Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2056467018', 'doi': 'https://doi.org/10.1074/mcp.m500331-mcp200', 'title': 'Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins', 'display_name': 'Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins', 'publication_year': 2005, 'publication_date': '2005-12-07', 'ids': {'openalex': 'https://openalex.org/W2056467018', 'doi': 'https://doi.org/10.1074/mcp.m500331-mcp200', 'mag': '2056467018', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/16332733'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/mcp.m500331-mcp200', 'pdf_url': 'http://www.mcponline.org/article/S1535947620303467/pdf', 'source': {'id': 'https://openalex.org/S104550190', 'display_name': 'Molecular & Cellular Proteomics', 'issn_l': '1535-9476', 'issn': ['1535-9476', '1535-9484'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.mcponline.org/article/S1535947620303467/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5108779253', 'display_name': 'Leigh Anderson', 'orcid': None}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Leigh Anderson', 'raw_affiliation_strings': ['The Plasma Proteome Institute, Washington, D. C. 20009-3450'], 'affiliations': [{'raw_affiliation_string': 'The Plasma Proteome Institute, Washington, D. C. 20009-3450', 'institution_ids': []}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5076881039', 'display_name': 'Christie L. Hunter', 'orcid': 'https://orcid.org/0000-0003-2587-1489'}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Christie L. Hunter', 'raw_affiliation_strings': ['Applied Biosystems, Foster City, California, 94404'], 'affiliations': [{'raw_affiliation_string': 'Applied Biosystems, Foster City, California, 94404', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 0, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2800, 'currency': 'USD', 'value_usd': 2800, 'provenance': 'doaj'}, 'apc_paid': {'value': 2800, 'currency': 'USD', 'value_usd': 2800, 'provenance': 'doaj'}, 'fwci': 28.904, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 1225, 'citation_normalized_percentile': {'value': 0.961312, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 99, 'max': 100}, 'biblio': {'volume': '5', 'issue': '4', 'first_page': '573', 'last_page': '588'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10519', 'display_name': 'Advanced Proteomics Techniques and Applications', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10519', 'display_name': 'Advanced Proteomics Techniques and Applications', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10683', 'display_name': 'Mass Spectrometry Techniques and Applications', 'score': 0.9999, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T12867', 'display_name': 'Advanced Biosensing Techniques and Applications', 'score': 0.9969, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/replicate', 'display_name': 'Replicate', 'score': 0.6030254}, {'id': 'https://openalex.org/keywords/quantitative-analysis', 'display_name': 'Quantitative Analysis', 'score': 0.5079854}, {'id': 'https://openalex.org/keywords/sample-preparation', 'display_name': 'Sample Preparation', 'score': 0.44978887}, {'id': 'https://openalex.org/keywords/multiplex', 'display_name': 'Multiplex', 'score': 0.43210834}], 'concepts': [{'id': 'https://openalex.org/C10390740', 'wikidata': 'https://www.wikidata.org/wiki/Q2282401', 'display_name': 'Analyte', 'level': 2, 'score': 0.8343561}, {'id': 'https://openalex.org/C71043704', 'wikidata': 'https://www.wikidata.org/wiki/Q17144692', 'display_name': 'Selected reaction monitoring', 'level': 4, 'score': 0.7877209}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.7801572}, {'id': 'https://openalex.org/C43617362', 'wikidata': 'https://www.wikidata.org/wiki/Q170050', 'display_name': 'Chromatography', 'level': 1, 'score': 0.7402397}, {'id': 'https://openalex.org/C80311884', 'wikidata': 'https://www.wikidata.org/wiki/Q7268939', 'display_name': 'Quantitative proteomics', 'level': 4, 'score': 0.6882614}, {'id': 'https://openalex.org/C2781162219', 'wikidata': 'https://www.wikidata.org/wiki/Q26250693', 'display_name': 'Replicate', 'level': 2, 'score': 0.6030254}, {'id': 'https://openalex.org/C162356407', 'wikidata': 'https://www.wikidata.org/wiki/Q180809', 'display_name': 'Mass spectrometry', 'level': 2, 'score': 0.523408}, {'id': 'https://openalex.org/C95986675', 'wikidata': 'https://www.wikidata.org/wiki/Q185168', 'display_name': 'Quantitative analysis (chemistry)', 'level': 2, 'score': 0.5079854}, {'id': 'https://openalex.org/C2779281246', 'wikidata': 'https://www.wikidata.org/wiki/Q172847', 'display_name': 'Peptide', 'level': 2, 'score': 0.50292534}, {'id': 'https://openalex.org/C2778576202', 'wikidata': 'https://www.wikidata.org/wiki/Q2540164', 'display_name': 'Sample preparation', 'level': 2, 'score': 0.44978887}, {'id': 'https://openalex.org/C2780420688', 'wikidata': 'https://www.wikidata.org/wiki/Q759183', 'display_name': 'Immunoassay', 'level': 3, 'score': 0.4472099}, {'id': 'https://openalex.org/C31827203', 'wikidata': 'https://www.wikidata.org/wiki/Q874296', 'display_name': 'Tandem mass spectrometry', 'level': 3, 'score': 0.44417697}, {'id': 'https://openalex.org/C2781188995', 'wikidata': 'https://www.wikidata.org/wiki/Q6934982', 'display_name': 'Multiplex', 'level': 2, 'score': 0.43210834}, {'id': 'https://openalex.org/C146543888', 'wikidata': 'https://www.wikidata.org/wiki/Q425056', 'display_name': 'Blood proteins', 'level': 2, 'score': 0.41698492}, {'id': 'https://openalex.org/C46111723', 'wikidata': 'https://www.wikidata.org/wiki/Q471857', 'display_name': 'Proteomics', 'level': 3, 'score': 0.34150916}, {'id': 'https://openalex.org/C159654299', 'wikidata': 'https://www.wikidata.org/wiki/Q79460', 'display_name': 'Antibody', 'level': 2, 'score': 0.25667986}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.16874066}, {'id': 'https://openalex.org/C60644358', 'wikidata': 'https://www.wikidata.org/wiki/Q128570', 'display_name': 'Bioinformatics', 'level': 1, 'score': 0.10263485}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.09643489}, {'id': 'https://openalex.org/C105795698', 'wikidata': 'https://www.wikidata.org/wiki/Q12483', 'display_name': 'Statistics', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C33923547', 'wikidata': 'https://www.wikidata.org/wiki/Q395', 'display_name': 'Mathematics', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/mcp.m500331-mcp200', 'pdf_url': 'http://www.mcponline.org/article/S1535947620303467/pdf', 'source': {'id': 'https://openalex.org/S104550190', 'display_name': 'Molecular & Cellular Proteomics', 'issn_l': '1535-9476', 'issn': ['1535-9476', '1535-9484'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/mcp.m500331-mcp200', 'pdf_url': 'http://www.mcponline.org/article/S1535947620303467/pdf', 'source': {'id': 'https://openalex.org/S104550190', 'display_name': 'Molecular & Cellular Proteomics', 'issn_l': '1535-9476', 'issn': ['1535-9476', '1535-9484'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 35, 'referenced_works': ['https://openalex.org/W146803481', 'https://openalex.org/W1892810170', 'https://openalex.org/W1912465182', 'https://openalex.org/W1953681441', 'https://openalex.org/W1982294708', 'https://openalex.org/W1984689115', 'https://openalex.org/W1998986765', 'https://openalex.org/W2001601929', 'https://openalex.org/W2004568153', 'https://openalex.org/W2010079667', 'https://openalex.org/W2025976015', 'https://openalex.org/W2031095666', 'https://openalex.org/W2034001550', 'https://openalex.org/W2035037428', 'https://openalex.org/W2053418558', 'https://openalex.org/W2053936196', 'https://openalex.org/W2062203307', 'https://openalex.org/W2068481474', 'https://openalex.org/W2087746647', 'https://openalex.org/W2094539668', 'https://openalex.org/W2101034885', 'https://openalex.org/W2105192683', 'https://openalex.org/W2106583190', 'https://openalex.org/W2133258252', 'https://openalex.org/W2136468934', 'https://openalex.org/W2136851624', 'https://openalex.org/W2137682839', 'https://openalex.org/W2139927267', 'https://openalex.org/W2148799804', 'https://openalex.org/W2149282020', 'https://openalex.org/W2170827690', 'https://openalex.org/W2172116519', 'https://openalex.org/W2220693209', 'https://openalex.org/W2512493356', 'https://openalex.org/W38556188'], 'related_works': ['https://openalex.org/W2774960599', 'https://openalex.org/W2381414844', 'https://openalex.org/W2348302859', 'https://openalex.org/W2283618231', 'https://openalex.org/W2226448988', 'https://openalex.org/W2211110286', 'https://openalex.org/W2119600030', 'https://openalex.org/W2089941226', 'https://openalex.org/W2031488930', 'https://openalex.org/W1969165124'], 'abstract_inverted_index': {'Quantitative': [0, 207, 1010], 'LC-MS/MS': [1, 208, 1706, 2057, 2091, 2163, 2607, 3581, 4253], 'assays': [2, 46, 209, 253, 562, 765, 822, 890, 1101, 1232, 1740, 1767, 2009, 2068, 2137], 'were': [3, 210, 2264, 2581, 2618, 2650, 2699, 2876, 2898, 3041, 3052, 3074, 3093, 3145, 3282, 3306, 3314, 3577, 3636, 3659, 3673, 3776, 3816, 3844, 3867, 3884, 3952, 4046, 4069, 4249, 4311, 4338], 'designed': [4, 211, 3777], 'for': [5, 119, 180, 212, 326, 387, 564, 698, 733, 751, 920, 979, 1167, 1200, 1254, 1294, 1305, 1331, 1361, 2006, 2127, 2138, 2225, 2247, 2260, 2338, 2447, 2460, 2528, 2583, 2645, 2674, 2715, 2776, 2790, 2820, 2880, 3033, 3162, 3177, 3198, 3250, 3698, 3891, 3954, 4015, 4125, 4147, 4235], 'tryptic': [6, 213, 1402, 1453, 2258, 2344, 2525, 2792, 3220, 3253, 3500, 3834, 4159, 4268], 'peptides': [7, 53, 197, 214, 260, 404, 419, 1396, 1838, 1859, 1935, 2028, 2071, 2176, 2259, 2345, 2526, 2535, 2556, 2570, 2589, 2635, 2694, 2793, 2823, 2875, 2891, 2968, 3208, 3281, 3416, 3576, 3697, 3752, 3815, 3843, 3866, 3883, 3897, 3941, 4068, 4160, 4269, 4327, 4347], 'representing': [8, 215, 4328], '53': [9, 216, 4329], 'high': [10, 81, 217, 288, 1091, 1988, 2055, 2076, 2741, 4169, 4200], 'and': [11, 104, 113, 176, 201, 218, 311, 320, 383, 408, 418, 422, 432, 450, 548, 579, 615, 625, 674, 760, 803, 883, 915, 972, 1021, 1025, 1043, 1072, 1164, 1177, 1203, 1263, 1343, 1368, 1436, 1470, 1496, 1540, 1544, 1585, 1684, 1710, 1748, 1752, 1774, 1819, 1860, 1866, 1936, 1942, 1958, 1989, 1999, 2033, 2065, 2093, 2114, 2122, 2167, 2197, 2239, 2271, 2313, 2490, 2493, 2514, 2561, 2641, 2677, 2815, 2847, 2888, 2922, 2926, 2946, 2969, 3021, 3067, 3070, 3147, 3155, 3175, 3196, 3228, 3243, 3293, 3402, 3478, 3484, 3510, 3572, 3606, 3656, 3682, 3753, 3759, 3783, 3813, 3935, 3969, 4020, 4076, 4313], 'medium': [12, 219, 1990, 2078], 'abundance': [13, 82, 190, 220, 289, 397, 495, 1991, 2079, 2140, 2254, 3091, 3996], 'proteins': [14, 83, 138, 191, 221, 290, 345, 398, 417, 496, 643, 1027, 1495, 1660, 1771, 1861, 1937, 1993, 2021, 2081, 2121, 2196, 2244, 2576, 2697, 3092, 3151, 3446, 3714, 3754, 3993, 4127, 4154, 4274, 4292, 4330, 4337], 'in': [15, 56, 60, 75, 98, 147, 162, 169, 222, 263, 267, 282, 305, 354, 369, 376, 420, 476, 491, 660, 711, 730, 799, 817, 847, 865, 877, 884, 891, 948, 985, 1023, 1069, 1181, 1204, 1233, 1260, 1290, 1302, 1324, 1371, 1397, 1422, 1571, 1661, 1703, 1728, 1815, 2029, 2108, 2131, 2154, 2189, 2222, 2317, 2346, 2450, 2539, 2578, 2612, 2808, 2824, 2838, 2870, 2988, 3038, 3152, 3183, 3189, 3213, 3233, 3261, 3288, 3308, 3318, 3408, 3417, 3441, 3520, 3565, 3664, 3678, 3905, 3915, 3983, 3995, 4054, 4073, 4109, 4130, 4161, 4252, 4261, 4331, 4351, 4358], 'human': [16, 223, 445, 520, 986, 1261, 1641, 1662, 1667, 2413, 3081, 3512, 3853, 3932, 4332], 'plasma': [17, 148, 170, 224, 355, 377, 421, 446, 521, 755, 1264, 1405, 1663, 1729, 1977, 1992, 2020, 2031, 2080, 2120, 2210, 2253, 2610, 2798, 3082, 3096, 3133, 3143, 3259, 3320, 3513, 3532, 3539, 3542, 3545, 3547, 3549, 3720, 3854, 3879, 3933, 3992, 4003, 4258, 4333], 'using': [18, 124, 225, 331, 668, 988, 1087, 1265, 1430, 1579, 1666, 1707, 1732, 1862, 1938, 2053, 2149, 2622, 2892, 3097, 3123, 3299, 3443, 3582, 3638, 3661, 3755, 3784, 3921, 3965, 3975, 4099], 'a': [19, 154, 163, 174, 226, 361, 370, 381, 425, 523, 688, 705, 731, 777, 800, 912, 975, 1064, 1112, 1136, 1141, 1149, 1161, 1179, 1182, 1306, 1370, 1451, 1459, 1473, 1520, 1671, 1892, 1909, 2030, 2073, 2125, 2144, 2178, 2192, 2240, 2272, 2387, 2412, 2480, 2558, 2564, 2646, 2774, 2795, 2835, 2839, 2974, 2989, 3079, 3085, 3124, 3223, 3246, 3406, 3566, 3643, 3701, 3716, 3803, 3819, 3833, 3849, 3899, 3925, 3931, 3946, 3970, 4055, 4090, 4110, 4116, 4136, 4140, 4150, 4162, 4233, 4276, 4314, 4323], 'multiplexed': [20, 227, 830, 1225], 'multiple': [21, 228, 594, 621, 628, 669, 727, 889, 1032, 1431, 1580, 3099, 4168], 'reaction': [22, 229, 595, 622, 670, 1033, 1151, 1432, 1581, 2732, 2771, 4191, 4230], 'monitoring': [23, 230, 671, 1152, 1433, 1582, 2733, 2772, 4192, 4231], '(MRM)': [24, 231], 'approach.': [25, 232, 1734], 'Of': [26, 233, 3302, 4290], 'these,': [27, 234], '47': [28, 235], 'produced': [29, 236, 3239, 4295], 'acceptable': [30, 237, 2826], 'quantitative': [31, 238, 552, 565], 'data,': [32, 239, 2166], 'demonstrating': [33, 71, 240, 278], 'within-run': [34, 241], 'coefficients': [35, 109, 242, 316], 'of': [36, 42, 45, 52, 79, 111, 130, 157, 160, 194, 243, 249, 252, 259, 286, 318, 337, 364, 367, 401, 416, 429, 529, 567, 588, 637, 657, 663, 690, 726, 753, 757, 770, 807, 820, 880, 888, 907, 925, 974, 982, 1012, 1015, 1052, 1063, 1067, 1109, 1119, 1128, 1144, 1187, 1218, 1257, 1298, 1308, 1311, 1364, 1394, 1404, 1419, 1425, 1462, 1494, 1522, 1533, 1536, 1568, 1574, 1624, 1658, 1697, 1738, 1754, 1770, 1805, 1812, 1821, 1837, 1858, 1908, 1917, 1934, 1964, 1976, 2075, 2096, 2103, 2106, 2118, 2129, 2146, 2171, 2173, 2177, 2194, 2212, 2215, 2227, 2242, 2251, 2262, 2274, 2291, 2340, 2343, 2378, 2381, 2444, 2458, 2473, 2551, 2573, 2594, 2609, 2627, 2661, 2709, 2737, 2797, 2862, 2928, 2932, 2949, 2958, 2992, 3005, 3078, 3194, 3206, 3222, 3245, 3264, 3271, 3327, 3336, 3376, 3413, 3447, 3465, 3494, 3502, 3525, 3587, 3610, 3695, 3704, 3712, 3719, 3751, 3793, 3826, 3836, 3851, 3862, 3877, 3930, 3978, 4005, 4041, 4066, 4085, 4143, 4149, 4152, 4173, 4196, 4255, 4270, 4316, 4325, 4362], 'variation': [37, 244], '(CVs)': [38, 245], '(n': [39, 246], '=': [40, 247, 3807], '10)': [41, 248], '2–22%': [43, 250], '(78%': [44, 251], 'had': [47, 254, 2557, 2563, 3016], 'CV': [48, 255], '<10%).': [49, 256], 'A': [50, 257, 1251, 3204, 3499, 3617], 'number': [51, 258, 2443, 2457], 'gave': [54, 261, 2483], 'CVs': [55, 88, 262, 295], 'the': [57, 72, 141, 264, 279, 348, 430, 576, 635, 661, 768, 805, 878, 885, 904, 923, 980, 1061, 1117, 1120, 1129, 1145, 1168, 1188, 1201, 1255, 1291, 1295, 1384, 1392, 1423, 1463, 1534, 1537, 1549, 1572, 1698, 1736, 1750, 1755, 1816, 1884, 1888, 1906, 1966, 1971, 1980, 2041, 2097, 2104, 2174, 2223, 2379, 2442, 2448, 2451, 2456, 2461, 2467, 2529, 2548, 2600, 2614, 2623, 2628, 2637, 2656, 2710, 2803, 2822, 2863, 2884, 2905, 2916, 2947, 2950, 2956, 3001, 3006, 3011, 3024, 3098, 3110, 3184, 3219, 3262, 3276, 3309, 3328, 3332, 3340, 3347, 3351, 3355, 3360, 3364, 3368, 3380, 3385, 3389, 3393, 3410, 3414, 3420, 3462, 3503, 3516, 3627, 3639, 3657, 3665, 3680, 3693, 3791, 3794, 3814, 3837, 3858, 3863, 3871, 3878, 3887, 3914, 3937, 3955, 3963, 3979, 3984, 4027, 4051, 4067, 4074, 4131, 4256, 4266, 4308, 4350, 4359], 'range': [58, 156, 265, 363, 435, 1747, 1975, 2550, 3718, 4165], '2–7%': [59, 266], 'five': [61, 268], 'experiments': [62, 269, 3073, 3215], '(10': [63, 270], 'replicate': [64, 271], 'runs': [65, 106, 272, 313, 4254], 'each)': [66, 273], 'continuously': [67, 274], 'measuring': [68, 275], '137': [69, 276], 'MRMs,': [70, 277, 3873], 'precision': [73, 280, 580, 1095], 'achievable': [74, 281], 'complex': [76, 283, 1398], 'digests.': [77, 284], 'Depletion': [78, 285], 'six': [80, 287, 3089], 'by': [84, 192, 203, 291, 399, 410, 527, 617, 1028, 1106, 1131, 1501, 1664, 1705, 1743, 1749, 1772, 1839, 1868, 1912, 1944, 2025, 2090, 2187, 2357, 2391, 2418, 2440, 2455, 2854, 3043, 3149, 3216, 3240, 3426, 3579, 3761, 3802, 3846, 3870, 3907, 4078, 4155, 4340, 4349, 4355], 'immunosubtraction': [85, 292], 'significantly': [86, 293, 572], 'improved': [87, 294, 2336, 4039], 'compared': [89, 296, 3274], 'with': [90, 149, 297, 356, 665, 704, 1055, 1093, 1133, 1206, 1427, 1576, 1629, 1768, 1823, 1959, 2092, 2267, 2422, 2740, 2878, 2938, 2995, 3173, 3218, 3275, 3321, 3419, 3472, 3571, 3598, 3608, 3613, 3798, 3830, 3942, 4034, 4167, 4199, 4282], 'whole': [91, 298, 1372, 3509, 3852], 'plasma,': [92, 299], 'but': [93, 300, 3874], 'analytes': [94, 301, 931], 'could': [95, 302, 2036, 2048, 2086, 2111], 'be': [96, 145, 186, 303, 352, 393, 489, 1173, 1456, 2004, 2023, 2046, 2050, 2087, 2209, 3018, 4104], 'detected': [97, 304, 2464, 2701, 3037, 3317, 3845, 3869, 3885, 3982, 4070, 4129], 'both': [99, 306, 3679], 'sample': [100, 151, 307, 358, 713, 3083, 3114, 3166, 4053], 'types.': [101, 308], 'Replicate': [102, 309], 'digest': [103, 310, 3221, 3501, 3552, 3555, 3558, 3561, 3835, 3850, 3934], 'depletion/digest': [105, 312], 'yielded': [107, 314], 'correlation': [108, 315], '(R2)': [110, 317], '0.995': [112, 319], '0.989,': [114, 321], 'respectively.': [115, 322], 'Absolute': [116, 323, 1492, 1695], 'analyte': [117, 324, 914, 1122, 1170, 1219], 'specificity': [118, 325, 769, 790, 1199, 2869], 'each': [120, 327, 2261, 2848, 3014, 3337, 3711, 3827, 4148, 4174], 'peptide': [121, 328, 676, 1438, 1454, 1481, 1539, 1587, 1910, 2282, 2355, 2449, 2465, 2474, 2845, 2868, 3254, 3338, 3703, 3781, 3828, 3927, 3980, 4016, 4036, 4096], 'was': [122, 329, 2200, 2245, 2255, 2384, 2476, 3115, 3134, 3167, 3187, 3209, 3238, 3470, 3506, 3623, 3708, 3839, 3855, 3973, 4093, 4123, 4145, 4319], 'demonstrated': [123, 330, 2204, 3943], 'MRM-triggered': [125, 332], 'MS/MS': [126, 333, 2107, 2130, 2621, 2857, 3045, 3950, 4010, 4059, 4283], 'scans.': [127, 334], 'Reliable': [128, 335], 'detection': [129, 336, 566, 624, 773, 2383, 4097], 'L-selectin': [131, 338], '(measured': [132, 339], 'at': [133, 340, 634, 1090, 1913, 3159, 3180, 3201, 3331, 3515, 3630, 3675, 4061, 4275, 4296], '0.67': [134, 341], 'μg/ml)': [135, 342], 'indicates': [136, 343], 'that': [137, 184, 344, 391, 436, 1171, 1895, 1987, 2001, 2202, 2482, 2698, 3013, 3342, 3345, 3366, 4107, 4263, 4321], 'down': [139, 346, 3725, 3999], 'to': [140, 188, 347, 395, 474, 482, 488, 708, 716, 775, 910, 1060, 1175, 1226, 1328, 1391, 1518, 1547, 1808, 1904, 1969, 1979, 2045, 2077, 2082, 2184, 2208, 2353, 2479, 2486, 2496, 2543, 2620, 2692, 2734, 2788, 2842, 2858, 2900, 3017, 3109, 3170, 3211, 3359, 3486, 3625, 3685, 3692, 3726, 3857, 3911, 3918, 3940, 4000, 4049, 4103, 4193], 'μg/ml': [142, 349], 'level': [143, 350], 'can': [144, 185, 351, 392, 570, 787, 1172, 1195, 1214, 1455, 2022, 3902], 'quantitated': [146, 353, 1471], 'minimal': [150, 357], 'preparation,': [152, 359], 'yielding': [153, 360, 3490], 'dynamic': [155, 362, 434, 1746, 1974], '4.5': [158, 365], 'orders': [159, 366, 1916], 'magnitude': [161, 368, 1918], 'single': [164, 371, 3080, 3702, 3824, 3900, 4091], 'experiment.': [165, 372, 4134], 'Peptide': [166, 373], 'MRM': [167, 374, 1385, 1766, 1967, 2008, 2067, 2169, 2850, 3290, 3671, 3699, 3919, 4063, 4087, 4100, 4120, 4300, 4305], 'measurements': [168, 375, 919, 1222, 2038], 'digests': [171, 378, 1403, 2611, 3514, 3570], 'thus': [172, 379, 2000, 2954, 3295], 'provide': [173, 380, 571, 717, 788, 1196, 1215, 1227, 2124, 3405], 'rapid': [175, 382], 'specific': [177, 195, 384, 402, 561, 764, 778, 913, 1142, 1163, 1231, 1395, 1452, 1625, 2007, 2844, 4028], 'assay': [178, 385, 900, 1386, 1529, 1911], 'platform': [179, 386], 'biomarker': [181, 388, 701, 709, 1700], 'validation,': [182, 389], 'one': [183, 390, 892, 1234, 3190, 3375, 4271, 4298], 'extended': [187, 394], 'lower': [189, 396], 'enrichment': [193, 400, 1769, 1836], 'target': [196, 403, 2575, 2696, 3396, 4153], '(stable': [198, 405], 'isotope': [199, 406, 613, 1711, 1864, 1940, 3757, 3892], 'standards': [200, 407, 614, 1211, 1865, 1941, 3758], 'capture': [202, 409, 616, 771, 1841, 1867, 1943, 3760], 'anti-peptide': [204, 411, 618, 1869, 1945, 2134, 3762], 'antibodies': [205, 412, 786, 795, 882, 1870, 1946, 3763], '(SISCAPA)).': [206, 413], 'Accurate': [414], 'quantitation': [415, 1217, 1857, 1933, 3662, 3750], 'serum': [423, 662, 987, 1262, 1424, 1573, 1704, 1731, 1820, 2415], 'is': [424, 480, 814, 1468, 1741, 1984, 2806, 2852, 4101], 'challenging': [426], 'problem': [427], 'because': [428], 'complexity': [431], 'extreme': [433, 789, 3333], 'characterize': [437], 'these': [438, 691, 1739, 2037, 2061, 2263, 2574, 3039, 3303, 4044, 4086], 'samples': [439, 3144, 3212], '(1Anderson': [440], 'N.L.': [441, 498, 723, 1844, 1920, 2219, 3737], 'Anderson': [442, 1845, 1921, 3227, 3738], 'N.G.': [443, 1846, 1922, 3739], 'The': [444, 468, 519, 554, 724, 1156, 2523, 2633, 3049, 3088, 3165, 3279, 3325, 3987], 'proteome:': [447, 522, 2416], 'history,': [448], 'character,': [449], 'diagnostic': [451], 'prospects.Mol.': [452], 'Cell.': [453, 533, 736, 850, 