Title: Conversion of raft associated prion protein to the protease-resistant state requires insertion of PrP-res (PrPSc) into contiguous membranes
Abstract: Article1 March 2002free access Conversion of raft associated prion protein to the protease-resistant state requires insertion of PrP-res (PrPSc) into contiguous membranes Gerald S. Baron Gerald S. Baron Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Kathy Wehrly Kathy Wehrly Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author David W. Dorward David W. Dorward Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Bruce Chesebro Bruce Chesebro Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Byron Caughey Corresponding Author Byron Caughey Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Gerald S. Baron Gerald S. Baron Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Kathy Wehrly Kathy Wehrly Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author David W. Dorward David W. Dorward Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Bruce Chesebro Bruce Chesebro Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Byron Caughey Corresponding Author Byron Caughey Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA Search for more papers by this author Author Information Gerald S. Baron1, Kathy Wehrly1, David W. Dorward1, Bruce Chesebro1 and Byron Caughey 1 1Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT, 59840 USA *Corresponding author. E-mail: [email protected] The EMBO Journal (2002)21:1031-1040https://doi.org/10.1093/emboj/21.5.1031 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Prion protein (PrP) is usually attached to membranes by a glycosylphosphatidylinositol-anchor that associates with detergent-resistant membranes (DRMs), or rafts. To model the molecular processes that might occur during the initial infection of cells with exogenous transmissible spongiform encephalopathy (TSE) agents, we examined the effect of membrane association on the conversion of the normal protease-sensitive PrP isoform (PrP-sen) to the protease-resistant isoform (PrP-res). A cell-free conversion reaction approximating physiological conditions was used, which contained purified DRMs as a source of PrP-sen and brain microsomes from scrapie-infected mice as a source of PrP-res. Interestingly, DRM-associated PrP-sen was not converted to PrP-res until the PrP-sen was either released from DRMs by treatment with phosphatidylinositol-specific phospholipase C (PI-PLC), or the combined membrane fractions were treated with the membrane-fusing agent polyethylene glycol (PEG). PEG-assisted conversion was optimal at pH 6–7, and acid pre-treating the DRMs was not sufficient to permit conversion without PI-PLC or PEG, arguing against late endosomes/lysosomes as primary compartments for PrP conversion. These observations raise the possibility that generation of new PrP-res during TSE infection requires (i) removal of PrP-sen from target cells; (ii) an exchange of membranes between cells; or (iii) insertion of incoming PrP-res into the raft domains of recipient cells. Introduction Transmissible spongiform encephalopathies (TSEs) are infectious neurodegenerative diseases naturally occurring in humans (Creutzfeldt–Jakob disease) and a variety of animals including cattle (bovine spongiform encephalopathy), sheep and goats (scrapie). The infectious agent responsible for TSEs, often called the prion, has yet to be fully defined (Chesebro, 1998, 1999). In contrast, some researchers have argued that evidence has accumulated that strongly suggests that the agent is composed of an abnormal isoform of a glycosylphosphatidylinositol (GPI)-anchored protein named PrP (reviewed in Prusiner, 1998). The two isoforms of PrP have been designated PrP-sen (or PrPC) and PrP-res (or PrPSc), based on their respective sensitivity or resistance to protease digestion. TSE agent propagation may occur through the PrP-res-directed conversion of PrP-sen to new PrP-res. This process has been modeled in cell-free reactions (Kocisko et al., 1994; Horiuchi et al., 1999; Saborio et al., 2001). However, no in vitro-generated PrP-res has been shown to be infectious (Hill et al., 1999; Caughey et al., 2001). Although the mechanism by which PrP-sen is converted to PrP-res in TSE-infected cells and in vivo is not clear, data from cell-free reactions suggest this process is akin to autocatalytic polymerization (reviewed in Caughey et al., 2001). Some of our knowledge of the conversion process is derived from studies of neuronal cell lines persistently infected with scrapie (ScN2a cells). Based on this model, the conversion event has been proposed to occur either on the cell surface or at some point during the endocytic trafficking of PrP-sen (Caughey and Raymond, 1991; Caughey et al., 1991; Borchelt et al., 1992). PrP-res then appears to accumulate in lysosomes (Caughey et al., 1991; McKinley et al., 1991). This is consistent with some reports of disease-specific PrP localization in vivo (Laszlo et al., 1992; Arnold et al., 1995; Grigoriev et al., 1999). However, others find that in vivo most disease-specific PrP accumulates extracellularly or on neuronal plasmalemma, and have suggested the possibility that the conversion process may be primarily extracellular (Jeffrey et al., 1992, 1994, 1997). Similarly, a large proportion of PrP-res is also surface localized in ScN2a cells (Vey et al., 1996). Like other GPI-anchored proteins, PrP is enriched in sphingolipid-cholesterol-rich membrane microdomains, or rafts (Vey et al., 1996). These membranes are also referred to as detergent-resistant membranes (DRMs) based on their insolubility in cold non-ionic detergents (e.g. Triton X-100) (Brown and Rose, 1992). Several lines of evidence suggest a role for rafts in the formation of PrP-res in scrapie-infected cultured cells: (i) PrP-sen and PrP-res co-localize to DRM fractions (Vey et al., 1996; Naslavsky et al., 1997); (ii) cholesterol depletion renders PrP-sen soluble in cold Triton X-100 and decreases PrP-res formation in ScN2a cells (Taraboulos et al., 1995); (iii) recombinant transmembrane forms of PrP-sen that are not localized to rafts do not serve as substrates for PrP-res formation in ScN2a cells (Taraboulos et al., 1995; Kaneko et al., 1997); (iv) depletion of cellular sphingolipids increases PrP-res biosynthesis (Naslavsky et al., 1999); and (v) treatment with the sterol-binding anti-scrapie drug amphotericin B modifies the floatation properties of DRMs and inhibits PrP-res synthesis (Mangé et al., 2000). Thus, rafts have attracted attention as a candidate site for the generation of PrP-res in cells. Interactions of PrP-res with its protease-sensitive precursor, PrP-sen, are of central importance in TSE diseases. Extensive structural analyses of PrP-sen, and to a lesser extent PrP-res, have provided valuable information regarding the conformational differences between these two molecules. However, few studies have considered the GPI-anchored nature of PrP and the possible effects of membrane attachment on PrP-sen structure (DeArmond et al., 1999; Morillas et al., 1999; Zuegg and Gready, 2000) and its binding interactions with PrP-res. Although cell-free studies have provided valuable insight into the binding and conversion of PrP-sen by PrP-res, all of these studies used purified PrP-sen, often in a GPI-anchor-deficient form, and most contained denaturant and/or detergent (Kocisko et al., 1994, 1995; Bessen et al., 1997; Horiuchi et al., 1999, 2000; Wong et al., 2001). Here, we have directly assessed the ability of raft-associated PrP-sen to interact with exogenous PrP-res molecules in an attempt to model the molecular processes that might occur during the initial infection of cells with exogenous TSE agents. Interactions between PrP-sen and PrP-res were assayed using a cell-free conversion reaction (Kocisko et al., 1994; Horiuchi et al., 1999) under near-physiological conditions in the absence of denaturants and detergents. Our data suggest that raft-bound PrP-sen is not a substrate for conversion to the protease-resistant state by exogenous PrP-res molecules unless the PrP-res is itself inserted into contiguous membranes. Results PrP-sen is enriched in DRMs The Neuro2a mouse neuroblastoma cell line is widely used for TSE research. However, this cell line also expresses relatively low levels of PrP-sen compared with transfected cell lines. To facilitate our studies, we used Neuro2a cells that overexpress wild-type mouse PrP-sen (Wong et al., 2001). By fluorescence-activated cell-sorting analysis, the new cell line, designated 5E4E, expressed ∼10-fold higher levels of PrP-sen as compared with Neuro2a cells (K.Wehrly and B.Chesebro, unpublished data). Others have shown that in neuroblastoma cells, PrP-sen is localized to low density DRMs (Gorodinsky and Harris, 1995; Vey et al., 1996; Naslavsky et al., 1997). To ensure that PrP-sen remained localized in DRMs, 5E4E cells were solubilized in cold Triton X-100 and subjected to floatation on Optiprep gradients using a protocol we modified from Naslavsky et al. (1997). Gradient fractions were collected and analyzed for the presence of PrP-sen as well as the DRM marker ganglioside GM1 (Naslavsky et al., 1997). As shown in Figure 1A, virtually all of the detectable PrP-sen was localized in the low density fractions of the Optiprep gradient. Estimates of total protein content in the gradient fractions by liquid scintillation counting of fractions from metabolically labeled cells indicated that the DRMs contained ∼3% of the total label incorporated (data not shown), consistent with the levels reported by others (Vey et al., 1996). Ganglioside GM1 was found to co-localize with PrP-sen in these low density fractions (Figure 1C). However, when 5E4E cells were solubilized at 37°C, conditions that solubilize DRM-associated molecules, both PrP-sen and ganglioside GM1 were shifted to higher density fractions (Figure 1B and D). PrP-sen was similarly shifted to higher density fractions when lysed at 4°C with 1% N-octyl β-D-glucopyranoside (data not shown), a raft-disrupting detergent (Brown and Rose, 1992). The data shown in Figure 1 indicate that PrP-sen in 5E4E cells is enriched in DRMs. Figure 1.PrP-sen is enriched in DRMs. Mouse neuroblastoma cells (5E4E) overexpressing wild-type mouse PrP were lysed in CBS (pH 6.0) with 1% Triton X-100 at either 4 or 37°C. Lysates were subjected to floatation on Optiprep gradients. Gradient fractions were collected and examined by immunoblotting for the presence of PrP (A and B) and the DRM marker ganglioside GM1 (C and D). Fraction numbers are indicated above each lane. Molecular mass markers are indicated in kDa on the left. The data are representative of two independent experiments. Download figure Download PowerPoint PrP-sen is surface localized in purified DRMs Since we planned to use DRMs as a means to assay the interaction of PrP-res with DRM-associated PrP-sen, it was important to have a measure of the amount of surface-accessible PrP-sen as purified DRMs are known to contain a large proportion of vesicle-like structures (Brown and Rose, 1992; Sargiacomo et al., 1993; Vey et al., 1996). Purified DRMs were treated with proteinase K (PK) in the absence or presence of 1% Triton X-100 at 37°C and assayed for the presence of protease-resistant PrP by immunoblotting. In the absence of detergent, the amount of PK-resistant protein would be an indicator of the amount of PrP-sen protected from digestion as a consequence of being localized within the lumen of the DRMs. As shown in Figure 2, the vast majority of the PrP-sen in purified DRMs was susceptible to PK digestion. Quantitation of the bands indicated that ∼8% of the total PrP in purified DRMs was PK resistant and thus localized to the lumen of DRMs. This was confirmed by the absence of PK-resistant PrP in parallel reactions containing Triton X-100 (at 37°C), where the DRMs would be disrupted and both luminal and surface-localized PrP-sen would be exposed to PK. Similar results were obtained by labeling purified DRMs with a membrane-impermeable biotinylation reagent (sulfo-NHS-LC-biotin) and measuring the amount of PrP precipitated by treatment with neutravidin–agarose (data not shown). Thus, the above data suggest that ∼92% of the PrP-sen in purified DRMs was surface localized. Figure 2.PrP-sen is surface localized in purified DRMs. DRMs were digested with PK in the presence or absence of detergent (1% Triton X-100) at 37°C. Samples were assayed for PrP by immunoblotting. Results are representative of two independent experiments, each performed in duplicate. Download figure Download PowerPoint Microsomes from scrapie-infected mouse brain contain PrP-res To permit the use of DRMs as a source of PrP-sen in cell-free conversion reactions, a detergent-free fraction of PrP-res was required. The PrP-res used in the reactions was contained in a crude brain membrane fraction (see Materials and methods) from a mouse infected with the mouse-adapted scrapie strain 87V. This fraction contains membrane vesicles that may be derived from all types of cellular membranes including plasma, synaptosomal and endoplasmic reticulum membranes, for example. For convenience, we refer to this fraction as ‘microsomal’. We confirmed that the 87V microsomal fraction contained PrP-res by immunoblotting. As shown in Figure 3, although microsomes from both normal and scrapie-infected mouse brains contained PrP, only microsomes from infected animals (Sc+) contained PrP-res. After PK digestion, the microsome-associated PrP-res closely resembled purified, PK-digested 87V PrP-res (Figure 3), including the ∼6–8 kDa truncation corresponding to the removal of the N-terminal ∼67 amino acids (Prusiner et al., 1984; Oesch et al., 1985; Hope et al., 1986). Figure 3.Immunoblot analysis of PrP in mouse brain microsomes. Crude microsomes were prepared from the brains of normal (Sc−) or 87V scrapie-infected (Sc+) mice and assayed for total PrP (PK−) or PrP-res (PK+) by immunoblotting of untreated or PK-digested samples. Samples of PrP-res purified from 87V-infected mice (87V PrP-res) without (PK−) and with (PK+) PK digestion are shown for comparison. Download figure Download