Title: P22. Late endocytic pathway in mouse embryos: Implication in spatiotemporal signaling during gastrulation
Abstract: Late endosomes and lysosomes, the late stages of the endocytic pathway are thought to terminate signal transduction by compartmentalising and degrading the signalling molecules, however, their regulatory roles in embryogenesis are not understood well. We focused on the endocytic membrane dynamics in the mouse perigastrulation embryos. The visceral endoderm, a single cell-layered tissue sustaining embryonic growth through nutrients/gas exchange and participating in the axis determination by orchestrating various signalling cascades, exhibits high activity of endocytosis. The apical vacuoles are large digestive compartments located to the apical cytoplasm, and their limiting membranes were positive for lysosome and late endosome marker proteins. We focused on the membrane dynamics of these subcellular compartments to find that the delivery of endosomes to the apical vacuoles involves unique, microautophagy-like process. Mutant embryos with defects in the membrane transport failed to complete gastrulation. These results exhibited that the late stage of endocytic pathway plays fundamental roles in the early embryogenesis.
Publication Year: 2010
Publication Date: 2010-11-01
Language: en
Type: article
Indexed In: ['crossref']
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