Title: A collagen of unusual composition and a glycoprotein isolated from canine glomerular basement membrane
Abstract: In a recent study, Kefalides and Winzler (1964, 1965) reported the presence of a glycoprotein associated with a collagen-like protein in glomerular basement membrane of dogs. The basement membrane was solubilized by extraction with 8 M urea or by reduction and alkylation of disulfide bonds in 8 M urea. Amino acid analysis of basement membrane and its soluble fraction revealed the presence of a high hydroxylysine:hydroxyproline ratio (0.42 in basement membrane compared with 0.07 in mammalian collagen). Intact basement membrane contained 5.52% hexose, 1.4% glycosamine, 2.1% sialic acid, and 0.75% fucose. The amino acid and carbohydrate composition of the soluble fractions did not differ significantly from that of intact basement membrane. Dische etal. (1965) reported the presence of glucose and galactose linked to collagen and of a sialofucohexosaminoglycan in human glomerular basement membrane. The present report deals with the separation of two components from canine glomerular basement membrane. One is a glycoprotein and the other a collagen with a much higher hydroxyproline and hydroxylysine content than is found in other mammalian collagens.
Publication Year: 1966
Publication Date: 1966-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 82
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