Specificity and Enzyme Kinetics of the Quorum-quenching N-Acyl Homoserine Lactone Lactonase (AHL-lactonase)

Name: Work Video:
Duration: 3 min 30 s
Lianhui Wang; Lixing Weng; Yi‐Hu Dong; Lian‐Hui Zhang
{'id': 'https://openalex.org/W2062594481', 'doi': 'https://doi.org/10.1074/jbc.m311194200', 'title': 'Specificity and Enzyme Kinetics of the Quorum-quenching N-Acyl Homoserine Lactone Lactonase (AHL-lactonase)', 'display_name': 'Specificity and Enzyme Kinetics of the Quorum-quenching N-Acyl Homoserine Lactone Lactonase (AHL-lactonase)', 'publication_year': 2004, 'publication_date': '2004-03-26', 'ids': {'openalex': 'https://openalex.org/W2062594481', 'doi': 'https://doi.org/10.1074/jbc.m311194200', 'mag': '2062594481', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/14734559'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m311194200', 'pdf_url': 'http://www.jbc.org/article/S0021925819640263/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S0021925819640263/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5018432603', 'display_name': 'Lianhui Wang', 'orcid': 'https://orcid.org/0000-0001-9030-9172'}, 'institutions': [{'id': 'https://openalex.org/I75573869', 'display_name': 'Institute of Molecular and Cell Biology', 'ror': 'https://ror.org/04xpsrn94', 'country_code': 'SG', 'type': 'facility', 'lineage': ['https://openalex.org/I115228651', 'https://openalex.org/I2801752549', 'https://openalex.org/I75573869']}], 'countries': ['SG'], 'is_corresponding': False, 'raw_author_name': 'Lian-Hui Wang', 'raw_affiliation_strings': ['Institute of Molecular and Cell Biology 30 Medical Drive Singapore 117609'], 'affiliations': [{'raw_affiliation_string': 'Institute of Molecular and Cell Biology 30 Medical Drive Singapore 117609', 'institution_ids': ['https://openalex.org/I75573869']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5054027559', 'display_name': 'Lixing Weng', 'orcid': 'https://orcid.org/0000-0002-7719-892X'}, 'institutions': [{'id': 'https://openalex.org/I75573869', 'display_name': 'Institute of Molecular and Cell Biology', 'ror': 'https://ror.org/04xpsrn94', 'country_code': 'SG', 'type': 'facility', 'lineage': ['https://openalex.org/I115228651', 'https://openalex.org/I2801752549', 'https://openalex.org/I75573869']}], 'countries': ['SG'], 'is_corresponding': False, 'raw_author_name': 'Li-Xing Weng', 'raw_affiliation_strings': ['Institute of Molecular and Cell Biology 30 Medical Drive Singapore 117609'], 'affiliations': [{'raw_affiliation_string': 'Institute of Molecular and Cell Biology 30 Medical Drive Singapore 117609', 'institution_ids': ['https://openalex.org/I75573869']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5103962530', 'display_name': 'Yi‐Hu Dong', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I75573869', 'display_name': 'Institute of Molecular and Cell Biology', 'ror': 'https://ror.org/04xpsrn94', 'country_code': 'SG', 'type': 'facility', 'lineage': ['https://openalex.org/I115228651', 'https://openalex.org/I2801752549', 'https://openalex.org/I75573869']}], 'countries': ['SG'], 'is_corresponding': False, 'raw_author_name': 'Yi-Hu Dong', 'raw_affiliation_strings': ['Institute of Molecular and Cell Biology 30 Medical Drive Singapore 117609'], 'affiliations': [{'raw_affiliation_string': 'Institute of Molecular and Cell Biology 30 Medical Drive Singapore 117609', 'institution_ids': ['https://openalex.org/I75573869']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5074121127', 'display_name': 'Lian‐Hui Zhang', 'orcid': 'https://orcid.org/0000-0002-4298-7988'}, 'institutions': [{'id': 'https://openalex.org/I165932596', 'display_name': 'National University of Singapore', 'ror': 'https://ror.org/01tgyzw49', 'country_code': 'SG', 'type': 'education', 'lineage': ['https://openalex.org/I165932596']}, {'id': 'https://openalex.org/I75573869', 'display_name': 'Institute of Molecular and Cell Biology', 'ror': 'https://ror.org/04xpsrn94', 'country_code': 'SG', 'type': 'facility', 'lineage': ['https://openalex.org/I115228651', 'https://openalex.org/I2801752549', 'https://openalex.org/I75573869']}], 'countries': ['SG'], 'is_corresponding': False, 'raw_author_name': 'Lian-Hui Zhang', 'raw_affiliation_strings': ['Department of Biological Sciences, The National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.', 'Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609'], 'affiliations': [{'raw_affiliation_string': 'Department of Biological Sciences, The National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.', 'institution_ids': ['https://openalex.org/I165932596']}, {'raw_affiliation_string': 'Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609', 'institution_ids': ['https://openalex.org/I75573869']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 3.921, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 216, 'citation_normalized_percentile': {'value': 0.999979, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '279', 'issue': '14', 'first_page': '13645', 'last_page': '13651'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10593', 'display_name': 'Bacterial biofilms and quorum sensing', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10593', 'display_name': 'Bacterial biofilms and quorum sensing', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10195', 'display_name': 'Antimicrobial Resistance in Staphylococcus', 'score': 0.992, 'subfield': {'id': 'https://openalex.org/subfields/2725', 'display_name': 'Infectious Diseases'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10147', 'display_name': 'Antibiotic Resistance in Bacteria', 'score': 0.9894, 'subfield': {'id': 'https://openalex.org/subfields/1313', 'display_name': 'Molecular Medicine'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/homoserine', 'display_name': 'Homoserine', 'score': 0.629441}, {'id': 'https://openalex.org/keywords/quorum-quenching', 'display_name': 'Quorum Quenching', 'score': 0.5679125}, {'id': 'https://openalex.org/keywords/enzyme-kinetics', 'display_name': 'Enzyme Kinetics', 'score': 0.5006385}], 'concepts': [{'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.7122019}, {'id': 'https://openalex.org/C2779840497', 'wikidata': 'https://www.wikidata.org/wiki/Q418214', 'display_name': 'Homoserine', 'level': 5, 'score': 0.629441}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.62243086}, {'id': 'https://openalex.org/C118687296', 'wikidata': 'https://www.wikidata.org/wiki/Q905754', 'display_name': 'Quorum sensing', 'level': 4, 'score': 0.6039008}, {'id': 'https://openalex.org/C2908737351', 'wikidata': 'https://www.wikidata.org/wiki/Q905754', 'display_name': 'Quorum Quenching', 'level': 5, 'score': 0.5679125}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.56617475}, {'id': 'https://openalex.org/C56856141', 'wikidata': 'https://www.wikidata.org/wiki/Q883112', 'display_name': 'Enzyme kinetics', 'level': 4, 'score': 0.5006385}, {'id': 'https://openalex.org/C71240020', 'wikidata': 'https://www.wikidata.org/wiki/Q186011', 'display_name': 'Stereochemistry', 'level': 1, 'score': 0.43790004}, {'id': 'https://openalex.org/C2776414213', 