2361, 2425, 2743, 4202], 'Proteomics.': [454, 534, 737, 851, 2362, 2426, 2744, 4203], '2002;': [455, 835, 1276, 1375, 1676, 2427], '1:': [456, 1677, 2428], '845-867Abstract': [457], 'Full': [458, 460, 538, 540, 741, 743, 855, 857, 2366, 2368, 2430, 2432, 2748, 2750, 4207, 4209], 'Text': [459, 461, 539, 541, 742, 744, 856, 858, 2367, 2369, 2431, 2433, 2749, 2751, 4208, 4210], 'PDF': [462, 542, 745, 859, 2370, 2434, 2752, 4211], 'PubMed': [463, 543, 681, 746, 838, 860, 957, 998, 1040, 1079, 1279, 1340, 1378, 1443, 1513, 1592, 1636, 1679, 1720, 1830, 1877, 1953, 2234, 2308, 2371, 2435, 2687, 2753, 3455, 3770, 4212], 'Scopus': [464, 544, 682, 747, 839, 861, 958, 999, 1080, 1280, 1379, 1444, 1514, 1593, 1637, 1680, 1721, 1831, 1878, 1954, 2235, 2309, 2372, 2436, 2688, 2754, 3456, 3771, 4213], '(3564)': [465], 'Google': [466, 546, 684, 749, 841, 863, 960, 1001, 1041, 1082, 1282, 1341, 1381, 1446, 1516, 1595, 1639, 1682, 1723, 1833, 1880, 1956, 2237, 2311, 2374, 2438, 2690, 2756, 2786, 3458, 3773, 4215, 4245], 'Scholar).': [467, 685, 1283, 1447, 2375, 3459, 3774], 'widely': [469, 3904], 'adopted': [470], 'separative': [471], '(survey)': [472], 'approach': [473, 707, 1089, 1194, 1387, 1886, 3691, 3917, 4353], 'proteomics,': [475], 'which': [477, 558, 1466, 2248, 2613], 'an': [478, 718, 898, 1528, 1545, 2249, 2707, 2825, 2929, 3468, 3491, 3689, 4356], 'attempt': [479, 2183], 'made': [481], 'detect': [483, 1176, 2843], 'all': [484, 2172, 2821, 3343, 3363, 3841, 4157, 4265], 'components,': [485], 'has': [486, 1388, 1896, 1901, 3339], 'proven': [487], 'limited': [490, 551, 1742], 'sensitivity': [492, 574, 1737, 1907, 4098], 'toward': [493, 641], 'low': [494, 2139], '(2Anderson': [497], 'Polanski': [499], 'M.': [500, 1050, 1353, 2763, 4222], 'Pieper': [501], 'R.': [502, 516, 936, 1318, 1649, 1799, 2324, 2666], 'Gatlin': [503], 'T.': [504, 938, 940, 2767, 4226], 'Tirumalai': [505], 'R.S.': [506], 'Conrads': [507], 'T.P.': [508, 2287], 'Veenstra': [509], 'T.D.': [510], 'Adkins': [511, 2392], 'J.N.': [512, 2396], 'Pounds': [513, 2409], 'J.G.': [514, 2410], 'Fagan': [515], 'Lobley': [517], 'A.': [518, 1046, 1075, 1509, 2304, 2726, 4185], 'non-redundant': [524], 'list': [525], 'developed': [526, 1764, 2143], 'combination': [528, 689, 1205], 'four': [530], 'separate': [531, 2445], 'sources.Mol.': [532], '2004;': [535, 678, 995, 1076, 1440, 1589, 1717, 1827, 1874, 1950, 2363, 2684, 3767], '3:': [536, 1718, 1875, 1951, 2364, 2685, 3768], '311-326Abstract': [537], '(760)': [545], 'Scholar)': [547, 750, 864, 1724, 1834, 1957, 2439, 2691], 'typically': [549], 'provides': [550, 897], 'precision.': [553], 'alternative': [555, 899], 'candidate-based': [556, 706], 'approach,': [557, 901, 2802, 4115], 'relies': [559], 'on': [560, 767, 903, 916, 2070, 2591, 3076, 3285, 3642, 3732, 4286], 'optimized': [563, 3298, 4317], 'selected': [568, 1150, 1169, 1457, 1538, 2246, 2582, 2588, 2874, 3214, 3283, 3786, 4348], 'proteins,': [569], 'increased': [573], '(into': [575], 'pg/ml': [577, 1981], 'range)': [578], '(CVs': [581], '1The': [582], 'abbreviations': [583], 'used': [584, 797, 1174, 2655, 2706, 2833, 2899, 2909, 3307, 3624, 4030, 4048], 'are:': [585], 'CV,': [586], 'coefficient': [587], 'variation;': [589], 'QqQ,': [590], 'triple': [591, 3648], 'quadrupole;': [592], 'MRM,': [593], 'monitoring;': [596], 'S/N,': [597], 'signal-to-noise;': [598], 'Nat,': [599], 'natural': [600, 3277, 3394], 'sample-derived': [601], 'peptide;': [602], 'SIS,': [603], 'stable': [604, 612, 1208, 1479, 1550, 1863, 1939, 3756], 'isotope-labeled': [605, 1209, 1480, 1551], 'internal': [606, 1210, 1475], 'standard;': [607], 'polySIS,': [608], 'polyprotein': [609], 'SIS;': [610], 'SISCAPA,': [611], 'antibodies;': [619], 'MIDAS,': [620], 'monitoring-initiated': [623], 'sequencing;': [626], 'MARS,': [627], 'affinity': [629, 3100], 'removal': [630, 3101], 'system.': [631], '<': [632, 1097], '5–10%)': [633], 'cost': [636], 'restricting': [638], 'discovery': [639, 702], 'potential': [640, 2207], 'novel': [642, 4137], '(3Kuhn': [644, 1406, 1555], 'E.': [645, 1407, 1556, 1783, 2765, 4224], 'Wu': [646, 1408, 1557, 1780], 'J.': [647, 649, 1335, 1409, 1411, 1485, 1558, 1560, 1781, 1795], 'Karl': [648, 1410, 1559, 1794], 'Liao': [650, 1412, 1561], 'H.': [651, 1413, 1562, 1647, 1779], 'Zolg': [652, 1414, 1563, 1800], 'W.': [653, 1314, 1415, 1564, 1785, 1801, 2328], 'Guild': [654, 1416, 1565, 1802], 'B.': [655, 1270, 1316, 1320, 1417, 1566, 1787], 'Quantification': [656, 1418, 1567], 'C-reactive': [658, 1420, 1553, 1569], 'protein': [659, 811, 1003, 1013, 1421, 1464, 1523, 1554, 1570, 1708, 1810, 2198, 2292, 2354, 2531, 2649, 2679, 2738, 2778, 2804, 2872, 3237, 3505, 3822, 3901, 4197, 4237], 'patients': [664, 1426, 1575, 1822], 'rheumatoid': [666, 1428, 1577, 1824], 'arthritis': [667, 1429, 1578], 'mass': [672, 908, 992, 1030, 1058, 1110, 1118, 1158, 1267, 1326, 1357, 1434, 1583, 1713, 1744, 1806, 1889, 2423, 2515, 3027, 3269, 3356, 4170], 'spectrometry': [673, 896, 947, 1031, 1059, 1327, 1358, 1435, 1584, 1807], '13C-labeled': [675, 1437, 1586], 'standards.Proteomics.': [677, 1439, 1588], '4:': [679, 739, 853, 1441, 1590, 2746, 4205], '1175-1186Crossref': [680, 1442, 1591], '(370)': [683, 1445, 1594], 'In': [686, 922, 1190, 1448, 1525, 1882, 2180, 2567, 2599, 2652, 2800, 2904, 2973, 3688, 4083], 'practice,': [687], 'approaches': [692, 697, 2897, 3292, 4310], '(one': [693], 'or': [694, 772, 1229, 1730, 1835, 2205, 2511, 2519, 3141, 3591, 3958, 4272], 'more': [695, 1230, 2132, 3377], 'survey': [696, 2165, 2597, 2836, 3928, 4114, 4133], 'de': [699, 4118], 'novo': [700, 4119], 'coupled': [703, 2421], 'validation': [710], 'large': [712, 2990, 4141], 'sets)': [714], 'appears': [715, 1962], 'effective': [719], 'staged': [720], 'pipeline': [721, 732], '(4Anderson': [722], 'roles': [725], 'proteomics': [728, 1657, 2221, 3442], 'platforms': [729], 'new': [734, 1065, 3567], 'diagnostics.Mol.': [735], '2005;': [738, 852, 2231, 2745, 2783, 3452, 4204, 4242], '1441-1444Abstract': [740], '(111)': [748], 'generation': [752, 1066], 'valid': [754], 'biomarkers': [756, 1014, 1811, 1998, 2226], 'disease,': [758], 'risk,': [759], 'therapeutic': [761], 'response.': [762], 'Candidate-based': [763, 2220], 'rely': [766], 'methods': [774, 1285, 1760], 'select': [776, 911, 2693], 'molecule': [779, 930], 'as': [780, 785, 798, 1401, 1458, 1670, 1891, 1996, 2773, 2834, 3388, 3787, 3818, 4071, 4081, 4232], 'analyte.': [781], 'Capture': [782], 'reagents': [783], 'such': [784, 821, 1100, 1400, 1527], '(particularly': [791], 'when': [792], 'two': [793, 1107, 1157, 2592, 4273, 4341], 'different': [794, 3896], 'are': [796, 1084, 1286, 2203, 2813, 2818, 2831, 2963, 3294], 'sandwich': [801], 'immunoassay)': [802], 'form': [804], 'basis': [806], 'most': [808, 2098, 2462, 4021, 4065, 4084], 'existing': [809, 2054], 'clinical': [810, 1292, 1997], 'assays.': [812], 'There': [813, 1983], 'intense': [815], 'interest': [816], 'miniaturizing': [818], 'sets': [819, 3527], '(5Joos': [823], 'T.O.': [824], 'Stoll': [825], 'D.': [826], 'Templin': [827], 'M.F.': [828], 'Miniaturised': [829], 'immunoassays.Curr.': [831], 'Opin.': [832], 'Chem.': [833, 994, 1374, 1632], 'Biol.': [834], '6:': [836], '76-80Crossref': [837], '(94)': [840], 'Scholar,': [842, 2757, 4216], '6Haab': [843], 'B.B.': [844], 'Antibody': [845], 'arrays': [846], 'cancer': [848], 'research.Mol.': [849], '377-383Abstract': [854], '(215)': [862], 'array': [866], 'formats': [867], '(on': [868], 'planar': [869], 'substrates,': [870], 'beads,': [871], 'etc.),': [872], 'although': [873], 'significant': [874], 'problems': [875], 'remain': [876], 'production': [879], 'suitable': [881], 'simultaneous': [886, 1362], 'optimization': [887], 'fluid': [893, 1818], 'volume.': [894], 'Mass': [895, 1855, 1931, 3748], 'relying': [902], 'discriminating': [905], 'power': [906], 'analyzers': [909], 'ion': [917, 2347, 2624, 3650, 3797], 'current': [918], 'quantitation.': [921], 'field': [924], 'analytical': [926, 1325], 'chemistry,': [927], 'many': [928, 2019, 2085], 'small': [929], '(e.g.': [932, 1301, 1346], 'drug': [933, 949], 'metabolites': [934, 1300], '(7Kostiainen': [935], 'Kotiaho': [937], 'Kuuranne': [939], 'Auriola': [941], 'S.': [942, 1508, 1791, 2303], 'Liquid': [943], 'chromatography/atmospheric': [944], 'pressure': [945], 'ionization-mass': [946], 'metabolism': [950, 1312], 'studies.J.': [951], 'Mass.': [952], 'Spectrom.': [953], '2003;': [954, 1037, 1337, 1510], '38:': [955], '357-372Crossref': [956], '(318)': [959], 'Scholar),': [961, 1002, 1042, 1596, 1640, 1683, 2238, 2312, 2787], 'hormones': [962], '(8Tai': [963], 'S.S.': [964], 'Bunk': [965], 'D.M.': [966, 2759, 4218], 'White': [967], 'E.T.': [968], 'Welch': [969], 'M.J.': [970], 'Development': [971], 'evaluation': [973], 'reference': [976], 'measurement': [977, 1297, 1393, 2042, 2117], 'procedure': [978], 'determination': [981, 1062, 1363], 'total': [983, 3268, 4324], '3,3′,5-triiodothyronine': [984], 'isotope-dilution': [989], 'liquid': [990, 1053, 1355], 'chromatography-tandem': [991, 1356], 'spectrometry.Anal.': [993], '76:': [996], '5092-5096Crossref': [997], '(72)': [1000], 'degradation': [1004], 'products': [1005], '(9Ahmed': [1006], 'N.': [1007], 'Thornalley': [1008], 'P.J.': [1009], 'screening': [1011, 1303, 1330], 'early': [1016, 2930], 'glycation,': [1017, 1019], 'advanced': [1018], 'oxidation': [1020], 'nitrosation': [1022], 'cellular': [1024], 'extracellular': [1026], 'tandem': [1029, 1057, 1502], 'monitoring.Biochem.': [1034], 'Soc.': [1035], 'Trans.': [1036], '31:': [1038], '1417-1422Crossref': [1039], 'pesticides': [1044, 1068, 1259], '(10Sannino': [1045], 'Bolzoni': [1047], 'L.': [1048, 3230], 'Bandini': [1049], 'Application': [1051], 'chromatography': [1054, 1757, 1777, 2320], 'electrospray': [1056, 1103, 3580, 4032], 'processed': [1070], 'fruits': [1071], 'vegetables.J.': [1073], 'Chromatogr.': [1074, 1269], '1036:': [1077], '161-169Crossref': [1078], '(227)': [1081], 'Scholar))': [1083, 1342, 1517], 'routinely': [1085], 'measured': [1086, 1727, 2024, 2088], 'this': [1088, 1192, 1449, 1733, 2109, 2568, 2801, 2944, 3236], 'throughput': [1092, 2056], 'great': [1094], '(CV': [1096], '5%).': [1098], 'Most': [1099], 'use': [1102, 2128], 'ionization': [1104, 4033], 'followed': [1105], 'stages': [1108], 'selection:': [1111], 'first': [1113, 2601, 2906, 3985], 'stage': [1114, 1138], '(MS1)': [1115], 'selecting': [1116, 1140, 4156], 'intact': [1121], '(parent': [1123], 'ion)': [1124], 'and,': [1125], 'after': [1126], 'fragmentation': [1127, 3411], 'parent': [1130, 2648, 3796], 'collision': [1132, 3799], 'gas': [1134], 'atoms,': [1135], 'second': [1137, 2975, 3971], '(MS2)': [1139], 'fragment': [1143, 1542], 'parent,': [1146], 'collectively': [1147], 'generating': [1148], '(plural': [1153], 'MRM)': [1154], 'assay.': [1155], 'filters': [1159], 'produce': [1160], 'very': [1162], 'sensitive': [1165, 2133], 'response': [1166], 'integrate': [1178], 'peak': [1180, 2643, 2851, 3322, 4092], 'simple': [1183], 'one-dimensional': [1184], 'chromatographic': [1185, 2642], 'separation': [1186, 2420, 3964], 'sample.': [1189], 'principle,': [1191, 1526], 'MS-based': [1193], 'absolute': [1197, 1216, 1897, 3439, 3463], 'structural': [1198, 1898], 'analyte,': [1202], 'appropriate': [1207], '(SISs),': [1212], 'it': [1213, 1467, 2049], 'concentration.': [1220, 1524], 'These': [1221, 2829, 3398, 4247], 'have': [1223, 1725, 1761, 1994, 2206, 3373, 3384], 'been': [1224, 1389, 1726, 1763, 1902], '30': [1228, 2572, 3251, 3258, 3280, 3713, 3842], 'run': [1235, 3972], '(11Barr': [1236], 'D.B.': [1237, 1850, 1926, 3743], 'Barr': [1238], 'J.R.': [1239, 1600, 2722, 4181], 'Maggio': [1240, 1601], 'V.L.': [1241, 1602], 'Whitehead': [1242], 'Jr.,': [1243, 1604], 'R.D.': [1244, 2406, 2720, 4179], 'Sadowski': [1245], 'M.A.': [1246], 'Whyatt': [1247], 'R.M.': [1248], 'Needham': [1249, 1616], 'L.L.': [1250, 1617], 'multi-analyte': [1252], 'method': [1253, 1360, 1968, 2775, 4122, 4234], 'quantification': [1256, 1493, 1623, 1696, 3440], 'contemporary': [1258], 'high-resolution': [1266], 'spectrometry.J.': [1268, 1714], 'Anal.': [1271], 'Technol.': [1272], 'Biomed.': [1273], 'Life': [1274], 'Sci.': [1275, 1506, 2301], '778:': [1277], '99-111Crossref': [1278], '(148)': [1281], 'Such': [1284], 'slowly': [1287], 'gaining': [1288], 'acceptance': [1289], 'laboratory': [1293], 'routine': [1296, 1359, 2113], 'endogenous': [1299], 'newborns': [1304], 'panel': [1307], 'inborn': [1309], 'errors': [1310], '(12Roschinger': [1313], 'Olgemoller': [1315], 'Fingerhut': [1317], 'Liebl': [1319], 'Roscher': [1321], 'A.A.': [1322], 'Advances': [1323], 'improve': [1329, 3912], 'inherited': [1332], 'metabolic': [1333], 'diseases.Eur.': [1334], 'Pediatr.': [1336], '162:': [1338], 'S67-S76Crossref': [1339], 'some': [1344, 2213], 'drugs': [1345], 'immunosuppressants': [1347], '(13Streit': [1348], 'F.': [1349, 1797, 2761, 4220], 'Armstrong': [1350], 'V.W.': [1351], 'Oellerich': [1352], 'Rapid': [1354], 'sirolimus,': [1365], 'everolimus,': [1366], 'tacrolimus,': [1367], 'cyclosporin': [1369], 'blood.Clin.': [1373], '48:': [1376], '955-958Crossref': [1377], '(184)': [1380], 'Scholar)).': [1382, 1881, 4246], 'Recently': [1383], 'applied': [1390], 'mixtures': [1399], 'case,': [1450], 'stoichiometric': [1460], 'representative': [1461, 3696], 'from': [1465, 1498, 2072, 2157, 2161, 2295, 2386, 2466, 2541, 2571, 2695, 2794, 2984, 3000, 3084, 3095, 3257, 3392, 3710, 3898, 3924, 3997, 4307], 'cleaved': [1469], 'against': [1472, 2883], 'spiked': [1474, 3507], 'standard': [1476], '(a': [1477, 3267, 3585], 'synthetic': [1478, 3247], '(14Gerber': [1482], 'S.A.': [1483], 'Rush': [1484], 'Stemman': [1486], 'O.': [1487], 'Kirschner': [1488], 'M.W.': [1489], 'Gygi': [1490], 'S.P.': [1491], 'phosphoproteins': [1497], 'cell': [1499], 'lysates': [1500], 'MS.Proc.': [1503], 'Natl.': [1504, 2299], 'Acad.': [1505, 2300], 'U.': [1507, 2302], '100:': [1511], '6940-6945Crossref': [1512], '(1549)': [1515], 