Publication Information

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3681, 3733, 3780, 3802, 3806, 3810, 3814, 3821, 3842, 3882, 3895, 3918, 3971, 4010, 4019, 4024, 4033, 4037, 4043, 4059, 4064, 4069, 4073, 4085, 4089, 4102, 4105, 4136, 4151, 4159, 4217, 4230, 4245, 4251, 4282, 4286, 4292, 4312, 4342, 4348, 4354, 4365, 4370, 4375], 'nucleus': [6, 277, 819, 3334, 3843, 3972, 4293, 4366], 'contain': [7, 148, 278, 419, 3630, 3650], 'nuclear': [8, 26, 78, 123, 172, 279, 297, 349, 394, 443, 559, 568, 686, 693, 707, 774, 783, 810, 858, 955, 983, 987, 1084, 1130, 1338, 1518, 1572, 1642, 1784, 1941, 2000, 2023, 3756, 3900, 3983, 4097, 4122, 4283, 4322, 4339], 'localization': [9, 280, 775, 784, 1470, 2566, 2609, 2642, 3343, 3757, 3781, 3901], 'signals': [10, 281, 776, 1086], '(NLSs)': [11, 282, 777], 'that': [12, 29, 90, 103, 117, 154, 207, 223, 283, 300, 361, 374, 388, 425, 478, 494, 561, 696, 853, 1272, 1361, 1438, 1458, 1477, 1567, 1775, 1989, 2030, 2043, 3114, 3456, 3466, 3755, 3898, 3914, 3980, 4088, 4133, 4216, 4274, 4353, 4368], 'are': [13, 197, 284, 468, 722, 1027, 1461, 1472, 1481, 1568, 1824, 2019, 2172, 3377, 3467, 3558, 3688, 3879, 3892, 3920], 'recognized': [14, 43, 92, 285, 314, 363], 'by': [15, 44, 93, 286, 315, 364, 557, 675, 963, 1029, 1268, 1483, 2762, 2767, 2877, 2885, 3294, 3615, 3660, 3752], 'receptors': [17, 288, 766, 1137], 'termed': [18, 87, 289, 358, 567, 995, 3475], 'karyopherins': [19, 290], 'or': [20, 203, 242, 291, 474, 513, 1019, 1160, 1323, 1391, 2102, 2722, 2802, 2970, 3029, 3032, 3041, 3049, 3076, 3093, 3118, 3335, 3633, 3740, 3785, 3791, 4067, 4123, 4175, 4197, 4250, 4378], 'importins.': [21, 292], 'Until': [22, 293], 'recently,': [23, 294], 'only': [25, 296, 1138, 3123], 'sequence': [28, 125, 157, 299, 396, 428, 1096, 1159, 1959, 1992, 2034, 3189, 3222, 3256, 3460, 3474, 3508, 3569, 3582, 3596, 3613, 4013, 4039, 4091, 4153, 4219, 4232, 4254, 4332, 4356], 'had': [30, 301], 'been': [31, 302, 959, 1077, 1144, 1780, 1788, 3832], 'well': [32, 303, 979], 'defined': [33, 304, 1559], 'and': [34, 49, 65, 111, 144, 169, 174, 181, 305, 320, 336, 382, 415, 440, 445, 452, 655, 709, 738, 805, 886, 1026, 1037, 1080, 1179, 1283, 1384, 1403, 1436, 1469, 1515, 1634, 1640, 1738, 1773, 1822, 1834, 1863, 1871, 1969, 1984, 1997, 2005, 2093, 2111, 2166, 2178, 2559, 2602, 2635, 2708, 2756, 2760, 2769, 2783, 2820, 2843, 2848, 2872, 2879, 2894, 2911, 2925, 2930, 2949, 2958, 2975, 2998, 3068, 3144, 3166, 3254, 3326, 3404, 3418, 3461, 3577, 3581, 3591, 3663, 3686, 3748, 3760, 3838, 3877, 3890, 3904, 3976, 3997, 4002, 4042, 4072, 4104, 4126, 4163, 4367], 'characterized': [35, 306, 980, 1332], 'was': [36, 307, 1279, 1950, 2517, 2673, 2862, 2883, 2909, 2915, 3001, 3170, 3181, 3267, 3787, 3797, 3818, 4056, 4082, 4259, 4275, 4308], 'classical': [38, 309, 787, 986, 1010, 1074, 1129], 'NLS': [39, 310, 1035, 1040, 1328, 1372, 1378, 1387], '(cNLS),': [40, 311], 'which': [41, 146, 160, 191, 312, 417, 431, 462, 731, 964, 1005, 1501], 'is': [42, 91, 106, 120, 166, 175, 313, 362, 377, 391, 437, 446, 548, 562, 1373, 1497, 1632, 1732, 1739, 1939, 1995, 2006, 2035, 3395, 3513, 3586, 3906, 3967, 3985, 4016, 4094, 4129, 4156, 4222, 4235, 4359], 'importin': [45, 316, 996, 1003], 'α.': [46, 317], 'However,': [47, 318, 681, 1257, 2011], 'Chook': [48, 319, 1282, 1295, 1805, 3204, 3237, 3488], 'coworkers': [50, 321, 1284], '(Lee,': [51, 322], 'B.': [52, 323, 572, 591], 'J.,': [53, 324], 'Cansizoglu,': [54, 325], 'A.': [55, 326, 821, 1043, 1098, 1195, 2122, 2214, 2252, 3273, 3277], 'E.,': [56, 59, 327, 330], 'Süel,': [57, 328], 'K.': [58, 329, 1203, 1222], 'Louis,': [60, 331], 'T.': [61, 332, 1189, 1205, 2128], 'H.,': [62, 333], 'Zhang,': [63, 334], 'Z.,': [64, 335], 'Chook,': [66, 337], 'Y.': [67, 338, 893, 1207], 'M.': [68, 339, 825, 863, 897, 1049, 1104, 1199], '(2006)': [69, 340], 'Cell': [70, 341, 578, 596, 617, 749, 866], '126,': [71, 342], '543–558)': [72, 343], 'have': [73, 344, 1076, 1143, 1779, 1787], 'provided': [74, 345], 'new': [75, 267, 346, 538, 2069, 2497], 'insight': [76, 347], 'targeting': [79, 124, 247, 350, 395, 518, 771, 1085, 4340], 'with': [80, 211, 239, 351, 482, 510, 564, 808, 1007, 1169, 1345, 1829, 2077, 2532, 2584, 2596, 2714, 2741, 2771, 2787, 2812, 2826, 2867, 2904, 2919, 2927, 2951, 3164, 3183, 3270, 3383, 3732, 3799, 3824, 4120, 4172, 4188, 4324, 4341], 'their': [81, 352, 1083, 2546, 4127], 'identification': [82, 353, 2053], 'of': [83, 131, 218, 230, 234, 258, 354, 402, 489, 501, 505, 529, 630, 672, 684, 692, 724, 814, 857, 946, 951, 973, 982, 1016, 1022, 1073, 1082, 1092, 1141, 1148, 1262, 1266, 1314, 1329, 1358, 1395, 1410, 1441, 1463, 1492, 1563, 1625, 1628, 1688, 1694, 1729, 1836, 1947, 1955, 1963, 1967, 1981, 2002, 2025, 2049, 2054, 2072, 2081, 2085, 2574, 2617, 2656, 2750, 2774, 2835, 2890, 2897, 2993, 2995, 3125, 3261, 3393, 3412, 3509, 3520, 3556, 3610, 3619, 3692, 3738, 3758, 3782, 3805, 3813, 3902, 3917, 4021, 4036, 4040, 4092, 4099, 4135, 4240, 4255, 4302, 4357], 'a': [84, 121, 255, 263, 355, 392, 526, 534, 558, 848, 883, 990, 1093, 1139, 1170, 1258, 1263, 1269, 1315, 1356, 1366, 1380, 1389, 1396, 1439, 1449, 1486, 1617, 1626, 1979, 2078, 2496, 2520, 2533, 2587, 2599, 2679, 2815, 2829, 2936, 2946, 3004, 3046, 3141, 3191, 3224, 3340, 3380, 3388, 3616, 3651, 3661, 3664, 4241, 4272], 'novel': [85, 356, 1957], 'NLS,': [86, 357, 782], 'PY-NLS,': [89, 360, 3263, 3604], 'human': [95, 236, 366, 507, 1316, 1429, 1932], 'karyopherin': [96, 367, 880, 936, 966, 