Title: Effects of aminooxyacetate, alanine and other amino acids on protein synthesis in isolated rat hepatocytes
Abstract: Protein synthesis in isolated rat hepatocytes has been measured by the incorporation of [14C]valine at high concentration and constant specific activity (5 mmol/l and 315 μCi/l). Protein synthesis was stimulated by the addition of an amino acid mixture, and by a number of individual amino acids alone, most notably alanine. Energy substrates (lactate, pyruvate) stimulated protein synthesis to the same extent as alanine, suggesting that a major part of the amino acid effect could be due to the provision of energy. Aminooxyacetate, an inhibitor of glutamate transaminases, inhibited protein synthesis strongly (70%), and abolished the stimulatory effects of alanine and energy substrates. This could indicate that hepatocytic protein synthesis is subject to positive control by a transamination-dependent agent.
Publication Year: 1978
Publication Date: 1978-10-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 55
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