'wikidata': 'https://www.wikidata.org/wiki/Q183290', 'display_name': 'Serine', 'level': 3, 'score': 0.41163948}, {'id': 'https://openalex.org/C41183919', 'wikidata': 'https://www.wikidata.org/wiki/Q423026', 'display_name': 'Active site', 'level': 3, 'score': 0.3346678}, {'id': 'https://openalex.org/C60987743', 'wikidata': 'https://www.wikidata.org/wiki/Q1460232', 'display_name': 'Virulence', 'level': 3, 'score': 0.19568366}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m311194200', 'pdf_url': 'http://www.jbc.org/article/S0021925819640263/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m311194200', 'pdf_url': 'http://www.jbc.org/article/S0021925819640263/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'display_name': 'Clean water and sanitation', 'score': 0.56, 'id': 'https://metadata.un.org/sdg/6'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 42, 'referenced_works': ['https://openalex.org/W110302458', 'https://openalex.org/W1524366924', 'https://openalex.org/W1590944458', 'https://openalex.org/W1593567129', 'https://openalex.org/W1703657909', 'https://openalex.org/W1754186508', 'https://openalex.org/W191752848', 'https://openalex.org/W1967221794', 'https://openalex.org/W1970582294', 'https://openalex.org/W1974257886', 'https://openalex.org/W1977105094', 'https://openalex.org/W1983108555', 'https://openalex.org/W1983837274', 'https://openalex.org/W198863919', 'https://openalex.org/W1997418937', 'https://openalex.org/W2008119892', 'https://openalex.org/W2015509909', 'https://openalex.org/W2019514014', 'https://openalex.org/W2026847863', 'https://openalex.org/W2036553709', 'https://openalex.org/W2042544884', 'https://openalex.org/W2044385230', 'https://openalex.org/W2046383099', 'https://openalex.org/W2062438057', 'https://openalex.org/W2079405427', 'https://openalex.org/W2084431722', 'https://openalex.org/W2089938357', 'https://openalex.org/W2091710634', 'https://openalex.org/W2092550686', 'https://openalex.org/W2107304641', 'https://openalex.org/W2113490404', 'https://openalex.org/W2125268958', 'https://openalex.org/W2126922170', 'https://openalex.org/W2127799152', 'https://openalex.org/W2138776000', 'https://openalex.org/W2141016491', 'https://openalex.org/W2141425531', 'https://openalex.org/W2144150209', 'https://openalex.org/W2150533377', 'https://openalex.org/W2161375631', 'https://openalex.org/W2169772157', 'https://openalex.org/W4229932570'], 'related_works': ['https://openalex.org/W785691620', 'https://openalex.org/W4389794015', 'https://openalex.org/W4386950863', 'https://openalex.org/W3006679416', 'https://openalex.org/W2791554914', 'https://openalex.org/W2488488572', 'https://openalex.org/W2170364456', 'https://openalex.org/W2091710634', 'https://openalex.org/W2016957700', 'https://openalex.org/W1529266958'], 'abstract_inverted_index': {'N-Acyl': [0, 253], 'homoserine': [1, 254, 565, 591, 688, 727, 1830, 3937], 'lactone': [2, 37, 255, 290, 728, 1116, 1126, 1812, 3051], '(AHL)': [3, 256], 'quorum-sensing': [4, 12, 257, 265, 555, 1447], 'signals': [5, 258, 656, 681, 696, 1498, 3946], 'are': [6, 259, 1690, 3287, 3553, 4352, 4358, 4364], 'the': [7, 36, 46, 48, 92, 95, 99, 107, 111, 114, 118, 141, 166, 194, 199, 232, 260, 289, 299, 301, 345, 348, 352, 360, 364, 367, 371, 394, 419, 447, 452, 485, 525, 663, 715, 720, 725, 776, 824, 833, 843, 1014, 1057, 1110, 1125, 1129, 1429, 1446, 1501, 1540, 1598, 1615, 1642, 1693, 1714, 1730, 1733, 1738, 1777, 1827, 1920, 1968, 1974, 1977, 2025, 2037, 2048, 2115, 2130, 2139, 2146, 2216, 2231, 2244, 2289, 2300, 2348, 2369, 2374, 2396, 2401, 2422, 2441, 2474, 2516, 2526, 2556, 2604, 2619, 2630, 2642, 2746, 2768, 2773, 2788, 2798, 2803, 2829, 2841, 2845, 2892, 2897, 2919, 2927, 2930, 2969, 2972, 2983, 3032, 3058, 3067, 3072, 3090, 3118, 3135, 3180, 3190, 3205, 3210, 3221, 3225, 3266, 3317, 3353, 3407, 3443, 3452, 3456, 3470, 3475, 3480, 3495, 3513, 3556, 3579, 3654, 3662, 3680, 3687, 3695, 3720, 3724, 3766, 3780, 3790, 3799, 3812, 3817, 3900, 3934, 3969, 3988, 4014, 4024, 4036, 4064, 4097, 4106, 4120, 4147, 4217, 4255, 4258, 4297, 4313, 4321, 4325, 4334, 4377], 'vital': [8, 261], 'elements': [9, 262], 'of': [10, 91, 98, 117, 121, 144, 147, 193, 218, 234, 263, 344, 351, 370, 374, 397, 400, 446, 471, 487, 527, 539, 652, 679, 694, 719, 778, 781, 835, 850, 1056, 1107, 1302, 1448, 1453, 1496, 1543, 1696, 1717, 1722, 1737, 1742, 1787, 1835, 1901, 1976, 2027, 2033, 2040, 2208, 2312, 2351, 2357, 2361, 2399, 2432, 2485, 2539, 2581, 2632, 2636, 2662, 2674, 2691, 2706, 2745, 2802, 2831, 2838, 2856, 2916, 2918, 2929, 2955, 2971, 2987, 3048, 3057, 3161, 3177, 3224, 3233, 3249, 3349, 3352, 3366, 3388, 3400, 3442, 3461, 3469, 3497, 3516, 3522, 3561, 3583, 3640, 3650, 3683, 3698, 3723, 3740, 3751, 3761, 3770, 3820, 3961, 3972, 3982, 3997, 4053, 4057, 4063, 4070, 4105, 4162, 4216, 4235, 4271, 4376], 'bacterial': [11, 264, 646, 706, 856, 1031, 1049, 1138, 1440, 1449, 2041], 'systems,': [13, 266], 'which': [14, 171, 241, 267, 424, 494, 542, 712, 1119, 1536, 1610, 2740, 2881, 2947, 2979, 3061, 3081, 3483, 3741, 4177, 4204], 'regulate': [15, 268], 'diverse': [16, 269, 544, 836], 'biological': [17, 270, 545, 839, 3888], 'functions,': [18, 271, 840], 'including': [19, 272, 547, 3298, 4160, 4221], 'virulence.': [20, 273], 'The': [21, 102, 274, 355, 655, 815, 1528, 1819, 1847, 1955, 1990, 2015, 2057, 2096, 2108, 2153, 2167, 2314, 2359, 2430, 2467, 2518, 2574, 2610, 2634, 2694, 2714, 2751, 2762, 2836, 2877, 2907, 2922, 2965, 3009, 3045, 3074, 3159, 3227, 3245, 3376, 3397, 3439, 3518, 3551, 3642, 3786, 3805, 3876, 3993, 4050, 4077, 4246], 'AHL-lactonase,': [22, 275, 2400, 3664], 'a': [23, 134, 226, 237, 276, 387, 479, 490, 649, 659, 692, 848, 1003, 1022, 1434, 1486, 1531, 1720, 2030, 2074, 2101, 2309, 2490, 2548, 2590, 2671, 2688, 2914, 3175, 3386, 3509, 3568, 3575, 3609, 3637, 3659, 3746, 3883, 3953, 3983, 4047, 4068, 4084, 4127, 4134, 4179, 4206, 4269], 'quorumquenching': [24, 277], 'enzyme': [25, 80, 162, 219, 229, 278, 333, 415, 472, 482, 1529, 1706, 1739, 1745, 1757, 2168, 2402, 2442, 2469, 2665, 2763, 2776, 2810, 2909, 2975, 3075, 3136, 3164, 3181, 3200, 3307, 3354, 3395, 3444, 3487, 3558, 3696, 3762, 3781, 4065, 4078, 4121, 4298, 4326], 'encoded': [26, 279, 3405], 'by': [27, 34, 280, 287, 1052, 1122, 1825, 1882, 1926, 1960, 1994, 2024, 2060, 2086, 2127, 2150, 2172, 2236, 2304, 2319, 2368, 2446, 2515, 2608, 2702, 2723, 2767, 2850, 2871, 2904, 3055, 3084, 3265, 3324, 3338, 3406, 3627, 3666, 3676, 3692, 3755, 3783, 3879, 4061, 4240, 4253], 'aiiA': [28, 281, 1906, 3408], 'from': [29, 282, 1136, 1815, 1895, 2125, 2388, 2578, 2902, 3027, 3220, 3529, 4017, 4039, 4354, 4360, 4366], 'Bacillus': [30, 283], 'sp.,': [31, 284], 'inactivates': [32, 285], 'AHLs': [33, 122, 286, 375, 1504, 1788, 1865, 3711, 4141, 4274, 4314, 4335], 'hydrolyzing': [35, 288], 'bond': [38, 291, 1127], 'to': [39, 71, 140, 175, 292, 324, 393, 428, 513, 517, 532, 658, 673, 1029, 1046, 1405, 1438, 1444, 1484, 1539, 1638, 1775, 1851, 1967, 2029, 2100, 2114, 2165, 2308, 2391, 2412, 2524, 2677, 2711, 2817, 3029, 3103, 3174, 3216, 3237, 3256, 3347, 3493, 3531, 3578, 3734, 3763, 