Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter

Name: Work Video:
Duration: 3 min 30 s
Xianqiang Li; Xiaoning Zhao; Yu Fang; Xin Jiang; Tommy Duong; Connie Fan; Chiao-Chain Huang; Steven R. Kain
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1321, 1467, 1585, 1592, 1636, 1647, 1683, 1701, 1835, 1876, 2025, 2048, 2171, 2221, 2378, 2410, 2436, 2463, 2481, 2501, 2581, 2601, 2619, 2654, 2673, 2730, 2734, 2759, 2831, 2927, 2943, 3081, 3111, 3124, 3210, 3239, 3250, 3301, 3430, 3590, 3611, 3654, 3691, 3717, 3721, 3818, 3849, 3922, 3971, 4040, 4046, 4064, 4161, 4175, 4178, 4184, 4203, 4224, 4334, 4380, 4396], 'gene': [11, 206, 229, 424, 542, 733, 1697, 2300], 'expression': [12, 230, 543, 1831, 1839, 1979, 2039, 2560], 'and': [13, 27, 93, 130, 155, 168, 231, 245, 311, 348, 373, 386, 544, 721, 728, 796, 1188, 1302, 1422, 1564, 1655, 1727, 1758, 1804, 1823, 1866, 1926, 1935, 1964, 1972, 2036, 2102, 2119, 2165, 2203, 2223, 2241, 2243, 2313, 2356, 2388, 2648, 2651, 2818, 2850, 2860, 2899, 2904, 2936, 3025, 3047, 3053, 3088, 3176, 3199, 3221, 3237, 3272, 3275, 3326, 3365, 3376, 3545, 3570, 3573, 3581, 3599, 3657, 3671, 3695, 3736, 3761, 3772, 3785, 3866, 3876, 3913, 3992, 3994, 4007, 4263], 'localization': [15, 233, 546, 3735], 'studies.': [16, 234, 1950], 'GFP': [17, 50, 79, 235, 268, 297, 623, 709, 795, 803, 846, 916, 948, 2254, 3931], 'stable': [20, 238, 918, 1318, 3427, 3441, 4196], 'protein;': [21, 239, 455, 460, 496, 501], 'this': [22, 33, 240, 251, 1500, 1608, 1861, 3646, 4200], 'property': [23, 241], 'allows': [24, 242, 4143], 'its': [25, 37, 196, 243, 255, 414, 438, 950, 1303, 1383, 1423, 1428, 1867, 2900, 3008, 3718, 3945, 4144, 4171, 4288, 4327], 'accumulation': [26, 244, 4177, 4328], 'easy': [28, 246], 'detection': [29, 247, 668, 1678, 2259, 2267, 3732], 'cells.': [31, 180, 249, 398, 1685, 1706, 2321, 2585, 2631, 3432], 'However,': [32, 250, 2950, 4014], 'stability': [34, 252, 946], 'also': [35, 253, 1457, 1721, 2536, 2913, 4343], 'limits': [36, 254, 949], 'application': [38, 256, 951, 4145], 'studies': [40, 55, 258, 273, 540, 4122, 4146, 4154], 'that': [41, 105, 259, 323, 801, 884, 974, 1386, 1510, 1599, 1749, 1794, 2588, 2686, 2709, 2782, 2983, 2998, 3129, 3194, 3383, 3684, 3696, 3828, 3841, 3955, 3967, 4017, 4193, 4244, 4285, 4316, 4364], 'require': [42, 260, 627, 1199, 3523], 'rapid': [43, 261, 1429, 3439, 3622, 4126, 4139, 4172, 4322], 'turnover.': [45, 263], 'We': [46, 119, 163, 264, 337, 381, 1642, 2981, 3127, 3953], 'created': [47, 265], 'destabilized': [49, 140, 198, 267, 358, 416, 466, 507, 1689, 3798, 4148, 4213], 'for': [51, 269, 665, 1202, 1390, 1459, 1833, 1948, 1977, 2099, 2108, 2147, 2182, 2343, 2382, 2416, 2439, 2460, 2644, 2846, 2909, 2915, 3034, 3117, 3269, 3277, 3340, 3404, 3449, 3487, 3566, 3757, 4011, 4121, 4281], 'use': [52, 270, 660, 1687, 1834, 3535, 3930], 'such': [54, 211, 272, 429, 987, 1043, 1205, 1597, 1716, 3148, 4034, 4150, 4403], 'by': [56, 148, 274, 366, 731, 972, 1117, 1190, 1571, 2013, 2034, 2043, 2162, 2228, 2233, 2663, 2702, 2962, 3108, 3145, 3280, 3461, 3745, 3780, 3800, 3852, 4096, 4354], 'fusing': [57, 275], 'amino': [58, 122, 276, 340, 718, 854, 879, 1573, 1645, 1870, 2493, 2526, 3076, 3099, 3159, 3172, 3977, 3985], 'acids': [59, 123, 277, 341, 682, 855, 880, 1646, 1871, 2494, 2527, 3077, 3173, 3978], '422–461': [60, 278], 'of': [61, 65, 74, 78, 91, 98, 109, 115, 128, 139, 195, 279, 283, 292, 296, 309, 316, 327, 333, 346, 357, 413, 437, 541, 672, 713, 793, 852, 877, 922, 947, 1120, 1284, 1293, 1313, 1347, 1351, 1462, 1507, 1581, 1604, 1615, 1623, 1628, 1634, 1651, 1664, 1672, 1679, 1688, 1709, 1731, 1768, 1783, 1788, 1872, 1960, 1980, 2046, 2080, 2144, 2174, 2278, 2359, 2405, 2490, 2547, 2566, 2592, 2609, 2613, 2657, 2669, 2712, 2744, 2761, 2768, 2785, 2799, 2812, 2816, 2871, 2894, 2946, 2969, 2978, 2986, 3002, 3020, 3023, 3074, 3091, 3139, 3154, 3167, 3183, 3202, 3215, 3219, 3224, 3234, 3297, 3322, 3362, 3420, 3425, 3452, 3500, 3603, 3614, 3624, 3660, 3674, 3687, 3698, 3714, 3724, 3727, 3733, 3739, 3787, 3805, 3811, 3821, 3831, 3860, 3863, 3870, 3881, 3958, 3965, 4025, 4049, 4061, 4100, 4103, 4123, 4128, 4141, 4205, 4215, 4227, 4237, 4245, 4257, 4261, 4270, 4274, 4287, 4305, 4310, 4317, 4324, 4349, 4367, 4386], 'the': [62, 71, 89, 106, 116, 125, 192, 216, 280, 289, 307, 324, 334, 343, 410, 434, 443, 530, 677, 797, 802, 893, 898, 903, 909, 981, 989, 1038, 1278, 1282, 1294, 1348, 1453, 1475, 1504, 1582, 1601, 1612, 1620, 1632, 1648, 1652, 1665, 1669, 1673, 1712, 1728, 1755, 1762, 1780, 1801, 1809, 1824, 1829, 1836, 1873, 1877, 1961, 1965, 1984, 2026, 2038, 2044, 2049, 2077, 2084, 2092, 2133, 2166, 2263, 2285, 2289, 2305, 2326, 2339, 2370, 2448, 2476, 2532, 2544, 2567, 2589, 2602, 2607, 2610, 2636, 2652, 2658, 2670, 2674, 2687, 2710, 2713, 2731, 2735, 2742, 2745, 2757, 2764, 2769, 2783, 2786, 2806, 2813, 2827, 2837, 2869, 2872, 2889, 2892, 2895, 2902, 2929, 2937, 2944, 2951, 2970, 2984, 2987, 2999, 3003, 3054, 3072, 3096, 3109, 3130, 3137, 3150, 3155, 3163, 3168, 3179, 3184, 3195, 3203, 3216, 3222, 3225, 3254, 3327, 3402, 3413, 3426, 3431, 3450, 3462, 3473, 3539, 3549, 3552, 3600, 3612, 3627, 3630, 3655, 3658, 3666, 3669, 3672, 3685, 3699, 3706, 3722, 3751, 3802, 3808, 3819, 3829, 3842, 3850, 3861, 3871, 3917, 3936, 3942, 3956, 4023, 4047, 4058, 4065, 4089, 4098, 4138, 