992, 1135, 1172, 1317, 1320], 'β2/transportin': [97, 368], '(Kapβ2)': [98, 369, 1322], 'receptor.': [99, 370], 'Here,': [100, 371], 'we': [101, 250, 372, 521, 1987, 2075, 4165], 'demonstrate': [102, 373, 1476, 2029], 'PY-NLS': [105, 119, 164, 195, 220, 226, 376, 390, 435, 466, 491, 497, 1443, 1958, 2032, 2057, 2087, 3185, 3193, 3226, 3477, 3641], 'conserved': [107, 378, 2014, 2036], 'in': [108, 126, 133, 189, 227, 379, 397, 404, 460, 498, 554, 657, 701, 712, 735, 742, 953, 1164, 1448, 1452, 1466, 1485, 1494, 1644, 1701, 1744, 1771, 1826, 1866, 1960, 1975, 2037, 2058, 2088, 2130, 2169, 2174, 2181, 2495, 2507, 2552, 2556, 2581, 2593, 2624, 2630, 2675, 2738, 2809, 2823, 2846, 2850, 2864, 2990, 3060, 3066, 3140, 3298, 3379, 3387, 3561, 3597, 3627, 3643, 3676, 3743, 3789, 3836, 3885, 3973, 3987, 4032, 4049, 4110, 4178, 4291], 'Saccharomyces': [109, 380, 736], 'cerevisiae': [110, 381, 737, 1496, 2039, 3301], 'show': [112, 383], 'first': [115, 386], 'time': [116, 387, 3149], 'functional': [122, 393, 1468, 2056], 'vivo.': [127, 398, 1961], 'The': [128, 399, 935, 1400, 1489, 1945, 2514, 2906, 2941, 3375, 3506, 3553, 3595, 3990, 4314, 4329], 'apparent': [129, 400, 1490], 'ortholog': [130, 401, 1491], 'Kapβ2': [132, 403, 1351, 1368, 1418, 1434, 1447, 1484, 1493, 1793, 3472], 'yeast,': [134, 405], 'Kap104,': [135, 406, 4164], 'has': [136, 407, 732, 958, 1087, 1379, 1388, 1502, 1558, 3830], 'two': [137, 408, 1020, 1503, 3287, 3464, 3656], 'known': [138, 409, 1350, 1504, 3414], 'cargos,': [139, 410, 718, 1352, 1435, 1505], 'mRNA-binding': [141, 412, 1335, 1507], 'proteins': [142, 413, 1444, 1460, 1480, 1508, 1566, 2504, 2942, 2969, 3034, 3297, 3329, 3919, 4138, 4296], 'Hrp1': [143, 414, 1516, 1772, 1821, 1830, 1938, 1968, 1994, 2997, 3031, 3110, 3120, 3126, 3403, 3417, 3457, 3510, 3598, 3685, 3747, 3759, 3784, 3796, 3903, 3994, 4015, 4022, 4093, 4155, 4162, 4221, 4234, 4256, 4358], 'Nab2,': [145, 190, 416, 461, 1616], 'both': [147, 167, 418, 438, 1976, 3559, 3629, 3648, 3689, 3875, 3888, 3974, 4068], 'putative': [149, 420, 1367, 1433, 1972, 2070, 3625, 4011], 'PY-NLS-like': [150, 156, 187, 421, 427, 458, 1973, 1991, 2015, 3407, 3507, 3554, 3568, 3815, 3991, 4012, 4038, 4090, 4152, 4218, 4231, 4253, 4331, 4355], 'sequences.': [151, 422], 'We': [152, 423], 'find': [153, 424], 'within': [158, 429, 1993, 2017, 3523, 3655, 3970, 3993, 4014, 4154, 4220, 4233], 'Hrp1,': [159, 430, 1724], 'closely': [161, 432, 1935, 3600], 'matches': [162, 433], 'consensus,': [165, 196, 237, 436, 467, 508], 'necessary': [168, 439, 1474, 1633, 1996, 4017, 4095], 'sufficient': [170, 441, 1635, 1740, 1998, 4236, 4360], 'also': [176, 447, 688, 1696, 2041, 3912], 'required': [177, 199, 448, 470, 1569, 1733, 2007, 2021, 2651, 3530, 4157], 'receptor': [179, 450, 729, 968, 994, 1174, 1271, 3734, 4225], 'binding': [180, 451, 1149, 1177, 1455, 1622, 1637, 1698, 1735, 1778, 1985, 2670, 3473], 'protein': [182, 204, 453, 475, 768, 1277, 1336, 1513, 1731, 1769, 1933, 2004, 2009, 2501, 2682, 2881, 3410, 3522, 3807, 3829, 4273, 4344], 'function.': [183, 248, 454, 519, 2010], 'In': [184, 455, 542, 976, 1125, 1615, 1723, 2665, 2986, 3622, 3872], 'contrast,': [185, 456, 3623], 'sequences': [188, 459, 772, 1420, 1786, 1966, 1974, 2016, 3411, 3465, 3555, 3626, 3649], 'vary': [192, 463, 3638], 'from': [193, 464, 550, 2098, 2545, 2717, 3105, 3639, 4191], 'not': [198, 469, 1274, 2020, 3981, 4116, 4146], 'proper': [201, 472, 3899], 'function,': [205, 476, 1983], 'suggesting': [206, 477], 'Kap104': [208, 269, 479, 540, 1498, 1639, 1700, 1737, 1777, 2044, 2672, 3415, 3693, 3764, 4006], 'may': [209, 480, 3730], 'interact': [210, 481, 1168, 3731, 4171], 'different': [212, 483, 3184, 3736, 3745], 'cargos': [213, 484, 1008, 1156, 1267, 1794, 2071, 3416, 3691], 'using': [214, 485, 1175, 2510, 2519, 3003, 3150, 3345, 3735], 'multiple': [215, 486], 'mechanisms.': [216, 487], 'Dissection': [217, 488], 'consensus': [221, 492, 1095, 1412, 2033, 3188, 3612, 3642], 'reveals': [222, 493], 'minimal': [225, 496], 'yeast': [228, 260, 499, 531, 2059, 2065, 2164, 2715, 2791, 2892, 3291, 3348, 4179, 4189], 'consists': [229, 500], 'C-terminal': [232, 503, 1397, 3259, 3563, 3608, 3645, 3811, 4034, 4246, 4315, 4372], 'portion': [233, 504, 3609], 'R/H/KX2–5PY,': [238, 509], 'upstream': [240, 511, 1394, 3631, 4070, 4376], 'basic': [241, 512, 1023, 1382, 1392, 3225, 3617, 3632, 4377], 'hydrophobic': [243, 514, 1390, 3192, 3634, 4379], 'residues': [244, 515, 1405, 3659, 4076, 4380], 'enhancing': [245, 516], 'Finally,': [249, 520, 2052], 'apply': [251, 522], 'this': [252, 523, 665, 1948, 2170, 2182, 3827, 4182], 'analysis': [253, 524], 'to': [254, 525, 678, 878, 940, 999, 1089, 1127, 1326, 1415, 1446, 1475, 1638, 1699, 1736, 1741, 1832, 1937, 1951, 2062, 2067, 2118, 2565, 2608, 2641, 2659, 2832, 2945, 2964, 2981, 3058, 3134, 3285, 3331, 3339, 3469, 3528, 3657, 3808, 3834, 3839, 3841, 3881, 4058, 4079, 4084, 4118, 4206, 4237, 4261, 4270, 4278, 4289, 4311, 4318, 4334, 4347, 4361], 'bioinformatic': [256, 527], 'search': [257, 265, 528, 536], 'proteome': [261, 532, 2066, 3292], 'as': [262, 533, 2586, 2598, 2678, 2814, 2828, 3121, 3647], 'preliminary': [264, 535, 2079], 'potential': [268, 539], 'cargos.': [270, 541], 'eukaryotic': [543], 'cells,': [544, 3874, 3887, 3978], 'genetic': [546], 'material': [547], 'separated': [549], 'cellular': [552], 'machinery': [553, 695], 'cytoplasm': [556, 942], 'membrane': [560, 687, 2948], 'studded': [563], 'regulated': [565], 