Publication Information

{'id': 'https://openalex.org/W2066078187', 'doi': 'https://doi.org/10.1002/jemt.20431', 'title': 'Multi-dimensional fluorescence lifetime and FRET measurements', 'display_name': 'Multi-dimensional fluorescence lifetime and FRET measurements', 'publication_year': 2007, 'publication_date': '2007-03-28', 'ids': {'openalex': 'https://openalex.org/W2066078187', 'doi': 'https://doi.org/10.1002/jemt.20431', 'mag': '2066078187', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/17393489'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/jemt.20431', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S6191820', 'display_name': 'Microscopy Research and Technique', 'issn_l': '1059-910X', 'issn': ['1059-910X', '1097-0029'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5061725835', 'display_name': 'Christoph Biskup', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I193291145', 'display_name': 'Schiller International University', 'ror': 'https://ror.org/003tmp461', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I193291145']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Christoph Biskup', 'raw_affiliation_strings': ['Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany', 'institution_ids': ['https://openalex.org/I193291145']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5101452650', 'display_name': 'Thomas Zimmer', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I193291145', 'display_name': 'Schiller International University', 'ror': 'https://ror.org/003tmp461', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I193291145']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Thomas Zimmer', 'raw_affiliation_strings': ['Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany', 'institution_ids': ['https://openalex.org/I193291145']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5076646886', 'display_name': 'Laimonas Kelbauskas', 'orcid': 'https://orcid.org/0000-0002-9035-1642'}, 'institutions': [{'id': 'https://openalex.org/I193291145', 'display_name': 'Schiller International University', 'ror': 'https://ror.org/003tmp461', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I193291145']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Laimonas Kelbauskas', 'raw_affiliation_strings': ['Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany', 'institution_ids': ['https://openalex.org/I193291145']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5113776301', 'display_name': 'Birgit Hoffmann', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I193291145', 'display_name': 'Schiller International University', 'ror': 'https://ror.org/003tmp461', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I193291145']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Birgit Hoffmann', 'raw_affiliation_strings': ['Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany', 'institution_ids': ['https://openalex.org/I193291145']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5038091996', 'display_name': 'Nikolaj Klöcker', 'orcid': 'https://orcid.org/0000-0002-7864-1348'}, 'institutions': [{'id': 'https://openalex.org/I161046081', 'display_name': 'University of Freiburg', 'ror': 'https://ror.org/0245cg223', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I161046081']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Nikolaj Klöcker', 'raw_affiliation_strings': ['Institut für Physiologie II, Albert‐Ludwigs‐Universität Freiburg, 79104 Freiburg, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Physiologie II, Albert‐Ludwigs‐Universität Freiburg, 79104 Freiburg, Germany', 'institution_ids': ['https://openalex.org/I161046081']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5103385655', 'display_name': 'Wolfgang Becker', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210104073', 'display_name': 'Becker Technologies (Germany)', 'ror': 'https://ror.org/01hv3m305', 'country_code': 'DE', 'type': 'company', 'lineage': ['https://openalex.org/I4210104073']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Wolfgang Becker', 'raw_affiliation_strings': ['Becker & Hickl GmbH, Nahmitzer Damm 30, 12277 Berlin, Germany'], 'affiliations': [{'raw_affiliation_string': 'Becker & Hickl GmbH, Nahmitzer Damm 30, 12277 Berlin, Germany', 'institution_ids': ['https://openalex.org/I4210104073']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5108437719', 'display_name': 'Axel Bergmann', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210104073', 'display_name': 'Becker Technologies (Germany)', 'ror': 'https://ror.org/01hv3m305', 'country_code': 'DE', 'type': 'company', 'lineage': ['https://openalex.org/I4210104073']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Axel Bergmann', 'raw_affiliation_strings': ['Becker & Hickl GmbH, Nahmitzer Damm 30, 12277 Berlin, Germany'], 'affiliations': [{'raw_affiliation_string': 'Becker & Hickl GmbH, Nahmitzer Damm 30, 12277 Berlin, Germany', 