Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2062560445', 'doi': 'https://doi.org/10.1016/j.femsle.2005.05.009', 'title': 'A novel β-glucanase gene from<i>Bacillus halodurans</i>C-125', 'display_name': 'A novel β-glucanase gene from<i>Bacillus halodurans</i>C-125', 'publication_year': 2005, 'publication_date': '2005-05-25', 'ids': {'openalex': 'https://openalex.org/W2062560445', 'doi': 'https://doi.org/10.1016/j.femsle.2005.05.009', 'mag': '2062560445', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/15936898'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/j.femsle.2005.05.009', 'pdf_url': 'https://academic.oup.com/femsle/article-pdf/248/1/9/19122771/248-1-9.pdf', 'source': {'id': 'https://openalex.org/S34954451', 'display_name': 'FEMS Microbiology Letters', 'issn_l': '0378-1097', 'issn': ['0378-1097', '1574-6968'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'bronze', 'oa_url': 'https://academic.oup.com/femsle/article-pdf/248/1/9/19122771/248-1-9.pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5110123390', 'display_name': 'M. Akita', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1315852903', 'display_name': 'Japan Agency for Marine-Earth Science and Technology', 'ror': 'https://ror.org/059qg2m13', 'country_code': 'JP', 'type': 'facility', 'lineage': ['https://openalex.org/I1315852903']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Masatake Akita', 'raw_affiliation_strings': ['Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan'], 'affiliations': [{'raw_affiliation_string': 'Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan', 'institution_ids': ['https://openalex.org/I1315852903']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5016178717', 'display_name': 'Kinya Kayatama', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1315852903', 'display_name': 'Japan Agency for Marine-Earth Science and Technology', 'ror': 'https://ror.org/059qg2m13', 'country_code': 'JP', 'type': 'facility', 'lineage': ['https://openalex.org/I1315852903']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Kinya Kayatama', 'raw_affiliation_strings': ['Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan'], 'affiliations': [{'raw_affiliation_string': 'Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan', 'institution_ids': ['https://openalex.org/I1315852903']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5077703123', 'display_name': 'Yuji Hatada', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1315852903', 'display_name': 'Japan Agency for Marine-Earth Science and Technology', 'ror': 'https://ror.org/059qg2m13', 'country_code': 'JP', 'type': 'facility', 'lineage': ['https://openalex.org/I1315852903']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Yuji Hatada', 'raw_affiliation_strings': ['Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan'], 'affiliations': [{'raw_affiliation_string': 'Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan', 'institution_ids': ['https://openalex.org/I1315852903']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5105524415', 'display_name': 'Susumu Ito', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1315852903', 'display_name': 'Japan Agency for Marine-Earth Science and Technology', 'ror': 'https://ror.org/059qg2m13', 'country_code': 'JP', 'type': 'facility', 'lineage': ['https://openalex.org/I1315852903']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Susumu Ito', 'raw_affiliation_strings': ['Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan'], 'affiliations': [{'raw_affiliation_string': 'Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan', 'institution_ids': ['https://openalex.org/I1315852903']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5113548059', 'display_name': 'Koki Horikoshi', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I1315852903', 'display_name': 'Japan Agency for Marine-Earth Science and Technology', 'ror': 'https://ror.org/059qg2m13', 'country_code': 'JP', 'type': 'facility', 'lineage': ['https://openalex.org/I1315852903']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Koki Horikoshi', 'raw_affiliation_strings': ['Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan'], 'affiliations': [{'raw_affiliation_string': 'Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 215 Natsushima, Yokosuka 237 0061, Japan', 'institution_ids': ['https://openalex.org/I1315852903']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2686, 'currency': 'GBP', 'value_usd': 3294, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 2.173, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 45, 'citation_normalized_percentile': {'value': 0.887485, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 92, 'max': 93}, 'biblio': {'volume': '248', 'issue': '1', 'first_page': '9', 'last_page': '15'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11617', 'display_name': 'Enzyme Production and Characterization', 'score': 0.9995, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11617', 'display_name': 'Enzyme Production and Characterization', 'score': 0.9995, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10171', 'display_name': 'Biofuel production and bioconversion', 'score': 0.9983, 'subfield': {'id': 'https://openalex.org/subfields/2204', 'display_name': 'Biomedical Engineering'}, 'field': {'id': 'https://openalex.org/fields/22', 'display_name': 'Engineering'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T12489', 'display_name': 'Microbial Metabolites in Food Biotechnology', 'score': 0.9979, 'subfield': {'id': 'https://openalex.org/subfields/2916', 'display_name': 'Nutrition and Dietetics'}, 'field': {'id': 'https://openalex.org/fields/29', 'display_name': 'Nursing'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/glucanase', 'display_name': 'Glucanase', 'score': 0.8760085}], 'concepts': [{'id': 'https://openalex.org/C2779530776', 'wikidata': 'https://www.wikidata.org/wiki/Q5572295', 'display_name': 'Glucanase', 'level': 3, 'score': 0.8760085}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.536519}, {'id': 'https://openalex.org/C89423630', 'wikidata': 'https://www.wikidata.org/wiki/Q7193', 'display_name': 'Microbiology', 'level': 1, 'score': 0.522945}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.3859644}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.363188}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.35270786}], 'mesh': [{'descriptor_ui': 'D001407', 'descriptor_name': 'Bacillus', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D005798', 'descriptor_name': 'Genes, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D004693', 'descriptor_name': 'Glucan Endo-1,3-beta-D-Glucosidase', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D000595', 'descriptor_name': 'Amino Acid Sequence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001407', 'descriptor_name': 'Bacillus', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': False}, {'descriptor_ui': 'D001407', 'descriptor_name': 'Bacillus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004274', 'descriptor_name': 'DNA, Recombinant', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D004274', 'descriptor_name': 'DNA, Recombinant', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016680', 'descriptor_name': 'Genome, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004693', 'descriptor_name': 'Glucan Endo-1,3-beta-D-Glucosidase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008969', 'descriptor_name': 'Molecular Sequence Data', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/j.femsle.2005.05.009', 'pdf_url': 'https://academic.oup.com/femsle/article-pdf/248/1/9/19122771/248-1-9.pdf', 'source': {'id': 'https://openalex.org/S34954451', 'display_name': 'FEMS Microbiology Letters', 'issn_l': '0378-1097', 'issn': ['0378-1097', '1574-6968'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/15936898', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/j.femsle.2005.05.009', 'pdf_url': 'https://academic.oup.com/femsle/article-pdf/248/1/9/19122771/248-1-9.pdf', 'source': {'id': 'https://openalex.org/S34954451', 'display_name': 'FEMS Microbiology Letters', 'issn_l': '0378-1097', 'issn': ['0378-1097', '1574-6968'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 34, 'referenced_works': ['https://openalex.org/W1534425037', 'https://openalex.org/W1941010610', 'https://openalex.org/W1959423144', 'https://openalex.org/W1971707621', 'https://openalex.org/W1979110812', 'https://openalex.org/W1983109954', 'https://openalex.org/W1998539526', 'https://openalex.org/W2004233096', 'https://openalex.org/W2019913681', 'https://openalex.org/W2020243721', 'https://openalex.org/W2021333226', 'https://openalex.org/W2025661944', 'https://openalex.org/W2027170650', 'https://openalex.org/W2037990513', 'https://openalex.org/W2042017724', 'https://openalex.org/W2054313195', 'https://openalex.org/W2058776624', 'https://openalex.org/W2059297512', 