'channels': [566], 'pore': [569, 4284], 'complexes': [570], '(1Fahrenkrog': [571], 'Aebi': [573, 592, 913], 'U.': [574, 593, 912, 914], 'Nat.': [575], 'Rev.': [576, 829], 'Mol.': [577, 1242, 1604, 1676, 2697, 3956], 'Biol.': [579, 597, 750, 867, 1055, 1110, 1541, 1579, 1606, 1651, 1678, 1708, 1751, 1915, 2269, 2299, 2335, 2699, 3440, 3538, 3714, 3857, 3958], '2003;': [580, 868, 1209, 3367], '4:': [581], '757-766Crossref': [582], 'PubMed': [583, 601, 622, 646, 759, 834, 871, 902, 922, 1065, 1120, 1212, 1229, 1252, 1306, 1531, 1551, 1589, 1610, 1661, 1682, 1718, 1761, 1816, 1858, 1897, 1925, 2199, 2243, 2279, 2309, 2345, 2374, 2703, 3021, 3215, 3248, 3321, 3370, 3430, 3450, 3499, 3548, 3704, 3724, 3775, 3867, 3937, 3962], 'Scopus': [584, 602, 647, 760, 835, 872, 903, 923, 1066, 1121, 1213, 1230, 1253, 1307, 1532, 1552, 1590, 1611, 1662, 1683, 1719, 1762, 1817, 1859, 1898, 1926, 2200, 2280, 2310, 2346, 2375, 2704, 3022, 3216, 3249, 3322, 3371, 3431, 3451, 3500, 3549, 3705, 3725, 3776, 3868, 3938, 3963], '(336)': [585], 'Google': [586, 604, 623, 649, 762, 837, 874, 905, 925, 1068, 1123, 1215, 1232, 1255, 1309, 1534, 1554, 1592, 1613, 1664, 1685, 1721, 1764, 1819, 1861, 1900, 1928, 2202, 2244, 2282, 2312, 2348, 2377, 2706, 3024, 3218, 3251, 3283, 3324, 3373, 3433, 3453, 3502, 3551, 3707, 3727, 3778, 3870, 3940, 3965], 'Scholar,': [587, 605, 650, 906, 926, 1216, 1233, 1535, 1593, 1665, 1901, 2142, 3434, 3708, 3941], '2Stoffler': [588], 'D.': [589, 908, 2146], 'Fahrenkrog': [590], 'Curr.': [594], 'Opin.': [595, 865], '1999;': [598, 1226, 1543, 1581, 1653, 1710, 1753, 3442, 3540, 3716], '11:': [599, 1856, 2197, 2372], '391-401Crossref': [600], '(297)': [603], '3Allen': [606], 'T.D.': [607], 'Cronshaw': [608], 'J.M.': [609], 'Bagley': [610], 'S.': [611, 1187, 1495, 1848, 1879, 2038, 2089, 2364, 3300], 'Kiseleva': [612], 'E.': [613, 827, 1191, 1193, 1844, 2360, 2730, 3275], 'Goldberg': [614], 'M.W.': [615], 'J.': [616, 918, 1054, 1109, 1237, 1540, 1578, 1650, 1707, 1750, 1846, 1893, 1914, 2144, 2268, 2298, 2334, 2362, 3011, 3312, 3439, 3537, 3713, 3856], 'Sci.': [618], '2000;': [619], '113:': [620], '1651-1659Crossref': [621], 'Scholar).': [624, 763, 838, 875, 934, 1069, 1124, 1256, 1555, 1614, 1686, 1722, 1765, 1929, 2162, 3025, 3374, 3552, 3871], 'Nuclear': [625, 4000], 'pores': [626], 'prevent': [627], 'passive': [628], 'diffusion': [629], 'macromolecules': [631], 'larger': [632], 'than': [633, 3669], '∼40': [634], 'kDa': [635], '(4Paine': [636], 'P.L.': [637], 'Moore': [638, 1851, 2367], 'L.C.': [639, 3357], 'Horowitz': [640], 'S.B.': [641], 'Nature.': [642, 898, 1225, 3366], '1975;': [643], '254:': [644], '109-114Crossref': [645], '(578)': [648], '5Bonner': [651], 'W.M.': [652], 'Protein': [653], 'Migration': [654], 'Accumulation': [656], 'Nuclei.': [658], 'Academic': [659], 'Press,': [660, 2137, 2157], 'New': [661], 'York1978Google': [662], 'Scholar),': [663, 1820, 2707, 3219, 3252, 3728, 3779, 3966], 'so': [664], 'division': [666], 'can': [667, 697, 4381], 'facilitate': [668], 'precise': [670], 'management': [671], 'gene': [673], 'expression': [674, 2834], 'controlling': [676], 'access': [677], 'genome.': [680], 'presence': [683], 'necessitates': [689], 'involvement': [691], 'specifically': [698], 'recognize': [699, 767], 'cargo': [700, 769, 816, 888, 974, 1565, 2050], 'cytoplasm,': [703, 4086], 'mediate': [704, 806, 1275, 1742, 1776, 4238, 4362], 'transport': [705, 728, 859, 956, 1270], 'through': [706, 4281], 'pores,': [708], 'effect': [710], 'delivery': [711], 'nucleus.': [714, 4287, 4349], 'For': [715, 2538, 3099, 4181], 'many': [716, 1792], 'these': [719, 1155, 1417, 1423, 1442, 1478, 1564, 4294], 'factors': [721], 'members': [723, 734, 741], 'importin/karyopherin': [726], 'β': [727, 937, 967, 993, 997, 1136, 1173, 1318], 'superfamily,': [730], '14': [733], 'over': [739, 3148], '20': [740], 'humans': [743], '(6Mosammaparast': [744], 'N.': [745, 910, 1197], 'Pemberton': [746], 'L.F.': [747], 'Trends': [748], '2004;': [751, 2700, 3959], '14:': [752], '547-556Abstract': [753], 'Full': [754, 756, 1060, 1062, 1115, 1117, 1247, 1249, 1301, 1303, 1546, 1548, 1584, 1586, 1656, 1658, 1713, 1715, 1756, 1758, 1811, 1813, 1920, 1922, 2274, 2276, 2304, 2306, 2340, 2342, 3210, 3212, 3243, 3245, 3445, 3447, 3494, 3496, 3543, 3545, 3719, 3721, 3862, 3864], 'Text': [755, 757, 1061, 1063, 1116, 1118, 1248, 1250, 1302, 1304, 1547, 1549, 1585, 1587, 1657, 1659, 1714, 1716, 1757, 1759, 1812, 1814, 1921, 1923, 2275, 2277, 2305, 2307, 2341, 2343, 3211, 3213, 3244, 3246, 3446, 3448, 3495, 3497, 3544, 3546, 3720, 3722, 3863, 3865], 'PDF': [758, 1064, 1119, 1251, 1305, 1550, 1588, 1660, 1717, 1760, 1815, 1924, 2278, 2308, 2344, 3214, 3247, 3449, 3498, 3547, 3723, 3866], '(269)': [761], 'These': [764, 1013, 1454, 2027, 4113, 4350], 'bearing': [770], 'called': [773, 1319], '2The': [778], 'abbreviations': [779], 'used': [780, 2168, 2180, 3269, 4269], 'are:': [781], 'signal;': [785], 'cNLS,': [786], 'NLS;': [788], 'GFP,': [789], 'green': [790], 'fluorescent': [791, 2500], 'protein;': [792], 'GST,': [793], 'glutathione': [794], 'S-transferase;': [795], 'PBS,': [796], 'phosphate-buffered': [797], 'saline;': [798], 'HRP,': [799], 'horseradish': [800], 'peroxidase;': [801], '5-FOA,': [802], '5-fluoroorotic': [803, 3083], 'acid.': [804], 'interactions': [807, 3739], 'pore,': [811], 'allowing': [812], 'translocation': [813], '(7Cook': [820], 'Bono': [822], 'F.': [823, 2190], 'Jinek': [824], 'Conti': [826], 