Title: Kinetics of the hydrolysis of 5-bromo-4-chloroindol-3-yl β-d-gluocpyranoside by almond emulsin
Abstract: Abstract A kinetic study has been made of the hydrolysis of 5-bromo-4-chloroindol-3-yl β- d -glucopyranoside (1) by almond emulsin. The rate of hydrolysis depends on the nature of the buffer, and decreases in the following order of buffers: phosphate τ acetate τ citrate τ “Tris” hydrochloride. The rate in phosphate buffer was found to pass through a sharp maximum at pH 6.23, and detailed studies were made at pH 6.10 to 6.75 for a range of substrate concentrations and temperatures. Up to a substrate concentration of 500 m m , the Michaelis-Menten law is obeyed accurately over the range of pH investigated. At higher concentrations of substrate, there is some falling off of the rate. The Km for 1, calculated from a Lineweaver-Burk plot, is 1.38 m m . The average energy of activation is 12.4 kcal/mol, which is within the range reported for the hydrolysis of other aryl β- d -glucopyranosides by this enzyme. The study demonstrates the potential utility of the indigogenic principle in the evaluation of the kinetic parameters of other hydrolytic enzymes.
Publication Year: 1969
Publication Date: 1969-03-01
Language: en
Type: article
Indexed In: ['crossref']
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