3882, 3894, 3903, 3942, 3979, 4157, 4189, 4257, 4373], 'produce': [40, 293, 709], 'corresponding': [41, 294, 1828, 2181, 3062, 3800, 4025, 4343], 'N-acyl': [42, 295, 687, 3936], 'homoserines.': [43, 296], 'To': [44, 297, 1728, 2394, 3604], 'characterize': [45, 298], 'enzyme,': [47, 300, 3120], 'recombinant': [49, 302, 2878, 2908, 2932], 'AHL-lactonase': [50, 64, 150, 224, 235, 303, 317, 403, 477, 488, 1296, 1395, 1482, 1643, 1718, 1753, 1902, 1909, 2119, 2142, 2155, 2196, 2266, 2273, 2343, 2364, 2448, 2486, 2540, 2667, 2933, 2974, 3013, 3021, 3107, 3320, 3401, 3404, 3462, 3500, 3523, 3562, 3565, 3573, 3626, 3699, 3729, 3756, 3880, 4058, 4098, 4125, 4137, 4152, 4171, 4211, 4263], 'and': [51, 58, 74, 89, 110, 183, 203, 215, 230, 304, 311, 327, 342, 363, 436, 456, 468, 483, 511, 523, 549, 666, 717, 837, 854, 862, 1114, 1128, 1292, 1305, 1400, 1499, 1508, 1606, 1705, 1725, 1740, 1765, 1783, 1789, 1858, 1866, 1875, 1879, 1886, 1903, 1910, 1916, 1932, 1962, 1964, 2019, 2062, 2118, 2120, 2145, 2156, 2192, 2215, 2238, 2274, 2299, 2420, 2436, 2507, 2541, 2561, 2601, 2660, 2709, 2717, 2731, 2753, 2775, 2783, 2787, 2811, 2822, 2844, 2854, 2869, 2889, 3117, 3124, 3230, 3241, 3276, 3285, 3290, 3301, 3311, 3329, 3335, 3340, 3357, 3383, 3474, 3533, 3545, 3620, 3672, 3674, 3705, 3717, 3772, 3795, 3890, 3968, 4074, 4079, 4088, 4114, 4165, 4228, 4248, 4277, 4283, 4290, 4307, 4330], 'its': [52, 305, 1702, 1911, 2020, 2121, 2157, 2542, 2718, 3143, 3415], 'four': [53, 306, 1502, 1752], 'variants': [54, 307, 1754, 1912, 2021, 2122, 2158, 2543], 'were': [55, 308, 1044, 1134, 1791, 1823, 1868, 1893, 1923, 1958, 1992, 2022, 2070, 2112, 2123, 2143, 2159, 2283, 2293, 2377, 2444, 2487, 2512, 2522, 2576, 2696, 2721, 2727, 2734, 2756, 2778, 2815, 2847, 3235, 3263, 3966, 3995, 4186, 4251], 'purified.': [56, 309], 'Kinetic': [57, 310, 4233], 'substrate': [59, 312, 1703, 2275, 2747, 2774, 2799, 3764, 4055, 4080, 4244, 4378], 'specificity': [60, 313, 780, 1704, 2764, 4056], 'analysis': [61, 185, 314, 438, 1767, 2323, 2924, 2967, 3056, 3086], 'showed': [62, 190, 315, 443, 1299, 1401, 2968, 3303, 3730, 3789, 4153, 4172, 4264, 4299], 'that': [63, 106, 191, 223, 231, 316, 359, 444, 476, 484, 1006, 1442, 1809, 2373, 2772, 2926, 3451, 3506, 3572, 3656, 3816, 4008, 4030, 4096, 4124], 'had': [65, 318, 2748, 3391, 3807], 'no': [66, 319, 2741, 3093, 3304, 3392, 3630, 4009, 4031], 'or': [67, 154, 157, 247, 320, 407, 410, 500, 1297, 1687, 2321, 3094, 3105, 3826], 'little': [68, 321, 2951, 3095, 4173], 'residue': [69, 169, 322, 422], 'activity': [70, 81, 323, 334, 1644, 1716, 1746, 1758, 2344, 2365, 2424, 2443, 2470, 3014, 3096, 3144, 3166, 3182, 3308, 3445, 3559, 3685, 3697, 3897, 3960, 4010, 4020, 4032, 4066, 4156, 4174, 4267], 'non-acyl': [72, 325, 4072, 4158], 'lactones': [73, 326, 689, 1831, 4161, 4167], 'noncyclic': [75, 328], 'esters,': [76, 329, 4220], 'but': [77, 330, 723, 3539], 'displayed': [78, 331, 3183, 4327], 'strong': [79, 332], 'toward': [82, 335, 3700, 4103, 4131, 4175, 4312], 'all': [83, 336, 2732, 3932, 4104], 'tested': [84, 337, 1503, 3706, 4107, 4218], 'AHLs,': [85, 338, 711, 3702, 3821, 4071], 'varying': [86, 339], 'in': [87, 128, 198, 340, 381, 451, 530, 534, 698, 702, 714, 832, 841, 847, 1011, 1039, 1614, 1756, 1772, 1832, 1919, 1983, 2007, 2064, 2205, 2276, 2285, 2382, 2404, 2457, 2555, 2629, 2729, 2840, 2863, 3060, 3066, 3089, 3189, 3193, 3447, 3467, 3526, 3625, 3713, 3757, 3765, 3811, 3872, 3887, 3947, 4013, 4035, 4046, 4083, 4110, 4379], 'length': [88, 341, 716, 2571, 3716, 4113], 'nature': [90, 343], 'substitution': [93, 192, 346, 445, 718, 3718, 4115, 4144, 4347], 'at': [94, 113, 347, 366, 1492, 1844, 2004, 2045, 2053, 2079, 2134, 2161, 2175, 2220, 2288, 2295, 2379, 2417, 2461, 2552, 2585, 2670, 2698, 2736, 2739, 2780, 2824, 2891, 3034, 3040, 3078, 3112, 3122, 3140, 3153, 3197, 3251, 3309, 3332, 3342, 3385, 3418, 3479, 3536, 3542, 3548, 3719, 3899, 3998, 4287], 'C3': [96, 349, 3721], 'position': [97, 116, 350, 369, 3722], 'acyl': [100, 119, 353, 372, 564, 568, 721, 1820, 1829, 3714, 3725, 4111, 4317, 4339], 'chain.': [101, 354, 4183], 'data': [103, 356, 3552, 3994, 4256, 4351, 4357, 4363], 'also': [104, 357, 1612, 1748, 4187], 'indicate': [105, 358], 'amide': [108, 361, 1130], 'group': [109, 362], 'ketone': [112, 365], 'C1': [115, 368], 'chain': [120, 373, 722, 3715, 3726, 4112, 4319], 'could': [123, 376, 774, 3463, 3938], 'be': [124, 177, 377, 430, 1485, 1639, 2938, 3217, 3238, 3257, 3940], 'important': [125, 378, 838, 3465], 'structural': [126, 379, 772, 1778, 3472, 3737], 'features': [127, 380, 1779], 'enzyme-substrate': [129, 382], 'interaction.': [130, 383], 'Surprisingly,': [131, 384, 3629], 'although': [132, 385], 'carrying': [133, 386], '104HX-': [135, 388], 'HXDH109': [136, 389], 'short': [137, 390, 1533], 'sequence': [138, 391, 1534], 'identical': [139, 392, 1538, 3791], 'zinc-binding': [142, 395], 'motif': [143, 200, 396, 453, 1542, 3576, 3582], 'several': [145, 398, 1544, 3891], 'groups': [146, 399, 678, 1106, 3351], 'metallohydrolytic': [148, 401], 'enzymes,': [149, 402, 1108, 2804], 'does': [151, 242, 404, 495, 2896], 'not': [152, 176, 243, 405, 429, 496, 1683, 2958, 3157, 3803, 3808, 3939, 4117, 4213, 4370, 4383], 'contain': [153, 406], 'require': [155, 408], 'zinc': [156, 246, 409, 499, 3643], 'other': [158, 195, 248, 411, 448, 501, 1688, 2519, 2960, 3318], 'metal': [159, 249, 412, 502, 1734, 2434, 2482, 3296, 3622, 3631], 'ions': [160, 250, 413, 503, 1689, 1743, 2435], 'for': [161, 165, 179, 251, 414, 418, 432, 504, 1025, 1432, 1641, 1692, 1762, 1841, 2091, 2223, 2414, 2464, 2528, 3012, 3127, 3150, 3486, 3636, 3956, 4005, 4090], 'activity.': [163, 220, 252, 416, 473, 505, 3133, 3396, 3488], 'Except': [164, 417], 'amino': [167, 420, 1601, 2885, 3458], 'acid': [168, 421, 1602, 2886, 3459], 'His-104,': [170, 423], 'was': [172, 425, 1813, 1849, 2002, 2050, 2084, 2098, 2148, 2170, 2199, 2218, 2233, 2302, 2317, 2345, 2366, 2410, 2425, 2471, 2544, 2596, 2612, 2623, 2639, 2668, 2765, 2791, 2833, 2861, 2900, 2910, 3015, 3053, 3064, 3082, 3097, 3101, 3145, 3167, 3202, 3214, 3254, 3321, 3633, 3645, 3743, 3753, 3777, 3870, 3991, 4011, 4033, 4059, 4081, 4238], 'shown': [173, 426, 3188, 3446], 'previously': [174, 427, 2248, 2645], 'required': [178, 431, 1691, 3978], 'catalysis,': [180, 433], 'kinetic': [181, 434, 1763], 'study': [182, 435, 2538], 'conformational': [184, 213, 437, 466, 1766, 3501, 3514, 3520], 'using': [186, 439, 1768, 1870, 1928, 2243, 2531, 2568, 2603, 2615, 2641, 3017, 3169, 3910], 'circular': [187, 440, 1769], 'dichroism': [188, 441, 1770], 'spectrometry': [189, 442, 1771, 2501, 3492], 'key': [196, 449, 1004], 'residues': [197, 450, 2887, 3460], '(His-106,': [201, 454], 'Asp-108,': [202, 455, 1605], 'His-109),': [204, 457], 'as': [205, 207, 458, 460, 554, 662, 828, 859, 1021, 1793, 2324, 2347, 2427, 2455, 2950, 2952, 3019, 3087, 3171, 3187, 3380, 3686, 3745, 3798, 3823], 'well': [206, 459, 1491], 'His-169': [208, 461], 'with': [209, 462, 668, 1048, 1854, 2073, 2129, 2201, 2226, 2452, 2589, 2625, 2687, 2913, 2942, 2982, 3024, 3355, 3414, 3555, 3653, 3668, 4023, 4275, 4315, 