4162, 4185, 4212, 4225, 4235, 4272, 4282, 4303, 4308, 4321, 4347, 4384, 4400], 'degradation': [63, 114, 281, 332, 998, 1430, 1461, 1516, 1587, 1613, 1626, 1949, 2500, 2618, 3080, 3107, 3131, 3803, 3970, 4018], 'domain': [64, 282, 1614, 1627, 2534, 3804], 'mouse': [66, 284, 462, 503, 1208, 1605, 1616], 'ornithine': [67, 285, 463, 504, 1209, 1311, 1391, 1465, 1606, 1617, 2498], 'decarboxylase': [68, 286, 1210, 1312, 1392, 1466, 1618, 2499], '(MODC)': [69, 287, 1211], 'to': [70, 151, 173, 203, 215, 288, 369, 391, 421, 433, 659, 689, 715, 723, 891, 1105, 1290, 1619, 1639, 1668, 1772, 1882, 2015, 2070, 2543, 2549, 2596, 2615, 2755, 2802, 2865, 2933, 3043, 3078, 3102, 3136, 3207, 3319, 3354, 3391, 3410, 3534, 3537, 3551, 3629, 3668, 3693, 3705, 3807, 3929, 3980, 4057, 4108, 4181, 4243, 4268, 4339, 4345], 'C-terminal': [72, 290, 1454, 2545], 'end': [73, 291, 1757, 1803, 2546], 'an': [75, 293, 888, 1805, 3516, 4118, 4393], 'enhanced': [76, 294, 457, 498], 'variant': [77, 295], '(EGFP).': [80, 298], 'fusion': [82, 117, 300, 335, 1473, 1653, 1666, 1825, 1878, 1958, 2245, 2553, 2568, 2671, 2688, 2714, 2747, 2765, 2787, 2897, 2938, 2953, 2971, 2989, 3004, 3012, 3056, 3120, 3814, 4202], 'protein,': [83, 301, 3164], 'unlike': [84, 302, 3434], 'EGFP,': [85, 303, 456, 497, 3235, 3398, 3514], 'was': [86, 111, 146, 189, 304, 329, 364, 407, 1720, 1745, 1770, 1790, 1826, 2198, 2205, 2260, 2301, 2555, 2570, 2578, 2623, 2661, 2681, 2875, 2931, 2948, 2958, 2973, 3014, 3133, 3143, 3248, 3334, 3371, 3506, 3594, 3609, 3634, 3642, 3676, 3702, 3766, 3778, 3844, 4342], 'unstable': [87, 305, 1471, 3817], 'presence': [90, 308, 3820, 3862], 'cycloheximide': [92, 310, 2138], 'had': [94, 312, 2700, 2795, 3652], 'fluorescence': [96, 107, 314, 325, 624, 671, 2078, 2127, 2183, 2564, 2574, 2590, 2655, 2664, 2667, 2698, 2732, 2760, 2773, 2800, 2814, 3000, 3112, 3278, 3504, 3937, 3946, 4376], 'half-life': [97, 315, 1304, 1633, 2711, 2743, 2784, 2893, 2985, 3830, 3843, 3851, 3924, 3947, 3957], '2': [99, 317, 1640, 2440, 2790, 2976, 3187, 3369, 3649, 3835, 3961], 'h.': [100, 318, 1641, 2418, 2441, 2720, 2791, 2852, 2994, 3680, 3710, 3763, 3836, 3846], 'Western': [101, 319, 2264, 2916, 3837, 3908], 'blot': [102, 320, 2917, 3018, 3838], 'analysis': [103, 321, 2811, 2918, 3019, 3233, 3494, 3839, 3999], 'indicated': [104, 322, 3827, 3840], 'decay': [108, 326, 3001], 'EGFP-MODC-(422–461)': [110, 129, 328, 347, 1865, 2593, 2905, 2952, 2988, 3220], 'correlated': [112, 330, 995, 4056], 'with': [113, 134, 208, 331, 352, 426, 734, 926, 996, 1474, 1514, 1560, 1659, 1704, 1711, 1735, 1747, 1779, 1792, 1932, 2020, 2105, 2137, 2155, 2214, 2262, 2288, 2304, 2330, 2365, 2375, 2390, 2400, 2423, 2444, 2452, 2474, 2598, 2640, 2696, 2826, 2842, 2858, 2919, 3007, 3060, 3267, 3350, 3478, 3497, 3558, 3564, 3575, 3583, 3596, 3605, 3755, 3768, 3824, 3878, 4022, 4043], 'protein.': [118, 336, 2766, 2939], 'mutated': [120, 338, 1643, 1881, 3084], 'key': [121, 339, 678, 1279, 1644], 'PEST': [126, 344, 990, 1420, 1511, 1583, 1649, 1874, 2523, 2533, 3075, 3140, 3204, 3217, 3974, 4026, 4090, 4101], 'sequence': [127, 345, 679, 685, 986, 993, 1584, 1650, 1753, 1760, 1799, 1807, 1875, 2281, 2524, 3205, 3218, 3325, 3975, 4067], 'identified': [131, 349, 1656], 'several': [132, 350, 1593, 1657], 'mutants': [133, 351, 1658, 1885, 3121, 4029], 'variable': [135, 353], 'half-lives.': [136, 354, 1661], 'suitability': [138, 356], 'EGFP': [141, 188, 199, 359, 406, 417, 467, 508, 1635, 1690, 1726, 1744, 1769, 2081, 2197, 2242, 2306, 2538, 2548, 2597, 2617, 2817, 2896, 2903, 2935, 2947, 3024, 3052, 3055, 3442, 3799, 3872, 3969, 4116, 4207, 4216, 4275, 4291, 4311], 'as': [142, 191, 212, 360, 409, 430, 988, 1044, 1503, 1598, 1692, 1717, 2073, 2678, 2960, 3093, 3095, 3149, 3256, 3294, 3312, 3388, 3401, 3446, 3932, 4035, 4151, 4220, 4239, 4370, 4404], 'transcription': [144, 362, 1674, 1694, 2012, 3314, 3393, 3543, 3725, 4241, 4372], 'tested': [147, 365, 2606], 'linking': [149, 367], 'it': [150, 368, 656, 661, 907, 2542, 2577, 3318, 3536, 4112, 4391], 'NFκB': [152, 161, 213, 370, 379, 431, 1676, 1681, 2279, 2354, 2429, 3323, 3343, 3456, 3546, 3579, 3604, 3608, 3625, 3651, 3663, 3688, 3701, 3715, 3734, 3764, 3783, 4253], 'binding': [153, 371, 1670, 2014, 2023, 2030, 2280, 3324], 'sequences': [154, 372, 1350, 1381, 1512, 1671, 4102], 'monitoring': [156, 374, 3341], 'tumor': [157, 375, 477, 518], 'necrosis': [158, 376, 478, 519], 'factor': [159, 377, 1675], 'α-mediated': [160, 378], 'activation.': [162, 380, 4254], 'obtained': [164, 177, 382, 395], 'time': [165, 383, 1703, 3290, 3347, 4258, 4335, 4382], 'course': [166, 384, 3348, 4259, 4336], 'induction': [167, 207, 385, 425, 957, 1682, 1698, 1710, 2358, 3349, 3544, 3593, 3659, 3673, 3726, 3738, 3777, 3786, 4260, 4309, 4337, 4348], 'dose': [169, 387, 4264], 'response': [170, 388, 3692, 4265], 'kinetics': [171, 389, 3351, 4256, 4273], 'similar': [172, 390, 2595, 3353, 4267, 4338], 'secreted': [174, 392, 473, 514], 'alkaline': [175, 393, 474, 515], 'phosphatase': [176, 394], 'transfected': [179, 397, 1936, 2066, 2153, 2212, 2318, 2364, 2371, 2626, 2659, 2825, 2838, 2906, 3240, 3336, 3460, 3744], 'This': [181, 399, 684, 1286, 1415, 1566, 2029, 2519, 2552, 3415, 3632, 3711, 4038], 'result': [182, 400, 3416, 3921], 'did': [183, 401, 2724, 3406], 'not': [184, 402, 626, 1198, 