Title: Human memory T cell differentiation into Th2-like effector cells is dependent on IL-4 and CD28 stimulation and inhibited by TCR ligation
Abstract: European Journal of ImmunologyVolume 28, Issue 8 p. 2517-2529 ArticleFree Access Human memory T cell differentiation into Th2-like effector cells is dependent on IL-4 and CD28 stimulation and inhibited by TCR ligation Hendrik Schulze-Koops, Hendrik Schulze-Koops University of Texas Southwestern Medical Center, Department of Intermal Medicine, Dallas, USASearch for more papers by this authorPeter E. Lipsky, Peter E. Lipsky University of Texas Southwestern Medical Center, Department of Intermal Medicine, Dallas, USASearch for more papers by this authorLaurie S. Davis, Laurie S. Davis University of Texas Southwestern Medical Center, Department of Intermal Medicine, Dallas, USASearch for more papers by this author Hendrik Schulze-Koops, Hendrik Schulze-Koops University of Texas Southwestern Medical Center, Department of Intermal Medicine, Dallas, USASearch for more papers by this authorPeter E. Lipsky, Peter E. Lipsky University of Texas Southwestern Medical Center, Department of Intermal Medicine, Dallas, USASearch for more papers by this authorLaurie S. Davis, Laurie S. Davis University of Texas Southwestern Medical Center, Department of Intermal Medicine, Dallas, USASearch for more papers by this author First published: 28 March 2006 https://doi.org/10.1002/(SICI)1521-4141(199808)28:08<2517::AID-IMMU2517>3.0.CO;2-5Citations: 27AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Freshly isolated memory T cells primarily produced IL-2 and small amounts of IL-4 and IFN-γ after stimulation in vitro. Priming for 5 days in vitro with anti-CD28 monoclonal antibodies (mAb) alone markedly increased production of IL-4. In comparison to fresh cells, the increase in the amount of IL-4 secreted reflected a marked increase in the number of IL-4-producing cells. Stimulation with immobilized anti-CD3 mAb during priming limited subsequent IL-4 production. By contrast, IFN-γ production from in vitro primed memory T cells was directly correlated to the concentration of priming anti-CD3 mAb. IL-2 production by all restimulated cells was decreased. The differentiation of IL-4-producing cells could be blocked by antibody to IL-4 and enhanced by the addition of recombinant IL-4 as well as antibody to IFN-γ. 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