'Annu.': [828], 'Biochem.': [830], '2007;': [831, 1057, 1112, 3318], '76:': [832], '647-671Crossref': [833], '(413)': [836], 'Once': [839], 'inside': [840], 'nucleus,': [842, 4025], 'complex': [845], 'encounters': [846], 'RanGTP,': [847], 'small': [849, 4371], 'Ras': [850], 'family': [851], 'GTPase': [852], 'regulates': [854], 'directionality': [856], '(8Quimby': [860], 'B.B.': [861], 'Dasso': [862], 'Curr': [864], '15:': [869, 920], '338-344Crossref': [870], '(161)': [873], 'RanGTP': [876], 'binds': [877, 998], 'β,': [881], 'causing': [882], 'conformational': [884], 'change': [885, 3675], 'triggering': [887], 'release': [889], '(9Lee': [890], 'S.J.': [891], 'Matsuura': [892], 'Liu': [894], 'S.M.': [895, 1887], 'Stewart': [896, 1048, 1103], '2005;': [899], '435:': [900], '693-696Crossref': [901], '(170)': [904], '10Görlich': [907], 'Panté': [909], 'Kutay': [911], 'Bischoff': [915], 'F.R.': [916], 'EMBO': [917, 1892], '1996;': [919, 1528, 3427, 3701, 3772, 3934], '5584-5594Crossref': [921], '(533)': [924], '11Chook': [927], 'Y.M.,': [928], 'Blobel': [929, 1523, 3422, 3696, 3767, 3929], 'G.,': [930], 'Nature,': [931], '399,': [932], '230–237.Google': [933], 'then': [938, 1354, 1426, 2709, 3268], 'returns': [939], 'another': [944], 'round': [945], 'import.': [947], 'One': [948, 2888], 'main': [949], 'focus': [950], 'inquiry': [952], 'field': [957], 'understanding': [960], 'mechanism': [962, 1259], 'each': [965, 2749, 2994], 'recognizes': [969], 'its': [970, 1330], 'particular': [971, 1171, 4121], 'host': [972], 'proteins.': [975, 1977, 2839], 'most': [978, 1134, 1934, 2539], 'system': [981, 4301], 'transport,': [984], 'pathway,': [989, 1132], 'specific': [991, 1264, 1783, 4026], 'an': [1000, 1620, 2526, 2652, 3151, 4299], 'adaptor': [1001], 'protein,': [1002, 4243], 'α,': [1004], 'interacts': [1006], 'carrying': [1009], 'NLSs': [1011], '(cNLSs).': [1012], 'cNLSs': [1014], 'consist': [1015], 'either': [1017, 2952, 3038, 3116, 3332, 4063, 4173, 4194, 4244], 'one': [1018, 3459], 'clusters': [1021], 'amino': [1024, 3620, 4027], 'acids': [1025], 'exemplified': [1028], 'SV40': [1031], 'large': [1032, 1071, 4277], 'T': [1033], 'antigen': [1034], '(126PKKKRRV132)': [1036], 'nucleoplasmin': [1039], '(155KRPAATKKAGQAKKKK170)': [1041], '(12Lange': [1042, 1097], 'Mills': [1044, 1099], 'R.E.': [1045, 1100, 1599, 1671, 1875], 'Lange': [1046, 1101], 'C.J.': [1047, 1102], 'Devine': [1050, 1105], 'S.E.': [1051, 1106], 'Corbett': [1052, 1107, 1912, 2266, 2296, 2332, 3399, 3854], 'A.H.': [1053, 1108, 1913, 2267, 2297, 2333, 3855], 'Chem.': [1056, 1111, 1542, 1580, 1652, 1709, 1752, 1916, 2270, 2300, 2336, 3441, 3539, 3715, 3858], '282:': [1058, 1113], '5101-5105Abstract': [1059, 1114], '(861)': [1067, 1122], 'A': [1070, 2149, 3390, 3923], 'number': [1072], 'substrates': [1075, 1142, 1462], 'experimentally': [1078], 'verified,': [1079], 'examination': [1081], 'led': [1088, 1413], 'development': [1091], 'useful': [1094], 'contrast': [1126], 'handful': [1140], 'identified': [1145, 1431], 'making': [1146], 'generalization': [1147], 'schemes': [1150], 'difficult.': [1151], 'Compounding': [1152], 'problem,': [1154], 'often': [1157], 'lack': [1158], 'structural': [1161, 1343], 'homology': [1162], 'and,': [1163], 'some': [1165, 4325], 'cases,': [1166], 'even': [1167, 3742, 4369], 'distinct': [1176], 'sites': [1178], 'diverse': [1180], 'interaction': [1181, 4160], 'modes': [1182], '(for': [1183], 'example,': [1184], 'Refs.': [1185], '13Lee': [1186], 'Sekimoto': [1188], 'Yamashita': [1190], 'Nagoshi': [1192], 'Nakagawa': [1194], 'Imamoto': [1196], 'Yoshimura': [1198], 'Sakai': [1200], 'H.': [1201, 1601, 1673], 'Chong': [1202], 'Tsukihara': [1204], 'Yoneda': [1206], 'Science.': [1208, 1527, 3426, 3700, 3771, 3933], '302:': [1210], '1571-1575Crossref': [1211], '(173)': [1214], '14Cingolani': [1217], 'G.': [1218, 1235, 1524, 3013, 3423, 3697, 3768, 3930], 'Petosa': [1219], 'C.': [1220, 2192], 'Weis': [1221], 'Müller': [1223], 'C.W.': [1224], '399:': [1227], '221-229Crossref': [1228], '(452)': [1231], '15Cingolani': [1234], 'Bednenko': [1236], 'Gillespie': [1238], 'M.T.': [1239, 2321], 'Gerace': [1240], 'L.': [1241, 1889], 'Cell.': [1243, 1297, 1605, 1677, 1807, 2698, 3206, 3239, 3490, 3957], '2002;': [1244, 1894, 1917, 2271, 2301, 3280, 3859], '10:': [1245], '1345-1353Abstract': [1246], '(154)': [1254], 'underlying': [1260], 'recognition': [1261, 1419], 'class': [1265], 'does': [1273], 'cNLS': [1276], 'recently': [1280], 'determined.': [1281], '(16Lee': [1285, 1795, 3194, 3227, 3478], 'B.J.': [1286, 1796, 3195, 3228, 3479], 'Cansizoglu': [1287, 1797, 3196, 3229, 3480], 'A.E.': [1288, 1798, 2319, 3197, 3230, 3481], 'Süel': [1289, 1799, 3198, 3231, 3482], 'K.E.': [1290, 1800, 3199, 3232, 3483], 'Louis': [1291, 1801, 3200, 3233, 3484], 'T.H.': [1292, 1802, 3201, 3234, 3485], 'Zhang': [1293, 1803, 1908, 2262, 2292, 2685, 3202, 3235, 3486, 3850, 3944], 'Z.': [1294, 1804, 3203, 3236, 3487], 'Y.M.': [1296, 1806, 3205, 3238, 3489], '2006;': [1298, 1808, 2337, 3207, 3240, 3491], '126:': [1299, 1809, 3208, 3241, 3492], '543-558Abstract': [1300, 1810, 3209, 3242, 3493], '(424)': [1308, 1818, 3217, 3250, 3501], 'Scholar)': [1310, 1862, 3284, 3325, 3454, 3503], 'reported': [1311], 'structure': [1313], 'β2': [1321], 'transportin': [1324], 'bound': [1325, 1445, 2913, 4205], 'best': [1331], 'cargo,': [1333, 3925], 'heterogeneous': [1337, 1940], 'ribonucleoprotein': [1339, 1519, 1942], 'A1.': [1340], 'Combining': [1341], 