1388, 2725, 3134, 3407, 3522, 3643, 4020, 4359], 'occur': [185, 403], 'when': [186, 404, 1319, 3399, 4187], 'unmodified': [187, 405, 3397, 4206], 'reporter.': [193, 411, 3360, 4373], 'Because': [194, 412, 436, 3926, 4111, 4374], 'autofluorescence,': [197, 415], 'can': [200, 418, 1974, 2031, 2040, 2535, 3309, 3385, 4217, 4377], 'be': [201, 419, 1291, 1975, 2032, 2041, 3310, 3386, 3418, 3444, 4209, 4218, 4378], 'directly': [204, 422], 'correlate': [205, 423, 1513, 4021], 'biochemical': [209, 427, 1714], 'change,': [210, 428], 'translocation': [214, 432, 1719, 2355, 3547, 3601, 3623, 3686, 3713, 3765, 3784], 'nucleus.': [217, 435, 3553, 3631], 'easily': [439, 4114, 4293], 'detected': [440, 2206, 2248, 3059, 3644, 3790, 4379], 'fluorescence,': [442, 2901], '1The': [448, 489], 'abbreviations': [449, 490], 'are:': [451, 492], 'GFP,': [452, 493, 3906, 4149], 'MODC,': [461, 502, 2491], 'decarboxylase;': [464, 505], 'dEGFP,': [465, 506, 2360, 3082, 3092, 3435], '(EGFP-MODC-(422–461)': [468, 509], 'fusion);': [469, 510], 'Tc,': [470, 511, 2035], 'tetracycline;': [471, 512], 'SEAP,': [472, 513, 3355, 4405], 'phosphatase;': [475, 516], 'TNF,': [476, 517], 'factor;': [479, 520], 'CHX,': [480, 521, 2697], 'cycloheximide;': [481, 522], 'CHO,': [482, 523], 'Chinese': [483, 524], 'hamster': [484, 525], 'ovary;': [485, 526], 'PBS,': [486, 527, 2222, 2475], 'phosphate-buffered': [487, 528], 'saline.': [488], 'saline.from': [529], 'jellyfish': [531], 'Aequorea': [532], 'victoria': [533], '(1Chalfie': [547, 928], 'M.': [548, 587, 810, 929, 1004, 1021, 1049, 1068, 1165, 1401, 1438, 1532, 1543, 1842, 1988], 'Euskirchen': [549, 930], 'G.': [550, 931], 'Ward': [551, 860, 932], 'W.W.': [552, 861, 933], 'Prasher': [553, 634, 934], 'D.C.': [554, 635, 857, 935], 'Science.': [555, 821, 936, 1005, 1026, 1054, 1406, 1443, 1533], '1994;': [556, 647, 700, 937], '263:': [557, 938], '802-805Crossref': [558, 939], 'PubMed': [559, 581, 618, 650, 703, 748, 769, 786, 825, 841, 871, 940, 1009, 1030, 1058, 1084, 1099, 1132, 1146, 1159, 1179, 1229, 1246, 1271, 1343, 1374, 1410, 1447, 1496, 1537, 1855, 1908, 2001, 2514, 3896, 4083], 'Scopus': [560, 582, 593, 619, 651, 704, 749, 770, 787, 826, 842, 872, 941, 1010, 1031, 1059, 1085, 1100, 1133, 1160, 1247, 1272, 1411, 1448, 1538, 1856, 1909, 2002, 2515, 3897, 4084], '(5451)': [561, 942], 'Google': [562, 584, 595, 621, 653, 706, 751, 772, 789, 828, 844, 874, 943, 1012, 1033, 1061, 1087, 1102, 1135, 1147, 1162, 1180, 1230, 1249, 1274, 1344, 1375, 1413, 1450, 1497, 1540, 1550, 1858, 1911, 2004, 2517, 3899, 4086], 'Scholar,': [563, 585, 596, 752, 773, 1013, 1088, 1136, 1148, 1163, 1231, 1250, 1541], '2Marshall': [564], 'J.': [565, 754, 1170, 1220, 1241, 1334, 1365, 1487], 'Molloy': [566], 'R.': [567, 600, 633, 1002, 1530], 'Moss': [568, 831], 'G.W.': [569], 'Howe': [570], 'J.R.': [571], 'Hughes': [572], 'T.E.': [573], 'Neuron.': [574], '1995;': [575, 590, 612], '14:': [576, 839], '211-215Abstract': [577], 'Full': [578, 615, 764, 766, 1081, 1176, 1226, 1340, 1371, 1493], 'Text': [579, 616, 765, 767, 1082, 1177, 1227, 1341, 1372, 1494], 'PDF': [580, 617, 768, 1083, 1178, 1228, 1342, 1373, 1495], '(219)': [583], '3Chalfie': [586], 'Photochem.': [588], 'Photobiol.': [589], '60:': [591], '651-656Crossref': [592], '(250)': [594], '4Cubitt': [597], 'A.B.': [598, 812], 'Heim': [599], 'Adams': [601], 'S.R.': [602, 779, 1000, 1528], 'Boyd': [603], 'A.E.': [604], 'Gross': [605, 815], 'L.A.': [606, 816], 'Tsien': [607, 636, 817], 'R.Y.': [608, 637, 818], 'Trends': [609], 'Biochem.': [610, 1155, 1242], 'Sci.': [611, 641, 1848, 1994], '20:': [613], '448-455Abstract': [614], '(1193)': [620], 'Scholar).': [622, 707, 790, 845, 875, 944, 1034, 1103, 1181, 1275, 1345, 1414, 1451, 1498, 1551, 1859, 1912, 2518, 3900, 4087, 4169], 'does': [625, 3521, 4019], 'any': [628, 3524, 4387], 'substrate': [629], 'or': [630, 1063, 1577, 2082, 2156, 2215, 2307, 2338, 2348, 3467, 3491, 3496, 3526, 3729, 4231, 4389, 4408], 'cofactor': [631], '(5Heim': [632], 'Proc.': [638, 1845, 1991], 'Natl.': [639, 1846, 1992], 'Acad.': [640, 1847, 1993], 'U': [642], '.': [643, 645], 'S': [644, 1193], 'A.': [646, 1150, 1152, 1217, 1237, 1266, 1851, 1997], '91:': [648], '12501-12504Crossref': [649], '(1476)': [652], 'Scholar);': [654, 1376], 'hence': [655, 2037], 'possible': [658, 1700], 'many': [663], 'species': [664], 'live': [666], 'cell': [667, 2167, 2177, 2224, 3036, 3039, 4197], 'purposes.': [669], 'GFPs': [673], 'dependent': [675], 'on': [676, 1519, 2088, 2115], 'Ser-Tyr-Gly': [680], '(amino': [681], '65–67).': [683], 'undergoes': [686], 'spontaneous': [687], 'oxidation': [688], 'form': [690, 881, 892], 'cyclized': [692], 'chromophore': [693, 899, 3873], '(6Inouge': [694], 'S.': [695, 742, 1138, 1254, 1258, 1850, 1996, 3890], 'Tsuji': [696], 'F.I.': [697], 'FEBS': [698], 'Lett.': [699], '351:': [701], '211-214Crossref': [702], '(133)': [705], 'Enhanced': [708], '(EGFP)': [710], 'contains': [711, 1418, 2521], 'mutations': [712, 1914, 3238], 'Ser': [714, 4062], 'Thr': [716, 3089, 3200, 3993, 4008], 'at': [717, 725, 1382, 1600, 1754, 1761, 1800, 1808, 1820, 2096, 2140, 2200, 2385, 2525, 2955, 3158, 3171, 3288, 3373, 3935, 3941, 4001, 4131], 'acid': [719, 3100, 3160], '65': [720], 'Phe': [722], 'Leu': [724], 'position': [726, 3223], '64': [727], 'encoded': [730], 