'https://openalex.org/W2059640818', 'https://openalex.org/W2069824228', 'https://openalex.org/W2091576389', 'https://openalex.org/W2094295194', 'https://openalex.org/W2098074194', 'https://openalex.org/W2100837269', 'https://openalex.org/W2115885330', 'https://openalex.org/W2117478535', 'https://openalex.org/W2132417094', 'https://openalex.org/W2137292807', 'https://openalex.org/W2138913088', 'https://openalex.org/W2153407496', 'https://openalex.org/W2156150931', 'https://openalex.org/W2158355883', 'https://openalex.org/W2429757347', 'https://openalex.org/W4249413961'], 'related_works': ['https://openalex.org/W2475095074', 'https://openalex.org/W2371240271', 'https://openalex.org/W2368599855', 'https://openalex.org/W2130076355', 'https://openalex.org/W2119695867', 'https://openalex.org/W2082860237', 'https://openalex.org/W2046158694', 'https://openalex.org/W2034625591', 'https://openalex.org/W1993764875', 'https://openalex.org/W1990804418'], 'abstract_inverted_index': {'A': [0], 'novel': [1, 108, 133], 'endo-beta-1,3(4)-D-glucanase': [2], 'gene': [3, 16, 48, 103, 109, 117, 134, 170, 173, 269], 'was': [4, 17, 58, 75, 110, 174, 212, 220, 229, 254], 'found': [5], 'in': [6, 145, 182, 206], 'the': [7, 34, 39, 45, 71, 81, 87, 102, 116, 120, 132, 149, 153, 166, 169, 172, 202, 217, 226, 243, 266, 268], 'complete': [8], 'genome': [9], 'sequence': [10, 67, 73, 89], 'of': [11, 44, 80, 101, 115, 139, 141, 152, 160, 168], 'Bacillus': [12, 84, 96], 'halodurans': [13, 129, 147], 'C-125.': [14, 130], 'The': [15, 47, 64, 99, 185, 251], 'previously': [18], 'annotated': [19], 'as': [20, 176, 214], 'an': [21, 26, 207, 271], '"unknown"': [22], 'protein': [23, 155, 179], 'and': [24, 41, 53, 125, 144, 162, 180, 195, 223, 225, 239, 248], 'assigned': [25], 'incorrect': [27], 'open': [28], 'reading': [29], 'frame': [30], '(ORF).': [31], 'However,': [32], 'determining': [33], 'biochemical': [35], 'characteristics': [36], 'has': [37], 'elucidated': [38], 'function': [40], 'correct': [42], 'ORF': [43], 'gene.': [46, 98], 'encodes': [49, 270], '231': [50], 'amino': [51, 65], 'acids,': [52], 'its': [54], 'calculated': [55], 'molecular': [56], 'mass': [57], 'estimated': [59], 'to': [60], 'be': [61], '26743.16': [62], 'Da.': [63], 'acid': [66], 'alignment': [68], 'showed': [69, 188, 200], 'that': [70, 79, 105, 114, 159, 201], 'highest': [72], 'identity': [74], 'only': [76], '28%': [77], 'with': [78], 'beta-1,3-1,4-glucanase': [82], 'from': [83, 113, 127, 158], 'subtilis.': [85], 'Moreover,': [86], 'nucleotide': [88], 'did': [90], 'not': [91, 136], 'match': [92], 'any': [93], 'other': [94], 'known': [95], 'beta-glucanase': [97, 122], 'member': [100], 'cluster': [104, 118], 'includes': [106], 'this': [107], 'apparently': [111], 'different': [112], 'including': [119], 'putative': [121, 150], 'genes': [123], '(bh3231': [124], 'bh3232)': [126], 'B.': [128, 146], 'Therefore,': [131], 'is': [135], 'a': [137, 177, 215], 'copy': [138], 'either': [140], 'these': [142], 'genes,': [143], 'cells,': [148], 'role': [151], 'encoded': [154], 'may': [156], 'differ': [157], 'bh3231': [161], 'bh3232.': [163], 'To': [164], 'examine': [165], 'activity': [167, 189, 245, 253], 'product,': [171], 'cloned': [175], 'His-tagged': [178], 'expressed': [181], 'Escherichia': [183], 'coli.': [184], 'purified': [186], 'enzyme': [187, 203], 'against': [190], 'lichenan,': [191], 'barley': [192], 'beta-glucan,': [193], 'laminarin,': [194], 'carboxymethyl': [196], 'curdlan.': [197], 'Thin-layer': [198], 'chromatography': [199], 'hydrolyzes': [204], 'substrates': [205], 'endo-type': [208, 272], 'manner.': [209], 'When': [210], 'beta-glucan': [211], 'used': [213], 'substrate,': [216], 'pH': [218], 'optimum': [219, 228], 'between': [221], '6': [222], '8,': [224], 'temperature': [227], '60': [230, 240], 'degrees': [231, 241, 262], 'C.': [232, 263], 'After': [233], '2': [234], 'h': [235], 'incubation': [236, 259], 'at': [237, 260], '50': [238], 'C,': [242], 'residual': [244], 'remained': [246], '100%': [247], '50%,': [249], 'respectively.': [250], 'enzymatic': [252], 'abolished': [255], 'after': [256], '30': [257], 'min': [258], '70': [261], 'Based': [264], 'on': [265], 'results,': [267], 'beta-1,3(4)-D-glucanase': [273], '(E.C.': [274], '3.2.1.6).': [275]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2062560445', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 6}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2024-12-11T08:17:16.643137', 'created_date': '2016-06-24'}