PowerPoint Membrane-associated PrP-sen resists conversion to PrP-res To directly assess whether raft-associated PrP-sen can serve as a substrate for the generation of new PrP-res, we purified DRMs from metabolically labeled 5E4E cells ([35S]DRMs) and used these membranes in cell-free conversion reactions. When [35S]DRMs were mixed with PrP-res-containing microsomes, we were unable to detect the generation of any PrP-res-like PK-resistant bands (Figure 4, lane 5). We observed the same results using PrP-sen contained in microsomes from neuroblastoma cells, in rafts prepared from multiple cell lines and using rafts prepared by detergent-based and detergent-free methods (data not shown). Similarly, purified PrP-sen reconstituted into raft-like liposomes (Schroeder et al., 1994) was not converted to new PrP-res when incubated with PrP-res-containing microsomes (data not shown). Collectively, our data suggest that membrane-associated PrP-sen resists conversion to PrP-res when simply incubated in the presence of exogenous PrP-res molecules. Figure 4.PI-PLC assists cell-free conversion of DRM-associated PrP-sen. Cell-free conversion reactions were performed using [35S]DRMs and crude microsomes from the brains of normal (PrP-res negative lanes) or scrapie-infected (PrP-res positive lanes) mice. PI-PLC was added to the reactions where indicated. PK− lanes contain a one-tenth aliquot of each reaction mixture before PK digestion and are shown at reduced contrast to allow visualization of individual bands. PK+ lanes contain a nine-tenths aliquot of each reaction mixture after PK digestion. Arrows indicate PK-resistant [35S]PrP-res bands (lane 6). The data are representative of several independent experiments. Download figure Download PowerPoint PI-PLC treatment assists cell-free conversion of DRM-associated PrP-sen To determine whether release of PrP-sen from DRMs would permit its conversion to PrP-res, conversion reactions were performed in the presence of phosphatidylinositol-specific phospholipase C (PI-PLC), an enzyme that cleaves off the diacylglycerol moiety of the GPI anchor and releases the remainder of PrP-sen, but not PrP-res, from the surface of intact cells (Stahl et al., 1987; Caughey et al., 1989, 1990). The addition of PI-PLC to conversion reactions with [35S]DRMs produced three protease-resistant bands after PK treatment (Figure 4, lane 6). The apparent molecular mass and pattern of the bands were similar to those of the input PrP-res after PK digestion. DRM conversion reactions conducted using purified 87V PrP-res exhibited the same requirement for PI-PLC digestion, although the conversion efficiency was apparently reduced when compared with reactions with 87V microsomes (data not shown). Due to the reduced conversion efficiency, we did not pursue further DRM conversion studies with purified 87V PrP-res. When PI-PLC was added to reactions with normal brain microsomes, no protease-resistant bands were detected (Figure 4, lane 4), indicating that the protease-resistant bands observed with 87V microsomes were PrP-res dependent. Subsequent experiments discussed below showed that the PK-resistant bands were glycoforms of PrP. Thus, DRM-associated PrP-sen serves as a substrate for PrP-res synthesis after PI-PLC cleavage. The DRM cell-free conversion product is PrP To confirm that the PK-resistant bands observed above corresponded to conversion of PrP-sen to the protease-resistant state, we analyzed the immunoreactivity of the conversion products by immunoprecipitation with PrP-specific antiserum (R30) against a synthetic PrP peptide. The R30 antibody precipitated the three PK-resistant bands from a [35S]DRM conversion reaction with PI-PLC (Figure 5, lane 3) but did not precipitate any proteins from a negative control reaction lacking PrP-res (Figure 5, lane 5). Precipitation of the [35S]DRM bands was competed in the presence of the cognate peptide for the R30 antibody, demonstrating the specificity of the immunoprecipitation. Similar results were obtained from conversion reactions using purified, PI-PLC-digested [35S]PrP-sen (Figure 5, lanes 10–12). Therefore, the PK-resistant bands produced in the [35S]DRM conversion reaction are composed of protease-resistant PrP. Figure 5.The [35S]DRM conversion product is PrP. Conversion reactions were performed as in Figure 4 using microsomes and [35S]DRMs (with PI-PLC; lanes 1–5) or PI-PLC-digested [35S]PrP-sen (lanes 6–12). Conversion reaction products were immunoprecipitated using either R30 antiserum against a mouse PrP synthetic peptide (89–103; lanes 3, 5, 10 and 12) or R30 pre-absorbed with peptide 89–103 (lanes 4 and 11). Conversion reactions without immunoprecipitation (lanes 1, 2 and 6–9) are shown for comparison. PK− and