Title: High‐performance liquid chromatography with fluorescence detection of ebiratide using 4‐(<i>N, N</i>‐dimethylamino‐sulphonyl)‐7‐fluoro‐2, 1, 3‐benzoxadiazole as a fluorogenic reagent
Abstract:A sensitive high-performance liquid chromatography (HPLC) method with fluorescence detection has been developed and applied to the determination of ACTH4-9 analogue (ebiratide). 4-(N,N-Dimethylamino-s...A sensitive high-performance liquid chromatography (HPLC) method with fluorescence detection has been developed and applied to the determination of ACTH4-9 analogue (ebiratide). 4-(N,N-Dimethylamino-sulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F) was used as a fluorogenic labelling reagent. Ebiratide in 0.1 M borate buffer (pH 9.0) was reacted with DBD-F in acetonitrile solution at 50 degrees C for 30 min. After cooling, the mixture was separated by a reversed-phase HPLC. Then DBD-ebiratide was monitored at 580 nm with the excitation at 440 nm. The calibration curve was linear up to 10 pmol on column (r = 0.999) with the detection limit of 0.25 pmol (signal-to-noise = 3).Read More
Publication Year: 1995
Publication Date: 1995-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 4
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