4336], 'serine,': [210, 463], 'respectively,': [211, 464, 1124, 1925, 2786, 4286], 'caused': [212, 465], 'changes': [214, 467], 'significant': [216, 469], 'loss': [217, 470], 'We': [221, 474, 3489], 'conclude': [222, 475], 'is': [225, 478, 552, 830, 1537, 1611, 1682, 1699, 2976, 2980, 3137, 3410, 3484, 3608, 3658, 4205], 'highly': [227, 480], 'specific': [228, 481, 528], '106HXDH109∼H169': [233, 486], 'represents': [236, 489], 'novel': [238, 491, 1027], 'catalytic': [239, 492, 1694, 1715, 1784, 3165, 3481, 4129, 4266, 4309], 'motif,': [240, 493], 'rely': [244, 497], 'on': [245, 498, 1744, 2179, 2363, 2440, 2489, 2547, 2759, 3163, 3306, 3394, 3499, 3512, 3987], 'Many': [506], 'host-associated': [507], 'bacteria': [508], 'produce,': [509], 'release,': [510], 'respond': [512], 'small': [514], 'signal': [515, 2527], 'molecules': [516, 783], 'monitor': [518], 'their': [519, 669, 2180, 4342], 'own': [520], 'population': [521, 535], 'density': [522, 2039], 'control': [524, 1439, 1975], 'expression': [526, 846, 1010], 'genes': [529], 'response': [531], 'change': [533], 'density.': [536], 'This': [537], 'type': [538], 'gene': [540, 675, 845, 1009, 1907, 1972, 3409], 'regulation,': [541], 'controls': [543, 3059], 'functions': [546], 'virulence': [548, 844, 1008, 1303, 1307], 'biofilm': [550], 'formation,': [551], 'known': [553, 1700, 4188], '(QS)': [556], '1The': [557], 'abbreviations': [558], 'used': [559, 3490, 3744], 'are:': [560], 'QS,': [561], 'quorum-sensing;': [562], 'AHL,': [563], 'lactone;': [566], 'Acyl-HS,': [567], 'homoserine;': [569], 'HPLC,': [570], 'high': [571, 4100], 'pressure': [572, 2077], 'liquid': [573], 'chromatography;': [574], 'GST,': [575], 'glutathione': [576, 1969], 'S-transferase;': [577], 'IPTG,': [578], 'isopropyl': [579, 1978], 'β-d-thiogalactopyranoside;': [580], 'PBS,': [581], 'phosphate-buffered': [582], 'saline;': [583], 'ICP-MS,': [584], 'inductively': [585, 2497], 'coupled': [586, 2498], 'plasma': [587, 2499], 'mass': [588, 1889, 2241, 2500, 2986, 3797], 'spectroscopy;': [589], 'HSL,': [590], 'lactone.': [592], '(1Fuqua': [593, 730], 'C.': [594, 731, 979, 1213, 1219, 1345, 1351, 1549, 1572, 3831, 3847], 'Parsek': [595, 732], 'M.R.': [596, 733], 'Greenberg': [597, 734, 940, 1263], 'E.P.': [598, 735, 941, 1264], 'Ann.': [599, 736], 'Rev.': [600, 619, 637, 737, 748, 766], 'Genet.': [601, 738], '2001;': [602, 621, 638, 739, 750, 767, 1172, 1340, 1422, 1469, 1524, 2262, 2339], '35:': [603, 740], '439-468Google': [604, 741], 'Scholar,': [605, 613, 624, 742, 753, 801, 898, 913, 927, 952, 964, 975, 987, 1085, 1158, 1175, 1192, 1211, 1240, 1260, 1270, 1326, 1343, 1372, 1472, 1562, 1582, 1663, 3841], '2Winans': [606], 'S.C.': [607], 'Trends': [608, 1475, 1556], 'Microbiol.': [609, 620, 636, 749, 765, 971, 997, 1188, 1207, 1256, 1388, 1676], '1998;': [610, 998, 1559], '6:': [611], '382-383Google': [612], '3Miller': [614, 743], 'B.': [615, 744, 868, 1551, 3845], 'Bassler': [616, 745], 'B.L.': [617, 746], 'Annu.': [618, 747], '55:': [622, 751], '165-199Google': [623, 752], '4Whitehead': [625, 754], 'N.D.': [626, 755], 'Barnard': [627, 756], 'A.M.L.': [628, 757], 'Slater': [629, 758], 'H.': [630, 759, 1231, 1363, 3861], 'Simpson': [631, 760], 'N.J.L.': [632, 761], 'Salmond': [633, 762, 794, 887, 992, 3832], 'G.P.C.': [634, 763, 795, 888, 3833], 'FEMS': [635, 764], '25:': [639, 768], '365-404Google': [640, 769], 'Scholar).': [641, 770, 814, 1001, 1104, 1287, 1392, 1425, 1481, 1527, 1596, 1680, 1954, 2265, 2342, 2657, 3008, 3375, 3438, 3603, 3930], 'In': [642, 1708, 3204, 3931, 4150], 'general,': [643], 'each': [644, 3973], 'individual': [645], 'cell': [647, 2078], 'produces': [648], 'basal': [650], 'level': [651, 3682], 'QS': [653, 680, 695, 817, 1050, 1398, 1454], 'signals.': [654], 'accumulate': [657], 'threshold': [660], 'concentration': [661, 2032, 2169, 2311, 2673, 3387, 3901, 3971, 4004, 4015, 4037], 'cells': [664], 'proliferate': [665], 'interact': [667], 'cognate': [670], 'transcription': [671, 1059], 'factors': [672, 1304], 'activate': [674], 'expression.': [676], 'Several': [677, 1033, 3295, 4184], 'have': [682, 1036, 1636, 1712, 1747, 1759], 'been': [683, 1019, 1037, 1749, 1760, 2749, 3809], 'identified.': [684], 'Among': [685], 'them,': [686], '(AHLs)': [690], 'comprise': [691], 'family': [693], 'identified': [697, 1038, 1135], 'many': [699], 'Gram-negative': [700], 'bacteria,': [701, 1013], 'particular,': [703, 842], 'Proteobacteria.': [704], 'Different': [705], 'species': [707], 'may': [708], 'different': [710, 2000, 2453, 3701, 4163, 4273], 'vary': [713], 'maintain': [724], 'same': [726, 2245, 2370, 2769, 3068, 3681], 'moiety': [729], 'These': [771, 1426, 1863, 3260, 3710], 'variations': [773], 'constitute': [775], 'basis': [777], 'signaling': [779, 1016, 1051, 1399], 'AHL': [782, 1015, 1042, 1121, 1497, 1723, 2190, 2202, 2316, 2352, 2678, 2716, 3063, 3690, 3708, 3742, 3752, 3877, 3945, 3962, 3974], '(5Welch': [784], 'M.': [785, 872, 915, 1062, 1067, 1087, 1091, 1099, 1225, 1357, 1570, 3835, 3855, 3859], 'Todd': [786], 'D.E.': [787], 'Whitehead': [788], 'N.A.': [789], 'McGowan': [790], 'S.J.': [791, 1194], 'Bycroft': [792, 873], 'B.W.': [793, 874], 'EMBO': [796, 893, 922, 1577], 'J.': [797, 894, 923, 959, 970, 982, 1080, 1246, 1265, 1378, 1578], '2000;': [798, 1155, 1267, 1323, 1632, 1660, 1951, 3005, 3435, 3600], '19:': [799], '631-641Google': [800], '6Zhang': [802], 'L.H.': [803, 1147, 1162, 1170, 1185, 1248, 1254, 1274, 1276, 1315, 1330, 1338, 1380, 1386, 1412, 1420, 1459, 1467, 1474, 1514, 1522, 1624, 1652, 1673, 1796, 1943, 2252, 2260, 2329, 2337, 2997, 3427, 3592], 'Murphy': [804, 1797], 'P.J.': [805, 882, 1798], 'Kerr': [806, 1799], 'A.': [807, 937, 948, 1154, 1283, 1322, 1553, 1564, 1631, 1659, 1800, 1950, 3004, 3434, 3599, 3912], 'Tate': [808, 1801], 'M.E.': [809, 1802], 'Nature.': [810, 1171, 1339, 1421, 1468, 1523, 1803, 2261, 2338, 3926], '1993;': [811, 895, 910, 924, 1804, 3927], '362:': [812, 1805, 3928], '446-448Google': [813, 1806], 'AHL-dependent': [816, 1058], 'system': [818, 1017], 'has': [819, 1018, 2882, 3508, 4126, 4178], 'drawn': [820], 'considerable': [821], 'attention': [822], 'over': [823], 'last': [825], '10': [826, 2224, 2792, 3128, 3707, 3768, 3904, 4091], 'years,': [827], 'it': [829, 3607, 3657], 'involved': [831], 'regulation': [834], 'range': [849, 1721, 2558, 3206, 4016, 4038, 4069, 4270], 'animal': [851], '(including': [852], 'human)': [853], 'plant': [855], 'pathogens': [857, 1450], 'such': [858, 3379, 3822], 'Erwinia': [860], 'carotovora': [861, 1291, 1407], 'Pseudomonas': [863], 'aeruginosa': [864, 1294], '(7Jones': [865], 'S.': [866, 884, 947, 956, 995, 1075, 1079, 1097, 1153, 1282, 1321, 1630, 1658, 1949, 3003, 3433, 3598, 3849, 3923], 'Yu': [867], 'Bainton': [869], 'N.J.': [870], 'Birdsall': [871], 'Chhabra': [875, 3850], 'S.R.': [876, 3851], 'Cox': [877, 3363], 'A.J.R.': [878], 'Golby': [879], 'P.': [880, 892, 1095, 1221, 1293, 1353, 3863], 'Reeves': [881], 'Stephens': [883], 'Winson': [885], 'M.K.': [886, 1586], 'Stewart': [889], 'G.S.A.B.': [890], 'Williams': [891, 3862], '12:': [896, 