Title: A Second Gene Encoding a Putative Serine/Threonine Protein Kinase Which Enhances Spermine Uptake inSaccharomyces cerevisiae
Abstract: We have isolated a new gene (PTK2) which restores spermine uptake of a polyamine uptake-deficient mutant ofSaccharomyces cerevisiae(Kakinuma, Y., Maruyama, T., Nozaki, T., Wada, Y., Ohsumi, Y., and Igarashi, K., 1995,Biochem. Biophys. Res. Commun.216,985–992). In magnesium-limited medium, the cell growth of a spermine-sensitive polyamine uptake mutant transformed withPTK2recovered its sensitivity to spermine. The nucleotide sequence of thePTK2gene indicated that it is identical with the YJR059W open reading frame of chromosome X encoding a putative serine/threonine protein kinase. The deduced amino acid sequence of thePTK2gene product was 38 % identical and 55 % similar with that of thePTK1(POT1) gene product, a putative serine/threonine protein kinase, which was found to enhance spermine uptake of the same mutant. The results indicate that polyamine transport of yeast is regulated by multiple phosphorylation/dephosphorylation pathways.
Publication Year: 1996
Publication Date: 1996-11-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 47
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