'information': [1344], 'biochemical': [1346], 'studies': [1347], 'involving': [1348, 2645], 'other': [1349], 'they': [1353, 1425, 3637, 3729], 'proposed': [1355], 'set': [1357], 'predictive': [1359], 'rules': [1360], 'outline': [1362], 'requirements': [1364], 'NLS:': [1369], '1)': [1370, 1520], 'structurally': [1374], 'disordered,': [1375], '2)': [1376, 1514], 'net': [1381], 'charge,': [1383], '3)': [1385], 'region': [1393, 1618, 1693, 3525], 'R/H/KX2–5PY': [1398], 'motif.': [1399], 'invariant': [1401], 'proline': [1402, 3662], 'tyrosine': [1404], 'contained': [1406, 3560], 'at': [1407, 3089, 3130, 3161, 3172, 3515, 3801, 3820, 4141], 'end': [1409], 'them': [1414], 'term': [1416], '"PY-NLSs."': [1421], 'Using': [1422, 1978], 'rules,': [1424], 'searched': [1427], 'proteome,': [1430], '81': [1432], 'showed': [1437, 4211, 4320], 'selection': [1440], 'RanGTP-dependent': [1450], 'manner': [1451], 'vitro.': [1453], 'experiments': [1456, 1471], 'suggest': [1457], 'Kapβ2;': [1464], 'however,': [1465, 3884, 4061], 'vivo': [1467, 1645, 1745, 2991, 3837], 'still': [1473], 'PY-NLS-containing': [1479], 'imported': [1482], 'PY-NLS-dependent': [1487], 'manner.': [1488], '(karyopherin': [1499], '104),': [1500], 'Nab2': [1509, 1695, 1774, 1823, 1864, 1970, 2018, 2999, 3033, 3405, 3419, 3462, 3557, 3628, 3687, 3749, 3761, 3786, 3817, 3905], '(nuclear': [1510], 'poly-adenylated': [1511], 'RNA-binding': [1512], '(heterogeneous': [1517], '(17Aitchison': [1521, 3420, 3694, 3765, 3927], 'J.D.': [1522, 1539, 1577, 1649, 1706, 1749, 2696, 3009, 3421, 3438, 3536, 3695, 3712, 3766, 3928, 3955], 'Rout': [1525, 2492, 2691, 3424, 3698, 3769, 3931, 3950], 'M.P.': [1526, 2692, 3425, 3699, 3770, 3932, 3951], '274:': [1529, 1544, 1582, 1654, 1711, 1754, 3428, 3443, 3541, 3702, 3717, 3773, 3935], '624-627Crossref': [1530, 3429, 3703, 3774, 3936], '(270)': [1533, 3432, 3706, 3777, 3939], '18Lee': [1536, 3435, 3709], 'D.C.': [1537, 1575, 1647, 1704, 1747, 3436, 3534, 3710], 'Aitchison': [1538, 1576, 1648, 1705, 1748, 2695, 3437, 3535, 3711, 3954], '29031-29037Abstract': [1545, 1583, 1655, 1712, 1755, 3444, 3542, 3718], '(86)': [1553, 1591, 1663, 1720, 1763, 3452, 3550, 3726], 'Previous': [1556], 'work': [1557], 'general': [1561], 'regions': [1562], 'Kap104-mediated': [1571], '(18Lee': [1574, 1646, 1703, 1746, 3533], '19Truant': [1594, 1666], 'R.': [1595, 1667, 2231], 'Fridell': [1596, 1668], 'R.A.': [1597, 1669], 'Benson': [1598, 1670], 'Bogerd': [1600, 1672], 'Cullen': [1602, 1674], 'B.R.': [1603, 1675], '1998;': [1607, 1679], '18:': [1608, 1680], '1449-1458Crossref': [1609, 1681], '(62)': [1612, 1684], 'containing': [1619, 2796, 3045, 3082, 3605], 'RNA': [1621], 'motif': [1623], 'consisting': [1624], 'series': [1627], 'arginine-glycine-glycine': [1629], '(RGG)': [1630], 'repeats': [1631], 'Deletion': [1687], 'CCCH': [1690, 3564], 'zinc': [1691, 3565, 3579, 3593], 'finger': [1692, 3566], 'reduces': [1697], 'vitro': [1702], 'arginine/glycine-rich': [1726], 'carboxyl': [1727], 'terminus': [1728, 3519, 3804, 3823], 'Thus,': [1766], 'although': [1767, 3684], 'domains': [1770], 'determined,': [1781], 'no': [1782], 'identified.': [1789], 'Much': [1790], 'like': [1791], 'involved': [1825], 'mRNA': [1827, 1872], 'biogenesis,': [1828], 'contributing': [1831], 'cleavage': [1833], 'polyadenylation': [1835], 'pre-mRNA': [1837], '3′-ends': [1838], '(20Kessler': [1839], 'M.M.': [1840, 2356], 'Henry': [1841, 2357], 'M.F.': [1842, 2358], 'Shen': [1843, 2359], 'Zhao': [1845, 2361], 'Gross': [1847, 2363], 'Silver': [1849, 2365], 'P.A.': [1850, 2366], 'C.L.': [1852, 2368], 'Genes': [1853, 2369], 'Dev.': [1854, 2370], '1997;': [1855, 2371], '2545-2556Crossref': [1857, 2373], '(198)': [1860, 2376], 'aiding': [1865], 'poly(A)': [1867], 'tail': [1868], 'length': [1869], 'regulation': [1870], 'export': [1873], '(21Hector': [1874], 'Nykamp': [1876], 'K.R.': [1877], 'Dheur': [1878], 'Anderson': [1880], 'J.T.': [1881], 'Non': [1882], 'P.J.': [1883], 'Urbinati': [1884], 'C.R.': [1885], 'Wilson': [1886], 'Minvielle-Sebastia': [1888], 'Swanson': [1890], 'M.S.': [1891], '21:': [1895], '1800-1810Crossref': [1896], '(140)': [1899], '22Green': [1902], 'D.M.': [1903, 2257, 2287, 2684, 3845, 3943], 'Marfatia': [1904, 2258, 2288, 3846], 'K.A.': [1905, 2259, 2289, 3847], 'Crafton': [1906, 2260, 2290, 3848], 'E.B.': [1907, 2261, 2291, 3849], 'X.': [1909, 1911, 2263, 2265, 2293, 2295, 3851, 3853], 'Cheng': [1910, 2264, 2294, 3852], '277:': [1918, 2272, 2302, 3860], '7752-7760Abstract': [1919, 2273, 2303, 3861], '(150)': [1927, 2281, 2311, 3869], 'Interestingly,': [1930], 'related': [1936], 'A1': [1943], '(PBI-BLAST).': [1944], 'goal': [1946], 'study': [1949, 2171, 3344], 'examine': [1952], 'function': [1954, 2992, 3835], 'Examination': [1962], 'primary': [1965, 2953, 2971], 'uncovered': [1971], 'combination': [1980], 'localization,': [1982], 'studies,': [1986, 2671], 'found': [1988], 'less': [2013], 'accumulation': [2024, 4098, 4323], 'Nab2.': [2026], 'results': [2028, 3376, 3394, 4351], 'but': [2040, 3636], 'reveal': [2042], 'likely': [2045, 2083], 'utilizes': [2046], 'additional': [2047], 'mechanisms': [2048], 'recognition.': [2051], 'allowed': [2060], 'us': [2061], 'interrogate': [2063], 'identify': [2068], 'Kap104.': [2073, 3909], 'Therefore,': [2074], 'conclude': [2076], 'prediction': [2080], 'prevalence': [2084], 'cerevisiae.': [2090], 'Strains,': [2091], 'Plasmids,': [2092], 'Chemicals—All': [2094], 'chemicals': [2095], 'were': [2096, 2109, 2115, 