Title: The assay of aromatic amino acid decarboxylase using radioactive substrates
Abstract: The enzyme aromatic amino acid decarboxylase and the enzymatic decarboxylation of DOPA in tissue homogenates and physiological fluids is being extensively studied at present because of the possible involvement of this enzyme in the pathogenesis of Parkinson's disease (1) and the therapeutic use of L-DOPA (2). Determination of the enzymatic decarboxylation of DOPA involves either measurement of dopamine (3–7) or CO2 (8–10). Since the determination of CO2 is relatively simple and rapid, this procedure is now frequently used to estimate enzyme activity, dopamine formation, and/or DOPA disappearance.Recent studies in our laboratory have shown, however, that the measurement of the decarboxylation of DOPA can easily lead to false results.
Publication Year: 1964
Publication Date: 1964-12-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 14
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot