Abstract: Article1 May 2003free access CFTR directly mediates nucleotide-regulated glutathione flux Ilana Kogan Ilana Kogan Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Department of Physiology, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Mohabir Ramjeesingh Mohabir Ramjeesingh Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Canhui Li Canhui Li Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Jackie F. Kidd Jackie F. Kidd Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Yanchun Wang Yanchun Wang Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Elaine M. Leslie Elaine M. Leslie Cancer Research Laboratories, Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, K7L 3N6 Canada Search for more papers by this author Susan P.C. Cole Susan P.C. Cole Cancer Research Laboratories, Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, K7L 3N6 Canada Search for more papers by this author Christine E. Bear Corresponding Author Christine E. Bear Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Department of Physiology, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Ilana Kogan Ilana Kogan Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Department of Physiology, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Mohabir Ramjeesingh Mohabir Ramjeesingh Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Canhui Li Canhui Li Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Jackie F. Kidd Jackie F. Kidd Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Yanchun Wang Yanchun Wang Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Elaine M. Leslie Elaine M. Leslie Cancer Research Laboratories, Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, K7L 3N6 Canada Search for more papers by this author Susan P.C. Cole Susan P.C. Cole Cancer Research Laboratories, Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, K7L 3N6 Canada Search for more papers by this author Christine E. Bear Corresponding Author Christine E. Bear Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Department of Physiology, University of Toronto, Toronto, Ontario, M5G 1X8 Canada Search for more papers by this author Author Information Ilana Kogan1,2, Mohabir Ramjeesingh1, Canhui Li1, Jackie F. Kidd1, Yanchun Wang1, Elaine M. Leslie3, Susan P.C. Cole3 and Christine E. Bear 1,2 1Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8 Canada 2Department of Physiology, University of Toronto, Toronto, Ontario, M5G 1X8 Canada 3Cancer Research Laboratories, Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, K7L 3N6 Canada *Corresponding author. E-mail: [email protected] The EMBO Journal (2003)22:1981-1989https://doi.org/10.1093/emboj/cdg194 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Studies have shown that expression of cystic fibrosis transmembrane conductance regulator (CFTR) is associated with enhanced glutathione (GSH) efflux from airway epithelial cells, implicating a role for CFTR in the control of oxidative stress in the airways. To define the mechanism underlying CFTR-associated GSH flux, we studied wild-type and mutant CFTR proteins expressed in Sf9 membranes, as well as purified and reconstituted CFTR. We show that CFTR-expressing membrane vesicles mediate nucleotide-activated GSH flux, which is disrupted in the R347D pore mutant, and in the Walker A K464A and K1250A mutants. Further, we reveal that purified CFTR protein alone directly mediates nucleotide-dependent GSH flux. Interestingly, although ATP supports GSH flux through CFTR, this activity is enhanced in the presence of the non-hydrolyzable ATP analog AMP-PNP. These findings corroborate previous suggestions that CFTR pore properties can vary with the nature of the nucleotide interaction. In conclusion, our data demonstrate that GSH flux is an intrinsic function of CFTR and prompt future examination of the role of this function in airway biology in health and disease. Introduction Cystic fibrosis (CF) is a lethal autosomal recessive disease caused by mutations within the cystic fibrosis transmembrane conductance regulator (CFTR) gene (Boat et al., 1989). Most CF patients typically die of respiratory failure, which results from chronic inflammation and infection of the lung (Khan et al., 1995). Although mutations throughout the CFTR gene are known to cause disease, there is considerable debate regarding the primary defect(s) responsible for the pathogenesis of the CF respiratory phenotype. CFTR is a member of the ATP-binding cassette (ABC) superfamily, which includes other clinically important members, such as P-glycoprotein (Pgp) and the multidrug resistance protein, MRP1 (Riordan et al., 1985, 1989; Cole et al., 1992). All members of this family share a common domain organization, containing a minimum of two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs; Higgins, 1995). In contrast to Pgp and MRP1, which act as efflux pumps, CFTR protein is a phosphorylation- and nucleotide-regulated chloride channel (Anderson et al., 1991b; Tabcharani et al., 1991; Bear et al., 1992). Chloride channel activity by CFTR participates in the elaboration of salt and water transport at multiple sites in the respiratory epithelium. There is a general consensus that chloride conduction through CFTR has an essential role in driving fluid secretion by the submucosal glands underlying the surface epithelium of the airways (Boat et al., 1989; Frizzell, 1999). Conversely, on the apical membrane of the surface epithelium, CFTR is thought to provide the path for chloride absorption to accompany sodium absorption, probably through the ENaC channel (Boucher, 1999). The balance between these two transport functions is thought to lead to an optimal airway surface liquid (ASL) volume to promote ciliary clearance of mucus and bacteria. In CF, loss of functional CFTR expression is thought to disturb this balance between fluid secretion and absorption, leading to net volume depletion, increased viscosity and ineffective bacterial clearance (Boucher, 1999). Several reports have postulated that glutathione (GSH) deficiency also plays an essential role in the onset and progression of CF. High GSH concentrations, in the range of 400 μM, are found normally in ASL covering the respiratory epithelium (Cantin et al., 1987). However, decreased GSH levels have been detected in the ASL of patients with CF as well as in patients with other pulmonary diseases (Roum et al., 1993; Behr et al., 1995; Rahman and MacNee, 2000). Similarly, Gao et al. (1999) showed that GSH efflux from cells expressing mutant CFTR protein was reduced relative to that measured from cells transfected with wild-type CFTR. Moreover, in comparative in vivo studies of wild-type and CFTR knock-out mice, it was determined that GSH concentrations in the ASL obtained from mutant mice were significantly lower than those obtained from the normal mice (Velsor et al., 2001). Collectively, these data support the hypothesis that CFTR has an important role in the regulation of GSH flux from epithelial cells into the ASL. GSH is considered to be one of the body's most important intra- and extracellular antioxidants, providing protection against exposure to high levels of reactive oxygen species, to tissues such as the lung (Kelly, 1999). The redox status of GSH has also been implicated in the regulation of inflammation and the immune response, with GSH deficiency exacerbating inflammation and damage of the airways (Droge et al., 1991; Rahman and MacNee, 2000). In addition to GSH acting as an antioxidant, it also contributes to maintaining normal airway physiology by modifying mucus viscosity through the reduction of disulfide bonds in secreted mucins (Hudson, 2001). Moreover, recent results suggest that CFTR-dependent GSH flux could contribute to the differential sensitivity of epithelial cells to oxidative stress-induced apoptosis (Jungas et al., 2002). Therefore, in summary, there has been considerable speculation that mutations in CFTR may contribute, at least partially, to the low GSH levels observed in the ASL of CF patients and, subsequently, to the pathogenesis of CF respiratory disease. Linsdell and Hanrahan (1998b) were the first to demonstrate that CFTR expression was associated with GSH conductance. These authors showed that ‘macro’ membrane patches excised from cells transfected with CFTR could mediate GSH currents. Similar to the chloride channel function of CFTR, the GSH currents were activated by the addition of both cAMP-dependent kinase and MgATP (agents required for CFTR chloride channel function) and were blocked by chloride channel blockers, such as DNDS (dinitrostilbene-2,2′-disulfonate) and glibenclamide. However, the authors found that the GSH conductance was asymmetric, as inward GSH currents were minimal relative to outward currents. They had observed this phenomenon with a number of other large organic anions in their previous work (Linsdell and Hanrahan, 1998a). Further, as GSH blocked chloride currents through CFTR from the intracellular solution, the authors hypothesized that GSH directly accesses the CFTR channel pore to compete with chloride ion permeation. However, these studies did not establish that CFTR itself could mediate GSH flux directly. It is well known that other chloride channel activities can be regulated by phosphorylation and MgATP (e.g. ORCC and pseudo-CFTR channels; Schwiebert et al., 1994; Jovov et al., 1995; Marvao et al., 1998; Tsumura et al., 1998) and inhibited by DNDS (e.g. ORCC and Ca-activated channels; Venglarik et al., 1994; Fuller et al., 2001; Paradiso et al., 2001) or glibenclamide (Yamazaki and Hume, 1997; Gyomorey et al., 2000). Furthermore, significant data have been amassed over the years which suggest that CFTR can interact with and modify the activity of associated chloride transport proteins such as the HCO3−/Cl− antiporter (Lee et al., 1999; Ahn et al., 2001) and ClC-3 (Ogura et al., 2002). These observations raise the possibility that the GSH flux measured in CFTR-transfected cells may be mediated by a CFTR-regulated transporter. The primary goal of the present studies was to determine whether CFTR could mediate GSH flux directly. We tested this hypothesis by measuring nucleotide-regulated GSH flux into ‘inside-out’ plasma membrane vesicles prepared from Sf9 cells, expressing either wild-type or mutant CFTR proteins. Furthermore, to assess directly the intrinsic ability of CFTR to mediate GSH flux, we studied nucleotide-dependent GSH flux into proteoliposomes containing purified and functionally reconstituted CFTR. Results Expression of CFTR in Sf9 membranes confers a pathway that mediates GSH flux To assess the role of CFTR in GSH permeation, we compared GSH uptake by ‘inside-out’ membrane vesicles prepared from Sf9 cells expressing CFTR with GSH uptake by ‘inside-out’ membrane vesicles prepared from non-transfected cells. Uptake by ‘inside-out’ membrane vesicles can be equated to efflux from the cytosol to the extracellular solution. In both cases, the vesicles were pre-phosphorylated using the catalytic subunit of protein kinase A (PKA) in the presence of 1 mM ATP, to reproduce the conditions under which GSH conductance was detected originally in patch clamp studies (Linsdell and Hanrahan, 1998b). Uptake was initiated by the addition of [35S]GSH plus either MgATP (10 mM) or MgAMP-PNP (10 mM) in the presence of 1 mM cold GSH, a concentration previously shown not to have non-specific effects on CFTR function (Kogan et al., 2001). As shown in Figure 1A, no significant GSH flux was conferred by CFTR expression in the presence of MgATP. On the other hand, in the presence of MgAMP-PNP, there was an unequivocal increase in GSH flux mediated by CFTR-expressing Sf9 membranes, relative to Sf9 membranes not expressing CFTR (P = 0.0004). Therefore, the subsequent kinetic studies of CFTR-dependent GSH flux were conducted in the presence of MgAMP-PNP. Figure 1.Sf9 membrane vesicles expressing phosphorylated CFTR mediate nucleotide-dependent GSH flux. (A) PKA-phosphorylated membrane vesicles either expressing or not expressing wild-type CFTR protein were incubated with [35S]GSH and 1 mM cold GSH in the presence of either MgATP or MgAMP-PNP (n = 4–6). GSH flux in membrane vesicles containing equal amounts of CFTR was expressed as a percentage of GSH flux measured in membrane vesicles with no CFTR. The asterisk represents a statistically significant difference in CFTR-mediated GSH flux in vesicles treated with MgATP versus vesicles treated with MgAMP-PNP (Student's t-test; *P = 0.0004). (B) GSH uptake into vesicles expressing (filled squares) or not expressing CFTR (filled circles) was measured in the presence of MgAMP-PNP. GSH flux was normalized according to the amount of total protein in each treatment group. Curve fitting was performed by non-linear regression analysis, using the Michaelis–Menten equation. The data points represent the mean of duplicate values. (C) Kinetic parameters of CFTR-dependent GSH flux in the presence of MgAMP-PNP. A graph representing GSH flux by wild-type CFTR in the presence of MgAMP-PNP was obtained by subtracting GSH uptake values of vesicles with no CFTR from vesicles expressing CFTR (B). GSH flux values were expressed relative to the amount of CFTR in different preparations. Curve fitting was performed by non-linear regression analysis, using the Michaelis–Menten equation, to yield the following kinetic parameters: Km = 0.47 mM, Vmax = 612 pmol GSH/μg CFTR/h, r2 = 0.92. For all panels, CFTR quantitation is as described in Materials and methods. Download figure Download PowerPoint As shown in Figure 1B, membrane vesicles expressing CFTR exhibited concentration-dependent GSH flux, which saturated at increasing GSH concentrations. GSH uptake by membrane vesicles prepared from non-transfected cells was