'human-optimized': [735], 'codons': [736], '(7Cormack': [737, 3885], 'B.P.': [738, 3886], 'Valdivia': [739, 3887], 'R.H.': [740, 3888], 'Falkow': [741, 3889], 'Gene': [743, 866, 1903, 3891, 4078], '(Amst.).': [744, 867, 1904, 3892, 4079], '1996;': [745, 761, 783, 822, 838, 3893], '173:': [746, 3894], '33-38Crossref': [747, 3895], '(2489)': [750, 3898], '8Haas': [753], 'Park': [755], 'E.C.': [756], 'Seed': [757], 'B.': [758], 'Curr.': [759], 'Biol.': [760, 1095, 1171, 1221, 1335, 1366, 1488, 1546, 2510], '6:': [762], '315-324Abstract': [763], '(439)': [771], '9Yang': [774], 'T.T.': [775], 'Cheng': [776], 'L.': [777, 1015, 1167, 1325, 1395, 1432, 1478], 'Kain': [778], 'Nucleic': [780], 'Acids': [781], 'Res.': [782], '24:': [784], '4592-4593Crossref': [785], '(348)': [788], 'Crystallographic': [791], 'structures': [792], 'wild-type': [794], 'mutant': [798, 4036], 'S65T': [799], 'reveal': [800], 'tertiary': [804], 'structure': [805, 890, 914], 'resembles': [806], 'barrel': [808], '(10Ormo': [809], 'Cubitt': [811], 'Kallio': [813], 'K.': [814, 1260, 1264], 'Remington': [819], 'S.J.': [820], '273:': [823], '1392-1395Crossref': [824], '(1902)': [827], 'Scholar,11Yang': [829], 'F.': [830], 'L.G.': [832], 'Phillips': [833, 1326, 1479], 'Jr.,': [834], 'G.N.': [835], 'Nature': [836], 'Biotechnol.': [837], '1246-1251Crossref': [840], '(1286)': [843], 'single': [849], 'chain': [850, 1815], 'polypeptide': [851], '238': [853], '(12Prasher': [856], 'Eckenrode': [858], 'V.K.': [859], 'Prendergast': [862], 'F.G.': [863], 'Cormier': [864], 'M.J.': [865], '1992;': [868, 1055, 1096, 1129, 1143, 1156, 1268, 1852, 1998], '111:': [869], '229-233Crossref': [870], '(1748)': [873], 'Most': [876, 1552], 'these': [878, 1183, 1352, 2056, 3118, 3983], 'β': [882], 'sheets': [883], 'are': [885, 1186, 1387, 3122, 3292, 3988, 3996], 'compacted': [886], 'through': [887], 'antiparallel': [889], 'barrel.': [894], 'An': [895], 'α-helix': [896], 'containing': [897, 2412, 2465, 2492], 'located': [901, 3610], 'inside': [902], 'barrel,': [904], 'which': [905, 1114, 1469, 3436, 4301], 'shields': [906], 'from': [908, 1425, 2557, 3548, 3626, 3665, 3976], 'external': [910], 'environment.': [911], 'compact': [913], 'makes': [915, 1696], 'very': [917], 'under': [919, 2121, 2572, 3258, 4296], 'variety': [921], 'conditions,': [923], 'including': [924, 955], 'treatment': [925, 2159, 2218, 2680, 2695, 2777], 'protease': [927], 'some': [953, 979], 'studies,': [954], 'transcriptional': [956, 3453, 3592, 4232], 'studies.Cellular': [958], 'proteins': [959, 968, 1110, 1185, 1196, 1298, 1354, 1526, 1555, 2246, 4107], 'differ': [960], 'their': [963, 3146], 'stabilities.': [964], 'Rapid': [965], 'turnover': [966, 4127, 4140, 4173, 4323], 'often': [970, 1569], 'caused': [971], 'signals': [973], 'induce': [975, 2537, 2616], 'degradation.': [977, 1203, 3010, 4013, 4110], 'In': [978, 1035, 1309, 2721, 3395, 4319], 'cases,': [980, 1037], 'signal': [982, 1039], 'primary': [985, 1349], 'sequence,': [991, 1421], 'possibly': [994], '(13Rogers': [999, 1527], 'Wells': [1001, 1529], 'Rechsteiner': [1003, 1531], '1986;': [1006, 1534], '234:': [1007, 1535], '364-368Crossref': [1008, 1536], '(1946)': [1011, 1539], '14Ghoda': [1014], 'Van': [1016, 1396, 1433], 'Daalen': [1017, 1397, 1434], 'Wetters': [1018, 1398, 1435], 'T.': [1019, 1256, 1262, 1399, 1436, 1463], 'Macrase': [1020, 1400, 1437], 'Ascherman': [1022, 1402, 1439], 'D.': [1023, 1053, 1403, 1440], 'Coffino': [1024, 1091, 1332, 1361, 1404, 1441, 1485, 2506], 'P.': [1025, 1092, 1333, 1362, 1405, 1442, 1486, 2507], '1989;': [1027, 1223, 1243, 1407, 1444, 1905, 4080], '243:': [1028, 1408, 1445], '1493-1495Crossref': [1029, 1409, 1446], '(196)': [1032, 1412, 1449], 'other': [1036, 1713, 4350, 4401], 'modification': [1042, 1122, 1201], 'phosphorylation': [1045], '(15Papavassiliou': [1046], 'A.G.': [1047], 'Treier': [1048], 'Chavrier': [1050], 'C.': [1051, 1219, 1239], 'Bohmann': [1052], '258:': [1056], '1941-1944Crossref': [1057], '(93)': [1060], 'Scholar)': [1062, 2005], 'protein-protein': [1065], 'interaction': [1066], '(16Scheffner': [1067], 'Werness': [1069], 'B.A.': [1070], 'Huibregtse': [1071], 'J.M.': [1072], 'Levine': [1073], 'A.J.': [1074], 'Howley': [1075], 'P.M.': [1076], 'Cell.': [1077, 1094, 2509], '1990;': [1078, 1337, 1490, 1547], '63:': [1079], '1129-1136Abstract': [1080], '(3429)': [1086], '17Li': [1089], 'X.': [1090, 2505], 'Mol.': [1093, 2508], '12:': [1097], '3556-3562Crossref': [1098], '(130)': [1101], 'Prior': [1104], 'degradation,': [1106, 2539], 'most': [1107, 1295], 'short': [1108, 1296, 1524, 1553, 1594, 4105], 'lived': [1109, 1297, 1525, 1554, 1595, 4106], 'need': [1111], 'ubiquitin': [1112, 1121, 1200], 'modification,': [1113], 'catalyzed': [1116], 'number': [1119, 3138, 4024, 4060], 'enzymes': [1123], '(18Goldberg': [1124], 'A.L.': [1125], 'Rock': [1126], 'K.L.': [1127], 'Nature.': [1128, 1267], '357:': [1130], '375-379Crossref': [1131], '(503)': [1134], '19Gottesman': [1137], 'Maurizi': [1139], 'M.R.': [1140], 'Microbiol.': [1141], 'Rev.': [1142, 1154], '56:': [1144], '592-621Crossref': [1145], '20Hershko': [1149], 'Ciechanover': [1151, 1216, 1236], 'Annu.': [1153], '61:': [1157], '761-807Crossref': [1158], '(1192)': [1161], '21Rechsteiner': [1164], 'Hoffman': [1166], 'Dubiel': [1168], 'W.': [1169], 'Chem.': [1172, 1222, 1336, 