Title: Hexosamine interference with the determination of protein by the Lowry procedure
Abstract: 1. d-glucosamine and d-galactosamine and their corresponding N-acetyl derivatives interfere in the determination of protein by the Lowry assay. 2. At the level of 0.5 mg/ml of free hexosamine, d-glucosamine gave an apparent protein level of 0.055 mg/ml and d-galactosamine, 0.070 mg/ml. At 2.0 mg/ml hexosamine, the respective values were 0.128 mg/ml and 0.220 mg/ml. 3. The N-acetyl derivatives yielded approximately one-fifth of these values. 4. Chondroitin 6-sulfate does not interfere with the Lowry protein assay.
Publication Year: 1971
Publication Date: 1971-08-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 19
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