925], '2477-2482Google': [897], '8Passador': [899], 'L.': [900, 906, 933, 1069, 1073, 1217, 1349, 1588], 'Cook': [901], 'J.M.': [902, 966, 1574], 'Gambello': [903], 'M.J.': [904], 'Rust': [905], 'Iglewski': [907, 938], 'B.H.': [908, 939], 'Science.': [909], '260:': [911], '1127-1130Google': [912], '9Pirhonen': [914], 'Flego': [916], 'D.': [917, 1235, 1367], 'Heikinheimo': [918], 'R.': [919, 1065, 1071, 1555], 'Palva': [920], 'E.': [921, 981, 1290, 1406, 1568, 2864], '2467-2476Google': [926], '10Pearson': [928], 'J.P.': [929], 'Gray': [930], 'K.M.': [931], 'Passador': [932], 'Tucker': [934], 'K.D.': [935], 'Eberhard': [936], 'Proc.': [942, 1148, 1277, 1316, 1625, 1653, 1944, 2998, 3428, 3593], 'Natl.': [943, 1149, 1278, 1317, 1626, 1654, 1945, 2999, 3429, 3594], 'Acad.': [944, 1150, 1279, 1318, 1627, 1655, 1946, 3000, 3430, 3595], 'Sci.': [945, 1151, 1280, 1319, 1477, 1558, 1628, 1656, 1947, 3001, 3431, 3596], 'U.': [946, 1152, 1281, 1320, 1629, 1657, 1948, 3002, 3432, 3597], '1994;': [949], '91:': [950], '197-201Google': [951], '11Beck': [953], 'von': [954, 3921], 'Bodman': [955, 3922], 'Farrand': [957, 3924], 'S.K.': [958, 3925], 'Bacteriol.': [960, 983, 1081, 1266, 3837], '1995;': [961, 1579], '177:': [962], '5000-5008Google': [963], '12Costa': [965], 'Loper': [967], 'J.E.': [968], 'Can.': [969], '1997;': [972, 984, 1593], '43:': [973], '1164-1171Google': [974], '13Dunphy': [976], 'G.': [977, 993, 1233, 1365], 'Miyamoto': [978], 'Meighen': [980], '179:': [985], '5288-5291Google': [986], '14Nasser': [988], 'W.': [989], 'Bouillant': [990], 'M.L.': [991], 'Reverchon': [994], 'Mol.': [996, 1255, 1387], '29:': [999], '1391-1405Google': [1000], 'Being': [1002], 'attribute': [1005], 'determines': [1007], 'pathogenic': [1012], 'regarded': [1020], 'promising': [1023, 1430], 'target': [1024], 'developing': [1026], 'approaches': [1028], 'controlling': [1030], 'infections.': [1032], 'anti-QS': [1034], 'mechanisms': [1035], 'recent': [1040], 'years.': [1041], 'antagonists': [1043], 'found': [1045, 3778], 'interfere': [1047], 'inducing': [1053], 'accelerated': [1054], 'degradation': [1055], 'factor': [1060], '(15Givskov': [1061], 'de': [1063, 4384], 'Nys': [1064], 'Manefield': [1066], 'Gram': [1068], 'Maximilien': [1070], 'Eberl': [1072], 'Molin': [1074], 'Steinberg': [1076, 1094], 'P.D.': [1077], 'Kjelleberg': [1078, 1096], '1996;': [1082], '178:': [1083], '6618-6622Google': [1084], '16Manefield': [1086], 'Rasmussen': [1088], 'T.B.': [1089], 'Henzter': [1090], 'Andersen': [1092], 'J.B.': [1093], 'Givskov': [1098], 'Microbiology.': [1100, 1236, 1368], '2002;': [1101, 1189, 1208, 1237, 1284, 1369, 1677, 3838, 3866], '148:': [1102, 1238, 1370], '1119-1127Google': [1103], 'Two': [1105], 'i.e.': [1109], 'acyl-homoserine': [1111, 1115], 'lactonase': [1112], '(AHL-lactonase)': [1113], 'acylase': [1117], '(AHL-acylase),': [1118], 'degrade': [1120], 'hydrolyzing,': [1123], 'linkage': [1131], '(Fig.': [1132, 2963, 3043, 3314, 3563, 3727, 4168, 4231], '1),': [1133], 'numerous': [1137], 'isolates': [1139], '(17Dong': [1140, 1308, 1617, 1645, 1936, 2990, 3420, 3585], 'Y.H.': [1141, 1160, 1177, 1242, 1309, 1328, 1374, 1410, 1457, 1512, 1618, 1646, 1665, 1937, 2250, 2327, 2991, 3421, 3586], 'Xu': [1142, 1163, 1182, 1243, 1310, 1331, 1375, 1413, 1460, 1515, 1619, 1647, 1670, 1938, 2253, 2330, 2992, 3422, 3587], 'J.L.': [1143, 1164, 1183, 1244, 1311, 1332, 1376, 1414, 1461, 1516, 1620, 1648, 1671, 1939, 2254, 2331, 2993, 3423, 3588], 'Li': [1144, 1312, 1621, 1649, 1940, 2994, 3424, 3589], 'X.Z.': [1145, 1313, 1622, 1650, 1941, 2995, 3425, 3590], 'Zhang': [1146, 1165, 1167, 1169, 1180, 1184, 1253, 1275, 1314, 1333, 1335, 1337, 1385, 1415, 1417, 1419, 1462, 1464, 1466, 1517, 1519, 1521, 1623, 1651, 1668, 1672, 1942, 2255, 2257, 2259, 2332, 2334, 2336, 2996, 3426, 3591], '97:': [1156, 1324, 1633, 1661, 1952, 3006, 3436, 3601], '3526-3531Google': [1157, 1325, 1634, 1662, 1953, 3007, 3437, 3602], '18Dong': [1159, 1327], 'Wang': [1161, 1247, 1273, 1329, 1379, 1411, 1458, 1513, 2251, 2328], 'H.B.': [1166, 1272, 1334, 1416, 1463, 1518, 2256, 2333], 'X.F.': [1168, 1336, 1418, 1465, 1520, 2258, 2335], '411:': [1173, 1341, 1423, 1470, 1525, 2263, 2340], '813-817Google': [1174, 1342, 1424, 1471, 1526, 2264, 2341], '19Dong': [1176, 1664], 'Gusti': [1178, 1666], 'A.R.': [1179, 1667], 'Q.': [1181, 1669], 'Appl.': [1186, 1205, 1674], 'Environ.': [1187, 1206, 1675], '68:': [1190, 1209, 1678], '1754-1759Google': [1191, 1679], '20Lee': [1193], 'Park': [1195], 'S.Y.': [1196], 'Lee': [1197, 1203], 'J.J.': [1198], 'Yum': [1199], 'D.Y.': [1200], 'Koo': [1201], 'B.T.': [1202], 'J.K.': [1204], '3919-3924Google': [1210], '21Reimmann': [1212, 1344], 'Ginet': [1214, 1346], 'N.': [1215, 1347], 'Michel': [1216, 1348], 'Keel': [1218, 1350], 'Michaux': [1220, 1352], 'Krishnapillai': [1222, 1354], 'V.': [1223, 1355], 'Zala': [1224, 1356], 'Heurlier': [1226, 1358], 'K.': [1227, 1229, 1359, 1361, 4196], 'Triandafillu': [1228, 1360], 'Harms': [1230, 1362], 'Defago': [1232, 1364], 'Haas': [1234, 1366], '923-932Google': [1239, 1371], '22Lin': [1241, 1373], 'Hu': [1245, 1377], 'Ong': [1249, 1381], 'S.L.': [1250, 1382], 'Leadbetter': [1251, 1383], 'J.R.': [1252, 1262, 1384], '2003;': [1257, 1389, 1478], '47:': [1258, 1390], '849-860Google': [1259, 1391], '23Leadbetter': [1261], '182:': [1268], '6921-6926Google': [1269], '24Zhang': [1271], '99:': [1285], '4638-4643Google': [1286], 'Genetically': [1288], 'modified': [1289], 'expressing': [1295, 1394], 'AHL-acylase': [1298], 'decreased': [1300, 3146], 'production': [1301], 'attenuated': [1306], 'Plants': [1393], 'quenched': [1396], 'pathogen': [1397], 'significantly': [1402, 3540, 4118], 'enhanced': [1403, 3023], 'resistance': [1404], 'infection': [1408], '(18Dong': [1409, 1456, 1511, 2249, 2326], 'findings': [1427], 'highlight': [1428], 'potential': [1431, 3046], 'establishing': [1433], 'generic': [1435], '"quorum-quenching"': [1436], 'approach': [1437], 'infections,': [1441], 'is,': [1443], 'paralyze': [1445], 'through': [1451], 'inactivation': [1452, 3201], 'systems': [1455], '25Zhang': [1473], 'Plant': [1476], '8:': [1479], '238-244Google': [1480], 'appears': [1483], 'potent': [1487], 'enzyme.': [1488, 2633, 3073, 3689], 'It': [1489, 1681], 'works': [1490], 'physiologically': [1493], 'relevant': [1494], 'concentrations': [1495, 2777, 2837], 'hydrolyzes': [1500], '(C4-HSL,': [1505], '3-oxo-C6-HSL,': [1506], '3-oxo-C8-HSL,': [1507], '3-oxo-C12-HSL)': [1509], 'effectively': [1510], 'contains': [1530, 3574], 'conserved': [1532, 1613], '104HXHXDH109,': [1535], 'Zn2+-binding': [1541], 'metallohydrolases': [1545, 1616], '(26Melino': [1546], 'S': [1547, 1566], 'Capo': [1548], 'Dragani': [1550], 'Aceto': [1552], 'Petruzzelli': [1554], 'Biochem.': [1557], '23:': [1560], '381-382Google': [1561], '27Carfi': [1563], 'Pares': [1565], 'Duee': [1567], 'Galleni': [1569], 'Duez': [1571], 'Frere': [1573], 'Dideberg': [1575], 'O.': [1576], '14:': [1580], '4914-4921Google': [1581], '28Crowder': [1583], 'M.W.': [1584], 'Maiti': [1585], 'Banovic': [1587], 'Makaroff': [1589], 'C.A.': [1590], 'FEBS': [1591], 'Lett.': [1592], '418:': [1594], '351-354Google': [1595], 