'institution_ids': ['https://openalex.org/I4210104073']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5013747364', 'display_name': 'Klaus Benndorf', 'orcid': 'https://orcid.org/0000-0002-0707-4083'}, 'institutions': [{'id': 'https://openalex.org/I193291145', 'display_name': 'Schiller International University', 'ror': 'https://ror.org/003tmp461', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I193291145']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Klaus Benndorf', 'raw_affiliation_strings': ['Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany'], 'affiliations': [{'raw_affiliation_string': 'Institut für Physiologie II, Friedrich-Schiller-Universität, Teichgraben 8, 07740 Jena, Germany', 'institution_ids': ['https://openalex.org/I193291145']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 3, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 3860, 'currency': 'USD', 'value_usd': 3860, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 3.826, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 78, 'citation_normalized_percentile': {'value': 0.999436, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 96, 'max': 97}, 'biblio': {'volume': '70', 'issue': '5', 'first_page': '442', 'last_page': '451'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10540', 'display_name': 'Advanced Fluorescence Microscopy Techniques', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1304', 'display_name': 'Biophysics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10540', 'display_name': 'Advanced Fluorescence Microscopy Techniques', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1304', 'display_name': 'Biophysics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T13127', 'display_name': 'Biotin and Related Studies', 'score': 0.9973, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12867', 'display_name': 'Advanced Biosensing Techniques and Applications', 'score': 0.9923, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/acceptor', 'display_name': 'Acceptor', 'score': 0.8039359}, {'id': 'https://openalex.org/keywords/fluorometer', 'display_name': 'Fluorometer', 'score': 0.47630608}], 'concepts': [{'id': 'https://openalex.org/C96305047', 'wikidata': 'https://www.wikidata.org/wiki/Q367473', 'display_name': 'Förster resonance energy transfer', 'level': 3, 'score': 0.9565128}, {'id': 'https://openalex.org/C2779892579', 'wikidata': 'https://www.wikidata.org/wiki/Q912138', 'display_name': 'Acceptor', 'level': 2, 'score': 0.8039359}, {'id': 'https://openalex.org/C91881484', 'wikidata': 'https://www.wikidata.org/wiki/Q191807', 'display_name': 'Fluorescence', 'level': 2, 'score': 0.7769004}, {'id': 'https://openalex.org/C62535639', 'wikidata': 'https://www.wikidata.org/wiki/Q15055422', 'display_name': 'Fluorescence in the life sciences', 'level': 4, 'score': 0.6738417}, {'id': 'https://openalex.org/C121745418', 'wikidata': 'https://www.wikidata.org/wiki/Q585536', 'display_name': 'Quenching (fluorescence)', 'level': 3, 'score': 0.5849657}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.563294}, {'id': 'https://openalex.org/C202800777', 'wikidata': 'https://www.wikidata.org/wiki/Q1781297', 'display_name': 'Fluorometer', 'level': 3, 'score': 0.47630608}, {'id': 'https://openalex.org/C75473681', 'wikidata': 'https://www.wikidata.org/wiki/Q188651', 'display_name': 'Photochemistry', 'level': 1, 'score': 0.41686714}, {'id': 'https://openalex.org/C120665830', 'wikidata': 'https://www.wikidata.org/wiki/Q14620', 'display_name': 'Optics', 'level': 1, 'score': 0.20430702}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.13605982}, {'id': 'https://openalex.org/C26873012', 'wikidata': 'https://www.wikidata.org/wiki/Q214781', 'display_name': 'Condensed matter physics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D003952', 'descriptor_name': 'Diagnostic Imaging', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D031541', 'descriptor_name': 'Fluorescence Resonance Energy Transfer', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D011506', 'descriptor_name': 'Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D002460', 'descriptor_name': 'Cell Line', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003952', 'descriptor_name': 'Diagnostic Imaging', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018613', 'descriptor_name': 'Microscopy, Confocal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011485', 'descriptor_name': 'Protein Binding', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011506', 'descriptor_name': 'Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013997', 'descriptor_name': 'Time Factors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014162', 'descriptor_name': 'Transfection', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/jemt.20431', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S6191820', 'display_name': 'Microscopy Research and Technique', 'issn_l': '1059-910X', 