Title: Identification and characterization of the glutamine-utilizing carbamyl phosphate synthetase activity in Drosophila melanogaster
Abstract: The predominant carbamyl phosphate synthetase activity in adult Drosophila melanogaster is a glutamine-utilizing, N-acetylglutamate-independent enzyme similar to that found in other eukaryotes. The synthetase poorly utilizes high levels of NH4+ in place of glutamine. Its activity is severely reduced in particular r mutants in agreement with earlier findings. The utilization of glutamine, Mg2+, and ATP by the enzyme are described. A pH optimum of 7.4–7.6 was determined. The enzyme's activity is inhibited by UTP, UDP, CTP, CDP, adenosine, AMP and free ATP and its activity is stimulated by 5-phosphoribosyl-1-pyrophosphate. These properties are discussed and compared to those of the enzyme from other organisms.
Publication Year: 1979
Publication Date: 1979-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 5
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