Title: Purification and radioimmunoassay of human alpha-1-fetoprotein: The effect of aggregates on the radioimmunoassay
Abstract: A simple method for the extraction of α1-fetoprotein from fetal serum in high yield and of sufficient purity for use as radio-iodinated tracer in a radio-immunoassay is described. The purified α1 -fetoprotein formed aggregates during storage. The presence of 125I-labelled aggregates affected the double antibody radioimmunoassay by increasing the non-specific bound, resulting in decreased assay sensitivity. Freshly prepared 125I-labelled monomer α1 -fetoprotein is prepared for each assay. The radioimmunoassay described is sensitive to between 100 and 200 pg per tube. Normal adult serum levels were found to be in the range from less than 1 μg/1 to 16 μg/1.
Publication Year: 1976
Publication Date: 1976-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 15
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot