Title: Concentration-dependent effects of fatty acids on warfarin binding to albumin
Abstract: The interrelationships between fatty acid and warfarin binding to albumin were investigated. Various molar ratios of palmitic acid and oleic acid were added to defatted human albumin in the presence of warfarin, and the warfarin binding association constants, Ka, were calculated. Warfarin association constants increased from 0.84 × 105 M −1 to 3.66 × 105 M −1 as the oleic acid concentration increased from zero to three moles per mole of albumin and from 1.19 × 105 M −1 to 3.13 × 105 M −1 as the palmitic acid concentration increased from zero to two moles per mole of albumin. Larger amounts of either fatty acid progressively decreased the amount of warfarin bound in a noncompetitive fashion. In addition, two proteolytic fragments were utilized to define the location of the warfarin binding site on albumin. The warfarin site was located between loops 5 and 6 on the albumin molecule in close proximity to the secondary and tertiary binding sites of palmitic acid.
Publication Year: 1977
Publication Date: 1977-06-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 49
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