2505, 2549, 2578, 2621, 2712, 2736, 2765, 2781, 2806, 2841, 2875, 2901, 2933, 2943, 3035, 3056, 3087, 3127, 3159, 4030, 4047, 4077, 4139, 4186, 4268], 'obtained': [2097], 'United': [2099], 'States': [2100], 'Biological': [2101], 'Sigma': [2103], 'unless': [2104], 'otherwise': [2105], 'noted.': [2106], 'All': [2107, 2163], 'media': [2108], 'prepared': [2110, 2775], 'all': [2112, 3982, 4134], 'DNA': [2113], 'manipulations': [2114], 'performed': [2116], 'according': [2117, 3338], 'standard': [2119], 'procedures': [2120], '(23Adams': [2121], 'Gottschling': [2123], 'D.E.': [2124], 'Kaiser': [2125], 'C.A.': [2126], 'Stearns': [2127], 'Methods': [2129, 3016], 'Yeast': [2131], 'Genetics.': [2132, 2239], 'Cold': [2133, 2138, 2153, 2158], 'Spring': [2134, 2139, 2154, 2159], 'Harbor': [2135, 2155], 'Laboratory': [2136, 2150, 2156], 'Harbor,': [2140, 2160], 'NY1997Google': [2141], '24Sambrook': [2143], 'Russell': [2145], 'Molecular': [2147], 'Cloning:': [2148], 'Manual.': [2151], '3rd.': [2152], 'NY2001Google': [2161], 'strains': [2165], 'plasmids': [2167, 2179, 2797], 'described': [2173], 'Table': [2175], '1.TABLE': [2176], '1Strains': [2177], 'studyStrain/PlasmidDescriptionOriginFY23': [2183], '(ACY192)Wild': [2184], 'type,': [2185], 'MATa': [2186, 2220], 'ura3-52': [2187], 'leu2Δ1': [2188], 'trp1(30Winston': [2189], 'Dollard': [2191], 'Ricupero-Hovasse': [2193], 'S.L.': [2194], 'Yeast.': [2195], '1995;': [2196], '53-55Crossref': [2198], '(784)': [2201], 'Scholar)ESY41-4C': [2203], '(ACY251)ΔKAP104::HIS3': [2204], '[KAP104': [2205], 'CEN': [2206, 2217, 2228, 2254, 2284, 2316, 2379, 2383, 2402, 2407, 2412, 2424, 2429, 2434, 2439, 2445, 2451, 2457, 2463, 2468, 2480, 2485], 'URA3': [2207, 2218, 2229, 2250, 2317, 2354, 2380, 2391, 2398, 2420, 2446, 2452, 2458, 2464, 2476, 3051], 'AMPR],': [2208, 2219], 'MATα': [2209], 'ade': [2210, 2223], 'trp': [2211], 'leu': [2212, 2221], 'hisP.': [2213], 'SilverACY427ΔNAB2::HIS3': [2215], '[NAB2': [2216], 'lys': [2222], 'hisThis': [2224], 'studySVL182/PSY1224': [2225], '(ACY1571)ΔHRP1::HIS3': [2226], '[HRP1': [2227], 'AMPR]S.': [2230], 'ValentinipRS315': [2232], '(pAC3)CEN': [2233], 'LEU2': [2234, 2255, 2285, 2384, 2403, 2408, 2413, 2425, 2430, 2435, 2440, 2469, 2481, 2486], 'AMPR(31Sikorski': [2235], 'R.S.': [2236], 'Hieter': [2237], 'P.': [2238, 2491], '1989;': [2240], '122:': [2241], '19-27Crossref': [2242], 'Scholar)pPS808': [2245], '(pAC23)GFP,': [2246], 'GAL1-10': [2247, 2351, 2388, 2395, 2417, 2473], 'promoter,': [2248, 2315, 2352, 2389, 2396, 2418, 2444, 2450, 2456, 2462, 2474], '2μ': [2249, 2353, 2390, 2397, 2419, 2475], 'AMPRP.': [2251], 'SilverpAC717NAB2,': [2253], 'AMPR(22Green': [2256, 2286], 'Scholar)pAC753NAB2-GFP,': [2283], 'Scholar)pAC1069GFP-GFP,': [2313], 'MET25': [2314, 2443, 2449, 2455, 2461, 2619], 'AMPR(32Hodel': [2318], 'Harreman': [2320], 'Pulliam': [2322], 'K.F.': [2323], 'Harben': [2324], 'M.E.': [2325], 'Holmes': [2326], 'J.S.': [2327, 3363], 'Hodel': [2328], 'M.R.': [2329], 'Berland': [2330], 'K.M.': [2331], '281:': [2338], '23545-23556Abstract': [2339], '(81)': [2347], 'Scholar)pPS1358': [2349], '(pAC1725)GFP-HRP1,': [2350], 'AMPR(20Kessler': [2355], 'Scholar)pAC2023NOP1-GFP,': [2378], 'AMPRThis': [2381, 2385, 2392, 2399, 2404, 2409, 2414, 2421, 2426, 2431, 2436, 2441, 2447, 2453, 2459, 2465, 2470, 2477, 2482, 2487], 'studypAC2325HRP1,': [2382], 'studypAC2329GFP-HRP1': [2386], 'R525A/P531A/Y532A,': [2387, 2401], 'studypAC2330GFP-HRP1': [2393], 'P531A/Y532A,': [2394, 2406], 'studypAC2344HRP1': [2400], 'studypAC2345HRP1': [2405], 'studypAC2440HRP1': [2410], 'Y532V,': [2411, 2416], 'studypAC2441GFP-HRP1': [2415], 'studypAC2442NAB2': [2422], 'P332A,': [2423], 'studypAC2443NAB2-GFP': [2427], 'PV332A,': [2428], 'studypAC2444NAB2': [2432], 'P407A,': [2433, 2438], 'studypAC2445NAB2-GFP': [2437], 'studypAC2451GFP-GFP-HRP1-(503-534),': [2442], 'studypAC2452GFP-GFP-HRP1-(522-534),': [2448], 'studypAC2472GFP-GFP-NAB2-(320-333),': [2454], 'studypAC2473GFP-GFP-NAB2-(389-408),': [2460], 'studypAC2527NAB2': [2466], 'P332A/P407A,': [2467, 2479], 'studypAC2528GFP-HRP1': [2471], 'P531A,': [2472, 2484], 'studypAC2538NAB2-GFP': [2478], 'studypAC2539HRP1': [2483], 'studypAC2551GST-KAP104,': [2488], 'pGEX-6P3': [2489], 'AMPRM.': [2490], 'Open': [2493], 'table': [2494, 3392], 'tab': [2498], 'Microscopy—Green': [2499], '(GFP)': [2502], 'fusion': [2503, 2681, 2838, 2968, 3350, 3828, 4295], 'localized': [2506, 3330, 3880, 3893, 3969, 4048, 4057, 4109, 4310, 4345], 'live': [2508], 'cells': [2509, 2541, 2718, 2732, 2794, 3044, 3103, 3793, 4051, 4192], 'direct': [2511], 'fluorescence': [2512], 'microscopy.': [2513], 'GFP': [2515, 3349, 3800, 3825, 4266], 'signal': [2516], 'visualized': [2518], 'GFP-optimized': [2521], 'filter': [2522], '(Chroma': [2523], 'Technology)': [2524], 'on': [2525, 2935, 3070, 3077, 3397, 3763, 3908], 'Olympus': [2527], 'BX60': [2528], 'epifluorescence': [2529], 'microscope': [2530], 'equipped': [2531], 'Photometrics': [2534], 'Quantix': [2535], 'digital': [2536], 'camera.': [2537], 'experiments,': [2540], 'expressing': [2542, 2569, 2612, 2719, 2733, 4193], 'genes': [2543, 2570, 2613], 'expressed': [2544, 2674, 4140], 'own': [2547], 'promoters': [2548], 'grown': [2550, 2560, 2579, 2622, 2807, 2821, 3057, 3128], 'overnight': [2551, 2580, 2623, 2808, 2822, 2903, 3129], 'selective': [2553, 2582, 2625, 2810, 2824, 3078], 'medium,': [2554, 2558, 2626], 'diluted': [2555, 3064, 3138], 'fresh': [2557, 2594, 2631], '3': [2562], 'h': [2563, 