Title: Liquid Chromatography/Mass Spectrometry with Atmospheric Pressure Chemical Ionization of Free Brassinosteroids
Abstract: Rapid Communications in Mass SpectrometryVolume 10, Issue 8 p. 903-906 Research Article Liquid Chromatography/Mass Spectrometry with Atmospheric Pressure Chemical Ionization of Free Brassinosteroids Keiji Gamoh, Corresponding Author Keiji Gamoh Faculty of Education, Kochi University, Akebono-cho, Kochi 780, JapanFaculty of Education, Kochi University, Akebono-cho, Kochi 780, JapanSearch for more papers by this authorHiroyuki Abe, Hiroyuki Abe Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943, JapanSearch for more papers by this authorKatsuya Shimada, Katsuya Shimada Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943, JapanSearch for more papers by this authorSuguru Takatsuto, Suguru Takatsuto Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943, JapanSearch for more papers by this author Keiji Gamoh, Corresponding Author Keiji Gamoh Faculty of Education, Kochi University, Akebono-cho, Kochi 780, JapanFaculty of Education, Kochi University, Akebono-cho, Kochi 780, JapanSearch for more papers by this authorHiroyuki Abe, Hiroyuki Abe Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943, JapanSearch for more papers by this authorKatsuya Shimada, Katsuya Shimada Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943, JapanSearch for more papers by this authorSuguru Takatsuto, Suguru Takatsuto Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943, JapanSearch for more papers by this author First published: 1996 https://doi.org/10.1002/(SICI)1097-0231(19960610)10:8<903::AID-RCM564>3.0.CO;2-2Citations: 34AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Liquid chromatography/mass spectrometry (LC/MS) with atmospheric pressure chemical ionization has been successfully applied to the simultaneous analysis of free brassinosteroids (polyhydroxylated steroids that serve as plant growth regulators) as their naphthaleneboronates. Of a range of analytical conditions that were tested, optimum results were obtained by the use of reversed-phase high-performance liquid chromatography using a mixture of acetonitrile and water. The positive-ion spectra of naphthaleneboronates of brassinosteroids showed pseudo-molecular ions, [M+H]+, and fragment ions corresponding to the losses of hydroxyl groups of the steroidal nucleus. The [M+H]+ ion was the most abundant for brassinolide and the [M+H−H2O]+ ions were the most abundant for castasterone, teasterone and typhasterol. Full-scan mass spectra were readily obtained from 400 ng of free brassinosteroids, while a limit of detection of 2 ng (signal-to-noise ratio 3) was attained in the selected-ion monitoring mode. The application of LC/MS to the study of brassinosteroids in a plant material is demonstrated by the analysis of seeds of Cannabis sativa L. References 1 V. Marquardt and G. Adam, in W. Ebing (ed.), Chemistry of Plant Protection, Springer, Heidleberg, 1991, pp 103– 139. 2 A. Sakurai and S. Fujioka Plant Growth Regul. 13, 147 (1993). 3 K. Gamoh, K. Omote, N. Okamoto and S. Takatsuto J. Chromatogr. 469, 424 (1989). 4 K. Gamoh and S. Takatsuto Anal. Chim. Acta 222, 201 (1989). 5 K. Gamoh, N. Okamoto, S. Takatsuto and I. Tejima Anal. Chim. Acta 228, 307 (1990). 6 K. Gamoh, S. Takatsuto, M. Watabe, H. Arimoto and H. Sawamoto J. Chromatogr. 515, 227 (1990). 7 K. Gamoh, S. Takatsuto and N. Ikekawa Anal. Chim. Acta 256, 319 (1992). 8 S. Takatsuto, B. Ying, M. Morisaki and N. Ikekawa J. Chromatogr. 239, 233 (1982). 9 S. Takatsuto and N. Ikekawa Chem. Pharm. Bull. 34, 3435 (1986). 10 K. Wada, H. Bando and N. Kawahara J. Chromatogr. 644, 43 (1993). 11 R. P. Evershed, M. C. Prescott, M. Kabbouh and H. H. Rees Rapid Commun. Mass Spectrom. 7, 477 (1993). 12 C. H. L. Shackleton J. Steroid Biochem. Mol. Biol. 45, 127 (1993). 13 O. Curcuruto, D. Franchi, M. Hadman, D. Favretto and P. Traldi Rapid Commun. Mass Spectrom. 7, 673 (1993). 14 Y. Kobayashi, K. Saiki and F. Watanabe Biol. Pharm. Bull. 16, 1175 (1993). 15 R. Lafont, E. D. Morgan and I. D. Wilson J. Chromatogr. 658, 31 (1994). 16 S. Asakawa, H. Abe, Y. Kyokawa, S. Nakamura and M. Natsume Biosci. Biotech. Biochem. 58, 219 (1994). 17 K. Gamoh and C. J. W. Brooks Anal. Sci. 9, 549 (1993). 18 K. Gamoh Bunseki Kagaku (in Japanese) 45, 19 (1996). 19 S. Takatsuto, N. Yazawa, M. Ishiguro, M. Morisaki and N. Ikekawa J. Chem. Soc., Perkin Trans. I, 139 (1994). 20 S. Takatsuto J. Chem. Soc., Perkin Trans. I, 1833 (1986). 21 S. Takatsuto J. Chromatogr. A 658, 3 (1994). 22 S. Takatsuto, in H. G. Culter, T. Yokota and G. Adam (Eds.), Brassinosteroids: Chemistry, Bioactivity and Applications (ACS Symposium Series, No. 474), American Chemical Society, Washington, DC, 1991, pp. 107– 120. 23 H. Suzuki, S. Fujioka, S. Takatsuto, T. Yokota, N. Murofushi and A. Sakurai J. Plant Growth Regul. 13, 21 (1993). 24 H. Suzuki, S. Fujioka, S. Takatsuto, T. Yokota, N. Murofushi and A. Sakurai Biosci. Biotech. Biochem. 59, 168 (1995). 25 S. Takatsuto, H. Abe, T. Yokota, K. Shimada and K. Gamoh J. Jpn. Oil Chem. Soc., to be submitted. Citing Literature Volume10, Issue81996Pages 903-906 ReferencesRelatedInformation
Publication Year: 1996
Publication Date: 1996-06-10
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 36
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