'yield': [1519], 'measure': [1521], 'requires': [1530], 'only': [1531, 3010, 3875, 4089], 'knowledge': [1532], 'masses': [1535, 3387], 'its': [1541, 2351], 'ions': [1543, 2616, 3782, 3961, 3981], 'ability': [1546], 'make': [1548], 'version.': [1552], 'apolipoprotein': [1597, 1630], 'A-I': [1598], '(15Barr': [1599], 'Patterson': [1603], 'D.G.': [1605], 'Cooper': [1606], 'G.R.': [1607], 'Henderson': [1608], 'L.O.': [1609], 'Turner': [1610], 'W.E.': [1611], 'Smith': [1612, 2405], 'S.J.': [1613, 3437], 'Hannon': [1614], 'W.H.': [1615], 'Sampson': [1618], 'E.J.': [1619], 'Isotope': [1620], 'dilution-mass': [1621], 'spectrometric': [1622, 1856, 1932, 3749], 'proteins:': [1626], 'model': [1627, 1672, 1699, 2337], 'application': [1628, 2110, 2352], 'A-I.Clin.': [1631], '1996;': [1633], '42:': [1634], '1676-1682Crossref': [1635], '(321)': [1638], 'growth': [1642, 1668], 'hormone': [1643, 1669], '(16Wu': [1644], 'S.L.': [1645], 'Amato': [1646], 'Biringer': [1648], 'Choudhary': [1650], 'G.': [1651], 'Shieh': [1652], 'P.': [1653], 'Hancock': [1654], 'W.S.': [1655], 'Targeted': [1656], 'low-level': [1659], 'LC/MSn:': [1665], 'system.J.': [1673], 'Proteome': [1674, 1715, 1872, 1948, 2658, 2682, 3765], 'Res.': [1675, 1716, 1873, 1949, 2683, 3766], '459-465Crossref': [1678], '(123)': [1681], 'prostate-specific': [1685, 1701], 'antigen': [1686, 1702], '(17Barnidge': [1687], 'D.R.': [1688], 'Goodmanson': [1689], 'M.K.': [1690, 2724, 3433, 4183], 'Klee': [1691], 'G.G.': [1692], 'Muddiman': [1693], 'D.C.': [1694], 'cleavage': [1709], 'dilution': [1712], '644-652Crossref': [1719], '(244)': [1722], 'Because': [1735, 3895, 4095], 'spectrometer': [1745, 1890], 'capacity': [1751], 'resolution': [1753, 3677], 'assisting': [1756], 'separation(s),': [1758], 'hybrid': [1759, 3647], 'also': [1762, 2123, 2587, 3135], 'coupling': [1765], 'immunodepletion': [1773], 'size': [1775, 2827, 4164], 'exclusion': [1776], '(18Liao': [1778], 'Kuhn': [1782], 'Chin': [1784], 'Chang': [1786], 'Jones': [1788], 'M.D.': [1789], "O'Neil": [1790], 'Clauser': [1792], 'K.R.': [1793, 2289], 'Hasler': [1796], 'Roubenoff': [1798], 'B.C.': [1803], 'Use': [1804], 'identify': [1809, 2735, 4194], 'disease': [1813, 2217], 'severity': [1814], 'synovial': [1817], 'arthritis.Arthritis': [1825], 'Rheum.': [1826], '50:': [1828], '3792-3803Crossref': [1829], '(239)': [1832], 'antibody': [1840, 2012], '(SISCAPA': [1842], '(19Anderson': [1843, 1919, 3736], 'Haines': [1847, 1923, 3740], 'L.R.': [1848, 1924, 3741], 'Hardie': [1849, 1925, 3742], 'Olafson': [1851, 1927, 3744], 'R.W.': [1852, 1928, 3745], 'Pearson': [1853, 1929, 3746], 'T.W.': [1854, 1930, 3747], '(SISCAPA).J.': [1871, 1947, 3764], '235-244Crossref': [1876, 1952, 3769], '(696)': [1879, 1955, 3772], 'essence': [1883], 'latter': [1885], 'uses': [1887], '"second': [1893], 'antibody"': [1894], 'specificity.': [1899, 3687], 'SISCAPA': [1900, 2136], 'shown': [1903, 3519], 'extend': [1905], 'least': [1914, 4297], '2': [1915, 3022], 'further': [1960, 2584], 'development': [1961], 'capable': [1963], 'extending': [1965], 'cover': [1970], 'full': [1972, 2855, 4111], 'known': [1973], '(i.e.': [1978, 3350, 4031], 'level).': [1982], 'compelling': [1985], 'evidence': [1986], 'value': [1995, 4025], 'there': [2002], 'may': [2003, 2015], 'applications': [2005], 'even': [2010], 'without': [2011, 2094, 3573], 'enrichment.': [2013], 'One': [2014], 'therefore': [2016], 'ask': [2017], 'how': [2018, 2034, 2084], 'quantitating': [2026], 'their': [2027], 'digest,': [2032, 4259], 'precise': [2035], 'be?': [2039], 'If': [2040], 'strategy': [2043], 'proves': [2044], 'robust,': [2047], 'carried': [2051, 2604], 'out': [2052, 2605], 'platforms?': [2058], 'To': [2059, 2866, 3460], 'address': [2060], 'questions': [2062], 'we': [2063, 2603, 2654, 2705, 2908, 2935, 2977, 3909], 'generated': [2064, 2265, 2477, 2819, 4146], 'tested': [2066, 4251], 'based': [2069, 2590, 3284, 3731], 'variety': [2074, 2796], 'see': [2083], 'effectively': [2089], 'subtraction': [2095], 'abundant': [2099, 4022], 'proteins.': [2100, 2141, 2799], 'An': [2101, 2335, 2376, 2470], 'understanding': [2102], 'performance': [2105], 'enable': [2112], 'relatively': [2115], 'inexpensive': [2116], 'classical': [2119, 2606], 'foundation': [2126], 'antibody-enhanced': [2135], 'We': [2142, 2586], 'set': [2145, 2193, 2389, 2453, 2931, 2976, 4142, 4315, 4344], 'MRMs': [2147, 2186, 2817, 2830, 2934, 2952, 2961, 2994, 3040, 3775, 3889, 4045, 4144, 4248, 4279, 4318], 'iteratively': [2148], 'three': [2150], 'basic': [2151], 'approaches:': [2152], 'pure': [2153], 'silico': [2155, 2190, 3289, 3916, 4352], 'design': [2156, 2160, 3291, 3524, 3920, 4040, 4121], 'sequence': [2158, 2509, 2805, 2860], 'databases,': [2159], 'available': [2162], 'proteomic': [2164, 2596], 'comprehensive': [2168, 4117], 'testing': [2170], 'candidate': [2175], 'protein.': [2179, 2469, 3397], 'our': [2181, 2959, 3030, 3286], 'initial': [2182, 3287, 3492, 3690], 'generate': [2185, 2901], 'purely': [2188], 'methods,': [2191], '177': [2195], 'forms': [2199], 'assembled': [2201, 4320], 'markers': [2211], 'aspect': [2214], 'cardiovascular': [2216, 2228], '(20Anderson': [2218], 'search': [2224, 4080], 'disease.J.': [2229], 'Physiol.': [2230], '563:': [2232], '23-60Crossref': [2233], '(316)': [2236], 'subset': [2241], '62': [2243, 2530], 'estimate': [2250], 'normal': [2252, 3086, 3403, 3729], 'available.': [2256], 'Predicted': [2257], 'along': [2266], 'relevant': [2268], 'Swiss-Prot': [2269, 2503], 'annotations': [2270], 'series': [2273, 3205, 4151], 'computed': [2275, 3733], 'physicochemical': [2276], 'parameters:': [2277], 'e.g.': [2278], 'amino': [2279, 2296, 2545, 2553, 3706], 'acid': [2280, 2297, 3063], 'composition,': [2281], 'mass,': [2283], 'Hopp-Woods': [2284], 'hydrophilicity': [2285], '(21Hopp': [2286], 'Woods': [2288], 'Prediction': [2290], 'antigenic': [2293], 'determinants': [2294], 'sequences.Proc.': [2298], '1981;': [2305], '78:': [2306], '3824-3828Crossref': [2307], '(2912)': [2310], 'predicted': [2314, 2527, 4158, 4267], 'retention': [2315, 2341], 'time': [2316, 4277], 'reversed-phase': [2318, 2349, 3602], '(C18)': [2319], '(22Krokhin': [2321], 'O.V.': [2322], 'Craig': [2323, 2662], 'Spicer': [2325], 'V.': [2326], 'Ens': [2327], 'Standing': [2329], 'K.G.': [2330], 'Beavis': [2331, 2669], 'R.C.': [2332, 2670], 'Wilkins': [2333], 'J.A.': [2334], 'prediction': [2339], 'times': [2342, 3023], 'pair': [2348], 'HPLC:': [2350], 'mapping': [2356], 'off-line': [2358], 'HPLC-MALDI': [2359], 'MS.Mol.': [2360], '908-919Abstract': [2365], '(246)': [2373], 'index': [2377, 2472], 'likelihood': [2380], 'experimental': [2382, 3300, 3922, 4009], 'derived': [2385], 'data': [2388, 2452, 3526, 3635, 3923, 3967, 4011], 'reported': [2390], 'et': [2393, 2663, 3428], 'al.': [2394, 2664, 3429], '(23Adkins': [2395], 'Varnum': [2397], 'S.M.': [2398], 'Auberry': [2399], 'K.J.': [2400], 'Moore': [2401], 'R.J.': [2402, 3431], 'Angell': [2403], 