1367, 1489], '1993;': [1173, 2511], '268:': [1174], '6065-6068Abstract': [1175], 'Only': [1182], 'ubiquitinated': [1184], 'recognized': [1187], 'degraded': [1189, 2974], '26': [1192, 3984], 'proteasome.A': [1194], 'few': [1195], 'do': [1197, 4248], 'One': [1204], '(22Bercovich': [1212], 'Z.': [1213, 1235], 'Rosenberg-Hasson': [1214], 'Y.': [1215, 1233, 1252], 'Kahana': [1218, 1238], '264:': [1224], '15949-15952Abstract': [1225], '23Rosenberg-Hasson': [1232], 'Bercovich': [1234], 'Eur.': [1240], '185:': [1244], '469-474Crossref': [1245], '(73)': [1248], '24Murakami': [1251], 'Matsufuji': [1253], 'Kameji': [1255], 'Hayashi': [1257], 'Igarashi': [1259], 'Tamura': [1261], 'Tanaka': [1263], 'Ichihara': [1265], '360:': [1269], '597-599Crossref': [1270], '(666)': [1273], 'MODC': [1276, 1378, 1426, 1629, 1732, 1789, 2614, 3806, 3966], 'enzyme': [1280], 'biosynthesis': [1283], 'polyamines.': [1285], 'known': [1289], 'one': [1292], 'mammalian': [1300, 1322, 1637, 2502], 'cells,': [1301], 'approximately': [1306, 2992, 3834], '30': [1307, 2109, 2383, 3638], 'min.': [1308, 2110], 'contrast,': [1310, 2722, 3396, 4320], 'Trypanosoma': [1314], 'brucei': [1315, 1464, 2497], 'quite': [1317], 'expressed': [1320, 2556, 2580], 'cells': [1323, 1638, 1941, 1954, 2057, 2067, 2085, 2093, 2134, 2154, 2213, 2327, 2340, 2362, 2372, 2396, 2420, 2449, 2478, 2503, 2637, 2660, 2675, 2737, 2762, 2771, 2793, 2804, 2823, 2839, 2855, 2862, 2874, 2907, 3185, 3252, 3264, 3287, 3339, 3458, 3474, 3484, 3561, 3616, 3742, 3752, 4179, 4186], '(25Ghoda': [1324, 1477], 'M.A.': [1327, 1360, 1480], 'Bass': [1328, 1481], 'K.E.': [1329, 1482], 'Wang': [1330, 1363, 1483], 'C.C.': [1331, 1364, 1484], '265:': [1338, 1491], '11823-11826Abstract': [1339, 1492], 'Comparison': [1346], 'two': [1353, 2828, 3853, 3918], 'shows': [1355, 2587], 'over': [1356, 4135, 4399], '60%': [1357], 'homology': [1358, 1890], '(26Phillips': [1359], '1987;': [1368], '262:': [1369], '8721-8727Abstract': [1370], 'however,': [1377], 'has': [1379, 1588, 3437, 4392], 'extra': [1380], 'C': [1384, 1416, 1602, 1621, 1729, 1781, 1786, 2488, 2611, 3809, 3963], 'terminus': [1385, 1417, 1603, 1622, 1730, 1782, 1787, 2489, 2612, 3810, 3964], 'needed': [1389], 'activity': [1393, 3720], '(14Ghoda': [1394, 1431], 'deletion': [1424], 'prevents': [1427, 4326], 'Furthermore,': [1452, 1662], 'extension': [1455, 1891], 'sufficient': [1458], 'inducing': [1460], 'vivo,': [1468], 'becomes': [1470], 'after': [1472, 2324, 2576, 2634, 2774, 3189, 3368, 3378, 3471, 3556, 3637, 3678, 3708, 3749], 'region': [1476, 1501, 1558, 1567], 'Therefore,': [1499, 2779, 3433, 4088, 4307], 'functions': [1502], '“degradation': [1505], 'domain”': [1506], 'MODC.The': [1508], 'hypothesis': [1509, 4091], 'based': [1518], 'computer': [1521], 'search': [1522], 'among': [1523], '27Rechsteiner': [1542], 'Semin.': [1544], 'Cell': [1545, 4410], '1:': [1548], '433-440PubMed': [1549], 'contain': [1556], 'enriched': [1559], 'Pro,': [1561, 3085, 3196, 3989, 4004], 'Glu,': [1562, 3086, 3197, 3990, 4005], 'Ser,': [1563, 3087, 3198, 3991, 4006], 'Thr.': [1565], 'flanked': [1570], 'basic': [1572, 3098], 'acids,': [1574, 3986], 'Lys,': [1575], 'Arg,': [1576], 'His.': [1578], 'role': [1580], 'subsequently': [1589], 'been': [1590, 3858], 'demonstrated': [1591], 'proteins,': [1596], 'decarboxylase.In': [1607], 'study,': [1609], 'we': [1610, 1863, 2540, 2605, 2723, 2751, 2780, 3083, 3105, 3316, 3531, 3927, 4015, 4191, 4250], 'fused': [1611], 'EGFP.': [1624, 3812, 4136, 4246], 'dramatically': [1630], 'decreased': [1631, 2677, 2701], 'different': [1660, 3498, 3854, 3923, 4104], 'linkage': [1663], 'allowed': [1677], 'TNFα-mediated': [1680, 3342, 3542, 4252], 'HEK293': [1684, 2320, 3560, 3615, 3741], '(dEGFP)': [1691], 'study': [1699, 3451, 4048, 4226], 'real': [1702, 4381], 'living': [1705], 'correlation': [1708, 2890, 3540], 'changes': [1715], 'nuclear': [1718], 'possible.EXPERIMENTAL': [1722], 'PROCEDURESThe': [1723], 'cDNAs': [1724], 'encoding': [1725], 'were': [1733, 1818, 1880, 1886, 1930, 2053, 2068, 2086, 2094, 2113, 2135, 2160, 2169, 2179, 2219, 2226, 2231, 2247, 2282, 2317, 2328, 2341, 2363, 2373, 2397, 2421, 2450, 2479, 2638, 2824, 2840, 2856, 2863, 2912, 3032, 3041, 3058, 3265, 3459, 3475, 3485, 3532, 3562, 3743, 3753, 3789, 4030, 4266, 4276], 'amplified': [1734, 1746, 1791, 1813], 'Pfu': [1736], 'DNA': [1737], 'polymerase': [1738, 1814], '(Stratagene,': [1739], 'Inc.,': [1740, 1944, 2193, 2485], 'La': [1741], 'Jolla,': [1742], 'CA).': [1743, 2196], 'primers': [1748, 1793], 'incorporated': [1750, 1795], 'aSacII': [1751], 'recognition': [1752, 1798], '5′': [1756, 1802], 'aHindIII': [1759], '3′': [1763, 1810], 'end.': [1764, 1811], 'stop': [1766], 'codon': [1767], 'deleted': [1771], 'make': [1773, 2272, 2550], 'continuous': [1775], 'open': [1776, 2880], 'reading': [1777], 'frame': [1778], 'MODC.': [1784], 'HindIII': [1797], 'EcoRI': [1806], 'reaction': [1816], 'products': [1817], 'ligated': [1819], 'theHindIII': [1821], 'site,': [1822], 'cloned': [1827, 1982, 2283], 'into': [1828, 1937, 1983, 2055, 2284, 2319, 2627, 3337], 'pTRE': [1830, 1985, 2027], 'vector': [1832, 1986, 2287], 'tetracycline': [1837], '(Tc)-regulated': [1838], 'system': [1840], '(28Gossen': [1841, 1987], 'Bujard': [1843, 1989], 'H.': [1844, 1990], 'U.': [1849, 1995], '89:': [1853, 