2606, 2639], 'prior': [2564, 2607, 2640], 'studies.': [2567, 2610, 2643], 'Cells': [2568, 2611, 2840, 3158], 'under': [2571, 2614], 'control': [2573, 2616, 3071], 'GAL1–10': [2576], 'promoter': [2577, 2620], 'medium': [2583, 2595, 2632, 2811, 2825], 'glucose': [2585, 2813], 'sugar': [2588, 2600, 2816, 2830], 'source,': [2589, 2601, 2817], 'pelleted,': [2590, 2627, 2818], 'washed,': [2591, 2628, 2819], 'resuspended': [2592, 2629, 2737], 'galactose': [2597, 2827], 'induced': [2603, 2636], '6': [2605], 'lacking': [2633, 4374], 'methionine,': [2634], '5': [2638, 2853], 'Experiments': [2644], 'ΔKAP104': [2647, 3792, 3886, 3977, 3988], 'deletion': [2648], 'strain': [2649], '(ACY251)': [2650], 'initial': [2653], 'growth': [2654, 3100, 3147], 'phase': [2655], 'five': [2657, 3658], 'nights': [2658], 'reach': [2660], 'appropriate': [2662], 'cell': [2663], 'density.': [2664], 'Vitro': [2666], 'GST': [2667, 2680], 'Binding': [2668], 'Assay—For': [2669], 'Escherichia': [2676], 'coli': [2677, 2731], '(25Leslie': [2683], 'W.': [2686, 3945], 'Timney': [2687, 3946], 'B.L.': [2688, 3947], 'Chait': [2689, 3948], 'B.T.': [2690, 3949], 'Wozniak': [2693, 3952], 'R.W.': [2694, 3361, 3953], '24:': [2701, 3960], '8487-8503Crossref': [2702, 3961], '(46)': [2705, 3964], 'GST-Kap104': [2710, 2728, 2734, 2898, 2984, 4169, 4184, 4207], 'beads': [2711, 2777, 4185], 'incubated': [2713, 2770, 2902, 3088, 3160, 4187], 'lysate': [2716, 2893, 4190], 'wild-type': [2720, 2793, 2799, 3028, 3047, 3117, 3790, 3873, 3975, 4050, 4103, 4174, 4195], 'GFP-Hrp1': [2721, 2800, 2804, 2837, 2967, 3876, 3889, 4041, 4045, 4055, 4081, 4107, 4137, 4177, 4196, 4199, 4203, 4210], 'R525A/P531A/Y532A': [2723, 2803, 4198, 4209], 'GFP-Hrp1.': [2724], 'To': [2725, 2789, 4148, 4227], 'prepare': [2726, 2790], 'beads,': [2729], '(pAC2551)': [2735], 'PBS': [2739], 'supplemented': [2740, 2866], '0.5': [2742], 'mm': [2743, 2758, 2854, 2923], 'phenylmethylsulfonyl': [2744], 'fluoride,': [2745], 'PLAC': [2746], '(3': [2747], 'mg/ml': [2748], 'pepstatin': [2751], 'A,': [2752], 'leupeptin,': [2753], 'aprotinin,': [2754], 'chymostatin),': [2755], '2': [2757, 3583], 'β-mercaptoethanol': [2759], 'lysed': [2761], 'sonication.': [2763], 'Lysates': [2764, 2874], 'cleared': [2766, 2876], 'centrifugation': [2768], '200': [2772], 'μl': [2773, 2896], 'glutathione-Sepharose': [2776], '(GE': [2778], 'Healthcare).': [2779], 'Beads': [2780], 'collected': [2782, 2842], 'washed': [2784, 2844, 2916], 'three': [2785, 2917], 'times': [2786, 2918], 'PBS.': [2788, 2928], 'lysates,': [2792], '(ACY192)': [2795], 'encoding': [2798, 3027], '(pAC1725)': [2801], '(pAC2329)': [2805], 'source': [2831], 'induce': [2833], 'twice': [2845], 'dH2O': [2847], 'once': [2849, 2926], 'PBSMT': [2851, 2865, 2920], '(PBS,': [2852], 'MgCl2,': [2855], '0.5%': [2856], 'Triton': [2857], 'X-100).': [2858], 'Glass': [2859], 'bead': [2860, 2899], 'lysis': [2861], 'conducted': [2863], 'protease': [2868], 'inhibitors': [2869], '(phenylmethylsulfonyl': [2870], 'fluoride': [2871], 'PLAC).': [2873], 'centrifugation,': [2878], 'total': [2880, 2891], 'concentration': [2882], 'assessed': [2884, 3788], 'Bradford': [2886], 'assay.': [2887], 'milligram': [2889], '50': [2895], 'slurry': [2900], 'agitation.': [2905], 'unbound': [2907, 2931], 'fraction': [2908, 2914], 'removed,': [2910], '+': [2921], '500': [2922], 'NaCl': [2924], 'Bound': [2929], 'samples': [2932], 'loaded': [2934], '10%': [2937], 'denaturing': [2938], 'SDS-PAGE': [2939], 'gel.': [2940], 'transferred': [2944], 'nitrocellulose': [2947], 'probed': [2950], 'α-GFP': [2954], '(rabbit,': [2955], '1:3,000': [2956], 'dilution)': [2957], 'secondary': [2959, 2976], 'α-rabbit': [2960], 'HRP-conjugated': [2961, 2978], '(1:5,000)': [2962, 2979], 'antibodies': [2963, 2980], 'detect': [2965, 2982], 'α-GST': [2972], '(mouse,': [2973], '1:5,000)': [2974], 'α-mouse': [2977], 'protein.': [2985], 'Vivo': [2987], 'Functional': [2988], 'Analyses—The': [2989], 'variants': [3000, 4046], 'tested': [3002, 4166], 'plasmid': [3005, 3111], 'shuffle': [3006, 3112], 'technique': [3007], '(26Boeke': [3008], 'Trueheart': [3010], 'Natsoulis': [3012], 'Fink': [3014], 'G.R.': [3015], 'Enzymol.': [3017], '1987;': [3018], '154:': [3019], '164-175Crossref': [3020], '(1075)': [3023], 'Plasmids': [3026], 'variant': [3030], 'transformed': [3036], 'ΔHRP1': [3039], '(ACY1571)': [3040], 'ΔNAB2': [3042], '(ACY427)': [3043], 'HRP1': [3048], 'NAB2': [3050], 'maintenance': [3052], 'plasmid.': [3053], 'Single': [3054], 'transformants': [3055], 'saturation': [3059], 'liquid': [3061], 'culture,': [3062], 'serially': [3063], '(1:10)': [3065], 'dH2O,': [3067], 'spotted': [3069], 'ura–': [3072], 'leu–': [3073, 3079], 'glu': [3074, 3080], 'plates': [3075, 3081], 'acid': [3084, 4028], '(5-FOA).': [3085], 'Plates': [3086], '18,': [3090], '25,': [3091], '30,': [3092], '37': [3094, 3162], '°C': [3095, 3108, 3163], '3–5': [3097], 'days.': [3098], 'curve': [3101], 'analysis,': [3102], 'picked': [3104], '25': [3107, 3131], '5-FOA': [3109], 'plate': [3113], 'express': [3115], 'P531A': [3119], 'copy': [3124], '°C,': [3132], 'normalized': [3133], 'equal': [3135, 4143], 'starting': [3136], 'concentrations,': [3137], '1:10': [3139], '96-well': [3142], 'plate,': [3143], 'monitored': [3145], 'ultra': [3152], 'microplate': [3153], 'reader': [3154], '(Bio-Tek': [3155], 'Instruments,': [3156], 'Inc.).': [3157], 'shaking,': [3165], 'optical': [3168], 'density': [3169], 'measured': [3171], '600': [3173], 