Title: Development of 16S rRNA Oligonucleotide Probes for Brevibacterium, Micrococcus/Arthrobacter and Microbacterium/Aureobacterium Used in Dairy Starter Cultures
Abstract: Oligonucleotide probes for bacteria of the cheese surface were developed. The complete 16S rRNA nucleotide sequences available from databanks of representatives of the genera Brevibacterium, Microbacterium/Aureobacterium and Micrococcus/Arthrobacter were aligned. Oligonucleotide probes were defined for the identification of these genera by selecting a common sequence of their 16S rRNA genes which are different from related genera like Corynebacterium, Propionibacterium or Bifidobacterium. 82 strains were tested of which 70 were of the high DNA G+C branch of bacterial evolution. It was shown by colony-hybridization experiments, that the selected oligonucleotide probes targeted to the 16S rDNA region hybridized only with nucleic acids from strains of Brevibacterium, Microbacterium/Aureobacterium and Micrococcus/Arthrobacter, respectively. For confirmation, a part of the 16S rDNA was amplified in vitro using the polymerase chain reaction technique with two specific oligonucleotides for each of these bacterial groups. The tested probes provide a highly efficient tool to investigate and identify these genera within the coryneform group of bacteria. They were especially developed for analyzing the composition of the microflora of the cheese surface. The use of them may contribute substantially to the isolation and study of new strains of the these genera from natural habitats like cheese.
Publication Year: 1997
Publication Date: 1997-08-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 18
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