Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2063034608', 'doi': 'https://doi.org/10.1371/journal.pone.0033269', 'title': 'One-Step Purification of Recombinant Human Amelogenin and Use of Amelogenin as a Fusion Partner', 'display_name': 'One-Step Purification of Recombinant Human Amelogenin and Use of Amelogenin as a Fusion Partner', 'publication_year': 2012, 'publication_date': '2012-03-19', 'ids': {'openalex': 'https://openalex.org/W2063034608', 'doi': 'https://doi.org/10.1371/journal.pone.0033269', 'mag': '2063034608', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/22442680', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/3307724'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0033269', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033269&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'doaj', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033269&type=printable', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5061319042', 'display_name': 'Johan Bonde', 'orcid': 'https://orcid.org/0000-0001-8866-7982'}, 'institutions': [{'id': 'https://openalex.org/I187531555', 'display_name': 'Lund University', 'ror': 'https://ror.org/012a77v79', 'country_code': 'SE', 'type': 'education', 'lineage': ['https://openalex.org/I187531555']}], 'countries': ['SE'], 'is_corresponding': False, 'raw_author_name': 'Johan Svensson Bonde', 'raw_affiliation_strings': ['Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden'], 'affiliations': [{'raw_affiliation_string': 'Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden', 'institution_ids': ['https://openalex.org/I187531555']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5039580254', 'display_name': 'Leif Bülow', 'orcid': 'https://orcid.org/0000-0003-4966-8610'}, 'institutions': [{'id': 'https://openalex.org/I187531555', 'display_name': 'Lund University', 'ror': 'https://ror.org/012a77v79', 'country_code': 'SE', 'type': 'education', 'lineage': ['https://openalex.org/I187531555']}], 'countries': ['SE'], 'is_corresponding': False, 'raw_author_name': 'Leif Bulow', 'raw_affiliation_strings': ['Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden'], 'affiliations': [{'raw_affiliation_string': 'Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden', 'institution_ids': ['https://openalex.org/I187531555']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 1805, 'currency': 'USD', 'value_usd': 1805, 'provenance': 'doaj'}, 'apc_paid': {'value': 1805, 'currency': 'USD', 'value_usd': 1805, 'provenance': 'doaj'}, 'fwci': 2.081, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 32, 'citation_normalized_percentile': {'value': 0.942773, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 93, 'max': 94}, 'biblio': {'volume': '7', 'issue': '3', 'first_page': 'e33269', 'last_page': 'e33269'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11570', 'display_name': 'Periodontal Regeneration and Treatments', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2748', 'display_name': 'Urology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11570', 'display_name': 'Periodontal Regeneration and Treatments', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/2748', 'display_name': 'Urology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T12110', 'display_name': 'Bone and Dental Protein Studies', 'score': 0.999, 'subfield': {'id': 'https://openalex.org/subfields/2745', 'display_name': 'Rheumatology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T11919', 'display_name': 'dental development and anomalies', 'score': 0.9919, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/amelogenin', 'display_name': 'Amelogenin', 'score': 0.9982566}], 'concepts': [{'id': 'https://openalex.org/C106434640', 'wikidata': 'https://www.wikidata.org/wiki/Q2844797', 'display_name': 'Amelogenin', 'level': 3, 'score': 0.9982566}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.53739727}, {'id': 'https://openalex.org/C156887251', 'wikidata': 'https://www.wikidata.org/wiki/Q2386424', 'display_name': 'Enamel paint', 'level': 2, 'score': 0.48881266}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.37281024}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.24519336}, {'id': 'https://openalex.org/C199343813', 'wikidata': 'https://www.wikidata.org/wiki/Q12128', 'display_name': 'Dentistry', 'level': 1, 'score': 0.18619344}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.13359019}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.1295807}], 'mesh': [{'descriptor_ui': 'D053523', 'descriptor_name': 'Amelogenin', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D053523', 'descriptor_name': 'Amelogenin', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D053523', 'descriptor_name': 'Amelogenin', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D053523', 'descriptor_name': 'Amelogenin', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D015870', 'descriptor_name': 'Gene Expression', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010281', 'descriptor_name': 'Parathyroid Hormone', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D010281', 'descriptor_name': 'Parathyroid Hormone', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D010281', 'descriptor_name': 'Parathyroid Hormone', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D010281', 'descriptor_name': 'Parathyroid Hormone', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}], 'locations_count': 5, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0033269', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033269&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://doaj.org/article/b76465affd364274af36d79ba0a556a4', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306401280', 'display_name': 'DOAJ (DOAJ: Directory of Open Access Journals)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc3307724', 'pdf_url': 'https://europepmc.org/articles/pmc3307724?