'nm': [3174], 'every': [3175], '30': [3176], 'min.': [3177], 'Bioinformatics—A': [3178], 'custom': [3179], 'library': [3180, 3266, 3351], 'constructed': [3182], 'profiles:': [3186], '(LIMHFYVPQ)-(GAS)-(LIMHFYVPRQK)-(LIMHFYVPRQK)-X(7,12)-(RKH)-X(2,5)-PY;': [3220], '(KR)-X(0,2)-(KR)-(KR)-X(3,10)-(RKH)-X(1,5)-PY;': [3253], 'PY-core': [3260, 3646, 4035, 4247, 4316, 4373], '(RKH)-X(2,5)-PY.': [3264], 'This': [3265, 3674, 3910], 'ScanProsite': [3271], '(27Gattiker': [3272], 'Gasteiger': [3274], 'Bairoch': [3276], 'Appl.': [3278], 'Bioinformatics.': [3279], '1:': [3281], '107-108PubMed': [3282], 'query': [3286], 'data': [3288, 3303], 'sets:': [3289], 'represented': [3293], '5,850': [3296], 'GenBank™': [3302], 'base': [3304], '(28Benson': [3305], 'D.A.': [3306], 'Karsch-Mizrachi': [3307], 'I.': [3308], 'Lipman': [3309], 'D.J.': [3310], 'Ostell': [3311], 'Wheeler': [3313], 'D.L.': [3314], 'Nucleic': [3315], 'Acids': [3316], 'Res.': [3317], '35:': [3319], 'D21-D25Crossref': [3320], '(384)': [3323], '1,515': [3328], 'nucleolus': [3337], 'comprehensive': [3341], 'subcellular': [3342], 'global': [3347], '(29Huh': [3352], 'W.K.': [3353], 'Falvo': [3354], 'J.V.': [3355], 'Gerke': [3356], 'Carroll': [3358], 'A.S.': [3359], 'Howson': [3360], 'Weissman': [3362], "O'Shea": [3364], 'E.K.': [3365], '425:': [3368], '686-691Crossref': [3369], '(3306)': [3372], 'summarized': [3378], 'Venn': [3381], 'diagram': [3382], 'corresponding': [3384], 'percentages': [3385], 'tabulated': [3386], 'chart.': [3389], 'detailed': [3391], 'presented': [3396], 'laboratory': [3400], 'Web': [3401], 'site.': [3402], 'Contain': [3406], 'Sequences—Scanning': [3408], 'revealed': [3455], 'contains': [3458, 3463], 'similar': [3468], 'established': [3471, 3690], '(Fig.': [3504, 3794, 4052, 4200, 4263], '1A).': [3505], '(residues': [3511, 3571, 3584, 4248, 4257], '506–532)': [3512], 'located': [3514, 3587], 'extreme': [3517], 'C': [3518, 3822], 'previously': [3526, 3831], 'shown': [3527, 3833], 'be': [3529], 'domain.': [3567], '1': [3570], '320–333)': [3572], 'lies': [3573], 'between': [3574, 3588, 4161], 'second': [3576], 'third': [3578], 'fingers,': [3580], '389–408)': [3585], 'fourth': [3590], 'fifth': [3592], 'finger.': [3594], 'very': [3599, 4337], 'resembles': [3601], 'mammalian': [3603], 'core': [3607], 'preceded': [3614], 'stretch': [3618], 'acids.': [3621], 'stretches,': [3635], 'lysine': [3652], 'residue': [3653, 3679], 'followed': [3654], 'valine,': [3665], 'yielding': [3666], 'PV': [3667], 'rather': [3668], 'eponymous': [3671], 'PY': [3672, 4065, 4074], 'sequence.': [3673, 3816], 'final': [3678], 'raises': [3680], 'possibility': [3682], 'that,': [3683], 'sets': [3737], 'perhaps': [3741], 'fundamentally': [3744], 'ways.': [3746], 'Are': [3750], 'Imported': [3751], 'Kap104—To': [3753], 'verify': [3754], 'depends': [3762], 'GFP-tagged': [3783, 3915], '1B).': [3795], 'tagged': [3798, 3819], 'N': [3803], 'preserve': [3809], 'location': [3812], 'because': [3826], 'localize': [3840], '(22Green': [3844], 'Nab2-GFP': [3878, 3891], 'nucleus;': [3883, 4060], 'throughout': [3894], 'cell,': [3896], 'indicating': [3897, 4087], 'dependent': [3907], 'experiment': [3911], 'demonstrates': [3913], 'versions': [3916], 'valid': [3921], 'reporters.': [3922], 'non-Kap104-dependent': [3924], 'Nop1-GFP': [3926], '25Leslie': [3942], 'properly': [3968], 'showing': [3979], 'impaired': [3986], 'cells.': [3989], 'Sequence': [3992], 'Is': [3995, 4003], 'Necessary': [3996, 4004], 'Sufficient': [3998], 'Import': [4001], 'Binding—To': [4007], 'test': [4008, 4228], 'whether': [4009, 4150, 4167, 4229], 'changes': [4029], 'created': [4031], 'resulting': [4044], '2A).': [4053], 'Wild-type': [4054, 4202], 'when': [4062], '(P531A/Y532A)': [4066], 'arginine': [4071], '(R525A/P531A/Y532A)': [4075], 'changed': [4078], 'alanine,': [4080], 'mislocalized': [4083], 'Hrp1.': [4100], 'Both': [4101], 'mutant': [4106, 4176], 'partially': [4108], 'discrete': [4111], 'puncta.': [4112], 'accumulations': [4114], 'do': [4115], 'seem': [4117], 'correlate': [4119], 'cytoplasmic': [4124, 4327], 'bodies': [4125], 'cause': [4128], 'unknown.': [4130], 'Immunoblotting': [4131], 'verified': [4132], 'approximately': [4142], 'levels': [4144], '(data': [4145], 'shown).': [4147], 'determine': [4149], 'recombinant': [4168], 'could': [4170], 'lysate.': [4180], 'experiment,': [4183], '2B).': [4201], 'robustly': [4204], 'whereas': [4208], 'greatly': [4212], 'reduced': [4213], 'binding,': [4214], 'demonstrating': [4215], 'important': [4223], 'binding.': [4226], 'non-nuclear': [4242], '522–534)': [4249], 'entire': [4252], '503–534)': [4258], 'fused': [4260, 4317, 4333], 'GFP-GFP': [4262, 4306, 4319, 4335], '2C).': [4264], 'Two': [4265], 'molecules': [4267], 'create': [4271], 'too': [4276], 'efficiently': [4279], 'diffuse': [4280], 'Accordingly,': [4288], 'accumulate': [4290], 'must': [4297], 'utilize': [4298], 'active': [4300], 'transport.': [4303], 'As': [4304], 'expected,': [4305], 'alone': [4307], 'mainly': [4309], 'cytoplasm.': [4313], 'significant': [4321], 'remaining': [4326], 'signal.': [4328], 'complete': [4330], 'mediated': [4336], 'efficient': [4338], 'reporter': [4343], 'exclusively': [4346], 'indicate': [4352], 'medi': [4382]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2054739800', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 3}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 5}], 'updated_date': '2024-12-16T14:37:54.580696', 'created_date': '2016-06-24'}