'N.H.': [2404], 'Springer': [2407], 'D.L.': [2408], 'Toward': [2411], 'blood': [2414], 'analysis': [2417, 2608], 'multidimensional': [2419], 'spectrometry.Mol.': [2424], '947-955Abstract': [2429], '(719)': [2437], 'counting': [2441], '"hits"': [2446], 'divided': [2454], 'hits': [2459], 'frequently': [2463, 2700], 'same': [2468, 3007, 3386, 3872, 3888, 4052], 'overall': [2471], 'quality': [2475], 'according': [2478, 3108], 'formula': [2481, 3805], 'positive': [2484], 'weights': [2485, 2495], 'Pro,': [2487], 'KP,': [2488], 'RP,': [2489], 'DP': [2491], 'content': [2492], 'negative': [2494], 'Cys,': [2497], 'Trp,': [2498], 'Met,': [2499], 'chymotrypsin': [2500], 'sites,': [2501], 'certain': [2502], 'features': [2504, 3399], '(carbohydrate': [2505], 'attachment,': [2506], 'modified': [2507], 'residues,': [2508], 'conflicts,': [2510], 'genetic': [2512], 'variants),': [2513], 'less': [2516], 'than': [2517, 2521, 4106], '800': [2518], 'greater': [2520, 4105], '2000.': [2522], '3619': [2524], 'marker': [2532], 'candidates': [2533], '(6–497': [2534], 'per': [2536, 4280], 'target)': [2537], 'ranged': [2538], 'length': [2540], '1': [2542, 3020, 3473, 3497, 3727], '285': [2544], 'acids.': [2546], 'Within': [2547], 'useful': [2549, 4163], '8–24': [2552], 'acids,': [2554], '721': [2555], 'C-terminal': [2559, 2565, 2579, 3378], 'Lys': [2560, 2580], '690': [2562], 'Arg.': [2566], 'report,': [2569], 'ending': [2577], 'study.': [2585], 'types': [2593], 'direct': [2595], 'experiments.': [2598], 'case': [2602, 2907], 'major': [2615, 3956], 'observed': [2617], 'subjected': [2619, 3485], 'trap': [2625, 3651], 'capabilities': [2626], '4000': [2629, 3644], 'Q': [2630, 3645], 'TRAP': [2631, 3646], 'instrument.': [2632], 'identified': [2634, 3990, 4077], 'showing': [2636], 'best': [2638], 'signal': [2639], 'intensity': [2640], 'shape': [2644], 'given': [2647], 'selected.': [2651], 'addition,': [2653], 'Global': [2657], 'Machine': [2659], 'database': [2660, 4079], '(24Craig': [2665], 'Cortens': [2667], 'J.P.': [2668], 'Open': [2671, 3563], 'source': [2672, 3641], 'system': [2673, 3102], 'analyzing,': [2675], 'validating,': [2676], 'storing': [2678], 'identification': [2680], 'data.J.': [2681], '1234-1242Crossref': [2686], '(578)': [2689], '(multiple': [2702], 'experiments).': [2703], 'Finally': [2704], 'adaptation': [2708], 'MIDAS': [2711, 3047], 'workflow,': [2712], 'described': [2713, 3424], 'previously': [2714], 'discovering': [2716], 'post-translational': [2717], 'modifications': [2718], '(25Unwin': [2719, 4178], 'Griffiths': [2721, 4180], 'Leverentz': [2723, 4182], 'Grallert': [2725, 4184], 'Hagan': [2727, 4186], 'I.M.': [2728, 4187], 'Whetton': [2729, 4188], 'A.D.': [2730, 4189], 'Multiple': [2731, 2770, 4190, 4229], 'sites': [2736, 4195], 'phosphorylation': [2739, 4198], 'sensitivity.Mol.': [2742, 4201], '1134-1144Abstract': [2747, 4206], '(188)': [2755, 4214], '26Cox': [2758, 4217], 'Zhong': [2760, 4219], 'Du': [2762, 4221], 'Duchoslav': [2764, 4223], 'Sakuma': [2766, 4225], 'McDermott': [2768, 4227], 'J.C.': [2769, 4228], 'identifying': [2777, 4236], 'posttranslational': [2779, 4238], 'modifications.J.': [2780, 4239], 'Biomol.': [2781, 4240], 'Tech.': [2782, 4241], '16:': [2784, 4243], '83-90PubMed': [2785, 4244], 'look': [2789], 'measurable': [2791], 'digested': [2807, 3319], 'silico,': [2809], 'likely': [2810, 3788], 'y-ion': [2811, 4023, 4171], 'fragments': [2812, 3344, 3365, 3391, 3785, 4172], 'predicted,': [2814], 'theoretical': [2816], 'window.': [2828], 'then': [2832, 3116, 3168, 4047, 4250], 'scan': [2837, 2856, 3929, 4112], 'data-dependent': [2840], 'experiment': [2841], 'peaks,': [2846], 'resulting': [2849], 'examined': [2853, 3042], 'obtain': [2859], 'verification': [2861], 'hypothesized': [2864], 'peptide.': [2865], 'verify': [2867], 'designated': [2871], 'targets,': [2873], 'searched': [2877], 'BLASTP': [2879], 'exact': [2881], 'matches': [2882], 'genome-derived': [2885], 'human,': [2886], 'mouse,': [2887], 'rat': [2889], 'Ensembl': [2890, 2893], 'Blastview': [2894], '(www.ensembl.org/Homo_sapiens/blastview).': [2895], 'Two': [2896], 'pseudorandom': [2902], 'MRMs.': [2903, 3847, 4042], '100': [2910], 'MS1': [2911, 2945, 2980, 2986, 3026], 'values': [2912, 2940, 2981, 2987, 2997, 3004], 'distributed': [2913], 'randomly': [2914, 2942, 2983, 2999], '(by': [2915, 3886], 'Excel': [2917], 'RAND': [2918], 'function)': [2919], 'between': [2920, 2943, 3019], '408.5': [2921], '1290.2': [2923], '(the': [2924, 3046, 3312, 4175], 'maximum': [2925, 2948], 'minimum': [2927], 'real': [2933, 2951, 2960, 2993, 3002, 3034], 'tested)': [2936], 'paired': [2937, 2978, 3025], 'MS2': [2939, 2996, 3003, 3015], 'chosen': [2941, 2982, 2998, 3709], '(1495.6),': [2953], 'mimicking': [2955], 'properties': [2957], '(which': [2962], 'generally': [2964], '+2': [2965], 'charge': [2966, 2971, 4018], 'state': [2967], '+1': [2970], 'fragments).': [2972], '131': [2979], 'among': [2985], 'table': [2991, 3564], 'list,': [3008], 'imposing': [3009], 'constraint': [3012], '(to': [3028], 'approximate': [3029], 'selection': [3031, 3694, 3938], 'criteria': [3032, 3939], 'MRMs).': [3035], 'Peaks': [3036], 'triggering': [3044, 4058], 'workflow).': [3048], 'following': [3050], 'chemicals': [3051], 'used:': [3053], 'trypsin': [3054], '(Promega),': [3055], 'sodium': [3056], 'dodecyl': [3057], 'sulfate': [3058], '(Bio-Rad),': [3059], 'iodoacetamide': [3060, 3174], '(Sigma),': [3061, 3064, 3066], 'formic': [3062, 3615], 'tris-(2-carboxyethyl)phosphine': [3065, 3158], 'acetonitrile': [3068, 3612], '(Burdick': [3069], 'Jackson).': [3071], 'All': [3072], 'performed': [3075, 3974], 'aliquots': [3077], 'volunteer.': [3087], 'highest': [3090], 'depleted': [3094, 3140, 3511], '("MARS";': [3103], 'Agilent': [3104], 'Technologies': [3105], 'spin': [3106], 'columns)': [3107], "manufacturer's": [3111], 'protocol.': [3112], 'Depleted': [3113], 'exchanged': [3117], 'into': [3118, 3508], '50': [3119, 3479], 'mM': [3120, 3157, 3172, 3480], 'ammonium': [3121], 'bicarbonate': [3122], 'VivaSpin': [3125], 'concentrator': [3126], '(5000': [3127], 'molecular': [3128], 'weight': [3129], 'cutoff,': [3130], 'VivaScience).': [3131], 'Undepleted': [3132], 'desalted': [3136], 'before': [3137], 'digestion.': [3138], 'Both': [3139], 'undepleted': [3142], 'denatured': [3146], 'reduced': [3148], 'incubating': [3150], '0.05%': [3153, 3476], 'SDS': [3154], '5': [3156, 3199, 3495], '60': [3160, 4326], '°C': [3161, 3182], '15': [3163, 3178], 'min.': [3164], 'adjusted': [3169, 3890], '10': [3171], 'incubated': [3176, 3197], 'min': [3179], '25': [3181], 'dark.': [3185], 'Trypsin': [3186], 'added': [3188, 3210, 3574, 3856], 'aliquot': [3191, 3469], '(protease:protein': [3192], 'ratio': [3193, 4303], '1:20)': [3195], 'h': [3200], '37': [3202], '°C.': [3203, 3632], 'SIS': [3207, 3415, 4360], 'spiking': [3217], '"polySIS"': [3224], 'polyprotein.': [3225], '2L.': [3226], 'C.': [3229], 'Hunter,': [3231], 'manuscript': [3232], 'preparation.': [3234], 