'issn': ['1059-910X', '1097-0029'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/17393489', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'score': 0.86, 'id': 'https://metadata.un.org/sdg/7', 'display_name': 'Affordable and clean energy'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 32, 'referenced_works': ['https://openalex.org/W1584704387', 'https://openalex.org/W1644749979', 'https://openalex.org/W1781343620', 'https://openalex.org/W1971276614', 'https://openalex.org/W1974863459', 'https://openalex.org/W1986804563', 'https://openalex.org/W1989579457', 'https://openalex.org/W1992489841', 'https://openalex.org/W1999814206', 'https://openalex.org/W2014293907', 'https://openalex.org/W2032579724', 'https://openalex.org/W2035726898', 'https://openalex.org/W2065865945', 'https://openalex.org/W2068281228', 'https://openalex.org/W2068924803', 'https://openalex.org/W2076815583', 'https://openalex.org/W2086247286', 'https://openalex.org/W2090663204', 'https://openalex.org/W2100320779', 'https://openalex.org/W2103364571', 'https://openalex.org/W2127068865', 'https://openalex.org/W2127230663', 'https://openalex.org/W2134153749', 'https://openalex.org/W2141652419', 'https://openalex.org/W2143094150', 'https://openalex.org/W2167373328', 'https://openalex.org/W2290450005', 'https://openalex.org/W2335552270', 'https://openalex.org/W2795480261', 'https://openalex.org/W323936032', 'https://openalex.org/W33325562', 'https://openalex.org/W995681685'], 'related_works': ['https://openalex.org/W2797913390', 'https://openalex.org/W2368470629', 'https://openalex.org/W2142891272', 'https://openalex.org/W2112976975', 'https://openalex.org/W2079357620', 'https://openalex.org/W2048074445', 'https://openalex.org/W2004925055', 'https://openalex.org/W1988949448', 'https://openalex.org/W1987066967', 'https://openalex.org/W1599075286'], 'abstract_inverted_index': {'When': [0, 48], 'and': [1, 45, 53, 82, 116, 148, 173, 223], 'where': [2], 'proteins': [3, 37, 50], 'associate': [4], 'with': [5, 42], 'each': [6], 'other': [7, 120, 175, 207], 'in': [8, 14, 111, 125, 164, 193, 230], 'living': [9], 'cells': [10], 'are': [11, 56, 168], 'key': [12], 'questions': [13, 24], 'many': [15], 'biological': [16], 'research': [17], 'projects.': [18], 'One': [19, 106, 132, 197], 'way': [20], 'to': [21, 26, 73, 102, 108, 122, 144, 171, 219], 'address': [22], 'these': [23], 'is': [25, 107, 121, 140, 199, 209], 'measure': [27, 123], 'the': [28, 63, 74, 80, 83, 86, 91, 94, 112, 119, 126, 129, 136, 165, 194, 206, 228], 'extent': [29], 'of': [30, 69, 85, 93, 114, 128, 135], 'Förster': [31], 'resonance': [32], 'energy': [33, 72], 'transfer': [34], '(FRET)': [35], 'between': [36, 146], 'that': [38, 62, 141, 162], 'have': [39], 'been': [40], 'labeled': [41], 'appropriate': [43], 'donor': [44, 52, 64, 87, 115, 130, 150, 222], 'acceptor': [46, 54, 95, 117, 224], 'fluorophores.': [47], 'both': [49, 79, 221], 'interact,': [51], 'fluorophores': [55], 'brought': [57], 'into': [58], 'close': [59], 'vicinity': [60], 'so': [61], 'can': [65, 184], 'transmit': [66], 'a': [67, 77, 202, 212, 231], 'part': [68], 'its': [70], 'excitation': [71], 'acceptor.': [75], 'As': [76], 'result,': [78], 'intensity': [81, 92, 113, 154], 'lifetime': [84, 127, 138, 191], 'fluorescence': [88, 137, 190, 225], 'decrease,': [89], 'whereas': [90], 'emission': [96], 'increases.': [97], 'This': [98], 'offers': [99], 'different': [100], 'approaches': [101, 216], 'determine': [103], 'FRET': [104, 172], 'efficiency:': [105], 'detect': [109], 'changes': [110, 124, 163], 'emission,': [118], 'molecule.': [131], 'important': [133], 'advantage': [134], 'approach': [139], 'it': [142, 156], 'allows': [143], 'distinguish': [145], 'free': [147], 'associated': [149], 'molecules.': [151], 'However,': [152], 'like': [153], 'measurements': [155, 192], 'lacks': [157], 'an': [158], 'intrinsic': [159], 'control': [160], 'ensuring': [161], 'measured': [166], 'parameters': [167], 'only': [169], 'due': [170], 'not': [174], 'quenching': [176], 'processes.': [177], 'Here,': [178], 'we': [179], 'show': [180], 'how': [181], 'this': [182], 'limitation': [183], 'be': [185], 'overcome': [186], 'by': [187, 227], 'spectrally': [188], 'resolved': [189], 'time': [195], 'domain.': [196], 'technique': [198, 208], 'based': [200, 210], 'on': [201, 211], 'streak': [203], 'camera': [204], 'system,': [205], 'time-correlated-single-photon-counting': [213], 'approach.': [214], 'Both': [215], 'allow': [217], 'biologists': [218], 'record': [220], 'emitted': [226], 'sample': [229], 'single': [232], 'measurement.': [233]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2066078187', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 3}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 6}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 9}, {'year': 2014, 'cited_by_count': 6}, {'year': 2013, 'cited_by_count': 3}, {'year': 2012, 'cited_by_count': 8}], 'updated_date': '2025-01-09T03:17:49.311381', 'created_date': '2016-06-24'}