'Within': [1597, 4133, 4293], 'sequence,': [1599], 'three': [1600, 4000], 'residues,': [1603], 'His-106,': [1604], 'His-109,': [1607], 'plus': [1608], 'His-169,': [1609], 'Scholar),': [1635, 1807, 3869, 4203], 'proven': [1637], 'essential': [1640], 'clear': [1684], 'whether': [1685, 3606], 'Zn2+': [1686, 3580], 'function': [1695], 'AHL-lactonase.': [1697, 2358, 3517], 'Little': [1698], 'about': [1701], 'properties.': [1707], 'this': [1709, 3873], 'study,': [1710], 'we': [1711, 3611, 3703], 'investigated': [1713], 'against': [1719, 2598, 4067, 4140, 4268, 4333], 'derivatives': [1724, 4344], 'related': [1726], 'compounds.': [1727], 'probe': [1729], 'enzymatic': [1731, 3684], 'mechanism,': [1732], 'ion': [1735, 2483, 2530, 3623, 3632], 'composition': [1736, 2484], 'effect': [1741, 2360, 3160, 3305, 3393, 3496, 3511], 'determined.': [1750], 'Furthermore,': [1751], 'deficient': [1755], 'purified': [1761, 1869, 2154, 2195, 2870, 2911, 2973, 3119], 'assay': [1764], 'an': [1773, 2579, 3411, 3731, 3911], 'attempt': [1774], 'reveal': [1776, 2959], 'governing': [1780], 'substrate-enzyme': [1781], 'interaction': [1782], 'efficiencies.': [1785], 'Synthesis': [1786], 'Derivatives—AHLs': [1790], 'synthesized': [1792, 3704], 'described': [1794, 2247, 2325, 2428, 2477, 2644, 3088], '(6Zhang': [1795], 'except': [1808, 2372, 2771, 3635], '3-hydroxylbutanoyl': [1810], 'l-homoserine': [1811, 4043], 'purchased': [1814, 1894], 'Quorum': [1816], 'Sciences': [1817, 2493], 'Inc.': [1818], 'homoserines': [1821], '(Acyl-HSs)': [1822], 'prepared': [1824], 'incubating': [1826, 2447], '1:1': [1833], 'ratio': [1834, 3760, 4087], '1': [1836, 1855, 2206, 2675, 3957], 'm': [1837, 1856, 2210, 2278, 2384, 2682], 'NaOH/dimethyl': [1838], 'sulfoxide': [1839], '(v/v)': [1840], '12': [1842], 'h': [1843, 2416, 3152], 'room': [1845, 2135], 'temperature.': [1846, 2136], 'solution': [1848, 2403, 2449, 2679], 'neutralized': [1850], 'pH': [1852, 2362, 2389, 2392, 3011, 3025, 3035, 3041, 3100, 3498, 3507, 3527, 3537, 3543, 3549, 4288], '6.5': [1853], 'NaH2PO4': [1857], 'then': [1859, 2051, 2421, 3037], 'dried': [1860], 'under': [1861, 1973, 2473, 2564], 'vacuum.': [1862], 'synthetic': [1864], 'Acyl-HSs': [1867, 3806], 'silica': [1871], 'gel': [1872], 'column': [1873, 2105], 'chromatography': [1874, 2875], 'C18': [1876], 'reservephase': [1877], 'HPLC': [1878, 2237, 2320, 2712, 3784, 3792], 'confirmed': [1880, 3083, 3675], 'structurally': [1881, 4272], '1H': [1883], 'NMR': [1884], 'spectroscopy': [1885], 'electrospray': [1887, 2239], 'ionization': [1888, 2240], 'spectrometry.': [1890], 'Other': [1891], 'reagents': [1892, 2439, 2454, 3378], 'Sigma-Aldrich': [1896], 'unless': [1897], 'otherwise': [1898], 'stated.': [1899], 'Purification': [1900, 2853], 'Its': [1904], 'Variants—The': [1905], 'encoding': [1908], 'H106S,': [1913], 'D108S,': [1914], 'H109S,': [1915], 'H169S': [1917], 'contained': [1918], 'pGEM-7Zf(+)': [1921], 'vector': [1922, 1986], 'amplified,': [1924], 'PCR': [1927, 1956], 'forward': [1929], 'primer': [1930, 1934], '5′-ATCGGATCCATGACAGTAAAGAAGCTTTATTTCG-3′': [1931], 'reverse': [1933], '5′-GTCGAATTCCTCAACAAGATACTCCTAATGATGT-3′': [1935], 'products': [1957, 3892, 3964], 'digested': [1959], 'BamHI': [1961], 'EcoRI': [1963], 'fused': [1965], 'in-frame': [1966], 'S-transferase': [1970], '(GST)': [1971], 'β-d-thiogalactopyranoside': [1979], '(IPTG)-inducible': [1980], 'tac': [1981], 'promoter': [1982], 'GST': [1984, 2109, 2126, 2873, 2903], 'fusion': [1985, 2017, 2110, 2859], 'pGEX-2T': [1987], '(Amersham': [1988, 2106], 'Biosciences).': [1989, 2107], 'constructs': [1991, 2001], 'verified': [1993], 'DNA': [1995], 'sequencing.': [1996], 'Escherichia': [1997], 'coli': [1998, 2865], 'containing': [1999, 2011, 2141, 2272, 2807, 4345], 'cultured': [2003], '30': [2005, 2092, 2465], '°C': [2006, 2055, 2163, 2222, 2381, 2463, 2554, 3126, 3155, 3199, 3253], '5-liter': [2008], 'LB': [2009], 'medium': [2010], '100': [2012], 'μg·ml-1': [2013], 'ampicillin.': [2014], 'GST-AHL-lactonase': [2016], 'protein': [2018, 2860, 2961, 3413], 'expressed': [2023, 2862], 'addition': [2026], 'IPTG': [2028, 2867], 'final': [2031, 2310, 2672, 2689, 2842], '0.5': [2034, 2784], 'mm': [2035, 2782, 3334, 3390, 3905], 'after': [2036, 3148, 3779, 3952], 'optical': [2038], 'culture': [2042, 2049], 'reached': [2043, 3031], '0.4–0.5': [2044], '600': [2046], 'nm;': [2047], 'incubated': [2052, 2219, 2284, 2378, 2697, 3065, 4089], '28': [2054, 2221], 'overnight.': [2056], 'cells,': [2058], 'harvested': [2059], 'centrifugation': [2061, 2087], 'resuspended': [2063], '1×': [2065, 2405, 2458, 2626], 'PBS': [2066, 2406, 2459, 2627], 'buffer': [2067, 2212, 2280, 2386, 2407, 2460, 2628, 2684, 3070], '(pH': [2068, 2213, 2281, 2408, 2685], '7.4),': [2069], 'disrupted': [2071], 'twice': [2072], 'chilled': [2075], 'French': [2076], '2000': [2080], 'p.s.i.': [2081], 'Cell': [2082], 'debris': [2083], 'removed': [2085], '(11,000': [2088], '×': [2089, 2826], 'g': [2090, 2827], 'min,': [2093], '4': [2094, 3123, 3504], '°C).': [2095], 'supernatant': [2097], 'added': [2099, 2669], 'glutathione-Sepharose': [2102], '4B': [2103], 'affinity': [2104, 2116, 2874, 4301], 'proteins': [2111], 'bound': [2113], 'matrix,': [2117], 'separated': [2124, 2901], 'digestion': [2128], 'protease': [2131], 'thrombin': [2132, 2905], 'overnight': [2133], 'After': [2137, 2229], 'digestion,': [2138], 'eluates': [2140], 'combined,': [2144], 'purity': [2147, 2928], 'analyzed': [2149, 2235, 2602, 3168], '10%': [2151, 2306, 2707, 2744], 'SDS-PAGE.': [2152], 'stored': [2160], '-80': [2162], 'prior': [2164], 'use.': [2166], 'determined': [2171, 2367, 2488, 2735, 2766, 4239, 4371], 'UV': [2173], 'spectrophotometry': [2174], '280': [2176], 'nm': [2177, 2563], 'based': [2178, 2758], 'molar': [2182, 3759, 4086], 'extinction': [2183], 'coefficients': [2184], '(for': [2185], 'example,': [2186], 'ϵAHL-lactonase)=': [2187], '18970': [2188], 'm-1·cm-1.': [2189], 'Hydrolysis': [2191, 3691], 'Product': [2193], 'Analysis—The': [2194, 2269], '(0.4': [2197], 'μm)': [2198, 2451], 'mixed': [2200, 4082], '(1': [2203], 'mm)': [2204], 'ml': [2207], '0.1': [2209, 2277, 2383, 2953], 'phosphate': [2211, 2279, 2385, 2683, 4380], '8),': [2214], 'mixture': [2217, 2232], 'min': [2225, 3769], 'gentle': [2227], 'shaking.': [2228], 'incubation,': [2230], 'immediately': [2234], 'spectrometry,': [2242], 'conditions': [2246, 2476, 2521], 'Enzyme': [2267, 2658], 'Property': [2268], 'reaction': [2270, 2301, 2789, 3069, 3348, 3782], 'mixtures': [2271, 2376], '7.4)': [2282, 2409, 2686], 'water': [2286], 'baths': [2287], 'specified': [2290], 'temperatures.': [2291], 'Ali-quots': [2292], 'taken': [2294], 'various': [2296, 2418, 2433], 'time': [2297, 2592, 2737, 2790, 3794, 3874], 'points,': [2298], 'stopped': [2303, 2701], 'adding': [2305, 2703], 'SDS': [2307], '2%.': [2313], 'remaining': [2315, 2468], 'quantified': [2318, 2722, 2848], 'bioassay': [2322, 2851, 3989], 'defined': [2346], 'hydrolyzed': [2349, 3935], 'micromoles': [2350], 'per': [2353, 2355, 3648], 'minute': [2354], 'microgram': [2356], 'procedure,': [2371, 