'Briefly': [3235], 'cell-free': [3241], 'transcription': [3242], 'translation': [3244], 'gene': [3248], 'coding': [3249], 'concatenated': [3252, 3448], 'sequences': [3255], '(derived': [3256], 'proteins)': [3260], 'presence': [3263], 'U-13C6,U-15N2-labeled': [3265], 'lysine': [3266], 'increment': [3270], '8': [3272, 3483], 'amu': [3273], 'peptide).': [3278], 'not': [3296, 3315, 4128], 'fully': [3297], 'data.': [3301], 'peptides,': [3304, 3860], '13': [3305], 'present': [3310], 'studies': [3311], 'remainder': [3313], 'reproducibly': [3316], 'area': [3323], '>1E+04).': [3324], 'positioning': [3326], 'label': [3329, 3893], 'atoms': [3330], 'C': [3334, 3348], 'terminus': [3335, 3349, 3370], 'effect': [3341], 'contain': [3346, 3367], 'y-ions)': [3352], 'will': [3353, 3383], 'show': [3354], 'shift': [3357], 'due': [3358], 'label,': [3361], 'whereas': [3362], 'N': [3369], '(and': [3371], 'hence': [3372], 'lost': [3374], 'residues:': [3379], 'b-series': [3381], 'ions)': [3382], 'corresponding': [3390], '(sample-derived)': [3395], '(shifted': [3400], 'y-ions': [3401, 3789], 'b-ions)': [3404], 'simplification': [3407], 'interpreting': [3409], 'patterns': [3412], 'comparison': [3418], 'similar': [3421], 'QCAT': [3422, 3445], 'concept': [3423], 'recently': [3425], 'Beynon': [3427], '(27Beynon': [3430], 'Doherty': [3432], 'Pratt': [3434], 'J.M.': [3435], 'Gaskell': [3436], 'Multiplexed': [3438], 'artificial': [3444], 'signature': [3449], 'peptides.Nat.': [3450], 'Methods.': [3451], '2:': [3453], '587-589Crossref': [3454], '(395)': [3457], 'determine': [3461], 'concentration': [3464, 3493, 4004], 'polySIS': [3466, 3504, 3575, 3821, 3838, 3859, 3865], 'protein,': [3467], 'diluted': [3471], 'M': [3474], 'urea,': [3475], 'SDS,': [3477], 'Tris,': [3481], 'pH': [3482], 'N-terminal': [3487], 'Edman': [3488], 'sequencing,': [3489], '±': [3496], 'pmol/μl.': [3498], 'final': [3517], 'concentrations': [3518, 3721], 'Table': [3521, 4363], 'I.Table': [3522], 'ISummary': [3523], '(experiments': [3528], 'A–F)ExperimentReplicate': [3529], 'runsSampleLC': [3530], 'systemEquivalent': [3531], 'volume': [3533], 'loadedLoad': [3534], 'factorPolySIS': [3535], 'spikeTotal': [3536], 'proteinNon-depleted': [3537], 'proteinsμlfmolA10Depleted': [3538], 'digestLC': [3540, 3543], 'Packings0.01111.3B10Whole': [3541], 'Packings0.011011.3C10Whole': [3544], 'digestEksigent0.00160.12.0D10Depleted': [3546], 'digestEksigent0.010.612.0E10Depleted': [3548], 'digestEksigent0.0333.33.36.0F1_14Depletion': [3550], '1,': [3551, 3554], '1Eksigent0.0111F1_24Depletion': [3553], '2Eksigent0.0111F2_14Depletion': [3556], '2,': [3557, 3560], '1Eksigent0.0111F2_24Depletion': [3559], '2Eksigent0.0111': [3562], 'tab': [3568], 'Plasma': [3569], 'analyzed': [3578], 'LC': [3583, 3594, 3948], 'Packings': [3584], 'division': [3586], 'Dionex,': [3588], 'Synnyvale,': [3589], 'CA)': [3590], 'Eksigent': [3592], 'nanoflow': [3593], 'systems': [3595], '(Table': [3596, 4334], 'I)': [3597], '75-μm-diameter': [3599], 'C18': [3600], 'PepMap': [3601], 'columns': [3603], '(LC': [3604], 'Packings)': [3605], 'eluted': [3607], 'gradients': [3609], '3–30%': [3611], '0.1%': [3614], 'acid.': [3616], 'column': [3618, 3628, 4361], 'oven': [3619], '(Keystone': [3620], 'Scientific,': [3621], 'Inc.)': [3622], 'maintain': [3626], 'temperature': [3629], '35': [3631], 'Electrospray': [3633], 'MS': [3634, 4113], 'collected': [3637, 3953], 'NanoSpray®': [3640], 'quadrupole/linear': [3649], 'instrument': [3652], '(Applied': [3653], 'Biosystems/MDS': [3654], 'Sciex),': [3655], 'peaks': [3658, 4072, 4288], 'integrated': [3660], 'procedures': [3663], 'Analyst': [3666], 'software': [3667, 4138], '1.4.1': [3668], '(IntelliQuan': [3669], 'algorithm).': [3670], 'transitions': [3672], 'acquired': [3674], 'unit': [3676], 'Q1': [3681], 'Q3': [3683], 'quadrupoles': [3684], 'maximize': [3686], 'assays,': [3700], '8–18': [3705], 'acids': [3707], 'spanning': [3715], 'broad': [3717], '(6.6': [3722], '×': [3723, 3809], '108': [3724], 'fmol/ml': [3728], 'concentration)': [3730], 'characteristics': [3734], 'alone': [3735], 'assuming': [3778], 'doubly': [3779, 3957], 'charged': [3780, 3960], 'above': [3790, 4132, 4309], 'm/z': [3792, 3810, 4024], '2+': [3795], 'energies': [3800], 'assigned': [3801], 'generic': [3804], '(CE': [3806], '0.05': [3808], '+': [3811], '5),': [3812], 'expressed': [3817], 'concatamer': [3820], 'containing': [3823], 'copies': [3825], 'labeled': [3829, 3864], '[U-13C6,U-15N2]lysine.': [3831], 'When': [3832, 3848, 4043], 'analyzed,': [3840], '19': [3861], 'still': [3868], '11': [3876], 'digest-derived': [3880], 'unlabeled': [3881], 'cognate': [3882], 'masses).': [3894], 'vary': [3903], 'detectability': [3906], 'ESI-MS,': [3908], 'attempted': [3910], 'upon': [3913], 'conventional': [3926], 'applying': [3936], 'detectability.': [3944], 'Using': [3945, 4135], '3-h': [3947], 'gradient,': [3949], 'scans': [3951, 4060, 4284], 'triply': [3959], 'across': [3962], 'information-dependent': [3966], 'acquisition,': [3968], 'time-filtered': [3976], 'exclusions': [3977], 'run.': [3986], 'combined': [3988], 'results': [3989, 4306], '54': [3991], 'ranging': [3994], 'albumin': [3998], 'fibronectin': [4001], '(normal': [4002], '∼300': [4006], 'μg/ml).': [4007], 'This': [4008, 4343], 'provided': [4012], 'explicit': [4013], 'information': [4014], 'selection,': [4017], 'state,': [4019], 'under': [4026], 'conditions': [4029], 'collisional': [4035], 'fragmentation),': [4037], 'allowing': [4038], 'analyze': [4050], 'subsequent': [4056], 'run,': [4057], 'any': [4062, 4287], 'signal,': [4064], 'chromatogram': [4075], 'expected.': [4082], 'chromatograms,': [4088], 'detected.': [4094], 'expected': [4102], 'achieved': [4108], 'explored': [4124], 'those': [4126], 'tool,': [4139], 'together': [4166], '"MIDAS"': [4176], 'workflow': [4177], 'unfractionated': [4257], 'grouped': [4260], 'panels': [4262], 'included': [4264], '(50–100': [4278], 'run),': [4281], 'triggered': [4285], 'observed.': [4289], '12': [4291], 'examined,': [4293], 'nine': [4294], 'usable': [4299], '(signal-to-noise': [4301], '(S/N)': [4302], '>20).': [4304], 'pooled,': [4312], 'covered': [4322], 'II;': [4335], 'seven': [4336], 'represented': [4339], 'peptides).': [4342], 'includes': [4345], '18': [4346], '(indicated': [4354], 'X': [4357], 'II):': [4364]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2056467018', 'counts_by_year': [{'year': 2024, 'cited_by_count': 17}, {'year': 2023, 'cited_by_count': 13}, {'year': 2022, 'cited_by_count': 14}, {'year': 2021, 'cited_by_count': 17}, {'year': 2020, 'cited_by_count': 18}, {'year': 2019, 'cited_by_count': 28}, {'year': 2018, 'cited_by_count': 40}, {'year': 2017, 'cited_by_count': 55}, {'year': 2016, 'cited_by_count': 61}, {'year': 2015, 'cited_by_count': 71}, {'year': 2014, 'cited_by_count': 93}, {'year': 2013, 'cited_by_count': 131}, {'year': 2012, 'cited_by_count': 139}], 'updated_date': '2024-12-27T03:14:36.459399', 'created_date': '2016-06-24'}