Title: Detection of factors that interact with the human β-interferon regulatory region in vivo by DNAase I footprinting
Abstract: We have used a DNAase I genomic footprinting procedure to detect interactions between cellular factors and the regulatory sequences of the human β-interferon gene. Prior to induction with poly(I)-poly(C), factors that bind to DNA are detected in one region located between −94 and −167 from the mRNA cap site, and in another region located between −68 and −38. After induction these factors dissociate and another factor binds to a region located between −77 and −64. Correlation of these footprints with the effects of deletions in the regulatory region of the β-interferon gene (accompanying paper) suggest that the factors that bind prior to induction are repressor molecules, while the component that binds after induction is a transcription factor. Dissociation of the repressor molecules from the DNA after induction may allow the transcription factor to bind to and activate the β-interferon promoter. Thus, the β-interferon gene may be controlled by a negative regulatory mechanism.
Publication Year: 1986
Publication Date: 1986-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 188
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