significantly less than that measured for vesicles expressing CFTR. In this figure, GSH flux in preparations with or without CFTR was normalized according to the amount of total protein in each treatment group. Subtraction of the uptake data measured in non-transfected vesicles from those measured in CFTR-transfected vesicles yielded GSH flux values that are mediated specifically by CFTR protein. These flux values, therefore, are expressed relative to the amount of CFTR protein, determined as described in Materials and methods. GSH flux by CFTR in the presence of MgAMP-PNP could be described by a Michaelis–Menten curve with a Km of 0.47 mM and a Vmax of 612 pmol GSH/μg CFTR/h (Figure 1C). The time dependence of GSH accumulation in Sf9 membrane vesicles expressing phosphorylated CFTR was determined in the presence of 10 mM MgAMP-PNP. Figure 2A shows an appreciable increase of GSH uptake by 5 min, reaching 0.7 pmol/μg total protein at 60 min. In contrast, membrane vesicles prepared from non-transfected Sf9 cells showed no appreciable [35S]GSH uptake over this time period, supporting the idea that this function is conferred specifically by CFTR. Figure 2.Characterization of CFTR-dependent GSH flux in the presence of MgAMP-PNP. (A) Time dependence of [35S]GSH uptake by phosphorylated membrane vesicles either expressing wild-type CFTR protein (filled circles) or not expressing CFTR (open circles). All uptake reactions contained 65 μg of total protein and were carried out for 1, 5, 10, 30 or 60 min. GSH flux was normalized according to the amount of total protein in each treatment group. Flux values are expressed as the mean ± SEM (n = 2–4). (B) The osmotic sensitivity of [35S]GSH uptake in the presence of MgAMP-PNP was determined by incubating CFTR-expressing vesicles in transport buffer containing various sucrose concentrations, ranging from zero to 750 mM. Values are expressed as the mean ± SEM (n = 2–4). Uptake values were normalized according to the amount of CFTR protein. Download figure Download PowerPoint To confirm that GSH uptake by CFTR-expressing vesicles indeed represents uptake into the internal compartment of the vesicles, rather than surface binding, we examined the effect of increasing osmotic sensitivity on GSH uptake. Increasing the osmolarity of the transport buffer will shrink the vesicles‘ lumen, thus causing decreased accumulation of ions (Loe et al., 1996). As shown in Figure 2B, increasing the sucrose concentrations in the transport buffer from 0 to 750 mM caused a significant decrease in GSH accumulation in CFTR-containing vesicles, from 558 to 313 pmol/μg CFTR/h, respectively. Therefore, the observed increase in GSH accumulation in CFTR-expressing vesicles represents uptake into the vesicles’ lumen. Mutant CFTR proteins expressed in Sf9 membranes exhibit defective GSH flux Arginine at position 347 is thought to contribute to the chloride channel pore of CFTR either by providing a binding site for chloride (Tabcharani et al., 1993) or by stabilizing the overall pore architecture (Cotten and Welsh, 1999). We postulated that if GSH can permeate CFTR directly, GSH uptake would be impaired in vesicles expressing this variant. As seen in Figure 3A (insert), the R347D variant was expressed well in Sf9 membranes. We compared [35S]GSH uptake by vesicles containing either phosphorylated wild-type or R347D CFTR proteins in the presence of 1 mM GSH, as this concentration is close to the Km(GSH) and is known not to have any non-specific effects on non-pore regions of CFTR. Specifically, our previous studies showed that the ATPase activity of purified CFTR is not affected by the addition of 1 mM GSH (Kogan et al., 2001). In the current study, we found that [35S]GSH uptake was significantly decreased in membrane vesicles expressing the R347D mutant protein, relative to those expressing wild-type CFTR, with rates of GSH flux of 207 and 498 pmol/μg CFTR/h, respectively (Figure 3A; P < 0.0001). This observation suggests that the AMP-PNP-dependent GSH flux in Sf9 membranes expressing CFTR is due predominantly to CFTR function. Furthermore, as shown in Figure 3B, a significant decrease in GSH uptake by vesicles expressing the mutant protein was also observed throughout a wide range of GSH concentrations. Kinetic analyses revealed that vesicles with the R347D mutation exhibited an ∼75% decrease in the Vmax of GSH uptake compared with vesicles with wild-type CFTR, corresponding to 176 and 612 pmol GSH/μg CFTR/h, respectively (Table I). These analyses also suggest that GSH interaction with the pore of the R347D mutant is ∼4 times stronger