1999], '5547-5551Crossref': [1854, 2000], '(4227)': [1857, 2003], 'Using': [1860], 'strategy,': [1862], 'made': [1864, 1887], 'mutants.': [1868], 'Key': [1869], 'Ala.': [1883, 3103], 'using': [1888, 2058, 2185, 2207, 2249, 3253, 4199], 'procedure': [1892, 3255], '(33Horton': [1893, 4068], 'R.M.': [1894, 4069], 'Hunt': [1895, 4070], 'H.D.': [1896, 4071], 'Ho': [1897, 4072], 'S.N.': [1898, 4073], 'Pullen': [1899, 4074], 'J.K.': [1900, 4075], 'Peasse': [1901, 4076], 'L.R.': [1902, 4077], '77:': [1906, 4081], '61-68Crossref': [1907, 4082], '(2629)': [1910, 4085], 'include': [1915], 'P426A/P427A,': [1916, 1925], 'P438A,': [1917], 'E428A/E430A/E431A,': [1918], 'E444A,': [1919], 'S440A,': [1920], 'S445A,': [1921], 'T436A,': [1922], 'D433A/D434A,': [1923], 'D448A,': [1924], 'P438A.The': [1927], 'construct': [1928, 2554, 2622, 3333], 'DNAs': [1929, 2052], 'purified': [1931], 'Qiagen': [1933], 'columns': [1934], 'CHO': [1938, 1951, 2582, 2628, 2820, 3241], 'K1': [1939, 1952, 2583, 2629, 2821, 3242], 'Tet-off': [1940, 1953, 2584, 2630, 2822, 3243], '(CLONTECH': [1942, 2006, 2060, 2255, 2269], 'Laboratories,': [1943, 2007, 2061, 2256, 2270], 'Palo': [1945], 'Alto,': [1946], 'CA)': [1947], 'stably': [1955, 4188], 'express': [1956], 'tet': [1962], 'repressor': [1963, 2022], 'herpes': [1966, 2290], 'simplex': [1967, 2291], 'virus': [1968, 2292], 'VP16': [1969], '(tetracycline-controlled': [1970], 'transactivator)': [1971], 'thus': [1973], 'Tc-regulated': [1978, 2559], 'genes': [1981], 'Inc.).': [2008, 2062, 2257], 'Tetracycline-controlled': [2009], 'transactivator': [2010], 'initiates': [2011], 'modified': [2017], 'cytomegalovirus': [2018], 'promoter': [2019], 'Tet': [2021], 'elements': [2024, 4230, 4352], 'vector.': [2028], 'blocked': [2033], 'controlled': [2042], 'level': [2045, 2945, 3424, 3938, 4299], 'Tc': [2047], 'medium.': [2050], 'These': [2051, 2315, 3191, 4361], 'introduced': [2054], 'CLONfectin': [2059], 'After': [2063, 2090, 2367, 2442, 2470, 2692, 2834, 3261, 3648], '24': [2064, 2100, 2835, 3262, 3469], 'h,': [2065, 2650, 2836, 3263, 3370, 3375, 3650], 'subject': [2069, 3042], 'functional': [2071], 'analyses': [2072], 'described': [2074, 3257], 'below.To': [2075], 'examine': [2076, 2352, 2888, 3538], 'intensity': [2079, 2184, 2565, 2591, 2656, 2668, 2699, 2733, 3113, 3279, 3505], 'EGFP-MODC-(422–461),': [2083, 3236], 'cultured': [2087], 'coverslips.': [2089], 'transfection,': [2091, 2325, 2635, 3472, 3750], 'incubated': [2095, 2422, 2451], '37': [2097], '°C': [2098], 'h': [2101, 2977, 3188, 3274, 3470, 3572, 3748], 'then': [2103, 2180, 2302, 2398, 3582], 'fixed': [2104, 2374, 2395], '4%': [2106, 2376, 2406, 2466], 'paraformaldehyde': [2107, 2151, 2377], 'coverslips': [2112], 'mounted': [2114, 2480], 'glass': [2117], 'slide': [2118], 'examined': [2120, 2571, 2662, 3767], 'Zeiss': [2123], 'Axioskop': [2124], 'model': [2125], '50': [2126], 'microscope.': [2128], 'To': [2129, 2529, 2740, 3305, 4247], 'determine': [2130, 2530, 2741, 3306], 'turnover,': [2132, 3440, 4290], 'treated': [2136, 2329, 2639, 2841, 2854, 3266, 3476, 3563, 3754], '(CHX)': [2139], 'final': [2142], 'concentration': [2143], '100': [2145, 2641, 2843], 'μg/ml': [2146, 2332, 2642, 2844, 3480], 'varying': [2148, 3211], 'times': [2149, 2473], 'before': [2150, 3617], 'fixation.The': [2152], 'without': [2157, 2216, 4383], 'CHX': [2158, 2217, 2643, 2679, 2845, 2966, 2979, 3268], 'collected': [2161, 2220, 2342, 2857, 3028, 3486], 'EDTA': [2163], 'treatment,': [2164, 2369, 4332], 'pellets': [2168], 'resuspended': [2170], '0.5': [2172], 'ml': [2173], 'PBS.': [2175], 'suspensions': [2178], 'analyzed': [2181, 3276], 'FACS': [2187, 2866, 3281], 'calibur': [2188], 'flow': [2189, 2349, 2753, 2809, 2910, 3030, 3492, 3825], 'cytometer': [2190], '(Becton': [2191, 3283], 'Dickinson,': [2192], 'San': [2194], 'Jose,': [2195], 'excited': [2199], '488': [2201], 'nm,': [2202], 'emission': [2204, 3520], '510/20': [2209], 'bandpass': [2210], 'filter.The': [2211], 'lysates': [2225, 3040], 'prepared': [2227], 'sonication.': [2229], 'Proteins': [2230], 'separated': [2232], 'SDS': [2234, 3044], 'gel': [2235, 3045], 'electrophoresis,': [2236], 'transferred': [2237, 3048], 'onto': [2238, 3049], 'membrane,': [2240], 'EGFP-MODC': [2244, 2746, 3119], 'monoclonal': [2251, 2921, 3062], 'antibody': [2252, 2922, 2930, 3063], 'against': [2253, 2428, 2923, 3064, 3578, 3770], 'visualized': [2261], 'Exposure': [2265], 'chemiluminescent': [2266], 'kit': [2268], 'Inc.).To': [2271], 'TNFα-responding': [2274], 'vector,': [2275, 2561], '4': [2276, 3273, 3320], 'copies': [2277, 3321], 'pSEAP2': [2286], 'thymidine': [2293, 3329], 'kinase': [2294, 3330], 'promoter,': [2295], 'making': [2296, 2311], 'pNFκB-SEAP.': [2297], 'SEAP': [2299, 2344, 3364, 3488], 'replaced': [2303], 'dEGFP': [2308, 3308, 3366, 3384, 3597, 3641, 3661, 3675, 3728, 3781, 3788, 3832, 3959, 4129, 4142, 4238, 4262, 4325, 4341, 4365, 4375], '(EGFP-MODC-(422–461))': [2309], 'gene,': [2310], 'pNFκB-EGFP': [2312], 'pNFκB-dEGFP.': [2314, 2366, 3468, 3746], 'constructs': [2316, 2829], 'Twenty-four': [2322, 2632], 'hours': [2323, 2633, 3555], '0.1': [2331, 3479], 'recombinant': [2333], 'human': [2334, 3481], 'TNFα.': [2335, 3482], 'medium': [2337], 'assaying': [2345], '(CLONTECHLaboratories,': [2346], 'Inc.)': [2347], 'cytometric': [2350, 2810], 