Title: Adenoid tissue lymphocyte subpopulations - evaluation of a quantitative analysis with flow cytometry
Abstract: APMISVolume 101, Issue 7-12 p. 551-556 Adenoid tissue lymphocyte subpopulations — evaluation of a quantitative analysis with flow cytometry CLAES HEMLIN, Corresponding Author CLAES HEMLIN Departments of Otorhinolaryngology, Karolinska Hospital, Stockholm, SwedenDepartment of Otorhinolaryngology, Karolinska Hospital, S-104 41 Stockholm, Sweden.Search for more papers by this authorCHRISTER CARENFELT, CHRISTER CARENFELT Departments of Otorhinolaryngology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this authorGUNILLA HALLDÉN, GUNILLA HALLDÉN Departments of Clinical Immunology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this authorJAN HED, JAN HED Departments of Clinical Immunology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this authorANNIKA SCHEYNIUS, ANNIKA SCHEYNIUS Departments of Clinical Immunology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this author CLAES HEMLIN, Corresponding Author CLAES HEMLIN Departments of Otorhinolaryngology, Karolinska Hospital, Stockholm, SwedenDepartment of Otorhinolaryngology, Karolinska Hospital, S-104 41 Stockholm, Sweden.Search for more papers by this authorCHRISTER CARENFELT, CHRISTER CARENFELT Departments of Otorhinolaryngology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this authorGUNILLA HALLDÉN, GUNILLA HALLDÉN Departments of Clinical Immunology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this authorJAN HED, JAN HED Departments of Clinical Immunology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this authorANNIKA SCHEYNIUS, ANNIKA SCHEYNIUS Departments of Clinical Immunology, Karolinska Hospital, Stockholm, SwedenSearch for more papers by this author First published: July 1993 https://doi.org/10.1111/j.1699-0463.1993.tb00145.xCitations: 7AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Secretory otitis media (SOM) is a common childhood disease without a completely clarified etiology. A chronic inflammatory condition in the nasopharynx, presumably caused by an increased bacterial load, is one factor of probable etiological importance. In the present study a flow cytometric method for analysis of adenoid lymphoid cell populations was developed to facilitate quantitative comparisons between children with SOM and children without ear disease. Adenoids removed from 18 children due to adenoid hyperplasia and obstructive symptoms were studied. Results of the flow cytometric analysis correlated well with the findings from immunohistological studies of five of the adenoids. PCA-1 and CD25 were found to be good markers of increased cellular activity after non-specific stimulation in cell culture. It is concluded that the flow cytometric method is suitable for further quantitative analysis of adenoid tissue. REFERENCES 1 Anderson, K. C., Park, E. K., Bates, M. P., Leonard, R. C. F., Hardy, R., Schlossman, S. F. & Nadler, L. M.: Antigens on human plasma cells identified by monoclonal antibodies. J. Immunol. 730: 1132–1138, 1983. 2 Bernstein, J. M., Drya, D. M., Loos, B. G. & Dickinson, D. P.: Restriction fragment mapping of nontypable haemophilus influenzae: a new tool to study this middle ear pathogen. Otolaryngol. Head Neck Surg 700: 200–206, 1989. 3 Cantrell, D. S. & Smith, K. A.: Transient expression of interleukin 2 receptors. Consequences for T cell growth. J. Exp. Med. 158(6): 1895–1911, 1983. 4 Gates, G. A., Avery, C. A. & Prihoda, T. J.: Effect of adenoidectomy upon children with chronic otitis media with effusion. 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Publication Year: 1993
Publication Date: 1993-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 8
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