than that with the wild-type pore, with Km(GSH) values of 0.11 and 0.47 mM, respectively. Modified affinity of GSH for the mutant channel is consistent with previous reports, suggesting altered interaction of GSH with the R347D pore (Kogan et al., 2001). Figure 3.Comparison of GSH flux by wild-type CFTR versus CFTR R347D protein. (A) Membrane vesicles expressing phosphorylated wild-type or R347D CFTR proteins were incubated with 20 nM [35S]GSH and 1 mM cold GSH, in the presence of MgAMP-PNP. The asterisk represents statistically significant differences in [35S]GSH flux, relative to vesicles expressing wild-type CFTR (Student's t-test; *P < 0.0001). Values are expressed as the mean ± SEM (n = 5 for wild-type CFTR, n = 2 for R347D). Inset: expression of CFTR in membranes from Sf9 cells transfected with wild-type or R347D CFTR constructs. Membranes were solubilized in 2% SDS and Laemmli solubilizer and subjected to 4–12% SDS–PAGE. Immunoblotting was performed using the monoclonal anti-CFTR antibody M3A7 diluted 1:1000 (Chemicon International, Tamecula, CA). (B) Effect of increasing substrate concentration on [35S]GSH uptake by vesicles expressing phosphorylated wild-type CFTR or the R347D variant, in the presence of MgAMP-PNP. A graph representing GSH flux by wild-type CFTR is described in Figure 1C. [35S]GSH uptake by vesicles expressing the R347D protein was also obtained by subtracting GSH uptake values of vesicles with no CFTR from those of vesicles expressing the R347D variant. Curve fitting was performed by non-linear regression analysis, using the Michaelis–Menten equation, to yield the following kinetic parameters for the R347D mutant: Km = 0.11 mM, Vmax = 176 pmol GSH/μg CFTR/h, r2 = 0.97. GSH flux values were expressed relative to the amount of CFTR in different preparations. Download figure Download PowerPoint Table 1. Kinetic parameters of GSH uptake by Sf9 membrane vesicles containing PKA-phosphorylated wild-type or R347D CFTR proteins, in the presence of MgAMP-PNP Variables Wild type R347D Vmax (pmol/μg CFTR/h) 612 176 Km (mM) 0.47 0.11 To assess the nucleotide dependence of GSH permeation through CFTR, we determined the consequences of lysine mutations in the conserved Walker A consensus motifs for ATP binding in NBD1 and NBD2: K464A and K1250A, respectively. The function of these CFTR mutant proteins has been studied extensively, and it is well established that both mutations exert a disruptive effect on nucleotide interaction with CFTR (Ramjeesingh et al., 1999b; Aleksandrov et al., 2002). In Figure 4, we show that both the K464A and K1250A mutants exhibit similar significant reductions in GSH flux. We observed that GSH uptake in both the K464A and K1250A membrane vesicles was 3- to 4-fold lower than in vesicles expressing wild-type CFTR protein, yielding permeability values of 132 and 120 pmol/μg CFTR/h, respectively (P < 0.001). These findings support the notion that CFTR mediates nucleotide-regulated GSH flux. Figure 4.Relative contribution of the two NBDs to GSH flux by CFTR protein. Membrane vesicles expressing phosphorylated wild-type, K464A or K1250A CFTR were incubated with 20 nM [35S]GSH and 1 mM cold GSH in CFTR transport buffer, in the presence of MgAMP-PNP. [35S]GSH uptake by different preparations was analyzed by one-way ANOVA, followed by Bonferroni's multiple comparison test. The asterisks represent statistically significant differences in [35S]GSH uptake relative to vesicles expressing wild-type CFTR (*P < 0.001). Values shown represent the mean activity (± SEM; for K464A and K1250A, n = 4; for wild-type CFTR, n = 5). Inset: expression of CFTR in membranes from Sf9 cells transfected with wild-type, K464A or K1250A CFTR constructs. Western blot procedures are as described in Figure 3A. Download figure Download PowerPoint Purified and reconstituted wild-type CFTR protein supports GSH permeation into proteoliposomes To confirm that GSH permeability is indeed intrinsic to CFTR and to characterize further the nucleotide dependence of this property, GSH accumulation was measured in proteoliposomes containing purified and reconstituted wild-type CFTR protein. CFTR protein containing a polyhistidine tag was expressed in Sf9 cells, purified and reconstituted into pure phospholipid liposomes, as previously described (Ramjeesingh et al., 1999a; Kogan et al., 2002). Examination of CFTR-containing proteoliposomes by electron microscopy and negative staining revealed that most were spherical with a diameter of 30–60 nm, and a few with a larger diameter of ∼100 nm (Figure 5A). Radioactive GSH uptake experiments with