'analysis.To': [2351], 'both': [2353, 3363, 3911], 'NFκB-mediated': [2357, 3737, 3776], '293': [2361], 'TNF': [2368, 3756], 'PBS': [2379, 2391, 2411, 2445, 2464], '(pH': [2380], '7.4)': [2381], 'min': [2384, 2462, 3884], 'room': [2386], 'temperature': [2387], 'rinsed': [2389], 'three': [2392, 2446, 2472, 4133], 'times.': [2393], 'permeabilized': [2399], 'blocking': [2402, 2437], 'solution': [2403, 2438], 'consisting': [2404], 'bovine': [2407, 2467], 'serum': [2408, 2468], 'albumin': [2409], '0.1%': [2413], 'Triton': [2414], 'X-100': [2415], '1': [2417, 4003], '1:250': [2424, 2453], 'diluted': [2425, 2454], 'polyclonal': [2426, 3576], 'antibodies': [2427, 3577, 3769], 'p65': [2430, 3580, 3771], '(Upstate': [2431], 'Biotechnology,': [2432], 'Lake': [2433], 'Placid,': [2434], 'NY)': [2435], 'washing': [2443], 'times,': [2447], 'rhodamine-labeled': [2455, 3773], 'anti-rabbit': [2456], 'IgG': [2457, 3774], '(Boehringer': [2458], 'Mannheim)': [2459], '45': [2461], 'albumin.': [2469], 'rinsing': [2471], 'stained': [2477, 3574], 'Citifluor': [2482], '(Ted': [2483], 'Pella,': [2484], 'Redding,': [2486], 'CA).RESULTSThe': [2487], '422–461,': [2495], 'inducesT.': [2496], '(31Li': [2504], '13:': [2512], '2377-2383Crossref': [2513], '(154)': [2516], 'fragment': [2520], '423–449.': [2528], 'whether': [2531, 3307], 'appended': [2541], 'EGFP-MODC-(422–461).': [2551, 2819, 3026], 'pTRE.': [2562], 'microscope': [2575], 'transiently': [2579, 2625, 3335], 'Fig.1': [2586], 'no': [2599], 'difference': [2600, 3848], 'intracellular': [2603], 'distribution.Next,': [2604], 'ability': [2608], 'vivo.': [2620], 'first': [2624], '0,': [2645, 2847, 3270, 3567, 3758], '1,': [2646, 2848], '2,': [2647, 2849, 3271, 3569, 3760], '3': [2649, 2719, 2851, 3995], 'change': [2653, 2729, 2758, 2942, 3110], 'microscopy.': [2665], 'gradually': [2676], 'extended': [2682], '(Fig.': [2683, 2738], '1)': [2684], 'indicating': [2685], 'unstable.': [2691], '3-h': [2694], 'more': [2703, 2749, 2796, 3950, 4032, 4093], 'than': [2704, 2718, 2797, 3940, 4315], '50%.': [2705], 'results': [2707, 2996, 3192, 3381, 3682, 4174, 4333, 4362], 'suggest': [2708], 'less': [2717, 4176], 'observe': [2726], 'significant': [2728], 'EGFP-transfected': [2736, 2792], '1).': [2739], 'accurately,': [2750], 'cytometry': [2754, 2911, 3031, 3493, 3826], 'measure': [2756], 'expressing': [2763], 'Half': [2767, 2968], 'untreated': [2770, 2803], 'maintained': [2772], '2-h': [2776], '(Fig.2).': [2778], 'conclude': [2781, 2982, 3954], 'still': [2794], '90%': [2798], 'relative': [2801], 'during': [2805, 3029, 3412, 3645], 'treatment.Figure': [2807], '2A,': [2808], 'stabilities': [2815, 3022], 'Fig.': [2832], '1.': [2833], 'EDTA,': [2859], '10,000': [2861], 'subjected': [2864], 'analysis.': [2867], 'B,': [2868], 'percentage': [2870, 3296], 'plotted.': [2876], 'Solid': [2877], 'circles,': [2878, 2881], 'EGFP;': [2879], 'EGFP-MODC-(422–461).View': [2882], 'Large': [2883, 3066, 3227, 3508, 3793], 'Image': [2884, 3067, 3228, 3509, 3794], 'Figure': [2885, 3068, 3229, 3510, 3795], 'ViewerDownload': [2886, 3069, 3230, 3511, 3796], '(PPT)To': [2887, 3070], 'between': [2891, 3541], 'GFP.': [2924, 3868, 3915], 'As': [2925, 3528, 3588], 'shown': [2926, 3123, 3589], 'Fig.3,': [2928], 'able': [2932, 3533, 4344], 'detect': [2934], 'No': [2940], 'detectable': [2941], 'found.': [2949], '31': [2956], 'kDa': [2957], 'unstable,': [2959, 4033], 'measured': [2961], 'decline': [2964], 'following': [2965, 4330], 'treatment.': [2967, 2980, 3190, 3414, 3619], 'within': [2975], '1–2': [2993, 3845], 'Our': [2995], 'indicate': [2997, 4363], 'correlates': [3006], 'designated': [3015], 'dEGFP.Figure': [3016], '3Western': [3017], 'Cells': [3027], 'preparing': [3035], 'lysates.': [3037], 'electrophoresis': [3046], 'membrane.': [3051], 'GFP.View': [3065], 'evaluate': [3071], 'contribution': [3073], 'residues': [3090, 3141, 3170, 3201, 4063], 'well': [3094, 3409], 'flanking': [3097], 'residues,': [3101], 'Next,': [3104], 'monitored': [3106, 3595, 4251], '(Fig.4).': [3114], 'data': [3116], 'Table': [3125], 'I.': [3126], 'found': [3128, 4016], 'rate': [3132], 'proportional': [3135], 'but': [3142, 3165, 3640, 3907], 'determined': [3144, 3779], 'positions,': [3147], 'Glu': [3151, 3156, 3169], 'mutations.': [3152], 'Mutation': [3153, 3998], 'residue': [3157], '444': [3161], 'stabilized': [3162], 'mutation': [3166], '428,': [3174], '430,': [3175], '431': [3177], 'shortened': [3178], 'half-life;': [3180], 'only': [3181], '20%': [3182], 'fluoresced': [3186], 'suggested': [3193], 'contribute': [3206], 'instability': [3209, 4054], 'ways.Figure': [3212], '4Schematic': [3213], 'map': [3214], 'mutations.View': [3226], '(PPT)Table': [3231], 'IFACS': [3232], 'cellsConstructs0': [3244], 'hInitial2': [3245], 'h4': [3246], 'h%%%EGFP100(63.6)10792EGFP-MODC-(422–461)100(12.6)5229P426A/P427A100(11.5)3911P438A100(34.1)7960E428A/E430A/E431A100(17.3)2015E444A100(12.6)6965S440A100(21.6)7866S445A100(23.5)2920T436A100(46.9)7047D433A/D434A100(11.31)226D448A100(32.6)3015H423A100(12.2)5025R449A/H450A100(27.9)9386Transfection': [3247], 'performed': [3249], 'CHO/tTA': [3251], '“Experimental': [3259], 'Procedures.”': [3260], 'caliber': [3282], 'Dickinson).': [3284], 'each': [3289], 'point': [3291], 'represented': [3293], 'initial.': [3298], 'Open': [3299], 'table': [3300], 'new': [3303], 