Title: Alkali-labile colicinogenic factor E1 DNA molecules formed in the presence of N-methyl-N′-nitro-N-nitrosoguanidine
Abstract: Plasmid ColE1 DNA of E. coli was used as a target DNA molecule to analyse the structural modification of DNA by N-methyl-N′-nitro-N-nitrosoguanidine. When the low concentration of this drug was used, both cell growth and overall DNA synthesis were neither stimulated nor inhibited, and plasmid DNA molecules were isolated as closed circles after replication. These molecules were stable for the ribonuclease treatment, but became susceptible to the alkaline hydrolysis. Such alkali-labile sites of ColE1 DNA were found in the parental strands and randomly distributed from the restriction endonuclease EcoR1 cleavage site.
Publication Year: 1977
Publication Date: 1977-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 11
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