2770], 'hydrolysis': [2375, 2719, 3052, 3963, 3975, 4002, 4304], '22': [2380, 2462, 2553, 2699, 4291], 'ranging': [2387, 3528, 4280], '5': [2390, 3104, 4094], '9.': [2393], 'examine': [2395], 'thermal': [2397, 3110], 'stability': [2398, 3111], 'allowed': [2411], 'stand': [2413], '2': [2415, 3151, 3333, 3389], 'temperatures,': [2419], 'residual': [2423, 2715, 4155], 'measured': [2426, 2472, 3612], 'above.': [2429, 2478], 'effects': [2431], 'divalent': [2437], 'metal-chelating': [2438], 'examined': [2445, 3016], '(3': [2450], 'indicated': [2456], 'min.': [2466, 2793, 4092], 'standard': [2475, 2532], 'Metal': [2479], 'Ion': [2480], 'Determination—The': [2481], 'PerkinElmer': [2491], 'Life': [2492], 'SCIEX': [2494], 'ELAN': [2495], '6100': [2496], '(ICP-MS).': [2502], 'Mass': [2503], 'discrimination,': [2504], 'auxiliary': [2505], 'argon,': [2506], 'coolant': [2508], 'gas': [2509, 3292], 'flow': [2510], 'rates': [2511], 'controlled': [2513], 'automatically': [2514], 'instrument.': [2517], 'operating': [2520], 'adjusted': [2523, 3102], 'maximize': [2525], 'analyte': [2529], 'solutions.': [2533], 'Circular': [2534], 'Dichroism': [2535], 'Spectroscopy—Far-UV': [2536], 'CD': [2537, 3085, 3491], 'carried': [2545], 'out': [2546], 'JASCO': [2549], 'J-810': [2550], 'spectropolarimeter': [2551], 'wavelength': [2557], 'between': [2559, 3207, 3455], '185': [2560], '260': [2562], 'constant': [2565], 'nitrogen': [2566], 'flush,': [2567], '1-mm': [2569], 'path': [2570], 'quartz': [2572], 'cells.': [2573], 'spectra': [2575], 'derived': [2577], 'average': [2580], 'five': [2582], 'scans': [2583], 'recorded': [2584], '50': [2586], 'nm·min-1,': [2587], 'along': [2588], '1-s': [2591], 'constant.': [2593], 'Each': [2594], 'spectrum': [2595, 4130], 'corrected': [2597, 2624], 'blank,': [2599], 'smoothed,': [2600], 'software': [2605], 'package': [2606], 'provided': [2607], 'JASCO.': [2609], 'instrument': [2611], 'regularly': [2613], 'calibrated': [2614], 'ammonium': [2616], 'd-(+)-10-camphorsulfonate': [2617], 'following': [2618, 2866], "manufacturer's": [2620], 'recommendations.': [2621], 'Baseline': [2622], 'absence': [2631], 'fraction': [2635, 3776, 3788], 'secondary': [2637], 'structure': [2638, 3515, 3521], 'estimated': [2640], 'method': [2643], '(29Yang': [2646], 'J.T.': [2647, 3829], 'Wu': [2648], 'C.-S.C.': [2649], 'Martinez': [2650], 'H.M.': [2651], 'Methods': [2652], 'Enzymol.': [2653], '1986;': [2654], '130:': [2655], '208-269Google': [2656], 'Kinetics': [2659], 'Specificity': [2661, 4052], 'AHL-lactonase—To': [2663, 3693], 'determine': [2664, 2797, 3494, 3605], 'kinetics,': [2666], 'μm': [2676, 2809, 2813], '(0.3–20': [2680], 'mm)in0.1': [2681], 'volume': [2690, 2830], '96': [2692], 'μl.': [2693, 2835], 'reactions': [2695], '°C,': [2700, 3116, 3179, 3209], '24': [2704], 'μl': [2705], 'SDS,': [2708], 'subjected': [2710], 'analysis.': [2713, 2852, 3785], 'product': [2720, 3976], 'HPLC.': [2724], 'All': [2725], 'experiments': [2726], 'performed': [2728], 'triplicate,': [2730], 'velocities': [2733], 'points': [2738], 'more': [2742], 'than': [2743, 2895, 3749, 4145, 4320, 4341], 'consumed.': [2750], 'kcat': [2752, 4247, 4276, 4329], 'Km': [2754, 4249, 4278], 'values': [2755, 4250, 4279, 4332], 'calculated': [2757, 3215, 3236, 3255, 3264, 4252], 'Michaelis-Menten': [2760, 4259], 'equation.': [2761], 'fixed': [2779], '3': [2781], 'μm,': [2785], 'Substrate': [2794, 4051], 'Binding': [2795], 'Assay—To': [2796], 'binding': [2800, 3581], 'ability': [2801, 3733], '2-ml': [2805], 'solutions': [2806], '20': [2808], '10–200': [2812], '3-oxo-C8-HSL': [2814, 2839, 3018, 3170], 'transferred': [2816], 'Centricon-10': [2818], 'tubes': [2819], '(Amicon,': [2820], 'Millipore)': [2821], 'centrifuged': [2823], '5000': [2825], 'until': [2828], 'concentrate': [2832, 2843], '∼60–80': [2834], 'filtrate': [2846], 'separately': [2849], 'Properties': [2855], 'AHL-lactonase—The': [2857, 4054], 'GST-AiiA': [2858], 'induction': [2868], 'routine': [2872], 'procedure.': [2876], 'AiiA': [2879], '(AHL-lactonase),': [2880], 'two': [2883], 'extra': [2884], '(Gly': [2888], 'Ser)': [2890], 'N': [2893], 'terminus': [2894], 'native': [2898], 'AiiA,': [2899], 'digestion.': [2906], '86-fold': [2912], 'yield': [2915], '∼7.3%': [2917], 'total': [2920], 'proteins.': [2921], 'SDS-PAGE': [2923, 2966], 'indicates': [2925, 4007, 4029, 4369, 4382], 'obtained': [2931], '(7': [2934], 'μg': [2935, 2954], 'loaded)': [2936], 'should': [2937], '>98.5%,': [2939], 'because': [2940], 'staining': [2941], 'Coomassie': [2943], 'Brilliant': [2944], 'Blue': [2945], 'R-250,': [2946], 'can': [2948], 'detect': [2949], 'protein,': [2956, 3651], 'did': [2957, 4116, 4146, 4212], 'bands': [2962], '2).': [2964], 'size': [2970], '∼28': [2977], 'kDa,': [2978], 'consistent': [2981, 3554], 'predicted': [2984], 'molecular': [2985, 3796], '28,036': [2988], 'Da': [2989], 'optimal': [3010], 'substrate.': [3020, 3172, 3747, 4209], 'activity,': [3022, 3889, 4122], 'increasing': [3026], '6': [3028], '8,': [3030, 3036], 'maximum': [3033, 3176], 'declined': [3038], 'slightly': [3039, 3534], '9': [3042, 3532], '3A).': [3044, 3564], 'interference': [3047], 'non-enzymatic': [3049, 3818], 'pH-dependent': [3050, 3440, 3557, 3824], 'precluded': [3054], 'without': [3071, 4142], 'appeared': [3076], 'unstable': [3077], 'low': [3079], 'pH,': [3080], 'next': [3091], 'section;': [3092], 'detected': [3098, 3810, 4012, 4034], 'when': [3099, 3908], 'below.': [3106], 'exhibited': [3108, 4099], 'excellent': [3109, 3732], 'temperatures': [3113], 'below': [3114], '37': [3115, 3178], 'kept': [3121], '21': [3125], 'days,': [3129], 'still': [3130], 'maintained': [3131, 3679], '>99%': [3132], 'But': [3134], 'less': [3138], 'stable': [3139], 'higher': [3141, 4300, 4328], 'temperatures;': [3142], 'sharply': [3147], 'incubation': [3149, 3955], '>45': [3154], '(data': [3156, 3802], 'shown).': [3158, 3804], 'temperature': [3162, 3185], 'Up': [3173], 'typical': [3184], 'dependence': [3186], 'Arrhenius': [3191], 'plot': [3192], 'Fig.': [3194, 3448, 3503, 4093], '3B,': [3195], 'whereas': [3196], '45': [3198], 'noticed.': [3203], '6–37': [3208], 'activation': [3211, 3234, 3250], 'energy': [3212, 3247], 'Ea': [3213, 3273], '52.4': [3218], 'kJ·mol-1': [3219, 3240], 'slope': [3222], '(Ea/R)': [3223], 'graph.': [3226], 'enthalpy': [3228], 'ΔH*': [3229, 3271], 'entropy': [3231], 'ΔS*': [3232, 3277], '49.9': [3239], '-53.7': [3242], 'J·mol-1·K-1,': [3243], 'respectively.': [3244, 3294], 'free': [3246], 'ΔG*': [3248, 3268], '25': [3252], '65.9': [3258], 'kJ·mol-1.': [3259], 'thermodynamic': [3261], 'parameters': [3262], 'equations': [3267], '=': [3269, 3272, 3278], '-RTln(kcath/kBT),': [3270], '-': [3274, 3280], 'RT,': [3275], '(ΔH*': [3279], 'ΔG*)/T,': [3281], 'where': [3282], 'kB,': [3283], 'h,': [3284], 'R': [3286], 'Boltzmann,': [3288], 'Plank,': [3289], 'universal': [3291], 'constants,': [3293], 'ions,': [3297], 'Mg2+,Ca2+,Mn2+,Co2+,Ni2+,': [3299], 'Zn2+,': [3300, 3619], 'Cd2+,': [3302], '0.2': [3310, 3343], '2mm,': [3312], 'respectively': [3313], '3C).': [3315], 'On': [3316], 'hand,': [3319], 'partially': [3322], 