PK+ indicate lanes without and with PK digestion, respectively. PK+ lanes contain nine times the reaction equivalents loaded in PK− lanes, except for the immunoprecipitated samples where the entire reaction was PK digested. Arrows indicate PK-resistant [35S]PrP-res bands (lanes 2, 3, 9 and 10). The data are representative of a single experiment performed in duplicate. Download figure Download PowerPoint PEG treatment assists cell-free conversion of DRM-associated PrP-sen It is possible that conversion of membrane-associated PrP-sen to PrP-res may require insertion of PrP-res seeds into PrP-sen-containing membranes. To test this idea, we attempted to induce fusion of DRMs and microsomes using a protocol commonly employed to generate hybridomas involving a brief treatment with polyethylene glycol (PEG) (Harlow and Lane, 1988). As shown in Figure 6A (lane 4), treatment with a high concentration of PEG (30%) allowed conversion of DRM-bound PrP-sen. In contrast, treatment with an intermediate PEG concentration (15%) allowed at best a very low level of conversion (Figure 6A, lane 3). Based on the input amount of [35S]PrP-sen derived by immunoprecipitation of PrP from [35S]DRMs, we estimated the conversion efficiency of PI-PLC and 30% PEG-treated reactions to be 17 and 25%, respectively. This corresponds to about half the efficiency observed with reactions using purified components under related conditions (Wong et al., 2001). A requirement for 30% PEG or PI-PLC treatment to assist PrP conversion was also observed using rafts from two other Neuro2a cell lines and prepared by detergent-based and detergent-free methods (Smart et al., 1995), suggesting the results are neither cell clone specific nor artifacts of the raft preparation procedure (data not shown). Occasionally, small amounts of two lower molecular mass species (∼21 kDa) were observed in all lanes, likely resulting from incomplete PK digestion. We also verified that PEG pre-treatment of 87V microsomes alone did not enhance their inherent converting activity as measured by conversion of purified PrP-sen (data not shown). Electron microscope (EM) analysis of DRM/normal microsome mixtures incubated with or without PEG has shown an increase in vesicle size only after treatment with 30% PEG, suggesting membrane fusion had occurred (Figure 7). Biosafety considerations prohibited us from EM analysis of scrapie brain microsomes. Although we cannot entirely rule out the possibility that a PEG-dependent effect other than membrane fusion is responsible for assisting conversion of DRM-associated PrP-sen, control reactions using purified PrP-sen suggest that the PEG treatment we have used does not have a significant direct effect on the conversion reaction (data not shown). Together, these data provide evidence that introduction of PrP-res, either alone or along with another microsome component, into DRMs or contiguous membrane domains is critical for conversion of DRM-associated PrP-sen to PrP-res. Figure 6.PEG assists cell-free conversion of DRM-associated PrP-sen. (A) PEG-assisted [35S]DRM conversion reactions. Samples were treated with various concentrations of PEG or PI-PLC as indicated and cell-free conversion was performed as in Figure 4. PrP-sen was immunoprecipitated from one-fifth equivalents of the [35S]DRMs added to the reaction for comparison (DRM RIP, lane 6). The data are representative of several independent experiments, each performed in duplicate. Arrows indicate the PK-resistant [35S]PrP-res bands (lanes 4 and 5). (B) Effect of PNGase F and PI-PLC treatment. Cell-free conversions were performed using [35S]DRMs treated with 30% PEG or PI-PLC (lanes 1–4). Where indicated, PEG-treated [35S]DRM reactions were also digested with PI-PLC after conversion to demonstrate the PEG-assisted conversion product is GPI anchored (lane 3). For comparison, conversion reactions using purified 87V PrP-res and purified PI-PLC-digested or GPI-anchored [35S]PrP-sen are shown (lanes 5–8). All reactions were deglycosylated with PNGase F after conversion. An arrow indicates the [35S]PrP-res bands (lanes 2–6). Download figure Download PowerPoint Figure 7.PEG induces membrane fusion in DRM/microsome mixtures. Mixtures of DRMs and normal brain microsomes were subjected to the designated PEG treatment protocol used in the conversion reactions and analyzed by negative staining EM. Bar = 500 nm. Download figure Download PowerPoint To further characterize the DRM cell-free conversion products, we treated the reactions with PNGase F after conversion to remove N-linked glycans and compared them to products generated in reactions using purified [35S]PrP-sen and PrP-res. The exact co-migration of the PI-PLC-assisted [35S]DRM