Title: Platelet release reaction and aggregation induced by canatoxin, a convulsant protein: evidence for the involvement of the platelet lipoxygenase pathway
Abstract: British Journal of PharmacologyVolume 84, Issue 2 p. 551-560 Free Access Platelet release reaction and aggregation induced by canatoxin, a convulsant protein: evidence for the involvement of the platelet lipoxygenase pathway C.R. Carlini, C.R. Carlini Department of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilSearch for more papers by this authorJ.A. Guimarães, Corresponding Author J.A. Guimarães Department of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilDepartment of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilSearch for more papers by this authorJ.M.C. Ribeiro, J.M.C. Ribeiro Department of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilSearch for more papers by this author C.R. Carlini, C.R. Carlini Department of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilSearch for more papers by this authorJ.A. Guimarães, Corresponding Author J.A. Guimarães Department of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilDepartment of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilSearch for more papers by this authorJ.M.C. Ribeiro, J.M.C. Ribeiro Department of Biochemistry-ICB, CCS, Universidade Federal do Rio de Janeiro, C.P. 68041, 21.910 Rio de Janeiro, BrazilSearch for more papers by this author First published: February 1985 https://doi.org/10.1111/j.1476-5381.1985.tb12940.xCitations: 45AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract 1 Canatoxin is a toxic protein isolated from Canavalia ensiformis seeds. It induces death preceded by convulsions of spinal cord origin and also produces in vitro aggregation of platelets in rabbit, human and guinea-pig plasma. The aggregating effect is dose-dependent at nanomolar concentrations. 2 Rabbit platelets pretreated with canatoxin became refractory to a second exposure to this protein or to collagen, but were still responsive to ADP, Paf-acether or arachidonic acid. [14C]-5-hydroxytryptamine was released from pre-labelled platelets on stimulation with canatoxin. 3 Washed rabbit platelets, but not thrombin-degranulated ones, aggregated on stimulation with canatoxin provided that fibrinogen was added before the toxin. 4 Canatoxin's pro-aggregating activity was inhibited by mepacrine, EDTA, caffeine, prostacyclin, adenosine monophosphate and also by the ADP scavenger system, creatine phosphokinase/creatine phosphate. Furthermore, 3-amino-l-[m-(trifluoromethyl)-phenyl]-2-pyrazoline (BW 755C), eicosatetraynoic acid (ETYA) and nordihydroguaiaretic acid (NDGA) were potent inhibitors of canatoxin-induced aggregation. In contrast, no inhibition was seen with indomethacin. 5 The data indicate that canatoxin is mainly a release-reaction-promoting agent, being devoid of any direct aggregating activity. Thus the aggregation is totally dependent on the release of ADP. Furthermore, canatoxin-induced platelet activation is probably dependent on platelet phospholipase A2 and lipoxygenase activity but is not dependent on cyclo-oxygenase products or the release of Paf-acether. References AHARONY, D., SMITH, J.B. & SILVER, M.J. (1982). Regulation of arachidonate-induced platelet aggregation by the lipoxygenase product, 12-hydroxyperoxyeicosatetraenoic acid. Biochim. biophys. Acta, 718, 193– 200. ARDLIE, N.G., PACKHAM, M.A. & MUSTARD, J.F. (1970). 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