Title: Regulation and Homologous Expression of PNA in <i>Panax ginseng</i> by RNAI
Abstract: Panax ginseng (C.A.Meyer), belonging to Araliaceae family Panax category, has important commercial value and are used in worldwide. Ginsenosides, the major bioactive ingredients of P. ginseng , are classified into two groups by the skeleton of aglycones, namely dammarane-type and oleanane-type. Dammarane-type ginsenosides dominate over oleanane-type not only in amount but also in structural varieties. Here, we cloned and identified the Dammarenediol-II synthase gene named PNA encoding encoding enzymes catalyzed 2,3-oxidosqualene to dammarendediol-II by RT-PCT.we established PNA RNAi transgenic. The RT-PCT and HPLC analysis of the final products of protopanaxadiol-type and protopanaxatrio-type ginsenosides showed a result that declined level of protopanaxadiol-type and protoanaxatriol-type ginsenosides, and increased phytosterol content.
Publication Year: 2013
Publication Date: 2013-09-01
Language: en
Type: article
Indexed In: ['crossref']
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