purified and reconstituted CFTR protein were performed with PKA-phosphorylated protein to ensure that CFTR channels could gate to the open configuration (Ramjeesingh et al., 1999a). After the phosphorylation reaction, both PKA and MgATP were removed by dialysis. Figure 5.GSH flux by purified and reconstituted CFTR protein. (A) Electron microscopy of purified and reconstituted wild-type CFTR. Proteoliposomes on carbon formvar-coated grids were stained with 2% uranyl acetate and visualized by negative staining. Magnification is ×150 000. Bar = 100 nm. (B) Increasing concentrations of reconstituted and phosphorylated wild-type CFTR protein (0.6–6.5 μg) were incubated with 20 nM [35S]GSH and 1 mM cold GSH in CFTR transport buffer, in the presence of MgAMP-PNP. Each value represents the mean of duplicate determinations. Download figure Download PowerPoint As shown in Figure 5B, the degree of MgAMP-PNP-stimulated [35S]GSH flux mediated by proteoliposomes was dependent on the quantity of reconstituted CFTR protein. These data, therefore, indicate that CFTR can mediate GSH flux directly. We then compared the nucleotide-dependent regulation of [35S]GSH flux and 36Cl− flux in parallel studies of purified, reconstituted CFTR protein (Figure 6). In both assays, CFTR protein was pre-phosphorylated, as previously described, and the uptake initiated by addition of either [35S]GSH or 36Cl−. Uptake by proteoliposomes was assessed under three conditions: no added nucleotide, 1 mM MgATP or 6 mM MgAMP-PNP. For both anions, uptake was enhanced by the addition of nucleotide, confirming that both [35S]GSH flux and 36Cl− flux activities are nucleotide dependent. As predicted on the basis of substantial electrophysiological data (Vergani et al., 2003), 36Cl− flux was markedly stimulated by the addition of MgATP (Figure 6B; P < 0.001). On the other hand, no significant 36Cl− flux was mediated by proteoliposomes treated with MgAMP-PNP. On the contrary, the results obtained in our studies of [35S]GSH flux showed different nucleotide specificity. While MgATP stimulated [35S]GSH flux considerably, MgAMP-PNP caused a significantly greater response (Figure 6A; P < 0.05 and P < 0.001, respectively). The mean values for GSH permeation in the presence of MgATP or MgAMP-PNP are 3.5 and 9.8 pmol/μg CFTR/h, respectively. Collectively, our data support the concept that there may be differential permeation properties of CFTR that are mediated by interaction with MgATP or MgAMP-PNP. The 50-fold decrease in GSH flux observed in vesicles expressing purified and reconstituted wild-type CFTR, as compared with Sf9 membrane vesicles expressing CFTR, can be accounted for by the fact that only ∼50% of the reconstituted proteins display an ‘inside-out’ orientation. Further, it is estimated that only ∼10% of reconstituted CFTR in the proteoliposomes is truly active (Bear et al., 1992). Taking these considerations into account, the overall GSH flux in CFTR-expressing membrane vesicles is only 2- to 3-fold greater, as compared with that measured in CFTR proteoliposomes. Figure 6.Nucleotide-regulated GSH versus chloride flux by purified and reconstituted CFTR protein. (A) Proteoliposomes containing purified, reconstituted and phosphorylated CFTR were incubated with 20 nM [35S]GSH and 1 mM cold GSH at 33°C in CFTR transport buffer, in the presence of MgATP, MgAMP-PNP or no nucleotides for 60 min. GSH uptake values were normalized according to the amount of CFTR in each preparation. Values shown represent the mean activity (± SEM; n = 2–6). [35S]GSH uptake by different preparations was analyzed by one-way ANOVA, followed by Bonferroni's multiple comparison test. The asterisks represent statistically significant differences in [35S]GSH flux relative to vesicles treated with no nucleotides (*P < 0.05; **P < 0.001). (B) 36Cl− flux in purified, reconstituted and phosphorylated CFTR was measured at 33°C in the presence of MgATP, MgAMP-PNP or no nucleotides, as previously described (Li, 1996). Flux values were normalized according to the amount of CFTR in each preparation. Values shown represent the mean activity (± SEM; n = 3). Chloride flux by different preparations was analyzed by one-way ANOVA, followed by Bonferroni's multiple comparison test. The asterisks represent statistically significant differences in flux relative to vesicles treated with no nucleotides (*P < 0.001). Download figure Download PowerPoint Discussion Since the discovery of CFTR in 1989, there has been considerable debate regarding the spectrum of its biological function(s). Initially, the primary issue lay in establ