Title: The Influence of Prolonged Ethanol Intake on the Levels and Turnover of Alcohol and Aldehyde Dehydrogenases and of Brain (Na + K)‐ATPase of Rats
Abstract: Levels of liver alcohol dehydrogenase, liver mitochondrial aldehyde dehydrogenase and brain (Na + K)‐stimulated ATPase were determined in control rats and in rats fed an ethanol liquid diet for 7 weeks. The alcohol dehydrogenase level did not change with the diet; however, the levels of mitochondrial aldehyde dehydrogenase and of the (Na + K)‐ATPase were increased 2.5‐fold and 2‐fold respectively. Also, the levels of these enzymes from brain and liver fractions of rats given a 20% ethanol solution as the sole drinking fluid were determined at intervals from 4 to 18 weeks. A progressive increase was found in the level of aldehyde dehydrogenase and of ATPase. To ascertain whether synthesis or degradation of these enzymes were responsible for the marked increase in activity the turnover of several liver fractions and of the indicated enzymes were measured by the dual‐labelled isotope technique. Long‐term ethanol administration did not affect the rate of turnover of the liver cell fractions except for the mitochondrial fraction. This fraction showed a marked decrease in the rate of degradation. The degradation constant of the liver alcohol dehydrogenase did not change; however, the rate of degradation of liver mitochondrial aldehyde dehydrogenase (low‐ K m ) and of brain (Na + K)‐ATPase was decreased.
Publication Year: 1978
Publication Date: 1978-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 52
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