Title: Direct <sup>1</sup>H NMR evidence for conversion of β‐<scp>d</scp>‐cellobiose to cellobionolactone by cellobiose dehydrogenase from <i>Phanerochaete chrysosporium</i>
Abstract: The α‐ and β‐anomers of d ‐cellobiose were resolved by 1 H NMR spectroscopy. Addition of cellobiose dehydrogenase purified from the white‐rot P. chrysosporium led to selective conversion of β‐ d ‐cellobiose. The product was identical to cellobionolactone as synthesized from Ca‐cellobionate. Overnight incubation of the product led to an altered NMR spectrum, which was also obtained by incubation of cellobionolactone. The new spectrum matched that for Ca‐cellobionate. The instability of cellobionolactone explains the detection of cellobionic acid as product in earlier studies.
Publication Year: 1994
Publication Date: 1994-08-29
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 47
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot