Title: VirD2 Protein of <i>Agrobacterium tumefaciens</i> Very Tightly Linked to the 5' End of T-Strand DNA
Abstract: The T-strand, a probable intermediate of Agrobacterium plant transformation, is bound by a nondenaturable linkage to a protein moiety at its 5′ end. The protein is shown to be the polypeptide VirD2, previously identified as a component of the T-DNA border endonuclease that initiates T-strand production. T-strands from an Agrobacterium strain expressing a virD2-lacZ fusion are bound to a protein of larger size than the wild-type protein and are immunoprecipitable by antibody to β-galactosidase.
Publication Year: 1988
Publication Date: 1988-11-11
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 139
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