'inhibited': [3323, 3337], 'Cr2+': [3325], '(72%),': [3326], 'Pb2+': [3327, 3621], '(67%),': [3328], 'Fe2+': [3330], '(48%)': [3331], 'completely': [3336, 3546], 'Cu2+': [3339, 3356], 'Ag+': [3341, 3358], 'mm,': [3344, 4285], 'possibly': [3345], 'due': [3346, 4372], 'sulfhydryl': [3350], '(30Lehninger': [3359], 'A.L.': [3360], 'Nelson': [3361], 'D.L.': [3362], 'M.M.': [3364], 'Principles': [3365], 'Biochemistry,': [3367], '2nd': [3368], 'Ed.': [3369], 'Worth': [3370], 'Publishers,': [3371], 'New': [3372], 'York,1993:': [3373], '198-239Google': [3374], 'chelating': [3377, 3669], 'EDTA,': [3381], '2,2′-bipyridine,': [3382], 'o-phenanthroline': [3384], 'Conformational': [3398], 'Structure': [3399], 'Is': [3402, 3566], 'pH-dependent—The': [3403], 'acidic': [3412], 'isoelectric': [3416], 'point': [3417], '4.7': [3419], 'pattern': [3441, 3560], '3A': [3449], 'suggests': [3450], 'electrostatic': [3453, 3477], 'interactions': [3454], 'charged': [3457], 'play': [3464], 'roles': [3466], 'maintenance': [3468], 'overall': [3471], 'conformation': [3473], 'local': [3476], 'potentials': [3478], 'center,': [3482], 'critical': [3485], 'structure.': [3502], 'shows': [3505, 4095], 'drastic': [3510], 'asymmetric': [3519], 'remained': [3524], 'unchanged': [3525], '7': [3530], 'changed': [3535, 3541], '6,': [3538], '5.5': [3544], 'lost': [3547], '5.': [3550], 'Not': [3567], 'Metalloenzyme—Sequence': [3569], 'alignment': [3570], 'suggested': [3571], 'similar': [3577], 'metalloenzymes': [3584], 'metalloenzyme,': [3610], 'Mg2+,': [3613], 'Ca2+,': [3614], 'Mn2+,': [3615], 'Fe2+,': [3616], 'Co2+,': [3617], 'Cu2+,': [3618], 'contents': [3624], 'ICP-MS.': [3628], 'detectable': [3634], 'trace': [3638], 'mount': [3639], 'Zn2+.': [3641], 'content': [3644], '∼0.08': [3646], 'mol': [3647, 3649], 'inconsistent': [3652], 'notion': [3655], 'metallohydrolase.': [3660], 'Moreover,': [3661], 'zinc-free': [3663], 'generated': [3665], 'treatment': [3667], 'reagent': [3670], 'EDTA': [3671], 'dialysis': [3673], 'ICP-MS': [3677], 'analysis,': [3678], 'untreated': [3688], 'compare': [3694], 'molecules.': [3709], 'differ': [3712], '5).': [3728, 4169, 4232], 'accommodate': [3735], 'these': [3736, 4294], 'differences': [3738], 'regardless': [3739], 'More': [3748], '80%': [3750], 'degraded': [3754], '1:2500': [3758], 'first': [3767], 'reaction,': [3771], 'only': [3773, 4154], 'one': [3774], 'new': [3775, 3787, 4048], 'retention': [3793], 'Acyl-HS': [3801], 'enzyme-free': [3813], 'control,': [3814], 'indicating': [3815, 4123], 'turnover': [3819], 'lactonolysis': [3825, 3878], 'alkalization': [3827], '(31Byers': [3828], 'Lucas': [3830], 'Welch': [3834], 'J': [3836], '184:': [3839], '1163-1171Google': [3840], '32Yates': [3842], 'E.A.': [3843], 'Philipp': [3844], 'Buckley': [3846], 'Atkinson': [3848], 'Sockett': [3852], 'R.E.': [3853], 'Goldner': [3854], 'Dessaux': [3856], 'Y.': [3857], 'Camara': [3858], 'Smith': [3860], 'Infect.': [3864], 'Immun.': [3865], '70:': [3867], '5635-5646Google': [3868], 'negligible': [3871], 'scale.': [3875], 'led': [3881], 'sharp': [3884], 'decrease': [3885], '(>1500-fold)': [3886], 'failed': [3893], 'show': [3895], 'any': [3896, 4215], 'even': [3898, 3951], 'up': [3902], '(Table': [3906, 4261, 4348], 'I)': [3907], 'assayed': [3909], 'tumefaciens': [3913], 'tra': [3914], 'gene-based': [3915], 'reporter': [3916], 'strain': [3917], '(33Piper': [3918], 'K.R.': [3919], 'Beck': [3920], '448-450Google': [3929], 'cases,': [3933], 'relactonized': [3941], 'form': [3943], 'active': [3944], 'neutral': [3948], 'aqueous': [3949], 'solution,': [3950], 'prolonged': [3954], 'week.Table': [3958], 'IBiological': [3959], 'Samples': [3965], 'diluted,': [3967], 'minimum': [3970], '(Acyl-HS)': [3977], 'induce': [3980], 'formation': [3981], 'visible': [3984], 'blue': [3985], 'colony': [3986], 'plate': [3990], 'presented.': [3992], 'means': [3996, 4353, 4359, 4365], 'least': [3999], 'repeats.AHL': [4001], 'productsMinimum': [4003], 'activityaND': [4006], '10-10,000': [4018, 4040], 'μmDecreased': [4019], '(times)': [4021], 'compared': [4022], 'AHLμm3-Oxo-C4-HSND3-Oxo-C6-HS50016673-Oxo-C8-HS5025003-Oxo-C10-HS30015003-Oxo-C12-HS10001667C4-HSNDC6-HSNDC8-HS10002000C10-HS200020003-HO-C4-HSb3-Hydroxylbutanoyl': [4026], 'l-homoserineNDa': [4027], 'ND': [4028], 'μmb': [4041], '3-Hydroxylbutanoyl': [4042], 'Open': [4044], 'table': [4045], 'tab': [4049], 'studied': [4060], 'determination': [4062], 'lactones,': [4073, 4159], 'non-cyclic': [4075, 4207, 4219], 'esters.': [4076], '1:6000': [4085], 'relative': [4101], 'activities': [4102], 'AHLs.': [4108, 4132], 'Differences': [4109], 'affect': [4119], 'broad': [4128], 'narrow': [4135, 4295], 'margin,': [4136], 'worked': [4138], 'better': [4139], '3-oxo': [4143, 4346], 'substituted': [4148, 4166], 'derivatives.': [4149, 4323], 'contrast,': [4151], 'members': [4164], 'Noticeably,': [4170], 'γ-decanolactone,': [4176], '6-carbon': [4180], 'alkane': [4181], 'side': [4182, 4318], 'lactonases': [4185], 'digest': [4190], 'p-nitrophenyl': [4191, 4226], 'acetate': [4192, 4230], '(34Hucho': [4193], 'F.': [4194], 'Wallenfels': [4195], 'Biochim.': [4197], 'Biophys.': [4198], 'Acta.': [4199], '1972;': [4200], '276:': [4201], '176-179Google': [4202], 'ester': [4208], 'However,': [4210], 'hydrolyze': [4214], 'ethyl': [4222], 'acetate,': [4223, 4225, 4227], 'phenyl': [4224], 'α-naphthyl': [4229], 'Analysis': [4234], 'AHL-lactonase—Hydrolysis': [4236], 'kinetics': [4237], 'plotting': [4241], 'velocity': [4242], 'versus': [4243], 'concentration.': [4245], 'fitting': [4254], 'equation': [4260], 'II).': [4262], 'comparable': [4265], '20.22–37.63': [4281], 's-1': [4282], '1.43–7.51': [4284], '7.4': [4289], '°C.': [4292], 'ranges,': [4296], '(Km),': [4302], 'slower': [4303], 'rate': [4305], '(kcat),': [4306], 'stronger': [4308], 'efficiency': [4310], '(kcat/Km)': [4311], 'longer': [4316], 'shorter': [4322], 'Additionally,': [4324], 'kcat/Km': [4331], 'fully': [4337], 'reduced': [4338], 'chains': [4340], 'II).Tabled': [4349], '1AHLskcataThe': [4350], 'triplicate': [4355, 4361, 4367], 'experimentsKmaThe': [4356], 'experimentskcat/KmaThe': [4362], 'experimentss-1mmmm-1·s-13-Oxo-C4-HSL28.634.077.033-Oxo-C6-HSL22.682.957.693-Oxo-C8-HSL22.172.289.723-Oxo-C10-HSL20.221.4314.13-Oxo-C12-HSLNDbND': [4368], 'poor': [4374], 'solubility': [4375], 'bufferNDNDC4-HSL37.635.117.36C6-HSL35.673.839.31C8-HSL27.532.6110.5C10-HSLNDbND': [4381]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2062594481', 'counts_by_year': [{'year': 2024, 'cited_by_count': 8}, {'year': 2023, 'cited_by_count': 6}, {'year': 2022, 'cited_by_count': 9}, {'year': 2021, 'cited_by_count': 8}, {'year': 2020, 'cited_by_count': 10}, {'year': 2019, 'cited_by_count': 12}, {'year': 2018, 'cited_by_count': 15}, {'year': 2017, 'cited_by_count': 12}, {'year': 2016, 'cited_by_count': 9}, {'year': 2015, 'cited_by_count': 10}, {'year': 2014, 'cited_by_count': 19}, {'year': 2013, 'cited_by_count': 16}, {'year': 2012, 'cited_by_count': 8}], 'updated_date': '2025-01-06T04:16:15.621827', 'created_date': '2016-06-24'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