'tab': [3304], 'reporter,': [3315], 'linked': [3317], 'constitutive': [3328], 'promoter.': [3331], 'HeLa': [3338], 'activation': [3344], '(Fig.5).': [3345], 'another': [3356], 'commonly': [3357], 'genetic': [3359, 4201], 'Induction': [3361], 'occurred': [3367], 'maximized': [3372], '6': [3374, 3571, 3679, 3709, 3762], 'declined': [3377], 'that.': [3379], 'demonstrate': [3382, 3683], 'monitor': [3392], 'induction.': [3394, 4306], 'induction,': [3405], 'respond': [3408], 'TNFα': [3411, 3501, 3565, 3618, 3620, 3694, 4331], 'might': [3417], 'because': [3419, 3859, 4286], 'higher': [3422], 'basal': [3423, 4298], 'accumulating': [3429], 'cannot': [3443], 'induction.Figure': [3454], '5TNFα-mediated': [3455], 'activation.HEK293': [3457], 'constructs,': [3464], 'pNFκB-SEAP,': [3465], 'pNFκB-EGFP,': [3466], 'either': [3477], 'assay': [3489], '(A)': [3490], '(B)': [3495], 'doses': [3499], '(C).': [3502], 'Normalized': [3503], 'presented.View': [3507], '(PPT)dEGFP,': [3512], 'like': [3513], 'autofluorescent': [3517], 'whose': [3519], 'cofactors': [3525], 'substrates.': [3527], 'result,': [3530], 'cytoplasm': [3550, 3613, 3628, 3707], 'Sixteen': [3554], 'transfection': [3557], 'pNFκB-dEGFP,': [3559], '0.5,': [3568, 3759], 'rhodamine-conjugated': [3585], 'secondary': [3586], 'antibody.': [3587], 'Fig.6,': [3591], '(green)': [3598], 'process': [3602, 3633], 'rhodamine': [3606], '(red).': [3607], 'induced': [3621], 'nearly': [3635], 'complete': [3636], 'min,': [3639], 'period.': [3647], 'accumulated': [3653, 4294], 'nucleus,': [3656], 'lessened.': [3662], 'relocated': [3664], 'nucleus': [3667], 'cytoplasm,': [3670], 'highest': [3677], 'transient': [3690, 3712, 3719, 4222], 'all': [3697], 'translocated': [3700], 'recruited': [3703], 'back': [3704], 'resulted': [3716, 4039], 'mediation': [3723], 'SEAP.Figure': [3730], '6Simultaneous': [3731], 'dEGFP.': [3740, 4318], '16': [3747], '(A).': [3775], '(B).': [3782], 'simultaneously': [3791], '(C).View': [3792], '(PPT)DISCUSSIONWe': [3797], 'appending': [3801], 'CHX.': [3822], 'Analysis': [3823], 'methods': [3855, 3919], 'may': [3856], 'have': [3857], 'nonfluorescent': [3864], 'premature': [3865, 3912], 'mature': [3867, 3905, 3914], 'Maturation': [3869], 'post-translational': [3875], 'proceeds': [3877], 'half-time': [3880], 'about': [3882], '25': [3883], 'Fluorescence': [3901], 'measurement': [3902], 'detects': [3903, 3910], 'solely': [3904], 'blotting': [3909], 'Thus,': [3916, 4190], 'will': [3920, 4208], 'determinations.': [3925], 'wish': [3928], 'rather': [3939], 'level,': [3944], 'important': [3951], 'quality.': [3952], 'h.The': [3962], 'induces': [3968], 'vivocontains': [3972], '423': [3979], '449.': [3981], 'Of': [3982], '10': [3987], 'Asp.': [3997], 'indicates': [4000], 'least': [4002, 4132], 'required': [4010], 'residues.': [4027], 'Some': [4028], 'even': [4031, 4295], 'S445A.': [4037], 'conflict': [4042], 'those': [4044, 4269], 'observed': [4045], 'yeast': [4050], 'uracil': [4051], 'permease': [4052], 'where': [4053], 'total': [4059], 'PEST-like': [4066], 'needs': [4092], 'validation': [4094], 'probably': [4095], 'determining': [4097], 'contributions': [4099], 'detected,': [4115], 'excellent': [4119], 'degradation.The': [4125], 'provides': [4130], 'advantages': [4134], 'First,': [4137], 'requiring': [4147], 'circadian': [4152], 'rhythms': [4153], '(32Abrams': [4155], 'S.B.': [4156], 'Circadian': [4157], 'Rhythms': [4158], "Aren't": [4159], 'All': [4160], 'Head.': [4163], 'Biophotonics': [4164], 'International,': [4165], 'Pittsfield,': [4166], 'MA1998:': [4167], '24-25Google': [4168], 'Second,': [4170], 'leading': [4180], 'lower': [4182], 'toxicity': [4183], 'expressed.': [4189], 'believe': [4192], 'establishing': [4194], 'line': [4198], 'place': [4204], 'easier.': [4210], 'Finally,': [4211], 'version': [4214], 'cis-acting': [4228, 4351], 'regulatory': [4229], 'induction.We': [4233], 'compared': [4234], 'utility': [4236, 4369], 'so,': [4249], 'SEAP;': [4271], 'not.': [4277], 'likely': [4279], 'explanation': [4280], 'phenomenon': [4283], 'slow': [4289], 'noninduced': [4297], 'expression,': [4300], 'narrows': [4302], 'range': [4304], 'much': [4313], 'slower': [4314], 'and,': [4329], 'SEAP.': [4340], 'show': [4346], 'stimulated': [4353], 'cAMP,': [4355], 'dexamethasone,': [4356], 'etc.': [4357], '(data': [4358], 'shown).': [4360], 'general': [4368], 'addition': [4385], 'substrates': [4388], 'cofactors,': [4390], 'obvious': [4394], 'advantage': [4395], 'cell-based': [4397], 'assays': [4398], 'reporters,': [4402], 'firefly': [4406], 'luciferase,': [4407], 'β-galactosidase.': [4409], 'lines': [4411]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2068962163', 'counts_by_year': [{'year': 2024, 'cited_by_count': 34}, {'year': 2023, 'cited_by_count': 37}, {'year': 2022, 'cited_by_count': 38}, {'year': 2021, 'cited_by_count': 33}, {'year': 2020, 'cited_by_count': 46}, {'year': 2019, 'cited_by_count': 44}, {'year': 2018, 'cited_by_count': 35}, {'year': 2017, 'cited_by_count': 26}, {'year': 2016, 'cited_by_count': 39}, {'year': 2015, 'cited_by_count': 31}, {'year': 2014, 'cited_by_count': 37}, {'year': 2013, 'cited_by_count': 32}, {'year': 2012, 'cited_by_count': 29}], 'updated_date': '2024-12-11T07:09:56.407578', 'created_date': '2016-06-24'}
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