conversion product with the product from a reaction using purified [35S]PrP-sen lacking a GPI anchor (Figure 6B, compare lanes 4 and 5) suggested that the PI-PLC-assisted converting species from [35S]DRMs lacked a GPI anchor, as GPI-anchored PrP can be distinguished from PI-PLC-cleaved PrP by slightly reduced electrophoretic mobility of the latter on SDS–PAGE (Caughey et al., 1989; Narwa and Harris, 1999). Conversely, the increased electrophoretic mobility of the PEG-assisted DRM conversion products (Figure 6B, lane 2) suggested that these conversion products were GPI anchored. By digestion with PI-PLC after conversion, we confirmed that the PEG-assisted DRM conversion products were indeed GPI anchored (Figure 6B, lanes 2 and 3), consistent with the model that these products were derived from the fusion of DRMs and microsomes. pH dependence of PEG-assisted DRM conversions The nature of the cellular compartment in which PrP conversion occurs has been a subject of debate in the TSE field. Locations from the extracellular milieu through the entire endocytic pathway to lysosomes have all been suggested. We used our DRM conversion system in an attempt to reconstitute these various compartments with respect to containing membrane-associated PrP-sen, PrP-res and a range of pH to identify conditions compatible with conversion in this system. As shown in Figure 8, conversion occurred most efficiently at pH 6–7 and was poor below pH 6.0. Figure 8.pH dependence of PEG-assisted [35S]DRM conversion reactions. Conversion reactions with microsomes and [35S]DRMs and 30% PEG treatment were performed as in Figure 6. Following PEG treatment, the reactions were incubated in buffers of decreasing pH and assayed for PrP-res. PrP-sen was immunoprecipitated from one-fifth equivalents of the [35S]DRMs added to the reaction for comparison (DRM RIP, lane 9). Arrows indicate the PK-resistant [35S]PrP-res bands (lanes 4, 6 and 8). The data are representative of two independent experiments, each performed in duplicate. Download figure Download PowerPoint Acid pre-treatment does not allow cell-free conversion of DRM-associated PrP-sen Recent studies have suggested acidification of PrP may facilitate its conversion to PrP-res (Jackson et al., 1999; Swietnicki et al., 2000). To determine whether an acid pre-treatment might permit conversion of DRM-bound PrP-sen, DRMs were treated with various acidic buffers on ice, washed with citrate buffer (pH 6.0) and used in conversion reactions. As shown in Figure 9, conversion with acid pre-treated DRMs still required PI-PLC, indicating that acid pre-treatment alone was insufficient to allow conversion of DRM-associated PrP-sen. Figure 9.Acid pre-treatment does not facilitate cell-free conversion of DRM-associated PrP-sen. [35S]DRMs were incubated in buffers of decreasing pH, washed in CBS and used in cell-free conversion reactions as in Figure 4. Arrows indicate the PK-resistant [35S]PrP-res bands (lanes 1, 4, 7 and 10). The data are representative of a single experiment performed in duplicate. Download figure Download PowerPoint Discussion In this study, we used DRMs purified from neuroblastoma cells as a source of membrane-bound PrP-sen and tested the ability of this PrP-sen to interact with PrP-res using a cell-free conversion assay in the absence of denaturant and detergent. Since the majority of PrP-sen in cells is associated with DRMs (Figure 1), the PrP-sen used in our experiments is provided in a context approximating that of native PrP-sen with respect to both membrane association as well as other DRM-bound molecules, some of which may influence the conversion process. Thus, these studies serve to bring the cell-free reaction closer to reconstituting the conditions under which the reaction might occur in vivo as cells are initially infected or as TSE agents are transmitted between cells. Using these near-physiological cell-free conversion conditions, we found that simply incubating DRM-associated PrP-sen with PrP-res did not allow conversion to occur. This raises the possibility that DRM-associated PrP-sen may be incapable of binding to exogenous PrP-res, although binding can occur without conversion (Horiuchi et al., 2000, 2001). Since PrP-res is likely a component of TSE infectivity and PrP-res binds to PrP-sen in solution with high specificity (Horiuchi et al., 1999), this interaction is a good candidate for a ligand–receptor binding event that some have suggested may explain the requirement for PrP in TSE diseases (Chesebro, 1998, 1999). However, our data might challenge the idea that PrP-sen could act as a conventional receptor as DRM-bound PrP-sen is not converted to PrP-res without insertion of PrP-res into the same membrane. Previous studi