': [4271], 'Stanford': [4274], 'Synchrotron': [4275], 'Radiation': [4276], 'Laboratory.': [4277], 'indexed': [4281], 'MOSFLM': [4285], '(46Leslie': [4286], 'A.G.W.': [4287], 'Moras': [4288], 'Podjarny': [4290], 'A.D.': [4291], 'Thierry': [4292], 'J.C.': [4293], 'Crystallographic': [4294], 'Computing.': [4295], '5.': [4296], 'Oxford': [4297], 'Press,': [4299], 'Oxford,': [4300], 'UK1996:': [4301], '50-61Google': [4302], 'intensities': [4305], 'scaled': [4307], 'SCALA': [4309], '(CCP4': [4310], 'program': [4311], 'suite).': [4312], 'Molecular': [4313], 'replacement': [4314], 'EPMR': [4319], '(47Kissinger': [4320], 'C.R.': [4321], 'Gehlhaar': [4322], 'Fogel': [4324], 'D.B.': [4325], 'Acta': [4326], 'Crystallogr.': [4327], 'Sec.': [4328], '55:': [4331], '484-491Crossref': [4332], '(689)': [4335], 'coordinate': [4342], 'file': [4343], 'search': [4351], 'Positional': [4353], 'refinement': [4354], 'begun': [4356], '3.5-Å': [4360], 'resolution': [4361], 'increased': [4364], 'stages': [4366], 'limi': [4369]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2069271483', 'counts_by_year': [{'year': 2024, 'cited_by_count': 32}, {'year': 2023, 'cited_by_count': 41}, {'year': 2022, 'cited_by_count': 49}, {'year': 2021, 'cited_by_count': 55}, {'year': 2020, 'cited_by_count': 47}, {'year': 2019, 'cited_by_count': 67}, {'year': 2018, 'cited_by_count': 49}, {'year': 2017, 'cited_by_count': 58}, {'year': 2016, 'cited_by_count': 69}, {'year': 2015, 'cited_by_count': 59}, {'year': 2014, 'cited_by_count': 69}, {'year': 2013, 'cited_by_count': 69}, {'year': 2012, 'cited_by_count': 62}], 'updated_date': '2024-12-11T06:59:40.687879', 'created_date': '2016-06-24'}