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307724', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/22442680', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0033269', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033269&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'score': 0.5, 'id': 'https://metadata.un.org/sdg/3', 'display_name': 'Good health and well-being'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 38, 'referenced_works': ['https://openalex.org/W1968527371', 'https://openalex.org/W1969203337', 'https://openalex.org/W1971737620', 'https://openalex.org/W1985877288', 'https://openalex.org/W1989634977', 'https://openalex.org/W1994562481', 'https://openalex.org/W1995774206', 'https://openalex.org/W2008428303', 'https://openalex.org/W2010762788', 'https://openalex.org/W2013827325', 'https://openalex.org/W2019916074', 'https://openalex.org/W2020667613', 'https://openalex.org/W2025342255', 'https://openalex.org/W2026863358', 'https://openalex.org/W2036931844', 'https://openalex.org/W2037152535', 'https://openalex.org/W2052338568', 'https://openalex.org/W2053399901', 'https://openalex.org/W2054778734', 'https://openalex.org/W2059125062', 'https://openalex.org/W2062745266', 'https://openalex.org/W2063237398', 'https://openalex.org/W2068521700', 'https://openalex.org/W2073859964', 'https://openalex.org/W2076064293', 'https://openalex.org/W2082584989', 'https://openalex.org/W2094184848', 'https://openalex.org/W2104748476', 'https://openalex.org/W2105780087', 'https://openalex.org/W2115440372', 'https://openalex.org/W2123300175', 'https://openalex.org/W2130888131', 'https://openalex.org/W2132620042', 'https://openalex.org/W2133902722', 'https://openalex.org/W2139778924', 'https://openalex.org/W2154629889', 'https://openalex.org/W2170610568', 'https://openalex.org/W2297594805'], 'related_works': ['https://openalex.org/W4311855708', 'https://openalex.org/W4214695268', 'https://openalex.org/W3172617238', 'https://openalex.org/W2168936688', 'https://openalex.org/W2156988415', 'https://openalex.org/W2098184301', 'https://openalex.org/W2017071525', 'https://openalex.org/W1965799294', 'https://openalex.org/W1926997387', 'https://openalex.org/W1016136338'], 'abstract_inverted_index': {'Amelogenin': [0], 'is': [1], 'an': [2, 119], 'extracellular': [3], 'protein': [4, 77, 130], 'first': [5], 'identified': [6], 'as': [7, 35, 91, 97], 'a': [8, 48, 80, 98, 124, 152], 'matrix': [9], 'component': [10], 'important': [11, 32], 'for': [12, 51], 'formation': [13], 'of': [14, 37, 53, 66, 75], 'dental': [15], 'enamel': [16], 'during': [17], 'tooth': [18], 'development.': [19], 'Lately,': [20], 'amelogenin': [21, 56, 83, 96, 135, 143], 'has': [22], 'also': [23, 109], 'been': [24], 'found': [25], 'to': [26, 113], 'have': [27], 'positive': [28], 'effects': [29], 'on': [30, 62], 'clinical': [31], 'areas,': [33], 'such': [34], 'treatment': [36], 'periodontal': [38], 'defects,': [39], 'wound': [40], 'healing,': [41], 'and': [42, 78, 134, 139, 141], 'bone': [43], 'regeneration.': [44], 'Here': [45], 'we': [46, 101], 'present': [47], 'simple': [49], 'method': [50, 69, 108], 'purification': [52], 'recombinant': [54], 'human': [55], 'expressed': [57, 138], 'in': [58, 84, 118], 'Escherichia': [59], 'coli,': [60], 'based': [61], 'the': [63, 76, 106, 127, 142], 'solubility': [64], 'properties': [65], 'amelogenin.': [67], 'The': [68], 'combines': [70], 'cell': [71], 'lysis': [72], 'with': [73], 'recovery/purification': [74], 'generates': [79], '>95%': [81], 'pure': [82], 'one': [85], 'step': [86], 'using': [87, 95, 151], 'intact': [88], 'harvested': [89], 'cells': [90], 'starting': [92], 'material.': [93], 'By': [94], 'fusion': [99, 125], 'partner': [100], 'could': [102, 145], 'further': [103], 'demonstrate': [104], 'that': [105], 'same': [107], 'be': [110, 146], 'can': [111], 'explored': [112], 'purify': [114], 'other': [115], 'target': [116], 'proteins/peptides': [117], 'effective': [120], 'manner.': [121], 'For': [122], 'instance,': [123], 'between': [126], 'clinically': [128], 'used': [129], 'PTH': [131, 149], '(parathyroid': [132], 'hormone)': [133], 'was': [136], 'successfully': [137], 'purified,': [140], 'part': [144], 'removed': [147], 'from': [148], 'by': [150], 'site-specific': [153], 'protease.': [154]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2063034608', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2023, 'cited_by_count': 2}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 3}, {'year': 2014, 'cited_by_count': 3}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-14T07:37:50.139566', 'created_date': '2016-06-24'}